Veterinary science and ”know how” adding value to your company

Christine Maria Røntved (CMR)

CMR On-Site RD (2011), located on AAU, Skjernvej 4Minor consultancy and RD company in the veterinary field

DVM and Ph.D from LIFE, Copenhagen University

Senior scientist at Aarhus University in Dep. of Animal Science, Immunology and Microbiology for 12 years

Expert area

• Disease models and disease monitoring in production animals

• Laboratory methods for measuring pathogen- and immune components in different animal body fluids and tissue compartments

• Several milk associated projects in dairy cattle

Somatic cells

Bacteria

Enzymes

Proteins

Selling analysis equipment for the Food Industry, Dairies, Meat and Fish plants

Serving Primary Producers in the Livestock and Fish production

Exploiting Animal By-products and Recycling Waste products

Product ideas, developments and

improvements that add value to companies

Fractionation of “animal by-products”

- the potential of membrane filtration

Animal By-products (ABP)

• Materials of animal origin – living or dead (waste milk, blood, parts of carcasses) that people do not consume

• Animal feed - e.g. based on fishmeal, processed animal protein and pet food

• Technical products (non-foodstuff) - e.g. hides and skins for leather, wool, blood for producing diagnostic tools.

• Over 20 million tons emerge annually from EU from slaughter-houses, plants producing food for human consumption, dairies and as fallen stock from farms.

Bovine colostrum an ABP from cattle

• First honey-like milk produced by the cow the first threedays after calving.

• Rich in immune components, growth factors and nutrientsimportant for the calves’s growth and local and systemicimmune defense

• Different composition than milk used for food consumption (waste product in some farms)

High levels of immune proteins in colostrum (200-fold)

1

10

100

1000

10000

100000

1000000

10000000

100000000

TGFb1 TGFb2 Hp MAA LBP Apo-L C1 LF Ig total

Ko

ncen

trati

on

, n

g/m

l

Proteiner i kolostrum

Kolostrum Mælk

Nissen et al., 2012, Proteomics

Colostrum applications

• Vital for feeding newborn calves (and in some cases othernew born animals)

• Dairy products - cheese in southern Europe

• Internal and external health care products developed for humans and pets (creme, dental paste, tablets, protein powder…)

• Purification of bioactive compounds: IgG, lactoferrin,

IGF-1, lactoperoxidase, lysozyme, osteopontin…

Colostrum fractionation

• In-depth analysis of low abundant proteins in bovine colostrum

using different fractionation techniques. (Nissen et al., 2012

Proteomics)

• 6 different fractionation techniques – 7 fractions

• Identification of 403 proteins by shotgun proteomics (2D-LC-MS/MS analysis)

• Colostrum whey (serum) highest

number identified (ca 110)Biological regulation

10%

Response to stimulus

9%

Regulation of biological

process

9%

Metabolic process

7%Localization

7%

Negative regulation of

biological process

4%

Immune system

process

4%

Establishment of

localization

6%

Multicellular organismal

process

6%

Positive regulation of

biological process

5%

Developmental process

5%

Cellular process

10%

Rhythmic process

<1%

Reproductive process

1%

Reproduction

1%

Growth

1%

Locomotion

1%

Multi-organism process

2% Cell killing

1%

Cellular component

organization

3%

Anatomical structure

formation

3%

Cellular component

biogenesis

2%

Biological adhesion

2%

Death

2%

•F7:

Combined

techniques

•Centrifuge

d•800g

•F4:

Whey

•acid

• F1:

Fatlayer

•F3: Cell

pellet

•NF: Non-

fractionated

•F2: Cell

and fat

free

•100 kDa /

•30 kDa

•pH = 4,6

•100 kDa

•100000g

•F5:

Whey

•filtrate

•F6: Whey

centrifuge

•3 •1

•4

•2

Colostrum fractionation

Nissen et al., 2012, Proteomics

•Figure 1A •Figure 1B

•Pool •Pool

•Whey

•fractionation

•2D-LC-MS/MS

•Analysis

•NF, F1, F2, F3

•F4, F5, F6, F7

•2D-LC-MS/MS

•Analysis

•Protein lists

•Bos taurus database

•Protein lists

•Bos taurus databaseModified from Nissen et al., 2012, Proteomics

Membrane filtration in the dairy industry

• Polymeric and ceramic microfiltration (MF) is used for bacteria and

spore removal - and are installed to provide improved quality of

pasteurized dairy products

• Opkoncentrering af ostemælk ved UF til hvide oste samt valleprotein

ved NF og RO.

• Mortensen U., 2003. http://www.patentgenius.com/patent/6521277.html

A process for the preparation of colostrum

• Piot et al., 2004 in Lait 84 p. 333-341

Preparation of serocolostrum by membrane microfiltration

Why filtration

• Low heat temperatures (pasteurization) do not kill/inactivate all bacteria and enzymes

• High heat temperatures (pasteurization) destroy the proteins and essential nutrients

• Possible separation, fractionation and concentration of specific nutrients in milk/colostrum

• AAU student project with ceramic filtration of milk and colostrum in collaboration with Vittorio Boffa, autumn 2012.

• New project application in the spring 2013

Christine Maria Røntved (CMR)

www.cmr-on-site.dk

http://www.linkedin.com/pub/christine-maria-r%C3%B8ntved/40/61a/930

http://www.ncbi.nlm.nih.gov/pubmed?term=rontved

http://www.forskningsdatabasen.dk ”Røntved”