Analysis of NMSU Capabilities relative to ASBC methods of analysis

New Mexico State University

Dr. Rockstraw

CHME498

Zoe Elliott

Table of Contents

List of test methods…………………………………………………………………………………………………………….. 3-24

List of all equipment………………………………………………………………………………………………………….. 24-32

List of budgeted equipment………………………………………………………………………………………………..32-35

References of websites used for budget……………………………………………………………………………..35-40

Beer 1

ASBC Method: sampling

Preparations: Degassing

Method: Beer 1A

Materials and equipment QuantityButane torch 1Sterile pipe cleaners 1Sterile sample flasks 2Sterile Sampling needles 1Alcohol 70% ethyl or isopropyl 1ml Rotary shaker lab line model 3518 or equivalent 1Erlenmeyer flasks 500ml 2Aluminum foil 1Pipetter 10-100µl 1Pipet tips 10-100µl 1Tributyl Phosphate (TBP) Fisher B-404-500 20µl

Description: This method degasses the beer before analyzing it, which is a preparation step for many test methods.

Beer 2

ASBC Method: Specific Gravity

Preparations: remove Particulate matter and decarbonate beer according to degassing matrix

Method: beer 2B

Materials and equipment QuantityDigital density meter 1Hypodermic syringes or autosampler 1

Description: testing for specific gravity of beer with absolutely no particulates or carbonation at 200C.

Beer 3

ASBC Method: Apparent Extract

Preparations: Decarbonate and find specific gravity of beer.

Method: Beer 3A

Materials and equipment QuantityDistilled water 500mlBoiled wort 1Hydrometers 2 (1 for wort and 1 for beer)Hydrometer cylinder glass or stainless steel 1

Description: can use a digital density meter to determine apparent extract of wort and beer as a percent.

Beer 4

ASBC Method: Alcohol

Preparations: Beer 1

Method: Beer 4E

Materials and equipment Quantity95% ethyl alcohol 1Detergent as needed for apparatus 1Conditioning solution 1Calibration solution 1Automatic beer analyzer 1Flasks 1-L 1Graduated cylinder 100ml 1Graduated cylinder 200ml 1

Description: Measures beer and distillate volumetrically or original gravity.

Beer 5

ASBC Method: Real Extract

Preparations: Beer 4

Method: Beer 5 B

Materials and Equipment QuantityDigital density meter 1Syringes 1

Description: This method finds the real extract using the distillate left from the alcohol determination.

Beer 6

ASBC Method: Calculated values

Preparation: none

Method: Beer 6

None just formulas for calculations based on specific gravity and real extract values.

Description: This test method calculates the extract origin of wort, the Real degree of fermentation, the apparent degree of fermentation, and carbohydrate content using formulas and results from previous test methods.

Beer 8

ASBC Method: Total acidity

Preparation: Beer 1

Method: Beer 8A

Materials and equipment QuantityBuffers pH of 4.0,7.0 and 10.0 1NaOH 1Commercial pH meter digital 1Beaker 100ml 1Stir plate and stir bar 1Buret 25-50ml 1Pipette 50ml 1

Description: Measured by amount of NaOH used to neutralize the acid with a beer to a pH of 8.2.

Beer 9

ASBC method: pH (Hydrogen Ion concentration)

Preparation: Beer 1

Method: Beer 9

Materials and equipment QuantityBuffer, 4.0 7.0 pH 1pH meter 1Electrodes, glass and or reference combination

1

Thermometer 1Beakers 100ml 2

Description: reports the pH of beer.

Beer 10

ASBC method: color

Preparation: Beer 1A

Method: beer 10A

Materials and equipment QuantityReagent water 18M resistivity or greater 1LPrecision spectrophotometer 1Cuvettes 10mm 3

Description: Color is experimentally obtained by using a spectrophotometer to determine the absorbance or transmittance of the sample.

Beer 11

ASBC Method: Protein

Preparation: Beer 1

Method: Beer 11A

Materials and equipment QuantityReagent water (18M resistivity) CAS No. 7789-20-0

1

Sulfuric Acid 0.1M, and 0.05M CAS No. 7664-93-9

2

Sodium hydroxide 0.1M CAS 1310-73-2Titanium dioxide Powder Cas No. 13463-67-7 1Potassium sulfate CAS No. 7778-80-5 1Copper sulfate pentahydrate Cas No. 7758-99-8

1

2,000ml glass flask with stopper 1

Kjeldahl digestion rack 1Kjeldahl distillation unit with bulb traps and vertical condensers

1

Kjeldahl flasks 500 or 800ml 1Erlenmeyer flasks 500ml 1Pipette 25ml 1Burets 25ml 1Balance up to 500mg 1Zinc granules CAS 7440-66-6 1Methyl red indicator CAS No. 7440-66-6 1Sucrose Cas No. 57-50-1 1

Description: determines protein content for beer which is partially responsible for cloudiness and foam stability and bitterness.

Beer 12

ASBC Method: Reducing Sugar

Preparation: Beer 1

Method: Beer 12B

Materials and equipment QuantityCopper sulfate 34.639gAlkaline tartrate solution 173gAsbestos Sucrose 9.5mgWater 2000mlNaOH 50mlMethylene blue solution 1% in waterBuret 50ml 1Erlenmeyer flask 400ml 1Tripod with wire gauze 1Transfer pipet 25ml 1Volumetric flask 100ml 1

Description: reduces sugar in the beer volumetrically by the Lane Eynon method.

Beer 13

ASBC Method: Dissolved carbon dioxide

Preparation: None

Method: Beer 13C

Materials and equipment QuantityOctyl alcohol 25mlSyringe with graduated cap 1Parafilm 1Barometer in lb/in2 1

Description: Measures the dissolved carbon dioxide in beer containing up to 1.6 volume of carbon dioxide.

Beer 14

ASBC Method: Percent ash determination

Preparation: Beer 1

Method: Beer 14

Materials and equipment QuantityEvaporating dish 100ml porcelain 1Hot plate or drying oven 1Muffle furnace 1Transfer pipette 50ml 1Analytical balance 1Calculator 1

Description: Measurement of inorganic salts in the beer prior to incineration.

Beer 16

ASBC Method: End Fermentation

Preparation: Beer 3 or Beer 5

Method: Beer 16

Materials and equipment QuantityCompressed brewers’ yeast 1gFermentation flasks (Erlenmeyer 500/1000ml)

1

Fermentation seals 1Vacuum apparatus 1Buchner funnel 1Filter paper sized for lab equipment 1Graduated cylinder 250ml 1

Rotary shaker or stir plate 1Description: Allows monitoring of fermentation on a daily basis when testing new products or dealing with unknown final gravity.

Beer 18

ASBC method: Iron

Preparation: Beer 1A

Method: Beer 18B

Materials and equipment QuantityReagent Water (18MΩ resistivity) 100mlIron standard solution 1000mg/L 100mlAtomic absorption spectrophotometer 1Volumetric flask 100ml 5Pipettes 1ml graduated 5Pipettes 10ml volumetric 5

Description: determination of iron in beer using atomic absorption spectrophotometer.

