chick lab - society for developmental biology · chick cheap, easy to obtain fertilized eggs easy...
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Chick
Cheap, easy to obtain fertilized eggsEasy to work with on bench with dissecting microscopeSimilar development to human (flat disc)Genome - 1 x 109 bp (1/3 size of mouse and human); genome sequenceRapid functional tests
Microsurgery (cut & paste) to define cell interactionsMolecular manipulation - gain and loss of functionImaging, cell tracking
Fertilization
Hamburger and Hamilton Staging Stage 2 Stage 4
Early somite/heart fields
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HH Stage 7
HH Stage 14HH Stage 10
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Hensen’s node as an embryonic organizer
Prospective mesoderm cells ingress through the primitive streak in a rostral-caudalorder that presages their medial to lateral position after ingression
Node (most rostral) primitive streak= midline tissue
Rostral (anterior) primitive streak= paraxial (somite) tissue
Middle streak movements
Rapid lateral movement, pause, then back toward regressing node Lateral plate mesoderm
Yan, Dormann, Munsterberg and Weijer; Developmental Cell 3, 425-437, 2002
Caudal streak movements
Lateral and caudal movement
extraembryonicmesoderm
Cell autonomous or mediated by surrounding tissues?
Control Anterior streak to anterior streak
Anterior streakto caudal streak
Streak cells are attracted to FGF4
SU5402 = FGF inhibitor
Embryonic Polarity
Left-right
India ink
In Ovo Electroporation
In Ovo Electroporation
+ -
enhancer driven
constitutive promoter
RNAi