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A bbott AnalyticalConsulting Scientists to the Disinfectant Industry
Certi£icate of AnaLysis
Sample(s): One sample of Sterizar
Received from: Creative Supply Solutions Ltd. Malvern Mill, South Wing,Earl Mill, Dowry St~eet, Oldham, OL8 2PF
Date received: 30 April 2010 Date tested: 14 May 2010
Certificate no: 100.140M20.CSS Certificate date: 17 May 2010
-~ Sample ref..:- 100/1-40 Page: 1 of 2
Analysis required: BS/EN 1650 quantitative suspension test for the evaluationof chemical disinfectants (yeast & mould)
Product stored at: Room temperature
Active substance: Not declared
Test conditions: 'Dirty'
Product test concentration: 1:20 v/v
Product diluent used duri~g test: Sterile hard water 300mg/l CaC03
Contact time: 15 minutes
Test temperat~re:
Interfering substance: 3g/1 bovine albumin
30g/1 polysorbate 80, 3g/1 lecithin,19/1 histidine, 19/1 cysteine
Neutralising solution:
I
Ii
Incubation temperature:
Identification of fungal strainsused:
Aspergillus nigerCandida albicans
NCPF 2275NCPF 3179
D C Watson
DC Watson BSc, CBio!, MlBiol, MIFST, ACTEHOPO Box 95, New Ferry, Wirral, CH62 6HA
Tel: 07767 871275 Fax: 01516306540email: [email protected]:
A bbott AnalyticalConsulting Scientists to the Disinfectant Industry
17 May 2010 Certificate No: 10D.140MN.CSS Page 2 of 2
Test resu1.ts:
ITest Organism Aspergi~~us Candida
I niger aThicans
~alidation10 -1 Vel 106 Ve2 72 Vel 416 Ve2 368
I/Suspension NvO 8.90 xlO 2 NvO 3.92 xlO 3
"'-- ~o .o¥eJ.......-M~C2-66- Ivci, 322-1 VeL. 254-iExperimental
IControl A. 7.50 xlO 1 A 2.88 xl02
II 100 Vel 76 Ve2 58 Vel 292 Ve2 346!INeutraliser
IControl B 6.70 xlO 1 B 3.19 xlO 2
kthOd100 Vel 56 Ve2 67 Vel 288 Ve2 362
!validation C 6.15 x101 C 3.25 xl02
10 -6 Vel 29 Ve2 38 Vel 340 Ve2 374
10 -7 Vel 4 Ve2 7 Vel 42 Ve2 51!TestISuspension N 4.43 x1G 7 N 4.11 x108
IResults
10 2 Vel 0 Ve2 0 Vel 0 Ve2 0
INa < 1.00 xlO 2 Na < 1.00 xlO 2
R > 4. 43 xlO 5 R >4.11 xlO 6
.,Log Reduction > 5. 65 > 6.61II
VcNv
Viable count;efu/ml in the validation suspension
Conc~usion:
N = efu/ml in the test suspensionNa = efu/ml in the test mixtureR = Reduetion in viabili~y
This batch of Sterizar, when used neat, passes the requirements of EN 1650 forfungicidal activity in 15 minutes at 20°C under 'dirty' conditions against thereference organisms detailed.
D C Watson
D C Watson BSc, CBiol, MIBiol, MIFST, ACIEHOPO Box 95, New Ferry, Wirral, CH62 6HA
Tel: 07767 871275 Fax: 0151 6306540email: [email protected]