cell culture products

Cell Culture Products

www.paa.com

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Your worldwide supplierof Cell Culture Products

www.paa.com

North America

PAA Laboratories Inc.145 Bethridge RoadEtobicoke, OntarioM9W 1N4

Tel. (+1) 416 744 8996Fax (+1) 416 744 8401Toll free 1 800 470 [email protected]

France

PAA Laboratories67 rue Aristide Briand78130 Les Mureaux

Tel. (+33) 1 300 415 03Fax (+33) 1 300 415 [email protected]

Australia

PAA Laboratories Pty Ltd46 Steel PlaceMorningside, QLD4170

Tel. (+61) 7 38 99 39 41Fax (+61) 7 38 99 39 [email protected]

Headquarters – Austria

PAA Laboratories GmbHHaidmannweg 9A-4061 Pasching

Tel. (+43) 72 29-6 48 65Fax (+43) 72 29-6 48 [email protected]

Germany

PAA Laboratories GmbHUnterm Bornrain 2D-35091 Cölbe

Tel. (+49) 64 21-1 75 39-0Fax (+49) 64 21-1 75 [email protected]

United Kingdom

PAA Laboratories LtdTermare CloseHoundstone Business ParkYeovil, Somerset BA22 8YG

Tel. (+44) 1935 411 418Fax (+44) 1935 411 [email protected]


www.paa.com

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“”

Customer Service is the focus

of our effortsRainer C. BurianPresident & CEO

North America


France


Australia




Germany


United Kingdom


3PAA – The Cell Culture Company | www.paa.com

Dear Customer

manufacturing sites in Brisbane (Australia) andToronto (Canada) to our corporate manufacturingoperation in Linz.

As part of this acquisition the Cansera brand of cellculture products was incorporated into the PAA brandthereby giving our ‘new’ North American, and manyother customers, access to a more comprehensiverange of products.

In parallel with our investment in facilities & capabili-ties we have also committed considerable funds intonew product development to satisfy the requests ofour research based customers. We have increased ourresearch product ranges with the addition of new andinnovative media, detachment factors as well as seve-ral new reagents.

To our existing customers I sincerely hope you enjoyreading this new PAA catalogue and find the additionsto be both exciting and useful in your specific areas ofcell biology research, diagnostics and production.

To our new customers: Welcome to the world of PAA“The Cell Culture Company”.

Thanks to you all for your continuing support andconfidence.

PAA is a leading manufacturer and supplier of cell cul-ture products for research, development, diagnosticand biopharmaceutical production. We have come along way from our modest beginning in the 1980’swhen a small, but enthusiastic, group of people startedto manufacture a narrow range of reagents for useprincipally with research based customers.

Over the course of the last 20 years we have growninto an international operation with manufacturingand sales on three continents but will never forget ourfundamental business customers in the research anddevelopment laboratories.

In 2003 PAA opened its new, state-of-the-art, produc-tion facility close to Linz, Austria. This production faci-lity operates to cGMP standards and, in 2005, wasgranted a pharmaceutical level licence by the AustrianMinistry of Health. Therefore PAA is now the only cellculture production facility which produces cell cultureproducts under a GMP “in vivo” pharmaceutical licen-ce. We are the “Cell Culture Company” and we willcontinue with this tradition.

During 2005, PAA purchased the serum manufactu-ring facilities of Serono, Switzerland. This acquisitionhas given us a truly worldwide presence by adding

Rainer C. BurianPresident & CEO

4 PAA – The Cell Culture Company | www.paa.com

Table of Contents

Classical Media 14

DMEM low Glucose (1 g/l) . . . . . . . . . . . . . . . . . 14DMEM high Glucose (4.5 g/l) . . . . . . . . . . . . . . . 15DMEM Thermostable Medium . . . . . . . . . . . . . . . 16DMEM/Ham's F-12 . . . . . . . . . . . . . . . . . . . . . . . 17Ham's F-10 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18Ham's F-12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19Iscove's modified DMEM (IMDM) . . . . . . . . . . . . . 20L-15 Medium (Leibovitz's) . . . . . . . . . . . . . . . . . . 21Medium 199 with Earle's Salts . . . . . . . . . . . . . . . 22Medium 199 with Hanks' Salts . . . . . . . . . . . . . . 23MEM Alpha Modification . . . . . . . . . . . . . . . . . . 24Minimal Essential Medium Eagle (MEM) . . . . . . . . 25RPMI 1640 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26RPMI 1640 Thermostable Medium . . . . . . . . . . . . 27DMEM Ready Mix . . . . . . . . . . . . . . . . . . . . . . . . 28RPMI 1640 Ready Mix . . . . . . . . . . . . . . . . . . . . . 29

Cytogenetic Media 30

Quantum 3-21 . . . . . . . . . . . . . . . . . . . . . . . . . . 30Quantum PBL . . . . . . . . . . . . . . . . . . . . . . . . . . . 31Ham's F-10 Ready Mix . . . . . . . . . . . . . . . . . . . . 32

Neuronal Media 33

Neuronal Base Medium . . . . . . . . . . . . . . . . . . . . 33Neuronal Base Medium AD . . . . . . . . . . . . . . . . . 34

Stem Cell Media 35

CollagenStem Kit . . . . . . . . . . . . . . . . . . . . . . . . 35MethoStem Medium . . . . . . . . . . . . . . . . . . . . . . 36MesenchymStem Medium . . . . . . . . . . . . . . . . . . 37

Cell Type Specific Media 38

Endothelial Cell Medium . . . . . . . . . . . . . . . . . . . 38Endothelial Basal Medium . . . . . . . . . . . . . . . . . . 38Endothelial Medium Supplement . . . . . . . . . . . . . 38Macrophage Medium . . . . . . . . . . . . . . . . . . . . . 39Hybridoma Express . . . . . . . . . . . . . . . . . . . . . . . 40Insect Express Sf9-S2 . . . . . . . . . . . . . . . . . . . . . 41TC 100 Medium . . . . . . . . . . . . . . . . . . . . . . . . . 42Quantum 101 for HeLa Cells . . . . . . . . . . . . . . . . 43Quantum 263 for Tumour Cells . . . . . . . . . . . . . . 44Quantum 286 for Epithelial Cells . . . . . . . . . . . . . 45Quantum 333 for Fibroblasts . . . . . . . . . . . . . . . . 46

Foetal Bovine Serum (FBS) 50

FBS Gold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50FBS Standard Quality . . . . . . . . . . . . . . . . . . . . . 51FBS PAA Clone . . . . . . . . . . . . . . . . . . . . . . . . . . 51FBS Gamma Irradiated . . . . . . . . . . . . . . . . . . . . 52FBS Heat Inactivated . . . . . . . . . . . . . . . . . . . . . . 53FBS IgG Stripped . . . . . . . . . . . . . . . . . . . . . . . . . 54FBS Dialysed . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54FBS ES cell tested . . . . . . . . . . . . . . . . . . . . . . . . 55FBS Amnion cell tested . . . . . . . . . . . . . . . . . . . . 55FBS Tetracycline negative . . . . . . . . . . . . . . . . . . . 56FBS Mycoplex . . . . . . . . . . . . . . . . . . . . . . . . . . . 56FBS Pharma Grade . . . . . . . . . . . . . . . . . . . . . . . 57

Bovine Sera 58

Neo-natal FBS . . . . . . . . . . . . . . . . . . . . . . . . . . . 58Newborn Calf Serum . . . . . . . . . . . . . . . . . . . . . 59Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60Donor Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . 60Bovine Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . 61Donor Bovine Serum . . . . . . . . . . . . . . . . . . . . . . 61

Horse Sera 62

Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . 62Donor Horse Serum . . . . . . . . . . . . . . . . . . . . . . 62

Other Animal Sera 63

Porcine Serum . . . . . . . . . . . . . . . . . . . . . . . . . . 63Sheep Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . 63Rabbit Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . 63Goat Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63Rat Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63Mouse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Human Sera 64

Human Serum, off the clot . . . . . . . . . . . . . . . . . 64Human Serum, converted . . . . . . . . . . . . . . . . . . 65

Media Sera


Table of Contents

Amino Acids & Vitamins 70

MEM Amino Acid Concentrate . . . . . . . . . . . . . . 70NEAA Amino Acid Concentrate . . . . . . . . . . . . . . 70L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71Stable Glutamine . . . . . . . . . . . . . . . . . . . . . . . . 71L-Glutamine with Pen/Strep . . . . . . . . . . . . . . . . . 72MEM Vitamins . . . . . . . . . . . . . . . . . . . . . . . . . . 72

