Cell cycle (Cell division), Cell-cell interaction, growth and their control

Aging Apoptosis Tissue injury and repair mechanisms Cell regenerating potential (Role of Stem Cells) Mediators, modulators and messengers

Antioxidants in health and disease

Core Course # MM 704

(Cellular and Molecular Pathology)

Induced differentiation of stem cells by Induced differentiation of stem cells by small moleculessmall molecules

Strategies to enhance regenerative Strategies to enhance regenerative potential of stem cellspotential of stem cells

Induced differentiation of stem cells by Induced differentiation of stem cells by small moleculessmall molecules

Direct Differentiation of ES CellsDirect Differentiation of ES Cells

Direct Differentiation of MSCsDirect Differentiation of MSCs

DexamethasoneAscorbic acid5-azacytidineZebularine Retinoic acidNitric oxideDifluoromethylornithine (DFMO)

Small Molecules and Cardiac DifferentiationSmall Molecules and Cardiac Differentiation

Zebularine

Differentiation of the Adult Stem Cells into Cardiac Differentiation of the Adult Stem Cells into Cardiac like cells by Small Moleculeslike cells by Small Molecules

Differentiation of the Adult Stem Cells into Cardiac Differentiation of the Adult Stem Cells into Cardiac like cells by Small Moleculeslike cells by Small Molecules

Cardiovasc Ther. 2012 Jun 15. doi: 10.1111/j.1755-5922.2012.00320.x

Cardiovasc Ther. 2012 Jun 15. doi: 10.1111/j.1755-5922.2012.00320.x

Differentiation of the Adult Stem Cells into Cardiac Differentiation of the Adult Stem Cells into Cardiac like cells by Small Moleculeslike cells by Small Molecules

Cardiovasc Ther. 2012 Jun 15. doi: 10.1111/j.1755-5922.2012.00320.x

Differentiation of the Adult Stem Cells into Cardiac like cells Differentiation of the Adult Stem Cells into Cardiac like cells by Small Moleculesby Small Molecules

Cardiovasc Ther. 2012 Jun 15. doi: 10.1111/j.1755-5922.2012.00320.x

Strategies to enhance regenerative potential Strategies to enhance regenerative potential of stem cellsof stem cells

(a) Hypoxic Preconditioning(a) Hypoxic Preconditioning

After HypoxiaAfter Hypoxia

Before HypoxiaBefore Hypoxia

After ReoxygenationAfter Reoxygenation

(b) Genetic Manipulation(b) Genetic Manipulation

e.g. Viral mediated e.g. Viral mediated

Engineered MSCs transdifferentiate into CMs

Circulation Res. Vol 99, 776-84. 2006

Transplantation of Modified Stem Cells

Scar tissue

Heart Attack

Blocked blood vessel

Dead muscle

Myocardial infarction

Isolation of Mesenchymal Isolation of Mesenchymal Stem Cells from ratsStem Cells from rats

Infarction Model in Infarction Model in RatsRats

Functional Functional StudiesStudies

Cell Cell TransplantationTransplantation

HistologHistologyy

RatRat

Chemical or Genetic Chemical or Genetic ModificationModification

Cell Transplantation

Myogenic transdifferentiationof MSC at 4 weeks after cell transplantation

FITC actinin staining Red PKH26 staining

DAPI Nuclear staining Merge

Circulation Res. Vol 99, 776-784. 2006

ReferencesReferences

Cardiovasc Ther. 2012 Jun 15. doi: 10.1111/j.17555922.2012.00320.x

Circulation Res. Vol 99, 776-84. 2006