Center of Excellence in Cancer Research, Tanta University

(CECR) مركز التميز ألبحاث السرطان بجامعة طنطا

CECR Training Program

The 1st CECR Training Program

Principles of Flow Cytometry

Principles of Flow Cytometry

Opening

CECR Introduction

Steps on Research Stairs

Lab Biosafety

Coffee Break

Introduction for Principles of Flow Cytometry.

Introduction for Clinical Applications of Flow Cytometry

Principles of Flow Cytometry

Opening

CECR Introduction

Steps on Research Stairs

Lab Biosafety

Coffee Break

Introduction for Principles of Flow Cytometry.

Introduction for Clinical Applications of Flow Cytometry

Principles of Flow Cytometry

Opening

CECR Introduction

Steps on Research Stairs

Lab Biosafety

Coffee Break

Introduction for Principles of Flow Cytometry.

Introduction for Clinical Applications of Flow Cytometry

Established Date

June 2013 through a project funded by Science and

Technology development Fund (STDF)

PI: Prof. Mohamed Labib Salem

Affiliation: Tanta University

Budget: 9,657,500 L.E

Duration: 36 Months

Established Date

Location

Tanta University Educational Hospital

Location

Prof. Mohamed L.Salem Director, CECR

Prof. Said Hammad Vise Director, CECR

Prof. Mohamed Ramadan Clinical Director, CECR

Prof. Mohamed Attia Director of Flow Cytometry Unit, CECR

Dr. Abdel-Aziz Zidan Director of Proteomic & Genomic Unit, CECR

Ms. Mona F. Zidan Research Assistant

Research Team

Eng. Heba M. Wassfy Coordinator , CECR

Dr. Enas Abdul-Raouf Research Assistant

Organogram

CECR Core Facilities

CECR Vision

Create omic based core facilities to lead cutting edge translational cancer research

CECR Mission

Create a rich academic environment for excellence in cancer research to lead the next generation of scientists and empower the discovery and entrepreneurships in cancer prognosis, diagnosis, prevention, and treatment through efficient collaboration with national and

international esteemed investigators.

CECR Goals

CECR GOALS

Training Outreach

Office

Research from bench to

clinic

Independent Researchers

Technical/ Clinical

Consultation

Grants Publications

Patents

Scientific Collaboration

Establish the (Center of Excellence in Cancer Research “CECR”) to perform cutting-edge preclinical and clinical reseacrh.

Correlating immunologic and clinical performance between genomic, transcriptomic and proteomic profiling of circulating tumor cells versus primary tumor cells

Two CECR Main Aims

CECR From Application to Funding

STDF-CSE Call

Proposals Evaluation

Interview & Oral Presentation

Site Visit

146 Project

61 Project

25 Project

CECR From Application to Funding

CECR From Application to Funding

CECR From Application to Funding

CECR Infrastructure

CECR Infrastructure

CECR Infrastructure

CECR Infrastructure

CECR Infrastructure

CECR Infrastructure

CECR Infrastructure

CECR Infrastructure

CECR Core Facilities

Immuno-Phenotyping Core Facility

Immuno-Phenotyping Core Facility

Flow Cytometry Unit

Cell Sorting Unit

Immuno-Phenotyping Core Facility

Flow Cytometry Unit

Cell Sorting Unit

Nomination: The BD FACSCanto derives its name from the musical term “bel canto” meaning

operatic singing that focuses on ease and purity of tone with an agile and precise vocal

technique.

Flow Cytometry Unit

• BD FACSCanto II Cytometer

Flow Cytometry Unit

FDA Approved

Research & Diagnosis

Stem cell enumeration

Detection of minimal

residual disease

BD FACSCanto Applications

Analysis of Leukemias and

Lymphomas

Reticulocyte analysis

Detection of

Immunodeficiency diseases

Detection of HIV infection

Analysis of different types of solid

tumors.

Detection of autoantibodies

Research & Clinical applications of the instrument

BD FACSCanto Applications

Immuno-Phenotyping Core Facility

Flow Cytometry Unit

Cell Sorting Unit

The IsoFlux system enriches rare cells from biological samples and prepare them for further analysis , It gives immediate access to high- quality , viable cells in a high density format , ready for use in the application of your choice

Cell Sorting Unit

Cell Sorting Unit

IsoFlux Applications

Enumeration Immunofluorescence Fluorescence in situ

hybridization

Next generation sequencing

Mutation detection Gene expression

Omic Core Facility

Omic Core Facility

Genomic Unit

Proteomic Unit

Omic Core Facility

Genomic Unit

Proteomic Unit

Microarray

A high throughput technology that allows detection of thousands of genes simultaneously.

Genomic Unit

Genomic Unit

Microarray Applications

Transiptome mRNA Expression miRNA Expression Splice Variance

Genome Structure DNA Copy number

Genome Variation Single Nucleotide Polymorphism

Copy number Variations

Proteome Protein-Protein Interactions DNA – Protein inreraction

Epigenetics DNA Methylation

Chromatin Structure

Agilent 2100 Bioanlayzer.

It is the industry standard for RNA sample QC and has replaced labor-intensive gel electrophoresis for this application, It is also rapidly replacing gel electrophoresis for DNA fragment analysis and SDS-PAGE analysis of protein samples.