Antibiotics 73

Amphotericin B . . . . . . . . . . . . . . . . . . . . . . . . . 73Antibiotic-Antimycotic Solution . . . . . . . . . . . . . . 73Gentamicin . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74Penicillin/Streptomycin . . . . . . . . . . . . . . . . . . . . 74MycoKill AB . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

Detachment 76

Accutase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76Alfazyme . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76AccuMax . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77Collagenase 2 . . . . . . . . . . . . . . . . . . . . . . . . . . 77Trypsin . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 - 81

Growth Supplements 82

Hybridoma Cloning Factor . . . . . . . . . . . . . . . . . . 82HematoStem . . . . . . . . . . . . . . . . . . . . . . . . . . . 82B27 NeuroMix . . . . . . . . . . . . . . . . . . . . . . . . . . 83G5 Supplement . . . . . . . . . . . . . . . . . . . . . . . . . 83N2 Supplement . . . . . . . . . . . . . . . . . . . . . . . . . 84Neuronal Stem Cell Supplement . . . . . . . . . . . . . 84

Transfection 85

Nanofectin Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 85G-418 Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . 86Puromycin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87Blasticidin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87Hygromycin B . . . . . . . . . . . . . . . . . . . . . . . . . . . 88Carbenicillin . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

Other Reagents 89

Lymphocyte Separation Medium . . . . . . . . . . . . . 89Bovine Serum Albumin . . . . . . . . . . . . . . . . . 90 - 92Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . 92Giemsa Stain Solution . . . . . . . . . . . . . . . . . . . . . 93Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . 93Colcemid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94Phytohaemagglutinin (PHA) . . . . . . . . . . . . . . . . . 94CryoMaxx . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95CoolStar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

Basalt Salt Solutions 100

Dulbecco's Phosphate Buffered Saline (DPBS) . . . . 100Hanks' Balanced Salt Solution (HBSS) . . . . . . . . . 101Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . 102HEPES Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . 102

Rinsing Solution 103

Cool Star Rinse . . . . . . . . . . . . . . . . . . . . . . . . . 103

Water 103

Water, AP Grade . . . . . . . . . . . . . . . . . . . . . . . . 103

Reagents Buffer Solutions


Table of Contents

Cytogenetics 105

Quantum 3-21 . . . . . . . . . . . . . . . . . . . . . . . . . 108Quantum PBL . . . . . . . . . . . . . . . . . . . . . . . . . . 109Ham's F-10 Ready Mix . . . . . . . . . . . . . . . . . . . 110FBS Amnion cell tested . . . . . . . . . . . . . . . . . . . 111Colcemid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . 112Giemsa Stain Solution . . . . . . . . . . . . . . . . . . . . 112Phytohaemagglutinin (PHA) . . . . . . . . . . . . . . . . 112

Transfection 113

Nanofectin Kit . . . . . . . . . . . . . . . . . . . . . . . . . 116G-418 Sulphate . . . . . . . . . . . . . . . . . . . . . . . . 117Hygromycin B . . . . . . . . . . . . . . . . . . . . . . . . . . 118Blasticidin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119Carbenicillin . . . . . . . . . . . . . . . . . . . . . . . . . . . 120Puromycin . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120

Neuronal Cell Culture 121

Neuronal Base Medium . . . . . . . . . . . . . . . . . . . 125Neuronal Base Medium AD . . . . . . . . . . . . . . . . 126B27 NeuroMix . . . . . . . . . . . . . . . . . . . . . . . . . 127Neuronal Stem Cell Supplement . . . . . . . . . . . . 128G5 Supplement . . . . . . . . . . . . . . . . . . . . . . . . 129N2 Supplement . . . . . . . . . . . . . . . . . . . . . . . . 130

Stem Cell Culture 131

CollagenStem Kit . . . . . . . . . . . . . . . . . . . . . . . 135MethoStem Medium . . . . . . . . . . . . . . . . . . . . . 135MesenchymStem Medium . . . . . . . . . . . . . . . . . 136Neuronal Base Medium . . . . . . . . . . . . . . . . . . . 136FBS ES cell tested . . . . . . . . . . . . . . . . . . . . . . . 137FBS Pharma Grade . . . . . . . . . . . . . . . . . . . . . . 138Human Serum, off the clot . . . . . . . . . . . . . . . . 139Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . 140HematoStem . . . . . . . . . . . . . . . . . . . . . . . . . . 140Neuronal Stem Cell Supplement . . . . . . . . . . . . 141B27 NeuroMix . . . . . . . . . . . . . . . . . . . . . . . . . 141G5 Supplement . . . . . . . . . . . . . . . . . . . . . . . . 142N2 Supplement . . . . . . . . . . . . . . . . . . . . . . . . 142

Cell Preservation 143

CryoMaxx S and SF . . . . . . . . . . . . . . . . . . . . . . 146CryoMaxx I and II . . . . . . . . . . . . . . . . . . . . . . . 147CoolStar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148

Alphabetical Index 149

Applications Index


PAA – The Cell Culture Company

To our present headquarters in Pasching (Austria) andour European subsidiaries based in Germany, Franceand UK, we now introduce to you our production faci-lities in Toronto (Canada) and Brisbane (Australia). PAAis a ‘truly international’ operating company.

Our German subsidiary is also responsible for sales toThe Netherlands, Hungary, Austria and Switzerland. Inaddition it has corporate responsibility for businessdevelopment, marketing and technical services.

Through the services of local partners we are repres-ented in over 40 countries worldwide. For a completelisting of our international distributor network pleaserefer to our website www.paa.com.

Technical Service

Our technical support team has a great deal of know-ledge and experience in cell culture technology. Thisteam, together with a group of dedicated scientificstaff, can provide you with product guidance, expertadvice, troubleshooting, tips and protocols for all PAAproducts. Please contact your local PAA office orworldwide partner who will then forward your requestto the appropriate technical service person.

Introduction


How to use this Catalogue

To simplify navigation around the cell culture chaptersof this catalogue we have colour coded the four mainsections. You will find our

Cell Culture Media

Sera

Reagents

Buffer Solutions

easily identifiable by their respective chapter colours.

New to our catalogue is the classification of some pro-ducts into five additional sections. This will provide youwith a rapid and effective overview in the areas of

Cytogenetics

Transfection

Neuronal Cell Culture

Stem Cell Culture

Cell Preservation

They are marked in a grey coloured register eachhaving its own topic specific symbol.

For Cytogenetic diagnosis we offer, along with ourQuantum 3-21 and Quantum PBL, two new completemedia to help you prepare clear karyograms for chro-mosomal analysis.

In our Transfection section, in conjunction with ourtransfection antibiotics, you will find Nanofectin, anew reagent based on nanotechnology principals.Nanofectin is taken up by cells very efficiently and pro-tects the bound DNA against endonucleasedegradation.

Neuronal and Stem Cells are very demanding andrequire precise cell culture conditions. PAA hasdeveloped a number of specialised media togetherwith specific supplements for optimal cultivation ofthese cells.

PAA's products for Cell Preservation protect cellsduring low temperature storage and provide you withmaximum cell viability and recovery after thawing.

Rapid Product Search can be performed by

> Examining the table of contents shown at the begin-ning of this catalogue

> Studying the individual chapter index page

> Using the alphabetical index shown at the end ofthis catalogue

Our website www.paa.com has an extended searchfunction enabling you to find all available products,product information, various protocols, certificates ofanalysis together with a wide range of technical sup-port issues to help you with successful cell culture.

Should you have any further questions than please feelfree to contact our customer service or technicalsupport team.

Cytogenetics

Transfection


Stem Cell Culture

Cell Preservation

Media

Sera

Reagents

Buffer Solutions


With over 20 years experience PAA has an in-depthknowledge in the development, production and distri-bution of cell culture and diagnostic products. We pos-sess the technical and scientific know-how to solvecustomer specific problems in terms of special productmanufacturing. We focus on customer service and arededicated to prove this every day in meeting your spe-cific demands.

Our Expertise

Although PAA has a wide range of media and supple-ments we realize that our customers have their ownspecific cell lines, clones and targets of interest. Thesemay not be served by standard media. Our experiencein developing and manufacturing cell culture productssuch as serum, media and reagents provides us withvast insight into regulatory-friendly design, manufactu-re and support of customized products. When yourproject requirements go beyond our standard catalo-gue products we are the right choice for you. We dedi-cate research and development resources to manufac-ture media or to provide other solutions according toyour specific needs. We can also offer a range of spe-cially treated serum products which are not listed inour standard catalogue.

