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Team Cherry PickerTeam Cherry Picker

Blake HondlBlake Hondl

Amit MehtaAmit Mehta

Jon MillinJon Millin

Ryan PopeRyan Pope

Team Small MoleculeTeam Small Molecule

Blake HondlBlake Hondl

Amit MehtaAmit Mehta

Jon MillinJon Millin

Ryan PopeRyan Pope

Client & AdvisorClient & Advisor

Noël R. Peters, M.S.Noël R. Peters, M.S.

Keck-UWCCC Keck-UWCCC

Small Molecule Screening Small Molecule Screening FacilityFacility

Professor Willis TompkinsProfessor Willis TompkinsDepartment of Biomedical Department of Biomedical

EngineeringEngineering

AbstractAbstract High throughput screening (HTS) involves High throughput screening (HTS) involves

secondary testing of chemicals that initially secondary testing of chemicals that initially show desired characteristicsshow desired characteristics– Done manually and prone to errorsDone manually and prone to errors

Goal is to design a device to guide a Goal is to design a device to guide a micropipette user to a single microtiter wellmicropipette user to a single microtiter well

The device will increase efficiency and reduce The device will increase efficiency and reduce user erroruser error

Device implementationsDevice implementations– Java ApplicationJava Application– LCD ScreenLCD Screen– User InterfaceUser Interface

Problem StatementProblem Statement Design a device to guide a Design a device to guide a

micropipette user in the transfer of micropipette user in the transfer of small volumes of compounds small volumes of compounds between 384-well microtiter platesbetween 384-well microtiter plates

The device will increase the The device will increase the efficiency of transfers and reduce efficiency of transfers and reduce user erroruser error

BackgroundBackground High Throughput High Throughput

ScreeningScreening– Used in cancer and Used in cancer and

RNA researchRNA research– Secondary testing Secondary testing

performed on the performed on the chemicals that chemicals that initially show initially show promisepromise

– Use of plate reader Use of plate reader to determine to determine potential “hits”potential “hits”

EnVison® Plate Reader

MotivationMotivation Secondary testing is typically done Secondary testing is typically done

manually, making it difficult and manually, making it difficult and prone to errorsprone to errors

Row and column identification is Row and column identification is necessarynecessary– Interior wells are difficult to locateInterior wells are difficult to locate

Errors lead to increased time costsErrors lead to increased time costs– Only step in the process that is prone to Only step in the process that is prone to

significant human errorsignificant human error

Current CompetitionCurrent Competition Matrix MemowellMatrix Memowell® ®

– 96 well pipetting 96 well pipetting aidaid

– Lights up 4 wells Lights up 4 wells when used with when used with 384-well plate384-well plate

– Costs approx. Costs approx. $1000$1000

Quadra Cherry Quadra Cherry PickerPicker– AutomatedAutomated– Costs $150,000Costs $150,000

http://www.matrixtechcorp.comhttp://www.tomtec.com

Design ConstraintsDesign Constraints Must interface with MicrosoftMust interface with Microsoft ExcelExcel and userand user Effectively guide the user to the appropriate Effectively guide the user to the appropriate

wellswells Minimize required bench spaceMinimize required bench space

– Dimensions smaller than 8.5” x 11” Dimensions smaller than 8.5” x 11” Withstand exposure to various chemicals and Withstand exposure to various chemicals and

reagentsreagents– Dimethyl SulfoxideDimethyl Sulfoxide

Lightweight (<10 lbs) and inexpensive Lightweight (<10 lbs) and inexpensive (<$1000)(<$1000)

Final DesignFinal Design Java ApplicationJava Application

– User input to locate wells in one or two User input to locate wells in one or two platesplates

– Excel input from spreadsheet (future Excel input from spreadsheet (future work)work)

LCD Screen to show output from Java LCD Screen to show output from Java Application Application – Lines (cross-hairs) displayed to identify Lines (cross-hairs) displayed to identify

wells to userwells to user

Final DesignFinal Design Protection of the LCD screenProtection of the LCD screen

– Hard acrylic sheet protects LCD screen Hard acrylic sheet protects LCD screen from pressure and possesses good optical from pressure and possesses good optical clarityclarity

– Polypropylene sheet to protect from the Polypropylene sheet to protect from the Dimethyl Sulfoxide bufferDimethyl Sulfoxide buffer

Maintaining the position of well plates Maintaining the position of well plates on LCD screenon LCD screen– Two sets of tracks guide the movement of Two sets of tracks guide the movement of

“arms” which will fix the plates in place“arms” which will fix the plates in place

Prototype ConstructionPrototype Construction Purchased Purchased

refurbished LCD refurbished LCD screenscreen– 10.4” Screen10.4” Screen– InverterInverter– Ribbon cableRibbon cable

Needs to be Needs to be refreshed at 60 Hzrefreshed at 60 Hz– Too fast for serial or Too fast for serial or

USB portUSB port

Prototype ConstructionPrototype Construction Acquired materialsAcquired materials