Beer 20

ASBC Method: Calcium

Preparation: Beer 1

Method: Beer 20 C

Materials and equipment QuantityCalcium standard solution 1000mg/L 125mlHydrochloric acid concentrated 125mlLanthanum oxide 30mgReagent water 500mlAtomic absorption spectrophotometer 1Erlenmeyer flask 1000ml 1Volumetric flask and stopper 100ml 1Volumetric flask and stopper 500ml 1Pipettes serological 10ml 1Pipettes Volumetric 10 and 20ml 2Balance accurate to 0.001g 1

Description: Monitoring how much calcium is in the beer for product consistency.

Beer 21

ASBC Method: total sulfur dioxide

Preparation: Beer 1

Method: Beer 21A

Materials and equipment QuantityColor reagent 100mgFormaldehyde solution 5mlMercury Stabilizing solution 1LSodium hydroxide 0.1N 100mlSulfuric acid 0.1N 100mlStarch indicator 1%n-Hexyl alcoholSodium bisulfiteIodine solution 0.05NIodine solution 0.1NHydrochloric acid 35-38%Sodium thiosulfate 0.1NGraduated cylinder 10ml 1Erlenmeyer flasks with stopper 250 ml 1Volumetric flask stoppered 50ml, 100ml, 500ml

3

Pipets transfer volumetric 1, 2, 3, 4, 5, 10, 25ml

7

Pipets transfer graduated Mohr type 1, 5, 10ml

3

Burets 50mlWater bath 1Photoelectric photometer, colorimeter, or spectrophotometer

1

Description: determines total sulfur content using the p-rosaniline method.

Beer 22

ASBC Method: foam collapse rate

Preparation: beer at room temperature

Method: beer 22A

Materials and equipment QuantityIsopropyl alcohol 2ml

Special foam funnel 1Watch glass min 100mm diameter 1Graduates 100 and 25ml 2Pipet 2ml 1stopwatch 1Water bath 1

Description: Measures the rate of foam collapse.

Beer 23

ASBC Method: Beer bitterness

Preparation: Beer 1A

Method: Beer 23F

Materials and equipment Quantity2,2,4Trimethylpentane spectrophotometric grade

1

Methanol absorbance of less than 0.04 at 260nm in a 1 cm cell

1

Hydrochloric acid 3N, 4N, 6N 3Sodium hydroxide 1.5N 1Acid methanol can make 1Alkaline methanol can make 1Mechanical shaker-platform or wrist action shaker

1

Precision spectrophotometer 1Quartz cuvette 1Centrifuge tubes 50ml with Teflon lines screw caps

1

Separatory funnels 125ml 1Volumetric flasks 25ml 1Plastic transfer pipettes 1Volumetric flask 10ml 1Volumetric pipettes 5, 10, 15ml 3Graduated cylinder 10ml 1Beaker 250, 500ml 2Glass stirring rod 1

Description: measures bitterness units with IAA.

Beer 25

ASBC Methods: Diacetyl

Preparation: decarbonate beer

Method: Beer 25B

Materials and equipment QuantityΑ-Naphthol solution 1KOH-creatine solution 1Diacetyl stock solution 1Diacetyl working solution 1Colorimeter or spectrophotometer 1Distillation equipment-Boiling flask 500ml 1Distilling tube 1condenser 1Curved tapered tube adapter 1Receiving container, 50ml flask 1Volumetric flasks 10ml 1Graduated cylinders 50ml 1Heating mantle 1

Description: method determines the diacetyl content in mg/L.

Beer 26

ASBC Method: Formazin turbidity standards

Preparation: none

Method: Beer 26

Materials and equipment QuantityHydrazine sulfate CAS No. 10034-93-2 1Formin CAS No. 100-97-0 1Reagent water 18MΩ resistivity 1Erlenmeyer flask 125ml 1Volumetric flasks 100ml 1Pipettes transfer 25ml graduated by 0.1ml 1Balance accurate to 0.001g 1Nephelometer (optional) 1

Description: cloudiness or turbidity of beer can occur from many different things including storage or packaging and is measured in turbidity units.

Beer 27

ASBC method: physical stability

Preparation: beer 26

Method: beer 27A

Materials and equipment QuantityFormazin turbidity working standards 1000mlClark turbidimeter mode T1 1Red Plexiglas sheet 0.25in thick, 1 sq. foot 1Constant temperature bath 0degrees C 1Ice water bath containing a few drops of wetting agent

1

Flint glass bottles 1Description: method measures total haze after chilling

Beer 29

ASBC method: Lower boiling volatiles in beer or ale

Preparation: none

Method: Beer 29

Materials and equipment QuantityEthyl acetatei-Amyl acetaten-Propyl alcoholi-Butyl alcoholi-Amyl alcoholn-Butyl alcoholEthyl alcohol 95%Ethyl alcohol 16% aqueousInternal standard solutionCalibration solution A, and BSilanized glass woolFilter paperPipets 0.1-, 1.0-, 2.0-, 4.0mlVolumetric flasks 25-, 100mlGas chromatography with flame ionization detectionColumn 15’x0.125” stainless steel packedSyringe 25µL

Description: measures the levels of volatiles using direct injection to a sample with gas chromatography and comparison with a known standard.

Beer 30

ASBC Method: Testing for taste difference between two beers

Preparation: none

Method: Beer 30

Materials and equipment QuantityTesting beer 8ozControl beer 16ozRuby glasses 3

Description: sample beer is compared with a control beer through a taste panel.

Beer 31

ASBC Method: Free Amino Nitrogen

Preparation: Beer 1A Wort 12

Method: Beer 31

No materials used in procedures

Description: calculate the free amino nitrogen using values obtained from beer 1A and Wort 12

Beer 32

ASBC Method: Viscosity

Preparation: Beer 1A, Wort 13

Method: Beer 32

No materials used in procedure.

Description: calculate the viscosity based on values obtained from beer 1A and wort 13

Beer 33

ASBC Method: Caloric content

Preparation: Beer 2, Beer 4, Beer 5, Beer 14

Method: Beer 33

No materials used in procedure.

Description: calculate caloric content based on results from Beer 2, Beer 4, Beer 5, Beer14.

Beer 34

ASBC Method: Dissolved oxygen

Preparation: Rid of CO2 by CO2 hose insertion

Method: beer 34

Materials and equipment QuantityIndigo carmine dye solution 200mlPotassium hydroxide solution 100mlSulfuric acid 5N 100mlPotassium permanganate 0.1N 50mlPotassium permanganate 0.01N 50mlAlkaline dye solution 10mlStandard dye solution 200mlSpectrophotometer or calorimeter 1Test tubes 3Rubber self sealing insert cap 3Luer-lock syringe 10ml 3Spring loaded syringe valve attached to 6” length of 0.25” diameter rubber tubing

1

Hypodermic needles No. 21 and No.18 2Water bath 1Buret 25ml 1

Description: this method determines the colorimetric determination of dissolved oxygen in beer by the reaction of oxygen with reduced indigo carmine.