The Packaging

PAA offers a variety of options for packaging of liquidmedia, buffer and other solutions, including bulk. Ourexperience in packaging allows us to select the highestquality and most reliable disposable packaging com-ponents for the delivery of media. We fill customerspecific formulations from 20ml up to 2000ml in PET

bottles. Larger volumes from 5 to 20 litres are filled inflexible plastic bags. As an alternative we also offer ste-rile media, buffers, washing solutions and ready to usesolutions filled in low cost cubitainers. Should yourneeds require something other than our standardpackaging; we will design a customized package spe-cific to your requirements. All solutions are filled undercGMP clean room conditions.

Cell Culture Media

We are customization experts for modified media for-mulations. PAA produces customer specific normal cellculture media with small modifications (e.g. methioninefree media or addition of special supplements), mediafor specific metabolic needs, such as adjustments tokey components i.e. glucose, amino acids and growthfactors and media with complex ingredients. We alsoproduce media which are totally free of animal derivedcomponents for biopharmaceutical purposes.

Confidentiality Agreement

PAA has a complete understanding of the confidentialnature of our clients development projects. All infor-mation provided to PAA is handled in a confidentialmanner and is distributed internally on controlled docu-ment basis only. By signing a two-way ConfidentialityDisclosure Agreement we guarantee that your intellec-tual property will not be compromised. For additionalinformation on media development and/or media opti-mization at PAA, please contact your PAA AccountManager or e-mail us at [email protected]. Youwill find a standard agreement on our webpage underwww.paa.com/confidential.html.

Customized Products


The Product Range

PAA manufactures on request a large variety of diffe-rent cell culture products. See below a small selection:

> Standard media with or without specific components

> Buffers as low volume (10 ml - 20 ml) washingsolutions for automated clinical analysis

> Rinsing solutions for medical analysis instruments

> Serum free media (also in large pack sizes e.g. 100 l)

> Indicator solutions for the diagnostic industry

> γ-irradiated cleaning solutions in spray bottles

How to place a Customer specific Order

A Custom Order is defined as a request for productswith formulation, packaging and/or testing require-ments other than that of the product offered throughthe current PAA catalogue. Custom orders requireclear information concerning:

> The formulation

> The packaging requirements

> The total volume requirements

> The testing requirements (if differing from our stan-dard testing)

> Terms and conditions

In many cases you can identify your customized pro-duct within the product range of PAA with only minorchanges to the standard formulation.

Also if your formulation is not a modification of a stan-dard catalogue product but a new formulation you

need to supply us a complete detailed formulationincluding product source if you wish that we use com-ponents of a certain origin.

For details please refer to the downloadable form (CP=customized product) you find on our homepage underwww.paa.com/customized.html.

Please consult our technical service([email protected]) for information regardingyour customized media requirements or to facilitatecompleting your request.

Terms and Conditions

Orders cannot be cancelled once a purchase ordercommitment has been signed.

Changes made to lot size, packaging or testing mustbe communicated in writing prior to production dateand may be subject to price adjustments.

In the event of a product overage in manufacturing,PAA is allowed to ship up to 5 % in excess of your totalorder and consider it complete at that time.

Minimum Production Volume

PAA's minimum production volume for customizedmedia is 10 litres. The pack size is independent fromthe total ordered volume.

Quality Control and Special Testing

Our standard quality control testing includes osmolali-ty, endotoxin, pH and sterility. If any additional or alter-native tests are required then please consult our tech-nical service at [email protected] and provideyour specific testing requirements.

In general you can be assured that we are able to pro-vide a wide range of common analytical validated teststo suit your requirements.

OEM Production

PAA has many years of experience in OEM production.As a special service we offer the production of OEMstandard and customized products for the industryunder their own label or with neutral label.

Your Idea – our Support for Product Realisation

You have found an interesting new media formulationwith much better growth features?

You think this media could also be of interest for otherpotential users?

PAA supports you in the product development, manu-facturing and marketing. Our cGMP production facili-ty at Pasching, Austria and our world-wide active salesrepresentatives can turn your idea into a commerciallysuccessful product.

For further information contact us by email [email protected].

1. Download the form (CP) http://www.paa.com/customized.html

2. Please complete the form and price request asrequired

3. Fax the completed and signed form to PAA

4. You will receive a rapid response together with anoffer

5. If you agree you sign the purchase order commit-ment

6. PAA gives you a reliable and agreed delivery dateand begins production

7. As soon as our quality control has approved the pro-duct we ship it to you or alternatively stock it for you.Parallel QC testing by customer can be arranged

General procedure to order a custo-mized product is as follows:


Media

Med

ia

Classical Media 14

DMEM low Glucose (1 g/l) . . . . . . . . . . . . . . . 14DMEM high Glucose (4.5 g/l) . . . . . . . . . . . . . 15DMEM Thermostable Media . . . . . . . . . . . . . . 16DMEM/Ham's F-12 . . . . . . . . . . . . . . . . . . . . 17Ham's F-10 . . . . . . . . . . . . . . . . . . . . . . . . . . 18Ham's F-12 . . . . . . . . . . . . . . . . . . . . . . . . . . 19Iscove's modified DMEM (IMDM) . . . . . . . . . . 20L-15 Medium (Leibovitz's) . . . . . . . . . . . . . . . 21Medium 199 with Earle's Salts . . . . . . . . . . . . 22Medium 199 with Hanks' Salts . . . . . . . . . . . . 23MEM Alpha Modification . . . . . . . . . . . . . . . . 24Minimal Essential Medium Eagle (MEM) . . . . . 25RPMI 1640 . . . . . . . . . . . . . . . . . . . . . . . . . . 26RPMI 1640 Thermostable Medium . . . . . . . . . 27DMEM Ready Mix . . . . . . . . . . . . . . . . . . . . . 28RPMI 1640 Ready Mix . . . . . . . . . . . . . . . . . . 29

Cytogenetic Media 30

Quantum 3-21 . . . . . . . . . . . . . . . . . . . . . . . 30Quantum PBL . . . . . . . . . . . . . . . . . . . . . . . . 31Ham's F-10 Ready Mix . . . . . . . . . . . . . . . . . . 32

Neuronal Media 33

Neuronal Base Medium . . . . . . . . . . . . . . . . . 33Neuronal Base Medium AD . . . . . . . . . . . . . . 34

Stem Cell Media 35

CollagenStem Kit . . . . . . . . . . . . . . . . . . . . . . 35MethoStem Medium . . . . . . . . . . . . . . . . . . . 36MesenchymStem Medium . . . . . . . . . . . . . . . 37

Cell Type Specific Media 38

Endothelial Cell Medium . . . . . . . . . . . . . . . . 38Endothelial Basal Medium . . . . . . . . . . . . . . . 38Endothelial Medium Supplement . . . . . . . . . . 38Macrophage Medium . . . . . . . . . . . . . . . . . . 39Hybridoma Express . . . . . . . . . . . . . . . . . . . . 40Insect Express Sf9-S2 . . . . . . . . . . . . . . . . . . . 41TC 100 Medium . . . . . . . . . . . . . . . . . . . . . . 42Quantum 101 for HeLa Cells . . . . . . . . . . . . . 43Quantum 263 for Tumour Cells . . . . . . . . . . . 44Quantum 286 for Epithelial Cells . . . . . . . . . . 45Quantum 333 for Fibroblasts . . . . . . . . . . . . . 46


Med

ia

MediaMedia provide basic nutrients for all cell cultureapplications. Therefore PAA offers high qualitymedia based on pharmaceutical grade raw materialand reliable production procedures.

13

Med

ia

Media of the highest quality are essential for good cellculture. To prepare high quality media, PAA uses onlyraw materials suitable for use in cell culture. All rawmaterials, purified water and production procedures arecarefully controlled to ensure that the cell culture mediameet the precise requirements of the customers.

Raw Materials

All components (chemicals, carbohydrates, aminoacids, vitamins, etc.) used in the production of cell cul-ture media are carefully selected and pretested to meetour strict and fully documented internal QualityControl & Quality Assurance (QC/QA) requirements.The water used to prepare liquid media is purified (APgrade). Only raw materials which meet with PAA stan-dards are released for use in the media productiondepartment.

