Chris Lawson Science Director, Biopolymers

AlgalPolysaccharideWorkshop,14-15thJuly2016

CarbosynthGlycomix

Tobealeadingsupplierofcarbohydratesand

nucleosidestoR&Dinthepharmaceutical&

biotechnologyindustries

Tobeaproviderofsolutionstofood&bioscienceR&Dthrough

structuralfunctionalknowledgeandprocess

development

2

Carbosynth&Glycomix

Combinedweofferbothcarbohydrateproducts&solutions

fortheirapplicationsinfood&bioscienceR&D

OurScienceBase�  Scien6ficLeadership&Exper6se:� AComprehensiveRangeofproducts

� Monoandoligosaccharidesynthesis� Polysaccharidechemistry&biochemistry� Theanalysisoflow&highMWcarbohydratestructures

� Thestructure,func6onandapplica6onofcarbohydrates

� Processdevelopment&scaleup

Polysaccharidesinliving6ssues

5

AlgaeMicrobesFruitsSeedsAnimalTissuesFungi/Yeasts/

PolysaccharidesintheServiceofMan�  Infoods:texturemodifiers,gelling&suspensionagents�  Inhealthfoods:Lowsolidsbulkingagentsforloweringcalories,cholesterol,bloodglucoseetc

�  Inpharmaceu6cals:Func6onaltexturemodifiers,drugencapsula6on,gellingandsuspensionaids,drugs(heparin,pentosanpolysulphate)

�  Incosme6cs:creams,waterbinders,suspensionaids�  Inindustrialapplica6ons:rheology,gela6on,suspension(highsolidsoildrillingmuds,waterbasedpaint,frackingfluids,cementretarda6onetc)

PolysaccharideCovalentStructure•  Degrada6on(sugarresidues)•  Methyla6on(linkageanalysis)•  Par6alHydrolysis(chemical,enzyma6c)(linkage,branching)•  SmithDegrada6on,AlkaliPeeling,reduc6on/oxida6on

(linkage,branching)•  Spectroscopy(NMR,FTIR,MS,Ramanetc)(linkage

configura6on,anomericity,branching)•  Chromatography(purity,molecularweight)•  SEC-Malls,LightScaVering,Ultracentrifuge(molecular

weight)

XanthanGum–CovalentStructure

8

ReesD.A.etal,Carbohydr.Res.,46,245(1976)Lawson,C.J.,Symes,K.C.,CarbohydrRes.,58,433(1977)

PolysaccharideSecondary&Ter6aryStructure

� CovalentStructure� X-rayCrystallography� NMRSpectroscopy� CircularDichroism� PolarisedInfrared� Op6calRotaryDispersion� ComputerModelBuilding

DoubleHelixofκ-carrageenan

10

DeaI.C.M,McKinnonA.A,ReesD.A.,J.Mol.Biol.,68,153(1972)LawsonC.JandReesD.A.,Nature,227,392(1970)

CarrageenanGela6onMechanism

11

RobinsonG.,MorrisE.R.,ReesD.A.,J.Chem.Soc.,Chem.Commun.,4,152(1980)

1970’sBinaryPolysaccharideSynergyDescribed

DeaI.C.M.,MorrisE.R.,ReesD.A.,&WelshE.J.,CarbohydRes,57,249(1977)

2010,TernaryPolysaccharideSynergyExaminedintheUltracentrifuge

Ananaly6calultracentrifugestudyonternarymixturesofkonjacglucomannansupplementedwithsodiumalginateandxanthangum.

AbdelhameedA.S.,AngS.,MorrisG.A.,LawsonC.,GahlerR.,WoodS.AndHardingS.E.CarbohydratePolymers,81,145(2010)

ReadyMadeCovalentLinksfromPolysaccharides

14

Carrageenan

Galα1-3GalThealpha-galepitopeandthean6-Galan6bodyinxenotransplanta6onandincancerimmunotherapy

Reinves6ga6onoftheAcetolysisProductsofCarrageenan,Revisionofthestructureofα-1-3-GalactotrioseandaFurtherExampleoftheReverseSpecifici6esofGlycosideHydrolysisand

Acetolysis.LawsonC.J.ReesD.A.,J.Chem.Soc(1968)1301

Chris Lawson Science Director, Biopolymers

AlgalPolysaccharideWorkshop,14-15thJuly2016