Beer 35

ASBC Method: Total phenols

Preparation: degas Beer1A

Method: beer 35

Materials and equipment Quantity

Carboxymethylcellulose (CMC) sodium salt (low viscosity), CAS 9004-32-4.

1

Ethylenediamine tetraacetic acid (EDTA), CAS6381-92-6.

1

Ferric ammonium citrate, CAS 1185-57-5. 1Ammonium hydroxide (28–30% as NH3), CAS1336-21-6.

1

Reagent water(18 MΩ resistivity or greater). 1Spectrophotometer 1Cuvettes 10mm 1Beaker 1L, 50ml 2Erlenmeyer flask 50ml with stopper 1Magnetic stir plate 1Magnetic stir bar 1Volumetric flasks 25ml, 1000ml 2Graduated cylinders 25ml wide neck with glass stoppers

2

Pipettes 10ml, 8ml, 0.5ml 3Centrifuge 1

Description: In this method, the polyphenols are reacted with ferric iron in alkaline solution, and the red color produced is measured.

Beer 36

ASBC Method: Sodium by atomic absorption spectrophotometry

Preparation: Beer 1A

Method: Beer 36

Materials and equipment QuantitySodium stock solution CAS no. 7440-23-5 10mlCesium stock solution CAS No. 7440-46-2 10mlReagent water 18 MΩ resistivity or greater 100mlAtomic absorption spectrophotometer 1Volumetric flask stoppered 100ml, 1000ml 2Pipettes 1, 3, 5, 7, 9, 10ml 6

Description: using atomic absorption spectrophotometer determines the concentration of sodium.

Beer 37

ASBC method: Potassium by atomic absorption spectrophotometry

Preparation: Beer 1A

Method: Beer 37

Materials and equipment QuantityPotassium stock solution CAS no. 7447-40-7 10mlCesium stock solution CAS No. 7440-46-2 10mlReagent water 18 MΩ resistivity or greater 100mlAtomic absorption spectrophotometer 1Volumetric flask stoppered 100ml, 1000ml 2Pipettes 1, 3, 5, 7, 9, 10ml 6

Description: using atomic absorption spectrophotometer determines the concentration of potassium.

Beer 38

ASBC Method: Magnesium and Calcium

Preparation: Beer 1A

Method: Beer 38A

Materials and equipment QuantityHydrochloric acid 1Magnesium stock solution 1Lanthanum solution 1Atomic absorption spectrophotometer 1Magnesium hollow cathode lamp 1Volumetric flasks 1Pipets 1

Description: determines the magnesium and Calcium concentrations.

Beer 39

ASBC Method: Chloride

Preparation: Beer 1A

Method: Beer 39B

Materials and equipment QuantityReagent water 18MΩ or greater 125mlSilver nitrate CAS No. 7761-88-8 1

Sodium Chloride Cas No. 7647-14-5 1Conductivity meter and cell with platinum electrodes

1

Beaker 1Volumetric flask 1L 1Pipettes 3ml, 25ml 2Buret with 0.25ml increments 1Stir plate and bar 1

Description: Determines the chloride concentration by conductivity meter titration.

Beer 40

ASBC Method: N-Nitrosamines

Preparation: Decarbonate

Method: Beer 40A

Materials and equipment QuantityBarium hydroxide 1Boileezers CAT No. B-365 1Sodium carbonate 1Dichloromethane 1Sodium sulfate 1Dry nitrogen 1Standards N-nitrosodimethylamine and N-nitroso-dipropylamine

2

Internal standard solution 1Ethanol 1Distilled water 1Volumetric pipets assorted in size 1Distillation flask 1L 1Heating mantles for 1L flask 1Variable transformers for heating mantles 1Separatory funnels 250ml 1Fritted glass funnel 60ml 1Evaporative concentrator 1Water bath 60 degrees 1Syringe 10uL for gas chromatography 1Gas chromatograph interfaced with TEA model 502 analyzer

1

Gas chromatographic column capable of baseline separation

1

Flasks assorted Erlenmeyer and volumetric 1Description: Measures N-Nitrosamines by distillation. Warning Nitrosamines are a known carcinogenic.

Beer 41

ASBC Method: Total Carbohydrate

Preparation: Beer 1A

Method: beer 41A

Materials and equipment QuantityPhenol solution 5% w/v in water 100mLSulfuric acid concetrted reagent grade 100mlDextrose calibration standard 1LPipette 5ml fixed volume 1Flasks 100ml, 1L volumetric 2spectrophotometer 1Spectrophotometer cells 1Test tubes 5Pipettes volumetric 1ml, 2ml 2Vortex mixer 1

Description: Dextrose and phenol, in the presence of sulfuric acid, react further to produce a colored complex that is measured spectrophotometrically

Beer 42

ASBC Method: Aluminum by Graphite Furnace Atomic Absorption Spectrophotometry

Preparation: degas by filtration

Method: Beer 42

Materials and equipment QuantityAluminum standard 1000mg/1L 100mlNitric acid CAS No. 7697-37-2 100mlReagent water 200mlMagnesium nitrate matrix modifier 1Magnesium nitrate CS No. 13446-18-9 10mlGraphite furnace atomic absorption spectrophotometer.

1

Aluminum hollow cathode lamp 1

Flasks, 10-, 100-, and 1,000-mL volumetric 2Pipettes, 1-, 2-, 6-, and 10-mL volumetric. 4Filter paper, Whatman No. 4 or equivalent. 1

Description: The aluminum content of beer is determined by atomizing a sample in a graphite furnace and comparing the absorbance of the resulting ground-state atom.

Beer 43

ASBC Method: Anions (Chloride, Phosphate, and Sulfate) by Ion Chromatography

Preparation: degassing

Method: Beer 43

Materials and equipment QuantityDeionized water 18MA resistivity 1Sodium chloride 1Sodium phosphate 1Sodium sulphate 1Sodium carbonate 1Sodium bicarbonate 1Ion chromatograph 1Electronic integrator or data system 1Volumetric flask 50 or 100ml 1Balance accurate to 0.1mg 1Ultrasonic bath 1Chromatographic guard column AG4A 1Chromatographic analytical column AS4A 1Filter assembly 1

Description: This method utilizes ion chromatography with suppressed conductivity detection to separate and quantitate chloride, phosphate, and sulfate in beer.

Beer 44

ASBC Method: Dimethyl Sulfide by Chemiluminescence Detection

Preparation: none

Method: Beer 44

Materials and equipment QuantityReagent water 1Antifoam B, aqueous/silicone emulsion 1

Ethyl alcohol CAS No. 64-17-5 1Dimethyl sulfide CAS No. 75-18-3 1Ethyl Methyl sulfide CAS No. 624-89-5 1Bottle piercing device 1Syringes 50, 100, 250ml 1Volumetric flasks 100ml 1Gas chromatograph 1Chemiluminescence detector 1Parafilm 1Ice Bath 1

Description: This gas-chromatographic headspace method employs chemiluminescence for the detection of DMS in finished beer.