Manufacturing

All PAA cell culture media are manufactured understrict Standard Operating Procedures (SOP's). Controlsof important parameters such as endotoxin, pH andosmolality are performed prior to sterile filtrationthrough 0.2 µm filters. Media are then bottled on com-puter controlled automatic filling lines. Every batch isthen placed in quarantine until QC tests are performedand pass results obtained. Only batches which pass ourstrict QC are released for sale.

Certificates of Analysis and full batch documentationare prepared for each batch.

Packaging

All sterile filtered liquid media are filled into PET bottlesof 500ml of 100ml volumes. Customized fillings areavailable from 25ml to 1000ml.

For further information see page 9 “CustomizedProducts”.

Storage and Shipping

The majority of PAA's cell culture media should bestored upon receipt at a temperature of between +2°Cand +8°C. A short term storage of these media at roomtemperature is possible. All media are shipped atambient temperature.

A few media should be stored at ≤ -15 °C. Thesemedia will be shipped in styrofoam boxes containingdry ice.

L-Glutamine

L-Glutamine is an essential amino acid required for cellculture. L-glutamine is a thermo sensitive amino acidwhich can degrade in storage or during incubation. By-products of degraded L-glutamine such as NH4

+ candamage cell membranes, particularly in serum freemedia.

To avoid this problem PAA recommends one of thetwo following alternatives:

1. Addition of fresh L-glutamine to the medium at thetime of use. PAA supplies frozen L-glutamine whichcan be used for this purpose.

2. The choice of a medium containing a stabilizeddipeptide form of L-glutamine, L-alanyl-L-glutaminewhich can be metabolized by many cells the sameas L-glutamine.

PAA has developed a number of cell culture mediacontaining this thermostable form of L-glutamine.

Introduction

PAA – The Cell Culture Company | www.paa.com

Dulbecco's modified Eagle's Medium (DMEM), low Glucose (1 g/l)


Med

ia

Cat. No. Volume

DMEMwithout L-Glutamine E15-005 500 ml

DMEMwith L-Glutamine E15-806 500 ml

DMEMwith 25 mM HEPESwithout L-Glutamine E15-007 500 ml

DMEMwith 25 mM HEPESwith L-Glutamine E15-808 500 ml

Applications

> Originally used for the cultivation of embryonic mouse cells> Supports the growth of most cell lines> Liver cells (mouse), endothelial cells> Recommended for use with 10 % supplementation of

FBS

Features

> DMEM is a modification of the Eagle Medium > Contains a fourfold concentration of amino acids> Contains vitamins and additional components > Original composition of DMEM contains 1 g/l glucose

Product Specifications

CO2-Concentration, optimum . . . . . . . . . . . . . 8.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/ml

Cell Culture . . . . . . . . . . . . . . . . . . . . . . . . testedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . without L-Glutamine 18 months . . . . . . . . . . . . . . . . . . . . . with L-Glutamine 12 monthsStorage . . . . . . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Ferric(III)-Nitrate • 9H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 97.70Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6400.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 125.00Sodium Hydrogen Carbonate . . . . . . . . . . . . . . . . . . . . 3700.00

Amino AcidsL-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00L-Glutamine in E15-806 and E15-808 . . . . . . . . . . . . . 584.00Glycine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Phenylalanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Serine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.00

VitaminsD-Calcium-Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Choline Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.20Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.40Thiamine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . . 1000.0HEPES in E15-007 and E15-808 . . . . . . . . . . . . . . . . . 5958.00Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00

Dulbecco's modified Eagle's Medium (DMEM), high Glucose (4.5 g/l)


Med

ia

Cat. No. Volume

DMEMwithout L-Glutamine E15-009 500 ml

DMEMwith L-Glutamine E15-810 500 ml

DMEMwith Stable Glutamine E15-883 500 ml

DMEMwith Sodium Pyruvatewithout L-Glutamine E15-011 500 ml

DMEMwith Sodium Pyruvatewith L-Glutamine E15-843 500 ml

DMEMwithout Phenol Redwithout L-Glutamine E15-047 500 ml

DMEMwith D-Valinewithout L-Valinewithout L-Glutamine E15-055 500 ml

DMEM without Phenol Redwith L-Glutamine E15-877 500 ml

DMEMwithout Calciumwithout L-Glutamine E15-078 500 ml

DMEMwithout Glucosewithout L-Glutamine E15-079 500 ml

Applications

> For cultivation of most cell types> Usage is recommended with supplementation of

10 % FBS

Features

> DMEM is a modification of the Eagle Medium with4.5 g/l glucose

> Contains a fourfold concentration of amino acids> Contains vitamins and additional components

Stable Glutamine

L-Glutamine is an essential amino acid and must beadded to cell culture media. Since L-glutamine is atemperature sensitive component DMEM is also availa-ble with the stabilized form of L-glutamine (L-alanyl-L-glutamine). Cells don't have to be adapted to DMEMcontaining Stable Glutamine.




Shelf Life . . . . . . . . . without L-Glutamine 18 months . . . . . . . . . . . . . .with Stable Glutamine 18 months

. . . . . . . . . . . . . . . . . . . . . with L-Glutamine 12 monthsStorage . . . . . . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous not in E15-078. . . . . . . . . . 200.00Ferric(III)-Nitrate • 9H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 97.70Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6400.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 125.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 3700.00

Amino AcidsL-Alanyl-L-Glutamine in E15-883. . . . . . . . . . . . . . . . . . . 876.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00L-Glutamine in E15-810, E15-843, E15-877 . . . . . . . . . . 584.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.00D-Valine in E15-055 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.00L-Valine not in E15-055. . . . . . . . . . . . . . . . . . . . . . . . . . 94.00

VitaminsD-Calcium-Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Choline Chloride. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.20Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.40Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00

Other ComponentsD-Glucose anhydrous not in E15-079 . . . . . . . . . . . . . . 4500.00Phenol Red not in E15-047, E15-877 . . . . . . . . . . . . . . . . 15.00Sodium Pyruvate in E15-011, E15-843 . . . . . . . . . . . . . 110.00

Dulbecco's MEMTS (DMEMTS), Thermostable Media


Med

ia

Cat. No. Volume

DMEMTS, low Glucose (1 g/l)without L-Glutamine E15-080 500 ml

DMEMTS, high Glucose (4.5 g/l)without L-Glutamine E15-082 500 ml

General

Liquid media consist of heat sensitive vitamins andminerals which traditionally must be stored in a tem-perature controlled environment of between +2°Cand +8°C. Thanks to a new and unique productionprocess it is no longer necessary to refrigerateThermostable Media and even better, they can be keptat room temperature for long periods of time.

Applications

> Supports growth of most cell lines> Liver cells (mouse), endothelial cells> 10 % FBS concentration recommended

Features

> Suitable for storage at room temperature> Stable for 12 months> Tested on most cell lines> Without chemical additives> Validated by extensive stability studies

Manufacturing

> PAA uses only highest purity raw materials> Innovative treatment of single components> Fractionated cryo treatment based on shock freezing

and graduated thawing


CO2-Concentration, optimum . . . . . . . . . . 8.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 350 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/ml

Cell Culture . . . . . . . . . . . . . . . . . . . . . . . . testedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . 12 monthsStorage . . . . . . . . . . . . . . . . . . . . +2°C to +22°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Ferric(III)-Nitrate • 9H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 97.70Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6400.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 125.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 3700.00

Amino AcidsL-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.00


Other ComponentsD-Glucose anhydrous in E15-080 . . . . . . . . . . . . . . . . . 1000.00D-Glucose anhydrous in E15-082 . . . . . . . . . . . . . . . . . 4500.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00

DMEM / Ham's F-12 Medium (1:1)


Med

ia

Cat. No. Volume

DMEM / Ham's F-12without L-Glutamine E15-012 500 ml

DMEM / Ham's F-12with L-Glutamine E15-813 500 ml

Applications

> Basic media for producing many serum free media> Supports media which are used for the production of

human proteins, segregated into the supernatant> Supports the growth of most cell lines> Suitable for pancreas cells and sertoli cells

Feature

1:1 mixture of a nutrient enriched Ham´s F-12 Mediumand a vitamin enriched DMEM Medium





Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 116.60Ferric(III)-Nitrate • 9H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.05Ferric(II)-Sulphate • 7H2O . . . . . . . . . . . . . . . . . . . . . . . . 0.417Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 311.80Cupric(II)-Sulphate • 5H2O. . . . . . . . . . . . . . . . . . . . . . . 0.0013Magnesium Chloride • 6H2O. . . . . . . . . . . . . . . . . . . . . . 61.20Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 48.84Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6996.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . . 62.50Di-Sodium Dihydrogen Phosphate anhydrous . . . . . . . . . . 71.02Zinc Sulphate • 7H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.432Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 1200.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.45L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147.50L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.50L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.65L-Cystine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . 31.29L-Cysteine • 2HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17.56L-Glutamic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.35L-Glutamine in E15-813 . . . . . . . . . . . . . . . . . . . . . . . . 365.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18.75L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 31.48L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54.47L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59.05L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91.25L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17.24L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35.48L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17.25L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26.25L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53.45L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9.02L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38.70L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.85

VitaminsD(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0035D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 2.24Choline Chloride. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.98Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.65Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12.60Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.02Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.00Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.031Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.219Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.17Thymidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.365Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.68

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 3151.00Hypoxanthine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.10DL-68-Lipoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.105Linoleic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.042Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.10Putrescine • 2HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.081Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55.00


Med

ia

Ham's F-10 Medium

Cat. No. Volume

Ham's F-10without L-Glutamine E15-014 500 ml

Ham's F-10with L-Glutamine E15-815 500 ml

General

Ham's F-10 Medium was originally developed to sup-port the growth of Chinese Hamster Ovary cells (CHO)and their recombinant derivatives, HeLa- and murine L-cells.

Applications

> Used with or without serum supplementation,depending on the cell type

> Human diploid cells, white blood cells (chromosomalanalysis)

> Primary cells from rat, rabbit or chicken




Shelf Life . . . . . . . . . without L-Glutamine 18 months . . . . . . . . . . . . . . . . . . . . . with L-Glutamine 12 months Storage . . . . . . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . . 33.29Ferric(II)-Sulphate • 7H2O . . . . . . . . . . . . . . . . . . . . . . . . 0.834Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285.00Potassium Dihydrogen Phosphate . . . . . . . . . . . . . . . . . . 83.00Cupric(II)-Sulphate • 5H2O. . . . . . . . . . . . . . . . . . . . . . . 0.0025Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 74.62Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7400.00Di-Sodium Hydrogen Phosphate. . . . . . . . . . . . . . . . . . . 153.70Zinc Sulphate • 7H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.029Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 1200.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211.00L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13.30L-Cysteine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 35.12L-Glutamic Acid anhydrous . . . . . . . . . . . . . . . . . . . . . . . 14.70L-Glutamine in E15-815 . . . . . . . . . . . . . . . . . . . . . . . . 146.20Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.51L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 21.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.60L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13.10L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29.30L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.48L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.96L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.50L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.50L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.57L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.60L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.81L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.50

VitaminsD(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.024D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . 0.715Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.698Folic Acide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.32Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.541Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.615Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.21Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.376Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.01Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.36

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 1100.00Hypoxanthine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.08Lipoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.21Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.20Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00Thymidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.73


Med

ia

Ham's F-12 Medium

Cat. No. Volume

Ham's F-12without L-Glutamine E15-016 500 ml

Ham's F-12with L-Glutamine E15-817 500 ml

Applications

> Serum free growth of Chinese Hamster Ovary cells(CHO)

> Cultivation of different kinds of mammalian cells(rat-hepatocytes, rat-prostate cells) with substitutionof serum

Features

Modification of the Ham´s F-10 Medium


CO2-Concentration, optimum . . . . . . . . . . 2.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 260 – 320 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 1EU/m


Shelf Life . . . . . . . without L-Glutamine 18 months . . . . . . . . . . . . . . . . . with L-Glutamine 12 months

Storage . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . . 33.29Ferric(II)-Sulphate • 7H2O . . . . . . . . . . . . . . . . . . . . . . . . 0.834Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223.60Cupric(II)-Sulphate • 5H2O. . . . . . . . . . . . . . . . . . . . . . . . 0.003Magnesium Chloride • 6H2O . . . . . . . . . . . . . . . . . . . . . 122.00Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7599.00di-Sodium Hydrogen Phosphate . . . . . . . . . . . . . . . . . . 142.04Zinc Sulphate • 7H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.863Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 1176.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.91L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210.70L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13.31L-Cysteine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 35.12L-Glutamic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14.70L-Glutamine in E15-817 . . . . . . . . . . . . . . . . . . . . . . . . 146.20Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.50L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 20.96L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.94L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13.10L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36.54L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.48L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.96L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34.53L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.50L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.90L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.04L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.44L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.70

VitaminsD(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.007D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 0.48Choline Chloride. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13.96Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.32Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18.02Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.037Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.062Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.038Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.34Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.36

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 1802.00Hypoxanthine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.08Linoleic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.084DL-α-Lipoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.21Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.10Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.20Putrescine • 2HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.161Deoxythymidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.73


Med

ia

Iscove's modified DMEM (IMDM)

Cat. No. Volume

IMDMwith 25mM HEPESwithout L-Glutamine E15-018 500 ml

IMDMwith 25mM HEPESwith L-Glutamine E15-819 500 ml

Applications

> Supports B-lymphocytes, bone marrow tissue andwith lipopolysaccharide stimulated B-cells

> Suitable for T-lymphocytes and most hybrid cells> Macrophage precursor cells> Erythrocyte precursor cells

Features

> Modification of the Dulbecco´s Modified Eagle´sMedium

> Contains not only selenium but also additionalamino acids, vitamins, sodium pyruvate and HEPESbuffer





Formulation mg/l

Inorganic SaltsCalcium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330.00Potassium Nitrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.076Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 97.67Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4505.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 125.00Sodium Selenite • 5H2O . . . . . . . . . . . . . . . . . . . . . . . . . 0.017Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 3024.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28.40L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Cysteine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70.00L-Glutamic Acid anhydrous . . . . . . . . . . . . . . . . . . . . . . . 75.00L-Glutamine in E15-819 . . . . . . . . . . . . . . . . . . . . . . . . 584.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74.86L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.00

VitaminsD(+)-Biotin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0130D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.20Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.40Thiamine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.013

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 4500.00HEPES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5958.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00


Med

ia

L-15 Medium (Leibovitz's)

Cat. No. Volume

L-15 Mediumwithout L-Glutamine E15-020 500 ml

L-15 Mediumwith L-Glutamine E15-821 500 ml

Applications

> Suitable for established cell lines, for example Hep-2,monkey kidney cells

> Qualified for primary explants from embryonic andadult human tissue

> Cultivation of many viruses> Cultivation of neuronal cells

Features

> Contains no sodium hydrogen carbonate> Supports the cell growth in CO2-free systems> Buffered by the supplementation of salts, free basic

amino acids and galactose


CO2-Concentration, optimum . . . . . . . . . . . . . 0.0 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . . . . 280 – 340 mOsmol/kg Endotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/ml



Formulation mg/l

Inorganic SaltsCalcium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Potassium Dihydrogen Phosphate . . . . . . . . . . . . . . . . . . 60.00Magnesium Chloride • 6H2O . . . . . . . . . . . . . . . . . . . . . 200.00Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 97.67Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8000.00Di-Sodium Hydrogen Phosphate. . . . . . . . . . . . . . . . . . . 190.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 225.00L-Arginine Base. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 500.00L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . 250.00L-Cysteine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120.00L-Glutamine in E15-821 . . . . . . . . . . . . . . . . . . . . . . . . 300.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200.00L-Histidine Base. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 250.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93.75L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 300.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 300.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100.00

VitaminsDL-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Riboflavin-5'-Phosphate Sodium Salt • H2O . . . . . . . . . . . . 0.10Thiamine Monophosphate Chloride . . . . . . . . . . . . . . . . . . 1.00

Other ComponentsD-Galactose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 900.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 550.00


Med

ia

Medium 199 with Earle's Salts

Cat. No. Volume

MEM 199 with Earle's Saltswithout L-Glutamine E15-033 500 ml

MEM 199 with Earle's Saltswith L-Glutamine E15-834 500 ml

Applications

> Versatile medium> Cultivation of non-transformed cells> Frequently used in virology and vaccine production> In vitro cultivation of primary explants of epithelial

mouse pancreas cells and rat tissues> Endothelial cells

Feature

Originally developed for nutritional studies of chickenembryos





Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Ferric(III)-Nitrate • 9H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.72Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 97.67Sodium Acetate • 3H2O . . . . . . . . . . . . . . . . . . . . . . . . . 82.95Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6800.00Sodium Dihydrogen Phosphate . . . . . . . . . . . . . . . . . . . 122.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2200.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Cysteine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Glutamic Acid anhydrous . . . . . . . . . . . . . . . . . . . . . . . 67.00L-Glutamine in E15-834 . . . . . . . . . . . . . . . . . . . . . . . . 100.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 21.88Hydroxy-L-Proline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00

