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COMPETENCE IN TEXTILES

Cellular and Ecotoxicological Analysis of

Nanofunctionalized Textiles (Project TECHNOTOX)

Prof. Dr. Dirk Hfer

BfR, Berlin, February 9th 2012

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No. 2

Hohenstein InstituteDepartment Hygiene, Environment and Medicine

Hohenstein is a public and independent textile research institute.

Research & Development using public funding

Department Hygiene, Environment & Medicine

Life science studies on functionalized textiles

Efficacy and safety of products along the textile chain

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No. 3

Interaction Textile Human

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No. 4

Interaction Textile Environment

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No. 5

Silver and the human skin microflora

Field study with 60 subjects (21 65 years)

microbiological and dermatological supervision

Halved shirts (verum/placebo) were worn

for 8 h/d over a period of 5 weeks

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No. 6

Human skin microflora was not affected

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No. 7

Skin physiology experiments

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No. 8

Research project TECHNOTOX (2011 2013)

acc. to Krug & Wick (2011), Angew. Chem. 123(6): 1294-1314

Exposureassessment

Risk-characterization

Risk communication

Risk reduction

Risk prevention

Biologicalhazard

in vitro & in vivobioassays

physico-chemicalmodels

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No. 9

Nanomaterials in textiles

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No. 10

Use of organic biocides and silver in textiles

100 t/a organic biocides

28 t/a silver

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No. 11

Fibre functionalization with nanosilver

Surface modification

Material modification

Different abrasion behaviour Exposition

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No. 12

Textile abrasion

Mechanical stress (worst case scenario) Detection and collection

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No. 13

Distribution sites

Human

Eco

Source: PLoS Source: www.rad-zep.deSource: wikipedia

Food chain

skin alimentary tract Lung

bacteria DaphniaZebra fish

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No. 14

The lung as main entrance for nanoparticles

Size matters

from Krug & Wick (2011), Angew. Chem. 123(6): 1294-1314

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No. 15

TECHNOTOX lung model

37C heating plate

Aerosol generator Particle detectorflow chamber = Lung

30 min; 2,2 l/min

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No. 16

Lung model

Lung cell vitality after 30 min exposure to NP-aerosol

30% = Thresholdacc. to DIN EN ISO 10993-5

Zytotoxizitt der Lungenzellen nach Benebelung im Aerosolgenerator

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TiO2 ZnO AgPURE

Gro

wth

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[%

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Cell growth reduction after NP-aerosol exposure

TiO2 ZnO nAg (OECD NM300)

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No. 17

Cell toxicity studies according to DIN EN ISO 10993-5

Different cell types vary in their susceptibility!

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PK

Sol 1

1:2

Sol 1

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Sol 1

1:1

0

aus

Luft

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ne A

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PU

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7,5

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PU

RE

5 m

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AgP

URE

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PU

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iSyS

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Leber

Bindegewebe

Lunge

Makrophage / Immun

Liver

Connective tissue

Lung

Macrophages

POS Si-Sol1:2

Si-Sol1:4

Si-Sol1:10

nAg wash-out (air)

1:2

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