Beer 45

ASBC Method: Elemental Analysis by Inductively Coupled Plasma-Atomic Emission Spectroscopy

Preparation: Beer 1D

Method: Beer 45

Materials and equipment QuantityNitric acid 70% 20mlEthyl Alcohol 200proof 200mlSodium standard 10,000mg/L 1Potassium standard 10,000mg/L 1Magnesium standard 10,000mg/L 1Calcium standard 10,000mg/L 1Yttrium standard 10,000mg/L 1Gallium standard 10,000mg/L 1Copper standard 1,000mg/L 1Iron standard 1,000mg/L 1Deionized water 18MΩ resistivity or greater 1Calibration solution 1Internal standard 1Blank solution 1ICP-AES (ex. Perkin Elmer Optima or Varian Vista with baffled cyclonic spray chamber or equivalent.)

1

Volumetric flasks 10ml 1Volumetric flasks 100ml 3Pipettes capable of delivering 50uL, 200uL, 1ml, 5ml, 7ml, 10ml

1

Description: The instrument measures the elemental concentrations based on the emission of light for each element.

Beer 46

ASBC Method: Measurement of Oxidative Resistance in Beer by Electron Paramagnetic Resonance

Preparation: Thaw overnight and degas with vortex mixer.

Method: Beer 46

Materials and equipment Quantity Α-Phenyl-t-butylnitrone CAS No. 3376-24-7 14-Hydroxy-2,2,6,6-tetreamethylpiperidine 1Ethanol absolute CAS No. 64-17-5 1Reagent water CAS No. 64-17-5 1EPR Spectrometer 9-10GHz 1Automatic sample changer system 1Heating bath or heating block 1Sonicator 1Sample tubes 3Pipettes 5uL, 100uL, 1000uL, 5ml 4Vortex mixer 1Analytical balance 1Thermometer accurate at 60 C 1Volumetric flask 10ml 1

Description: measures the beers oxidative resistance.

Beer 48

ASBC Method: Headspace Gas Chromatography–Flame Ionization Detection Analysis of Beer Volatiles

Preparation: cool in an ice bath

Method: Beer 48

Materials and equipment QuantityReagent water 1Ethanol 200 proof CAS 64-17-5 1n-Butanol CAS 71-36-3 1Acetaldehyde CAS 75-07-0 1

Ethyl acetate CAS 141-78-6 1Isoamyl acetate CAS 123-92-2 1Isoamyl alcohol CAS 123-51-3 1Static headspace autosampler 1Gas chromatograph 1Column- Stabilwax, 60 m × 0.32 mm ID × 1 μm film thickness

1

Vortex mixer 1Autosampler vials 5Crimp top septa aluminum ring with Teflon-faced septa.

1

Syringes air tight, 100, 250, 500uL 3Glass pipettes class A volumetric 0.5, 1, 5, 10, 20

5

Glass pipettes serological 5ml 1Micropipette 100uL 1Volumetric flasks class a 50, 100, 1000ml 3

Description: This method can be used to identify and quantify the generation of volatile organic compounds produced through fermentation.

Beer 49

ASBC method: Determination of Gluten Using the R5 Competitive ELISA Method.

Preparation: decarbonate by shaking in an Erlenmeyer flask or rotary shaker.

Method: beer 49

Materials and equipment QuantityDistilled water 1Fish gelatin CAS 9000-70-8 1Ethanol >99.5% CAS 64-19-5 1Sodium hydroxide 1N CAS 1310-73-2 1Sample dilution buffer 1Enzyme conjugate concentrate 1Washing buffer concentrate 1Standard solutions 1Substrate/chromogen 1Stop solution 1Graduated cylinder 500ml 1Erlenmyer flasks 50ml 1Beakers 50, 100, 500, 1000 1Pipettes 10-100 and 100-1000uL 6

Centrifuge tubes .3 and 15ml 596-well spectrophotometer or strip reader capable of reading at 450nm wavelength

1

Ridascreen gliadin competitive kit 7021, R-biopharm

1

Description: detect gluten in beer and any residual hydrolyzed prolamin so that beer can confidently be labelled as gluten free.

Beer 50

ASBC Method: Foam stability in Nibem

Preparation: none

Method: Beer 50

Materials and equipment QuantityCarbon dioxide CAS No. 630-08-0 1Sodium hydroxide 0.25M CAS No. 1310-73-2 1Reagent water distilled and deionized 1Tap water 1Ethanol 97% v/V CAS No. 64-17-5 1Water bath 1NIBEM-T or NIBEM-TPH tester 1Inpack 2000 sampling device 1NIBEM glass 60mm inside diameter and 120mm height

1

Small glass or beaker 1Empty beer bottle 1CO2 regulator capable of holding pressure at 2bar

1

Hygrometer digital NIST certified 1Barometer digital NIST certified 1Safety net for covering bottles 1

Description: measures foam stability, the amount of time it takes for foam to collapse a specified distance.

Inventory of required materials equipment and reagents

Material Quantity2,2,4Trimethylpentane spectrophotometric grade

1

4-Hydroxy-2,2,6,6-tetreamethylpiperidine 195% ethyl alcohol 1Acetaldehyde CAS 75-07-0 1Acid methanol can make 1Alcohol 70% ethyl or isopropyl 1ml Alkaline dye solution 10mlAlkaline methanol can make 1Alkaline tartrate solution 173gAluminum foil 1Aluminum hollow cathode lamp 1Aluminum standard 1000mg/1L 100mlAmmonium hydroxide (28–30% as NH3), CAS1336-21-6.

1

Analytical balance 1Antifoam B, aqueous/silicone emulsion 1Asbestos 1Atomic absorption spectrophotometer 1Automatic beer analyzer 1Automatic sample changer system 1Autosampler vials 5Balance accurate to 0.001g and up to 500g 1Barium hydroxide 1Barometer digital NIST certified 1Barometer in lb/in2 1Beaker 50ml 3Beaker 100ml 2Beaker 250ml 2beakers 500ml 1Beakers 1000 1Boileezers CAT No. B-365 1Boiling flask 500ml 1Bottle piercing device 1Buchner funnel 1Buffers pH of 4.0,7.0 and 10.0 1Buret 25ml 1Buret 50ml 1Butane torch 1Calcium standard 10,000mg/L 125mlCalcium standard solution 1000mg/L 1Calculator 1Calibration solution 1Calibration solution A, and B 1

Carbon dioxide CAS No. 630-08-0 1Carboxymethylcellulose (CMC) sodium salt (low viscosity), CAS 9004-32-4.