VitaminsP-Aminobenzoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.05Ascorbic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.05D(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01Calciferol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01Choline Chloride. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.50Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.05Menadione. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.010Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.025Nicotinic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.025Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.025Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.025Retinol Acetate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.14Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01DL-α-Tocopherol Phosphate Na . . . . . . . . . . . . . . . . . . . . 0.010

Other ComponentsAdenine Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00AMP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.24ATP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Cholesterol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Deoxyribose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.50D-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 1000.00Glutathione . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.05Guanine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.30Hypoxanthine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.30Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21.30D-Ribose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.50Thymine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.30Tween 80 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00Uracil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.30Xanthine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.34


Med

ia

Medium 199 with Hanks' Salts

Cat. No. Volume

Medium 199 with Hanks' Saltswith 25 mM HEPESwithout L-Glutamine E15-037 500 ml

Medium 199 with Hanks' Saltswith 25 mM HEPESwith L-Glutamine E15-838 500 ml

Applications

> Versatile medium> Cultivation from non-transformed cells> Frequently used in virology and vaccine production> In vitro cultivation from primary explants of epithelial

mouse pancreas cells and rat tissues> Endothelial cells

Feature

Originally developed for nutritional studies of chickenembryos





Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 140.00Ferric(III)-Nitrate • 9H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.72Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Potassium Dihydrogen Phosphate . . . . . . . . . . . . . . . . . . 60.00Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 97.78Sodium Acetate • 3H2O . . . . . . . . . . . . . . . . . . . . . . . . . 83.00Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8000.00Di-Sodium Hydrogen Phosphate . . . . . . . . . . . . . . . . . . . 47.68Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . . 350.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Arginine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Cysteine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.11L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Glutamic Acid anhydrous . . . . . . . . . . . . . . . . . . . . . . . 67.00L-Glutamine in E15-838 . . . . . . . . . . . . . . . . . . . . . . . . 100.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 21.88Hydroxy-L-Proline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00

VitaminsP-Aminobenzoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.05Ascorbic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.057D(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01Calciferol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01Choline Chloride. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.50Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.05Menadione. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.011Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.025Nicotinic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.025Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.025Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.025Retinol Acetate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.14Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01DL-α-Tocopherol Phosphate Na . . . . . . . . . . . . . . . . . . . . 0.010

Other ComponentsAdenine Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00AMP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.24ATP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Cholesterol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Deoxyribose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.50D-Glucose anhydrous. . . . . . . . . . . . . . . . . . . . . . . . . . 1000.00Glutathione . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.05Guanine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.30HEPES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5958.00Hypoxanthine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.30Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00D-Ribose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.50Thymine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.30Tween 80 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00Uracil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.30Xanthine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.34


Med

ia

MEM Alpha Modification

Cat. No. Volume

MEM Alpha Modificationwith L-Glutaminewithout Ribonucleosides E15-832 500 ml

MEM Alpha Modificationwith L-Glutaminewith Ribonucleosides E15-862 500 ml

Applications

> Designed to support a wide variety of mammaliancell types

> Suitable for the cultivation of many cells grown inmonolayers

Features

> Similar formulation to Eagle's Media> Contains higher amino acid concentrations




Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 monthsStorage . . . . . . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . 97.67Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6800.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 140.00Sodium Hydrogen Carbonate in E15-832 . . . . . . . . . . . 2200.00Sodium Hydrogen Carbonate in E15-862 . . . . . . . . . . . . 850.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126.64L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Cysteine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . 100.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24.00L-Glutamic Acid anhydrous . . . . . . . . . . . . . . . . . . . . . . . 75.00L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 292.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.40L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.40L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.47L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46.00

VitaminsL-Ascorbic Acid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45.00D(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00D-Pantothenic Acid (hemicalcium) . . . . . . . . . . . . . . . . . . . 1.00Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.33

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 1000.00HEPES in E15-862 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5958.00Lipoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00

RibonucleosidesAdenosin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Cytidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Guanosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Uridine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00

Deoxyribonucleosides2'-Deoxyadenosine • H2O . . . . . . . . . . . . . . . . . . . . . . . . 10.002'-Deoxycytidine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . 11.002'-Deoxyguanosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.002'-Deoxythymidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00


Med

ia

Minimal Essential Medium Eagle (MEM)

Cat. No. Volume

MEM with Earle's Salts without L-Glutamine E15-024 500 ml

MEM with Earle's Salts with L-Glutamine E15-825 500 ml

Application

> Cultivation of many cells grown in monolayers

Features

> Modification of the Eagle Medium> Contains a higher concentration of amino acids





Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 97.70Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6800.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 140.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2200.00

Amino AcidsL-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24.00L-Glutamine in E15-825 . . . . . . . . . . . . . . . . . . . . . . . . 292.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.50L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46.00

VitaminsD-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 1000.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.00


Med

ia

RPMI 1640

Cat. No. Volume

RPMI 1640 without L-Glutaminewith Phenol Red E15-039 500 ml

RPMI 1640with L-Glutaminewith Phenol Red E15-840 500 ml

RPMI 1640with Stable Glutaminewith Phenol Red E15-885 500 ml

RPMI 1640 without L-Glutaminewith 25 mM HEPESwith Phenol Red E15-041 500 ml

RPMI 1640 with L-Glutaminewith 25 mM HEPESwith Phenol Red E15-842 500 ml

RPMI 1640 without L-Glutaminewithout Phenol Red E15-048 500 ml

RPMI 1640 with L-Glutaminewithout Phenol Red E15-848 500 ml

Application

> Cultivation of human and animal lymphocytes

Features

> Enriched medium, based on the formula of RPMI 1630> Originally developed for suspension and monolayer

cultures from human leukaemia cells

Stable Glutamine

L-Glutamine is an essential amino acid and must beadded to cell culture media. Since L-glutamine is a tem-perature sensitive component RPMI 1640 is also availablewith the stabilized form of L-glutamine (L-alanyl-L-gluta-mine). Cells don't have to be adapted to RPMI 1640 con-taining Stable Glutamine.


CO2-Concentration, optimum . . . . . . . . . . . . . 4.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 260 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/ml


Shelf Life . . . . . . . . . without L-Glutamine 18 months . . . . . . . . . . . . . .with Stable Glutamine 18 months

. . . . . . . . . . . . . . . . . . . . . with L-Glutamine 12 monthsStorage . . . . . . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Formulation mg/l

Inorganic SaltsCalcium Nitrate • 4H2O. . . . . . . . . . . . . . . . . . . . . . . . . 100.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.80Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6000.00Di-Sodium Hydrogen Phosphate anhydrous . . . . . . . . . . 800.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2000.00

Amino AcidsL-Alanyl-L-Glutamine in E15-885. . . . . . . . . . . . . . . . . . . 445.90L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241.86L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Glutamic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Glutamine in E15-840, E15-842, E15-848 . . . . . . . . . . 300.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Histidine Base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Hydroxyproline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00

VitaminsP-Aminobenzoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00D(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00D-Pantothenic Acid (hemicalcium) . . . . . . . . . . . . . . . . . . . 0.25Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.005

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 2000.00Glutathione (red.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00HEPES in E15-041 and E15-842 . . . . . . . . . . . . . . . . . . 5957.50Phenol Red in E15-039, E15-840, E15-041, E15-842, E15-885 . . . . . . . . . . . . . . . . . . . . . . . 5.00


Med

ia

RPMI 1640TS, Thermostable Media

Cat. No. Volume

RPMI 1640TS

without L-Glutamine E15-084 500 ml

General

Liquid media consist of heat sensitive vitamins andminerals which traditionally must be stored in a tem-perature controlled environment of between +2°Cand +8°C. Thanks to a new and unique productionprocess it is no longer necessary to refrigerateThermostable Media and even better, they can be keptat room temperature for long periods of time.

