choosing the appropriate camera “resolving” the application
TRANSCRIPT
![Page 1: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/1.jpg)
Choosing the Appropriate Camera
“Resolving” the Application
![Page 2: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/2.jpg)
The Appropriate Camera
•If you know the application AND the product, you will win every time
•If you know either the application or the product, you may win half the time
•If don’t know either, you will lose
![Page 3: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/3.jpg)
How do I Select the Correct Product?
•Guess
•Pick The Cheapest
•Pick the Most Expensive
•Call Scott Monroe
•“Resolve” the Application
![Page 4: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/4.jpg)
Resolving the Application:
Provide the Appropriate Resolution for the Application
![Page 5: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/5.jpg)
SpatialSpatial TemporalTemporal
IntensityIntensity
Appropriate “Resolution”
![Page 6: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/6.jpg)
Spatial Resolution
SpatialSpatial
![Page 7: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/7.jpg)
Spatial Resolution
Size of pixels
0.61 N.A.
Mag = 2 pixels.?
100 x
1.3 NA
![Page 8: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/8.jpg)
Spatial Resolution
Number of pixels
Field of View !!Field of View !!
![Page 9: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/9.jpg)
Temporal Resolution
TemporalTemporal
![Page 10: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/10.jpg)
Temporal Resolution
•What is your “Real Time”•How much light do you have in a given time interval?
Considerations:
•Digitizer Readout Rate•CCD Architecture•Quantum Efficiency•Read Noise
![Page 11: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/11.jpg)
Temporal Resolution
Region of InterestRegion of Interest Frame TransferFrame Transfer
Small Array SizeSmall Array Size
InterlineInterline
![Page 12: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/12.jpg)
Temporal Resolution
e
e
ICCD
![Page 13: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/13.jpg)
Intensity Resolution
IntensityIntensity
![Page 14: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/14.jpg)
Intensity Resolution
•Dim and Bright sources within the same Image
•Measure small differences in Intensities
•Measure small Fluctuations on a large background
Dynamic Range
![Page 15: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/15.jpg)
Intensity Resolution
electronselectrons
GrayGrayValuesValues
00
40954095
20,000 40,000 80,000
Signal Mapping to the ADC
![Page 16: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/16.jpg)
Best of “Both Worlds”
SpatialSpatial TemporalTemporal
IntensityIntensity
High Spatial and Intensity Resolution
![Page 17: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/17.jpg)
Best of “Both Worlds”
High Spatial and Intensity Resolution
Full Frame AcquisitionFull Frame Acquisition
![Page 18: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/18.jpg)
Best of “Both Worlds”
SpatialSpatial TemporalTemporal
IntensityIntensity
High Temporal and Intensity Resolution
![Page 19: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/19.jpg)
Best of “Both Worlds”
High Temporal and Intensity Resolution
Binning: On Chip Charge CombinationBinning: On Chip Charge Combination
![Page 20: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/20.jpg)
Best of “Both Worlds”
High Temporal and Intensity Resolution
On-Chip Multiplication GainOn-Chip Multiplication Gain
![Page 21: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/21.jpg)
Best of “Both Worlds”
SpatialSpatial TemporalTemporal
IntensityIntensity
High Spatial and Temporal Resolution
![Page 22: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/22.jpg)
Best of “Both Worlds”
High Spatial and Temporal Resolution
( X , Y )( X , Y ) ( X , t )( X , t )
““Shoot and Shift” or “Kinetics Mode”Shoot and Shift” or “Kinetics Mode”
![Page 23: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/23.jpg)
Best of “Both Worlds”
High Spatial and Temporal Resolution
( X , Y )( X , Y ) ( x, y , t )( x, y , t )
““Virtual Chip Mode”Virtual Chip Mode”
![Page 24: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/24.jpg)
Biological Products
![Page 25: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/25.jpg)
Microscopy Products
•Tier 1– CoolSNAPcf Color and Mono
•Tier 2– CoolSNAPES and CoolSNAPHQ
•Tier 3– SenSys, Quantix, Cascade
•Tier 4– MicroMAX Backthinned, I-PentaMAX
![Page 26: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/26.jpg)
Price Points:
1x
4-6x
2x
3x
8x
![Page 27: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/27.jpg)
Biological Products
Camera Type Array Size Pixel Size(m) Bit Depth Feature CoolSNAPcf Interline 1392x1030 4.65 12 Hyper HAD SenSys 1401E Full Frame 1317x1035 6.8 12 ITO CoolSNAPes Interline 1392x1040 6.45 12 Hyper HAD CoolSNAPHQ Interline 1392x1040 6.45 12 Super HAD, EXview MicroMax 512BFT Frame Transfer 512x512 13 16 Back Illuminated Quantix 57 Frame Transfer 535x512 13 12 Back Illuminated MicroMax 1024B Full Frame 1024x1024 13 16 Back Illuminated I-PentaMax ICCD, FT 512x512 15 12 Gen 3 Intensifier Cascade 512F Frame Transfer 512x512 16 16 On-Chip Gain Cascade 512B Frame Transfer 512x512 16 16 On-Chip Gain, BI
![Page 28: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/28.jpg)
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras:CoolSNAP
1.3 Mega PixelsSmall Pixels
20MHzInterline Architecture
![Page 29: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/29.jpg)
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
SenSys
1.4 Mega PixelsSmall Pixels
Full Frame Architecture
![Page 30: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/30.jpg)
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
512BFT
SenSys
Frame Transfer Architecture
16-bit 100kHz, 1MHz
![Page 31: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/31.jpg)
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
512BFT
SenSysQT 57
Frame Transfer Architecture
12-bit, 1-3MHz
![Page 32: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/32.jpg)
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
512BFT
SenSys
1024B
QT 57
16-bit 100kHz, 1MHz
1.0 Mega Pixels
Large pixels
![Page 33: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/33.jpg)
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
512BFT
SenSys
1024B
QT 57 I-PMax
Gen 3 IntensifierFrame Transfer Architecture
![Page 34: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/34.jpg)
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
Roper Scientific Cameras
512BFT
SenSys
1024B
QT 57 I-PMax
On-Chip Multiplication GainFrame Transfer Architecture
512B
16 micron pixels
![Page 35: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/35.jpg)
Where Does Your Application Fit?
?
SpatialSpatial TemporalTemporal
IntensityIntensity
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
![Page 36: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/36.jpg)
Where Does Your Application Fit?
SpatialSpatial TemporalTemporal
IntensityIntensity
Cortical Imaging ala Toga
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
?
![Page 37: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/37.jpg)
Where Does Your Application Fit?
SpatialSpatial TemporalTemporal
IntensityIntensity
Tirf Res/Tirf Fluor
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
?
![Page 38: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/38.jpg)
Where Does Your Application Fit?
SpatialSpatial TemporalTemporal
IntensityIntensity
Embyonic Chick
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
?
![Page 39: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/39.jpg)
Where Does Your Application Fit?
SpatialSpatial TemporalTemporal
IntensityIntensity
Single Molecule Fluor
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
?
![Page 40: Choosing the Appropriate Camera “Resolving” the Application](https://reader035.vdocuments.mx/reader035/viewer/2022081520/5697bf861a28abf838c88061/html5/thumbnails/40.jpg)
Where Does Your Application Fit?
SpatialSpatial TemporalTemporal
IntensityIntensity
Live Cell Fluor
HQ/es/cf
512BFT
SenSys
1024B
QT 57 I-PMax
512B
?