1

Centrifuge 1Centrifuge tubes .3 and 15ml 1Centrifuge tubes 50ml with Teflon lines screw caps

5

Cesium stock solution CAS No. 7440-46-2 10mlChemiluminescence detector 1Chromatographic analytical column AS4A 1Chromatographic guard column AG4A 1Clark turbidimeter mode T1 1CO2 regulator capable of holding pressure at 2bar

1

Color reagent 1Column 15’x0.125” stainless steel packed 1Column- Stabilwax, 60 m × 0.32 mm ID × 1 μm film thickness

1

Commercial pH meter digital 1Compressed brewers’ yeast 1Conditioning solution 1Conductivity meter and cell with platinum electrodes

1

Constant temperature bath 0degrees C 1Control beer 1Copper standard 1,000mg/L 34.639gCopper sulfate 1Copper sulfate pentahydrate Cas No. 7758-99-8 1Crimp top septa aluminum ring with Teflon-faced septa.

1

Curved tapered tube adapter 1Cuvettes 10mm 1Deionized water 18MΩ resistivity or greater 1Detergent as needed for apparatus 1Dextrose calibration standard 1Diacetyl stock solution 1Diacetyl working solution 1Dichloromethane 1Digital density meter 1Dimethyl sulfide CAS No. 75-18-3 1Distillation equipment- 1Distillation flask 1L 1

Distilled water 1Distilling tube 1Dry nitrogen 1Electrodes, glass and or reference combination 1Electronic integrator or data system 1Empty beer bottle 1Enzyme conjugate concentrate 1EPR Spectrometer 9-10GHz 1Erlenmeyer flask 50ml 1Erlenmeyer flask 125ml 1Erlenmeyer flask 400ml with stopper 3Erlenmeyer flasks 500ml 1Erlenmeyer flasks with stopper 250 ml 1Ethanol >99.5% CAS 64-19-5 1Ethanol 200 proof CAS 64-17-5 1Ethanol 97% v/V CAS No. 64-17-5 1Ethanol absolute CAS No. 64-17-5 1Ethyl acetate CAS 141-78-6 1Ethyl alcohol 16% aqueous 200mlEthyl Methyl sulfide CAS No. 624-89-5 1Ethylenediamine tetraacetic acid (EDTA), CAS6381-92-6.

1

Evaporating dish 100ml porcelain 1Evaporative concentrator 1Fermentation flasks (Erlenmeyer 1000ml) 1Fermentation seals 1Ferric ammonium citrate, CAS 1185-57-5. 1Filter assembly 1Filter paper 1Filter paper sized for lab equipment 1Filter paper, Whatman No. 4 or equivalent. 1Fish gelatin CAS 9000-70-8 1Flint glass bottles 1Formaldehyde solution 1000mlFormazin turbidity working standards 1Formin CAS No. 100-97-0 1Fritted glass funnel 60ml 1Gallium standard 10,000mg/L 1Gas chromatograph 1Gas chromatograph interfaced with TEA model 502 analyzer

1

Gas chromatographic column capable of baseline separation

Gas chromatography with flame ionization detection

1

Glass stirring rod 1Graduated cylinder 100ml 1Graduated cylinder 10ml 1Graduated cylinder 200ml 1Graduated cylinder 250ml 1Graduated cylinder 500ml 2Graduated cylinders 25ml wide neck with glass stoppers

1

Graduated cylinders 50ml 2Heating bath or heating block 1Heating mantles for 1L flask 1Hot plate or drying oven 1Hydrazine sulfate CAS No. 10034-93-2 1Hydrochloric acid 35-38% 1Hydrochloric acid 3N, 4N, 6N 125mlHydrochloric acid concentrated 1Hydrometer cylinder glass or stainless steel 2 (1 for

wort and 1 for beer)

Hygrometer digital NIST certified 2Hypodermic needles No. 21 and No.18 1Hypodermic syringes or autosampler 1i-Amyl acetate 1i-Amyl alcohol 1i-Butyl alcohol 1Ice water bath containing a few drops of wetting agent

1

ICP-AES (ex. Perkin Elmer Optima or Varian Vista with baffled cyclonic spray chamber or equivalent.)

1

Indigo carmine dye solution 1Inpack 2000 sampling device 1Internal standard solution 1Iodine solution 0.05N 1Iodine solution 0.1N 1Ion chromatograph 1Iron standard 1,000mg/L 100mlIsoamyl acetate CAS 123-92-2 1Isoamyl alcohol CAS 123-51-3 2ml

Isopropyl alcohol 1Kjeldahl digestion rack 1Kjeldahl distillation unit with bulb traps and vertical condensers

1

Kjeldahl flasks 500 or 800ml 1KOH-creatine solution 30mgLanthanum oxide 1Lanthanum solution 1Luer-lock syringe 10ml 1Magnesium hollow cathode lamp 1Magnesium nitrate CS No. 13446-18-9 1Magnesium nitrate matrix modifier 1Magnesium standard 10,000mg/L 1Magnesium stock solution 1Magnetic stir bar 1Magnetic stir plate 1Mechanical shaker-platform or wrist action shaker

1

Mercury Stabilizing solution 1Methanol absorbance of less than 0.04 at 260nm in a 1 cm cell

1

Methyl red indicator CAS No. 7440-66-6 1Methylene blue solution 1% in water 1Micropipette 100uL 1Muffle furnace 1n-Butanol CAS 71-36-3 1n-Butyl alcohol 1Nephelometer (optional) 1n-Hexyl alcohol 1NIBEM glass 60mm inside diameter and 120mm height

1

NIBEM-T or NIBEM-TPH tester 1Nitric acid 70% 100mlNitric acid CAS No. 7697-37-2 100mlN-nitroso-dipropylamine 100mln-Propyl alcohol 25mlOctyl alcohol 1Parafilm 1Phenol solution 5% w/v in water 1Pipet tips 10-100µl 5Pipets 0.1-, 1.0-, 2.0-, 4.0ml 1Pipets transfer graduated Mohr type 10ml 1

Pipets transfer graduated Mohr type 1ml 1Pipets transfer graduated Mohr type 5ml 1Pipets transfer volumetric 10ml 1Pipets transfer volumetric 1ml 1Pipets transfer volumetric 2ml 1Pipets transfer volumetric 3ml 1Pipets transfer volumetric 4ml 1Pipets transfer volumetric 5ml 1Pipette 25ml 1Pipette 50ml 1Pipette 5ml fixed volume 1Pipettes 10-100 and 100-1000uL 3Pipettes 0.5ml 4Pipettes 5uL, 100uL, 1000uL, 5ml 1Pipettes serological 10ml 1Pipettes transfer 25ml graduated by 0.1ml 2Potassium hydroxide solution 50mlPotassium permanganate 0.01N 50mlPotassium permanganate 0.1N 1Potassium standard 10,000mg/L 10mlPotassium stock solution CAS no. 7447-40-7 1Potassium sulfate CAS No. 7778-80-5 1Precision spectrophotometer 1Quartz cuvette 2Reagent water 18M resistivity or greater CAS No. 7789-20-0

1

Receiving container, 50ml flask 1Red Plexiglas sheet 0.25in thick, 1 sq. foot 1Ridascreen gliadin competitive kit 7021, R-biopharm

1

Rotary shaker lab line model 3518 or equivalent 3Rubber self sealing insert cap 3Ruby glasses 4Safety net for covering bottles 1Sample dilution buffer 3Sample tubes 1Separatory funnels 125ml 1Separatory funnels 250ml 1Silanized glass wool 1Silver nitrate CAS No. 7761-88-8 1Small glass or beaker 1Sodium bicarbonate 1