Application

> Cultivation of human and animal lymphocytes

Features

> Suitable for storage at room temperature> Stable for 12 months> Tested on most cell lines> Without chemical additives> Validated through extensive stability studies

Manufacturing

> PAA uses only the highest purity raw materials> Innovative treatment of single components> Fractionated cryo treatment based on shock freezing

and graduated thawing




Shelf Life . . . . . . . . . . . . . . . . . . . . . . . 12 monthsStorage . . . . . . . . . . . . . . . . . . . . +2°C to +22°C

Formulation mg/l

Inorganic SaltsCalcium Nitrate • 4H2O. . . . . . . . . . . . . . . . . . . . . . . . . 100.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.80Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6000.00Di-Sodium Hydrogen Phosphate anhydrous . . . . . . . . . . 800.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2000.00

Amino AcidsL-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241.86L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Glutamic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Histidine Base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Hydroxyproline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00


Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 2000.00Glutathione (red.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.00


Med

ia

DMEM Ready Mix

Cat. No. Volume

DMEM Ready Mix R15-801 500 ml

Our Idea

> The concept: a ready to use complete medium > Suitable for all systems using DMEM medium

Application

> Cultivation of many monolayer cells

Advantages

> No additional pipetting necessary> Minimizes possible mistakes with additives > Reduces the risk of contamination > Always defined with FBS Gold> No batch to batch variations

Composition

DMEM Ready Mix consists of DMEM high glucose (4.5 g/l) supplemented with 10% FBS Gold and L-glu-tamine.


CO2-Concentration, optimum . . . . . . . . . . . . . 8.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . . < 20 EU/mlTotal Protein . . . . . . . . . . . . . . . . . . . . . . 0.3 – 0.6 g/dl

Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . PI-3, BVD-AB, BVD-V, BHV-ICell Culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . testedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . 3 months at +2°C to +8°C2 years at ≤ −15°C

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Ferric(III)-Nitrate • 9H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 97.70Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6400.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 122.84Sodium Hydrogen Carbonate . . . . . . . . . . . . . . . . . . . 3700.00

Amino AcidsL-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00L-Glutamine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 584.00Glycine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Phenylalanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Serine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.00

VitaminsCholine Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.20Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00D-Pantothenic Acid (hemicalcium). . . . . . . . . . . . . . . . . . . 4.00Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.40Thiamine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 4500.00Foetal Bovine Serum Gold. . . . . . . . . . . . . . . . . . . . . . . . 10 %Phenol Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00


Med

ia

RPMI 1640 Ready Mix

Cat. No. Volume

RPMI Ready Mix R15-802 500 ml

General

Our idea was to develop a ready to use completemedium suitable for all systems using RPMI 1640medium.

Application

> Cultivation of many monolayer cells

Advantages

> No additional pipetting necessary> Minimizes possible mistakes with additives > Reduces the risk of contamination > Always defined with FBS Gold> Batches are able to be constantly reproduced

Composition

RPMI 1640 Ready Mix consists of RPMI 1640 supple-mented with 10 % FBS Gold and L-glutamine.


CO2-Concentration, optimum . . . . . . . . . . 4.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 260 – 320 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 20 EU/mlTotal protein . . . . . . . . . . . . . . . . . . 0.3 – 0.6 g/dl

Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . . .PI-3, BVD-AB, BVD-V, BHV-ICell Culture . . . . . . . . . . . . . . . . . . . . . . . . testedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . 3 months at +2°C to +8°C2 years at ≤ −15°C

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Formulation mg/l

Inorganic SaltsCalcium Nitrate • 4H2O. . . . . . . . . . . . . . . . . . . . . . . . . 100.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.80Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6000.00Di-Sodium Hydrogen Phosphate anhydrous . . . . . . . . . . 800.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2 000.00

Amino AcidsL-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241.86L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Glutamic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 300.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Histidine Base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Hydroxyproline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00


Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 2000.00Foetal Bovine Serum Gold . . . . . . . . . . . . . . . . . . . . . . . . 10 %Glutathione (red.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.00


Med

iaQUANTUM 3-21Medium for Cultivation of Amnion and Chorionic Villi Cells

Cat. No. Volume

QUANTUM 3-21 U11-020 100 ml Medium for cultivation of amnion and chorionic villi cells

General

QUANTUM 3-21 has been specifically developed forthe prenatal diagnostic testing of human amnion andchorionic villi cells. The cultivation of these cells andthe preparation of karyograms allows the detection ofpossible chromosomal aberrations.

Applications

> Prenatal diagnostics> Selective growth of amnion and chorionic villi cells

Features

> Ready to use medium> Fast cell growth> No chromosomal breaks> Simple detection of chromosomal aberrations> Cell harvest after 7 - 10 days> Clear and rapid diagnostic results> Unlimited availability

For in vitro diagnostic use only

marked

With Quantum 3-21, PAA offers a CE marked mediumfor IVD which fulfils the requirements of the directive98/79/EC defined by the European Commission.


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Endotoxin . . . . . . . . . . . . . . . . . . . . . . . < 5 EU/ml

Amniotic fluid cell growth . . . . . . . . . . . . . passMycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . . PI-3, BVD-AB, BVD-V, BHV-ISterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . 24 monthsStorage . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . 97.67Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5500.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 140.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2200.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126.64L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Cysteine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . 100.00L-Cysteine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24.00L-Glutamic Acid anhydrous . . . . . . . . . . . . . . . . . . . . . . . 75.00L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 292.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.40L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.40L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.47L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36.20L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46.00

VitaminsL-Ascorbic Acid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00D(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.20Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.33

RibonucleosidesAdenosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Cytidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Guanosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Uridine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00

Deoxyribonucleosides2'-Deoxyadenosine • H2O . . . . . . . . . . . . . . . . . . . . . . . . 10.002'-Deoxycytidine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . 11.002'-Deoxyguanosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.002'-Deoxythymidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 2000.00Gentamycin Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00Lipoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00FBS amnion cell tested . . . . . . . . . . . . . . . . . . . . . . prop. info.*Hormones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Growth factors . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Other additives . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*

* proprietary information


Med

ia

QUANTUM PBLMedium for Cultivation of peripheral Blood Lymphocytes

Cat. No. Volume

QUANTUM PBL U11-022 100 ml Medium for cultivation of peripheral blood lymphocytes

General

Blood cell karyotyping of lymphocytes is an importanttool in modern human cytogenetics to detect chromo-somal aberrations. For cytogenetic analysis peripheralblood lymphocytes are cultivated in specific media andintact chromosomes are prepared. QUANTUM PBL is acomplete medium stimulating the growth of periphe-ral blood lymphocytes used for preparation of intactchromosomes. It contains the mitogen phytohaemag-glutinin (PHA) as growth determining factor.

Applications

> Postnatal diagnostics> Cultivation of peripheral blood lymphocytes> Detection of chromosomal aberrations

Features

> New formulation> Ready to use medium> Suitable for heparinized blood and lymphocyte

enriched blood> Faster cell growth> Optimized PHA concentration> High yield of intact chromosomes

Composition

The complete medium consists of a modified RPMI1640 medium, phytohaemagglutinin (PHA), FBS pre-tested on lymphocytes and penicillin/streptomycin asantibiotics.

For in vitro diagnostic use only

marked

With Quantum PBL, PAA offers a CE marked mediumfor IVD which fulfils the requirements of the directive98/79/EC defined by the European Commission.


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 − 7.5Osmolality . . . . . . . . . . . . 280 − 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 25 EU/ml

Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . . PI-3, BVD-AB, BVD-V, BHV-ISterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . . . . . . . . . . . . 24 monthsStorage . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Formulation mg/l

Inorganic SaltsCalcium Nitrate • 4H2O. . . . . . . . . . . . . . . . . . . . . . . . . 100.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate dried . . . . . . . . . . . . . . . . . . . . . . . 48.80Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5500.00Di-Sodium Hydrogen Phosphate. . . . . . . . . . . . . . . . . . . 800.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2000.00


VitaminsP-Aminobenzoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00D(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 0.25Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 2000.00Glutathione (red.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Penicillin/Streptomycin (P11-010) . . . . . . . . . . . . . . . . . . 10 ml/lPhenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.00Phytohaemagglutinin (J01-006) . . . . . . . . . . . . . . . . . . 100 ml/lPlant Extracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Foetal Bovine Serum. . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Hormones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

ia

Ham's F-10 Ready Mix

Cat. No. Volume

Ham's F-10 Ready Mix R11-821 100 ml

General

Karyotyping of lymphocytes, amnion and chorionic villicells is an important tool in cytogenetics to detect chro-mosomal aberrations. For chromosomal analysis lympho-cytes of the peripheral blood and cells of the amnionfluid are cultivated in specific media in order to prepareintact chromosomes. Ham´s F-10 is one of the mostwidely used culture media in cytogenetics. For optimaland reproducible growth of amnion and chorionic villicells it is supplemented with Foetal Bovine Serum (FBS) asit is an essential source of specific growth factors.After supplementation with phytohaemagglutinin (PHA)Ham´s F-10 Ready Mix is a complete medium for selecti-ve growth of lymphocytes of the peripheral blood.