– Laptop: Samsung SENS 800Laptop: Samsung SENS 800– Aluminum sheet metalAluminum sheet metal– Polypropylene coverPolypropylene cover– Non-glare 1/8” acrylic sheetNon-glare 1/8” acrylic sheet

Detached screen from Detached screen from basebase– Purchased components to Purchased components to

extend screenextend screen 30 connector HD ribbon cable30 connector HD ribbon cable

ProgrammingProgramming User interfaceUser interface

– User may choose User may choose between:between: User input or User input or

MicrosoftMicrosoft ExcelExcel file file inputinput

Using one well plate Using one well plate or twoor two

Full screen displayFull screen display Displays Displays

rectangle for rectangle for definition of well definition of well plate boundariesplate boundaries

Displays Displays intersecting lines intersecting lines according to user according to user input, input, illuminating a illuminating a single wellsingle well

Well plate on LCD screen

Design AnalysisDesign Analysis AdvantagesAdvantages

– Ease of useEase of use– ManufacturingManufacturing– AdaptabilityAdaptability– CostCost– MarketabilityMarketability– Reduces errorsReduces errors– Increases Increases

productivityproductivity

DisadvantagesDisadvantages– Requires computer Requires computer

interface interface – Requires Requires

purchasing of purchasing of hardware as well as hardware as well as software.software.

– Software upgrades Software upgrades to enable to enable adaptabilityadaptability

Cost AnalysisCost Analysis Used Laptop from SWAPUsed Laptop from SWAP =$250.00 =$250.00 1/16” Aluminum Sheet (8 ft1/16” Aluminum Sheet (8 ft22)) =$33.36 =$33.36 Acrylic Sheet (2)Acrylic Sheet (2) =$ 3.90 =$ 3.90 Polypropylene coverPolypropylene cover =$ 0.20 =$ 0.20 Flat Ribbon Cable & ConnectorFlat Ribbon Cable & Connector =$16.71 =$16.71 Miscellaneous ComponentsMiscellaneous Components =$ 7.00=$ 7.00

Total=$311.17Total=$311.17 Cost to purchase a new LCD flat screen for a Cost to purchase a new LCD flat screen for a

computer $300 (Future Work)computer $300 (Future Work)

Future WorkFuture Work Implement additional programming codeImplement additional programming code

– Allow the program to take input from an ExcelAllow the program to take input from an Excel file file Conduct initial testing on laptop prototypeConduct initial testing on laptop prototype Create interface between prototype and PCCreate interface between prototype and PC Design an optimal structure for the prototype, Design an optimal structure for the prototype,

emphasizing ease of use for the useremphasizing ease of use for the user Confirm prototype’s resistance to chemical Confirm prototype’s resistance to chemical

solvents encountered in the labsolvents encountered in the lab

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http://www.viewsonic.com/monitoruniversity/lcdbasics.htm.http://www.viewsonic.com/monitoruniversity/lcdbasics.htm. ““LCD Video Controller.” Subassembly Product Guide. LCD Video Controller.” Subassembly Product Guide.

http://www.trans2000.com/manual/adboard_manual.pdf.http://www.trans2000.com/manual/adboard_manual.pdf. ““Matrix Memowell.” Matrix Technologies. http://www.matrixtechcorp.com. Retrieved Matrix Memowell.” Matrix Technologies. http://www.matrixtechcorp.com. Retrieved

on January 23, 2004.on January 23, 2004. Peters, Noël. Personal Interview. January 30, 2004.Peters, Noël. Personal Interview. January 30, 2004. ““Product Specification for LB104V03 Liquid Crystal Display.” Products Engineering Dept. Product Specification for LB104V03 Liquid Crystal Display.” Products Engineering Dept.

LG. Philips LCD Co., Ltd. http://www.jacoflatpanels.com/lcdpdfs/LB104V03- LG. Philips LCD Co., Ltd. http://www.jacoflatpanels.com/lcdpdfs/LB104V03-A1_CAS(Ver0.1).pdf.A1_CAS(Ver0.1).pdf.

““Quadra Cherry Picker.” Tomtec. http://www.tomtec.com. Retrieved on January 29, Quadra Cherry Picker.” Tomtec. http://www.tomtec.com. Retrieved on January 29, 2004. 2004.

““Samsung.” Samsung Electronics. http://www.samsungusa.com. Retrieved on April 22, Samsung.” Samsung Electronics. http://www.samsungusa.com. Retrieved on April 22, 2004.2004.

Schowalter, Jeff. Personal Interview. March 26, 2004.Schowalter, Jeff. Personal Interview. March 26, 2004. ““Small Molecule Screening Facility.” UW-CCC. Small Molecule Screening Facility.” UW-CCC.

http://www.medsch.wisc.edu/smsf/Index.htm. Retrieved on January 23, 2004. http://www.medsch.wisc.edu/smsf/Index.htm. Retrieved on January 23, 2004. Wu, Thomas C. “An Introduction to Object-Oriented Programming with Java (Third Wu, Thomas C. “An Introduction to Object-Oriented Programming with Java (Third

Edition)” and imported classes from JavaBook.Edition)” and imported classes from JavaBook.