Sodium bisulfite 1Sodium carbonate 1Sodium Chloride Cas No. 7647-14-5 1Sodium hydroxide 0.1M CAS 1310-73-2 100mlSodium hydroxide 0.25M CAS No. 1310-73-2 1Sodium hydroxide 1.5N 1Sodium hydroxide 1N CAS 1310-73-2 1Sodium phosphate 1Sodium standard 10,000mg/L 10mlSodium stock solution CAS no. 7440-23-5 1Sodium sulfate 1Sodium thiosulfate 0.1N 1Sonicator 1Special foam funnel 1Spectrophotometer cells 1Spring loaded syringe valve attached to 6” length of 0.25” diameter rubber tubing

1

Standard dye solution 1Standard solutions 2Standards N-nitrosodimethylamine and 1Starch indicator 1% 1Static headspace autosampler 1Sterile pipe cleaners 2Sterile sample flasks 1Sterile Sampling needles 1Stir plate and stir bar 1Stop solution 1stopwatch 1Substrate/chromogen 1Sucrose Cas No. 57-50-1 1LSulfuric Acid 0.1M, and 0.05M CAS No. 7664-93-9 100mlSulfuric acid 0.1N 100mlSulfuric acid 5N 100mlSulfuric acid concetrted reagent grade 1Syringe 10uL for gas chromatography 1Syringe 25µL 1Syringe with graduated cap 1Syringes 50, 100, 250ml 3Syringes air tight, 100, 250, 500uL 1Tap water 3Test tubes 5Thermometer 1

Titanium dioxide Powder Cas No. 13463-67-7 1Transfer pipette 50ml 2Tributyl Phosphate (TBP) Fisher B-404-500 1Tripod with wire gauze 1Ultrasonic bath 1Vacuum apparatus 1Variable transformers for heating mantles 1Volumetric flask 10ml 5Volumetric flask 25ml and stopper 1Volumetric flask 50ml 1Volumetric flask 100ml 1Volumetric flask and stopper 500ml 2Volumetric flask stoppered 1000ml 2Volumetric flask 2,000mk 3Vortex mixer 1Washing buffer concentrate 1Watch glass min 100mm diameter 1Water bath 1Yttrium standard 10,000mg/L 1Zinc granules CAS 7440-66-6 1Α-Naphthol solution 1Α-Phenyl-t-butylnitrone CAS No. 3376-24-7 1

List of needed items to purchase

Materials and equipment Quantity Price per unit in USD

Total in USD

2,000ml glass flask with stopper 1 23.95 23.95 Aluminum foil 1 roll 9.99 9.99Aluminum hollow cathode lamp 1 225.00 225.00Automatic beer analyzer 1 - -Automatic sample changer system 1 - -Autosampler vials 5 - -Balance accurate to 0.001g and up to 500g 1 152.00 152.00Barometer digital NIST certified 1 155.54 155.54Beaker 50ml 3 14.95 14.95Beaker 100ml 5 6.94 34.70Beaker 250ml 4 9.34 37.36beakers 600ml 4 11.39 45.56Beakers 1000 2 14.25 28.50Boiling flask 500ml 1 31.80 31.80

Bottle piercing device 1 775.00 775.00Buchner funnel 1 25.57 25.57Buret 25ml 1 59.95 59.95Buret 50ml 1 201.00 201.00Butane torch 1 44.98 44.98Calculator 1 28.77 28.77Centrifuge 1 312.28 312.28Centrifuge tubes .3 and 15ml 10 13.80 138.00Centrifuge tubes 50ml with Teflon lines screw caps 10 79.00 790.00Chemiluminescence detector 1 - -Chromatographic analytical column AS4A 1Chromatographic guard column AG4A 1CO2 regulator capable of holding pressure at 2bar 1 45.19 45.19Column 15’x0.125” stainless steel packed 1 238.00 238.00Column- Stabilwax, 60 m × 0.32 mm ID × 1 μm film thickness

1 543.00 543.00

Commercial pH meter digital 1 67.59 67.59Conductivity meter and cell with platinum electrodes 1 149.00 149.00Constant temperature water bath 0degrees C 1 401.74 401.74Crimp top septa aluminum ring with Teflon-faced septa. 1 148.64 148.64Curved tapered tube adapter 1 111.61 111.61Cuvettes 10mm 1 54.60 54.60Distillation flask 1L 1 66.39 66.39Distilling tube 1 113.08 113.08Electrodes, glass and or reference combination 1 75.00 75.00Electronic integrator or data system 1Empty beer bottle 1 21.34 21.34Erlenmeyer flask 50ml 1 13.89 13.89Erlenmeyer flask 125ml 2 11.25/3 11.25Erlenmeyer flasks 500ml 2 8.34 16.70Erlenmeyer flasks with stopper 250 ml 2 11.46/3 11.46Evaporating dish 100ml porcelain 1 6.24 6.24Evaporative concentrator 1 28.17 28.17Fermentation flasks (Erlenmeyer 1000ml) 1 18.48 18.48Fermentation seals 1 6.73 6.73Filter assembly 1 259.97 259.97Filter paper, Whatman No. 4 or equivalent. 1 11.83 11.83Flint glass bottles 1 10.22 10.22Fritted glass funnel 60ml 1 87.15 87.15Glass stirring rod 1 9.00 9.00Graduated cylinder 100ml 2 6.33 12.66Graduated cylinder 10ml 1 5.99 5.99

Graduated cylinder 250ml 2 12.03 24.06Graduated cylinder 500ml 2 55.00 110.00Graduated cylinders 25ml wide neck with glass stoppers 1 19.32 19.32Graduated cylinders 50ml 2 5.88 11.76Heating bath or heating block 1 425.28 425.28Hypodermic needles No. 21 and No.18 1 25.80 25.80Hypodermic syringes or autosampler 1 7.99 7.99Luer-lock syringe 10ml 1 7.60 7.60Magnesium hollow cathode lamp 1 476.00 476.00Magnetic stir bar 1 20.95 20.95Magnetic stir plate 1 348.50 348.50Mechanical shaker-platform or wrist action shaker 1 2,368.00 2,368.00Micropipette 100uL 1 22.95 22.95Parafilm 1 100.70 100.70Pipette tips 10-100µl 1 166.60 166.60Pipettes 0.1-, 1.0-, 2.0-, 4.0ml 1 19.80 19.80Pipettes transfer graduated Mohr type 10ml 1 6.75 6.75Pipettes transfer graduated Mohr type 1ml 1 6.75 6.75Pipettes transfer graduated Mohr type 5ml 1 6.75 6.75Pipettes transfer volumetric 1-10ml 1 55.00 55.00Pipette 25ml 1 19.37 19.37Pipette 50ml 1 12.99 12.99Pipettes 100-1000uL 1 112.50 112.50Quartz cuvette 1 146.00 146.00Receiving container, 50ml flask 1 8.99 8.99Red Plexiglas sheet 0.25in thick, 1 sq. foot 1 13.86 13.86Rubber self sealing insert cap 1 15.50 15.50Ruby glasses 1 29.95 29.95Safety net for covering bottles 1 4.49 4.49Sample tubes 1 116.70 116.70Separatory funnels 125ml 1 151.00 151.00Separatory funnels 250ml 1 180.00 180.00Silanized glass wool 1 51.00 51.00Sonicator 1 1,665.00 1,665.00Spring loaded syringe valve attached to 6” length of 0.25” diameter rubber tubing