Genomic Unit

Genomic Unit

Bioanalyzer Applications

Whole Cells

RNA

RNA Integrity RNA Qualification Small RNA Sizing

DNA DNA Integrity

DNA Qualification

Protein Protein Integrity

Protein Qualification Protein Purity

Gel Documentation System (Biometra Doc Analyze)

Enables simple documentation of fluorescent and non-fluorescent gels, membranes, blots, and assays with the ability to save images to disk for later quantitative analysis or enhancement for publication.

Genomic Unit

Gene AmpPCR System 9700

It is an automated instrument, specifically designed for the amplification of nucleic acids using the Polymerase Chain Reaction (PCR) process

Genomic Unit

Omic Core Facility

Genomic Unit

Proteomic Unit

• LumineX200

Proteomic Unit

Proteomic Unit

Luminex Applications

Clinical Diagnostics Clinical Research Life Science

Research

Biosurveillance Assay Development Tools

Cell Culture Core Facility

Biosafety Cabinet

Cell Culture Unit

Co2-Incubator

Centrifuge

CECR Publications

CECR Publications

The Use of Dendritic Cells for Peptide-Based Vaccination in Cancer Immunotherapy. Mohamed L. Salem

Immunostimulatory Effects of Triggering TLR3 Signaling Pathway Implication for Cancer Immunotherapy. Mohamed L. Salem, Said M.

Hammad, Mohamed R. Elshanshory, Mohamed A. Attia, Abdel-Aziz A. Zidan.

G-CSF/anti-G-CSF antibody complexes drive the potent recovery and expansion of CD11b+Gr-1+ myeloid cells without compromising CD8+ T cell immune responses. Mark P Rubinstein,

Mohamed L Salem, Andrew L Doedens, Caitlin J Moore, cody Chiuzan, Guillermo L Rivell, David J Cole and Ananda W Goldrath

CECR Publications

Understanding the biology of ex vivo-expanded CD8 T cells for adoptive cell therapy: role of CD62L Immunol Res. Díaz-Montero CM, Zidan AA, Pallin MF, Anagnostopoulos V, Mohamed L Salem, Wieder E, Komanduri K, Montero AJ, Lichtenheld MG

Immunobiology and signaling pathways of cancer stem cells: implication for cancer therapy. Salem ML, El-Badawy AS, Li Z

Autologous transplantation of CD34+ bone marrow derived mononuclear cells in management of non-reconstructable critical lower limb ischemia. Ismail AM1, Abdou SM, Aty HA, Kamhawy AH, Elhinedy

M, Elwageh M, Taha A, Ezzat A, Salem HA, Youssif S, Salem ML

CECR Publications

IL-12 enhances the function and anti-tumor activity in murine and human CD8+ T cells. Mark P. Rubinstein, Ee Wern Su, Samantha Suriano, Colleen A. Cloud, Kristina Andrijauskaite, Pravin Kesarwani, Kristina M. Schwartz, Katelyn Williams, C. Bryce Johnson, Mingli Li, Gina M. Scurti, Mohamed L. Salem, Chrystal M. Paulos, Shikhar Mehrotra, Elizabeth Garrett-Mayer, Michael I. Nishimura, and David J

CECR Collaborators

CECR Collaborators CECR Collaborators

CECR Activities

Monday 24 February 2014 The 1st Annual Conference of

The Center of Excellence in Cancer Research Next Generation Cancer Research & Diagnosis

In Collaboration with Research Centers of Excellence

(STDF- Affiliated Centers)

CECR Activities

CECR Activities

CECR Activities

RNA Extraction Training To CECR Research Team, CECR, Tanta University

Educational Hospital

CECR Activities

RNA Extraction Training To CECR Research Team, CECR, Tanta University

Educational Hospital

CECR Activities

Prof. Mohamed L. Salem’s visit to children Research Institute, Medical University of South Carolina, USA

CECR Activities

Prof. Mohamed L. Salem’s visit to Medial College of Georgia , Georgia Regents University, USA

CECR Activities

Prof. Mohamed L. Salem’s visit to Medial College of Georgia , Georgia Regents University, USA

CECR Activities

Prof. Mohamed L. Salem’s & Dr. Sohaila Galal’s visit to International 2013 Forum of Immunology. Montreal, Canada

CECR Activities

Prof. Mohamed Attia’s Training on FACSCanto Diva7 at BD Biosciences ,Heidelberg-Germany

CECR Activities

Dr. Abdel-Aziz Zidan Training Course on “ Affymetrix Geneship System 3000 7G , Stem Cell Center-Jordan University.

CECR Activities

Dr. Abdel-Aziz Zidan Training Course on “ Affymetrix Geneship System 3000 7G , Stem Cell Center-Jordan University.

CECR Activities

Mona Zidan’s Training course at International Center for Genetic Engineering & Biotechnology ICGEB “ Flow Cytometry & Cell Sorting in Biotechnology & Biomedicine Research” Institut Pasteur de Montevideo, Montevideo, Uruguay

CECR Activities

CECR Team Workshop on “The first workshop on Application of bioinformatics in Molecular Diagnostic Virology” . National Cancer Institute, Cairo University

CECR Activities

Dr. Sohaila Galal’s Workshop on “ Gene Expression on Large Scale Using Microarray and Real time PCR Techniques”. Cairo University Research Park

CECR Students

CECR Students

9 PhD. Students

15 MSc. Students

Adaptive Cell Therapy.

Cancer Biology

Anti Cancer effects of medicinal Plans

Stem Call based therapy

Cancer Stem cells

Immunoregulatory cells

Thank You !