1 469.41 469.41

Sterile pipe cleaners 1 19.37/50

19.37

Sterile sample flasks 1 151.40 151.40Sterile Sampling needles 1 18.93 18.93stopwatch 1 16.99 16.99Syringe 10uL for gas chromatography 1 42.00 42.00

Syringe 25µL 1 48.00 48.00Syringe with graduated cap 1 8.99 8.99Syringes 50, 100, 250ml 3 12.99 38.97Syringes air tight, 100, 250, 500uL 3 55.50 166.50Test tubes 1 38.89 38.89Thermometer 1 30.11 30.11Tripod with wire gauze 1 14.75 14.75Vacuum apparatus 1 282.00 282.00Variable transformers for heating mantles 1 723.70 723.70Volumetric flask 10ml 2 4.50 9.00Volumetric flask 25ml and stopper 2 27.00 54.00Volumetric flask 50ml 2 27.00 54.00Volumetric flask 100ml 2 152.00/

6152.00

Volumetric flask and stopper 500ml 2 270.00/6

270.00

Volumetric flask stoppered 1000ml 2 52.00 104.00Volumetric pipets assorted in size 1 116.80 116.80Vortex mixer 1 375.00 375.00Watch glass min 100mm diameter 1 26.00/1

226.00

Total budget for all lab equipment 10,519.80

List of references used for pricing

https://www.amazon.com/2000-Fermenter-Flask-Stopper-Airlock/dp/B00A7FUZPS

https://www.amazon.com/Reynolds-Wrap-Aluminum-Foil-Square/dp/B00I9J3Q8W/ref=sr_1_3?ie=UTF8&qid=1531805139&sr=1-3&keywords=aluminum+foil

https://buck-scientific-norwalk.myshopify.com/products/aluminum-al-hollow-cathode-lamp

https://www.amazon.com/Analytical-Balance-Laboratory-Precision-Balances/dp/B0757LRCFW

https://www.amazon.com/Griffin-Borosilicate-Graduated-Karter-Scientific/dp/B07CC27HX8

https://www.amazon.com/PYREX-Griffin-100mL-Beaker-Graduated/dp/B003TV9LQG/ref=sr_1_1?ie=UTF8&qid=1531805921&sr=8-1&keywords=pyrex+beaker+100ml

https://www.amazon.com/PYREX-Griffin-1000mL-Beaker-Graduated/dp/B003TVDGQM/ref=sr_1_3?ie=UTF8&qid=1531806192&sr=8-3&keywords=pyrex+beaker+1000ml

https://www.amazon.com/Chemglass-CG-1506-20-Single-Bottom-Standard/dp/B005WVQZYW/ref=sr_1_4?ie=UTF8&qid=1531806288&sr=8-4&keywords=boiling+flask+500ml

http://www.zahmnagel.com/shop/testing-equipment/part-11000-zahm-modified-piercing-device/

https://www.amazon.com/United-Scientific-Supplies-JBF600-Diameter/dp/B00N50MLXG/ref=sr_1_10?ie=UTF8&qid=1531806510&sr=8-10&keywords=buchner+funnel

https://www.amazon.com/dp/B00394F9PA/ref=sspa_dk_detail_0?psc=1&pd_rd_i=B00394F9PA&pf_rd_m=ATVPDKIKX0DER&pf_rd_p=1713835751726239774&pf_rd_r=8JKHCQNAEMQMM0QHYYJ6&pd_rd_wg=36LRR&pf_rd_s=desktop-dp-sims&pf_rd_t=40701&pd_rd_w=jzw7a&pf_rd_i=desktop-dp-sims&pd_rd_r=188030c6-8985-11e8-ae49-d7f1b6fa8ade

https://www.hach.com/buret-class-a-glass-50-ml/product?id=7640238638&_bt=218809546514&_bk=&_bm=b&_bn=g&gclid=EAIaIQobChMIz5Pd67il3AIVBNVkCh1y3QUmEAAYAiAAEgJwJvD_BwE

https://www.amazon.com/Texas-Instruments-TI-36X-PRO-Scientific/dp/B00EXN5VJA/ref=sr_1_4?s=industrial&ie=UTF8&qid=1531806874&sr=1-4&keywords=scientific+calculator

https://www.amazon.com/Ample-Scientific-Bench-Top-Centrifuge-550-3300rpm/dp/B004I8WSBO/ref=sr_1_4?ie=UTF8&qid=1531807099&sr=8-4&keywords=centrifuge

https://www.amazon.com/Karter-Scientific-Centrifuge-17x120mm-No-Leak/dp/B005Z4QSOS/ref=pd_sim_328_7?_encoding=UTF8&pd_rd_i=B005Z4QSOS&pd_rd_r=7d5efc1d-8986-11e8-aaf6-81458bf8879e&pd_rd_w=a0BCC&pd_rd_wg=ZNzrw&pf_rd_i=desktop-dp-sims&pf_rd_m=ATVPDKIKX0DER&pf_rd_p=2610440344683357453&pf_rd_r=PZG8RRJP41S20XAQZD67&pf_rd_s=desktop-dp-sims&pf_rd_t=40701&psc=1&refRID=PZG8RRJP41S20XAQZD67

https://www.amazon.com/Teflon-Centrifuge-Bottom-Chemistry-Labware/dp/B0757GTKGM/ref=sr_1_fkmr0_1?s=industrial&ie=UTF8&qid=1531807267&sr=1-1-fkmr0&keywords=Centrifuge+tubes+50ml+with+Teflon+lines+screw+caps

https://www.amazon.com/OneBom-Regulator-Pressure-Relief-0-3000PSI/dp/B07DC48YYL/ref=sr_1_1_sspa?ie=UTF8&qid=1531808057&sr=8-1-spons&keywords=beer+regulator+co2&psc=1

https://www.sigmaaldrich.com/catalog/product/supelco/13052u?lang=en&region=US

https://www.fishersci.com/shop/products/restek-stabilwax-capillary-columns-103/p-2330619

https://www.vernier.com/products/sensors/conductivity-probes/conpt-bta/

https://www.thomassci.com/Equipment/General-Purpose-Baths/_/Digital-General-Purpose-Water-Baths?q=Temperature%20Controlled%20Water%20Bath

https://www.thomassci.com/Laboratory-Supplies/Sampling-Serum-Bottles/_/TEFLON-FACED-GRAY-BUTYL-SEPTA?q=Aluminum%20Septa

https://www.fishersci.com/shop/products/kontes-inlet-flow-control-adapters/k1850002440

https://www.sigmaaldrich.com/catalog/product/sigma/g10?lang=en&region=US&gclid=EAIaIQobChMI_LnXhsKl3AIVi-NkCh0CmQv5EAAYASAAEgJQqPD_BwE

https://www.thomassci.com/Laboratory-Supplies/Distilling-Flasks/_/PYREX-DISTILLING-FLASKS?q=Distillation%20Flask

https://www.thomassci.com/Equipment/Water-Distillation/_/893024-TUBE-2440-CS1-CORNING?q=distilling%20tube

https://www.thomassci.com/Instruments/Electrodes-pH/_/COMBINATION-ELECTRODES?q=Combination%20Electrode

https://www.amazon.com/Kimble-Concentrator-Evaporative-Concentrators-Capacity/dp/B009NWRB4O/ref=sr_1_1?ie=UTF8&qid=1531810486&sr=8-1&keywords=evaporative+concentrator

https://www.amazon.com/EVAccess75-S-Shape-Grommet-Homebrew-Fermentation/dp/B00XDSJ25K/ref=sr_1_5?ie=UTF8&qid=1531810643&sr=8-5&keywords=fermentation+seal

https://www.sterlitech.com/all-glass-filtration-assemblies.html?wtx=GAW&gclid=EAIaIQobChMI1fCl2cel3AIVRJJ-Ch0flg9NEAAYAyAAEgKrYPD_BwE

https://www.bottlestore.com/16oz-flint-boston-round-glass-bottle-28-400-neck.html

https://www.thomassci.com/Laboratory-Supplies/Filtering-Funnels/_/PYREX-BUCHNER-FILTER-FUNNELS-WITH-FRITTED-GLASS-DISC-DESIGN?q=Fritted%20Disc%20Funnel

https://www.amazon.com/PolyScience-WB05A11B-Digital-General-Capacity/dp/B00N5ILOK4/ref=sr_1_3?s=industrial&ie=UTF8&qid=1531856024&sr=1-3&keywords=heating+bath

http://www.perkinelmer.com/product/lumina-hollow-cathode-2-lamp-mg-n3050144

https://www.hach.com/stir-bar-magnetic-arrester/product?id=7640225863&_bt=218809546514&_bk=&_bm=b&_bn=g&gclid=EAIaIQobChMI34v_l_Om3AIV3rXACh3ZCALsEAAYAiAAEgIAr_D_BwE

https://www.grainger.com/product/BENCHMARK-SCIENTIFIC-Magnetic-Stirrer-9KWD5?cm_sp=Product_Details-_-Products_Based_on_Your_Search-_-IDPPLARECS&cm_vc=IDPPLARECS

https://www.fishersci.com/us/en/products/I9C8L35Y/wrist-action-shakers.html

https://www.homesciencetools.com/product/100-ul-micropipette/

https://www.sigmaaldrich.com/catalog/product/sigma/p7793?lang=en&region=US&gclid=EAIaIQobChMIqMGBr_Wm3AIVxbfACh3AJQ_FEAAYASAAEgLxH_D_BwE

https://www.carolina.com/lab-pipets/fast-release-pipet-pump-ll/FAM_736862.pr

https://www.amazon.com/Microlit-Lab-Micropipette-Single-Channel-Autoclavable/dp/B07C11MJG9/ref=sr_1_1_sspa?ie=UTF8&qid=1531857858&sr=8-1-spons&keywords=10ml+volumetric+pipette&psc=1

https://www.coleparmer.com/p/cole-parmer-standard-single-quartz-cuvettes-for-uv-spectroscopy/63299?Ntt=Quartz+Cuvettes

https://www.lowes.com/pd/Palight-ProjectPVC-Red-Foam-PVC-Sheet-Common-12-in-x-12-in-Actual-12-in-x-12-in/1000088055?cm_mmc=SCE_PLA_ONLY-_-ToolsAndHardware-_-SosScreenGlass-_-1000088055:Palight_ProjectPVC&CAWELAID=&kpid=1000088055&CAGPSPN=pla&k_clickID=382a3eee-4bf2-4afc-9d01-3aa26444eb34&gclid=EAIaIQobChMI-_T1xvmm3AIVirrACh1UUQZZEAYYAiABEgJHH_D_BwE

https://food.r-biopharm.com/products/ridascreen-gliadin-competitive/

https://www.amazon.com/Pack-Ruby-Ounce-Drink-Glass/dp/B00OM5JVMW/ref=sr_1_13?ie=UTF8&qid=1531859344&sr=8-13&keywords=ruby+glassware

https://www.kegworks.com/protective-mesh-webbing-liquor-bottle-sleeves

https://www.sigmaaldrich.com/catalog/product/aldrich/br114715?lang=en&region=US&cm_sp=Insite-_-prodRecCold_xviews-_-prodRecCold10-7

https://www.coleparmer.com/p/pyrex-brand-6402-funnels/2003?Ntt=Lab+Funnels+Separatory

https://www.sigmaaldrich.com/catalog/product/supelco/20411?lang=en&region=US&gclid=EAIaIQobChMItqXjk_-m3AIVD5BpCh2GgQihEAAYASAAEgIsHfD_BwE

https://homogenizers.net/products/q55-sonicator?utm_medium=cpc&utm_source=googlepla&gclid=EAIaIQobChMIm8_QiICn3AIVmLjACh1iOAYyEAYYAyABEgKsHPD_BwE

http://www.teledynetekmar.com/prods/VOC-Products/Pages/HT3-Static-and-Dynamic-Headspace-System.aspx

https://www.thomassci.com/Laboratory-Supplies/General-Purpose-Syringes/_/ALL-PLASTIC-SYRINGES?q=sterile%20sample%20needles

https://www.hamiltoncompany.com/products/syringes-and-needles/syringe-selection-tool/25-microL-Model-1702-RN-SYR-Small-Removable-NDL-22s-ga-2-in-point-style-2

https://www.coleparmer.com/i/hamilton-1701-gastight-syringe-10-ul-cemented-needle-26-g-2-blunt-tip/0794011?PubID=UX&persist=true&ip=no&gclid=EAIaIQobChMIydTTmcGp3AIVhaDsCh0MuwuAEAQYAyABEgI6Z_D_BwE

https://www.gesswein.com/p-1385-mesh-heating-screens.aspx?gclid=EAIaIQobChMIy6Gy38Op3AIVRlp-Ch2c9gX7EAQYASABEgIz2vD_BwE

https://www.coleparmer.com/i/cps-products-vp6s-pro-set-single-stage-vacuum-pump-6-cfm-50-micron/0716488?PubID=UX&persist=true&ip=no&gclid=EAIaIQobChMI8unqlMSp3AIVEtNkCh3aigU_EAQYAiABEgKZAfD_BwE

https://www.sigmaaldrich.com/catalog/product/aldrich/z192597?lang=en&region=US

https://www.coleparmer.com/p/vortex-genie-2-shakers/63087