Applications

> Optimized medium for amnion and chorionic villi cells> Qualified for growth of lymphocytes after stimu-

lation with PHA> Chromosomal analysis> Karyotyping> Fluorescence In Situ Hybridisation (FISH analysis)

Features

> Ready to use medium> Reduced chances of contamination > Amnion cell tested FBS> Adapted for amnion and chorionic villi cells as well

as lymphocytes > Optimized reproducibility

Composition

> Ham's F-10 > FBS, amnion and chorionic villi cell tested> Gentamicin> L-Glutamine

PHA has to be added for stimulation of lymphocytes!


CO2-Concentration, optimum . . . . . . . . . . 2.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 20 EU/mlTotal Protein . . . . . . . . . . . . . . . . . . 0.6 – 1.2 g/dl

Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . PI-3, BVD-AB, BVD-V, BHV-ICell Culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . testedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . 2 years Storage . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . . 33.29Ferric(II)-Sulphate • 7H2O . . . . . . . . . . . . . . . . . . . . . . . . 0.834Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285.00Potassium Dihydrogen Phosphate . . . . . . . . . . . . . . . . . . 83.00Cupric(II)-Sulphate • 5H2O. . . . . . . . . . . . . . . . . . . . . . . 0.0025Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 74.62Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7400.00Di-Sodium Hydrogen Phosphate. . . . . . . . . . . . . . . . . . . 153.70Zinc Sulphate • 7H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.029Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 1200.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211.00L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13.30L-Cysteine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 35.12L-Glutamic Acid anhydrous . . . . . . . . . . . . . . . . . . . . . . . 14.70L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.20Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.51L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 21.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.60L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13.10L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29.30L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.48L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.96L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.50L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.50L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.57L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.60L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.81L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.50

VitaminsBiotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.024Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.698Folic Acide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.32Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.541Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.615D-Pantothenic Acid (hemicalcium) . . . . . . . . . . . . . . . . . . 0.715Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.21Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.376Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.01Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.36

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 1100.00FBS amnion and chorionic villi cell tested . . . . . . . . . . . . . . 20 %Gentamicin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00Hypoxanthine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.08Lipoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.21Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.20Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00Thymidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.73


Med

ia

Neuronal Base Medium

Cat. No. Volume

Neuronal Base Mediumwithout L-Glutamine U15-023 500 ml

General

PAA`s Neuronal Base Medium is formulated to meetthe special requirements of neuronal cells. In combina-tion with different NeuroMixes (B27 NeuroMix,Neuronal Stem Cell Supplement) the completemedium allows growth of neuronal cells without theneed of an astrocyte feeder layer. The basal mediumcontains vitamins, trace elements and amino acidswhich are also optimized for growth of neuronaltumour cell lines.

Applications

> Serum free cell culture of neuronal cells > In combination with different NeuroMixes> For neuronal tumour cell lines

Features

> Long term growth of foetal hippocampal neurons> Long term growth of different neurons of the central

nervous system (CNS) and peripheral nervous system(PNS)

> Contains a range of different antioxidants> Reduction of reactive oxygen damage



Cell Culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . testedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . 18 monthsStorage . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Ferric(III)-Nitrate • 9H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Chloride anhydrous. . . . . . . . . . . . . . . . . . . . 77.30Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3000.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 125.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2200.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.83L-Cysteine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.21Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.76L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.00

VitaminsCholine Chloride. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.20Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00D-Pantothenic Acid (hemicalcium) . . . . . . . . . . . . . . . . . . . 4.00Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.40Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.34

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 4500.00HEPES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2600.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.10Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00Trace Elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Other Additives . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

ia

Neuronal Base Medium AD

Cat. No. Volume

Neuronal Base Medium ADwithout L-Glutamine U15-024 500 ml

General

PAA`s Neuronal Base Medium AD is formulated tomeet the special requirements of adult and postnatalneurons. In combination with different NeuroMixes(B27 NeuroMix, Neuronal Stem Cell Supplement) thesupplemented medium allows growth of adult neuro-nal cells without the need of an astrocyte feeder layer.

Applications

> Serum free cell culture of adult neuronal cells > In combination with different NeuroMixes

Features

> Long term growth of rat postnatal hippocampal neu-rons and other adult mammalian neurons

> Long term growth of different adult neurons of thecentral nervous system (CNS) and peripheral nervoussystem (PNS)


CO2-Concentration, optimum . . . . . . . . . . 5.0 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . 250 – 270 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/ml



Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Ferric(III)-Nitrate • 9H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Chloride anhydrous. . . . . . . . . . . . . . . . . . . . 77.30Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3730.50Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 125.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2200.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.83L-Cysteine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.21Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.76L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.00

VitaminsCholine Chloride. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.20Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00D-Pantothenic Acid (hemicalcium) . . . . . . . . . . . . . . . . . . . 4.00Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.40Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.34

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 4500.00HEPES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2600.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.10Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00Trace Elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

ia

CollagenStem Kit

Cat. No. Volume

CollagenStem Kit U115-033CollagenStem Medium 100 mlCollagenStem Solution (3.3 mg/ml) 50 ml

General

Collagen based media are used for the cultivation ofhuman or murine hematopoietic progenitor cells.Collagen, a gelling agent, increases the viscosity of themedium allowing optimal colony formation of the dif-ferent progenitor cells for simplified separation andidentification. CollagenStem Medium (containingCollagenStem Solution) is used for the cultivation ofcells which have to be dehydrated and fixed for subse-quent cytochemical or immuno-cytochemical staining.

Applications

> Detection and quantification of hematopoietic pro-genitor cells after addition of different cytokines

> Cytochemical or immuno-cytochemical staining

Features

> Serum free basal medium> Good optical clarity> Easy visualisation> Formation and isolation of distinct colonies

Composition

CollagenStem Medium consists of a modified IMDMmedium containing BSA, 2-Mercaptoethanol and L-glutamine. Before use the CollagenStem Solutionhas to be added to the medium.

Note: FBS (10-30%) and cytokines can be added forsome applications.

Product Specifications Medium

CO2-Concentration, optimum . . . . . . . . . . 7.0 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 350 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . value

Sterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life Medium . . . . . . . . . . . . . 24 monthsSolution . . . . . . . . . . . . . 12 months

Storage Medium . . . . . . . . . . . . . . ≤ −15°CSolution . . . . . . . . . . +2°C to +8°C

Formulation Medium mg/l


Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28.40L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Cysteine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70.00L-Glutamic Acid anhydrous . . . . . . . . . . . . . . . . . . . . . . . 75.00L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 584.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74.86L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.00

VitaminsD(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0130D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.20Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.40Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.013

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 4500.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00HEPES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5958.00Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00BSA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*2-Mercaptoethanol . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

ia

MethoStem Medium

Cat. No. Volume

MethoStem Mediumwith L-Glutamine U11-827 100 ml

General

Methylcellulose based media are used for the culti-vation of hematopoietic and embryonic stem cells andtheir progenitors. As a gelling agent methylcelluloseincreases the viscosity of the medium. Addition ofmethylcellulose promotes optimal colony formation ofthe different progenitor cells for simplified separationand identification. For differentiation of the stem cellsfurther cytokines must be added.

Applications

> Growth and differentiation of hematopoietic stem cells

> Growth of embryonic stem cells> Differentiation and characterization of stem

cell progenitors

Features

> Aqueous gelling agent> Good optical clarity> Easy visualisation> Formation and isolation of distinct colonies

Composition

MethoStem Medium consists of a modified IMDMmedium containing 1.8 % methylcellulose, BSA, 2-Mercaptoethanol and L-glutamine.MethoStem is a basal medium where additional com-ponents, such as FBS (10-30%), cytokines and addi-tional IMDM, must be added to get a final concentra-tion of approximately 0.9% methylcellulose.


CO2-Concentration, optimum . . . . . . . . . . 7.0 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 350 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . value


Shelf Life . . . . . . . . . . . . . . . . . . . . . . . 24 months Storage . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Formulation mg/l


Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28.40L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Cysteine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70.00L-Glutamic Acid anhydrous . . . . . . . . . . . . . . . . . . . . . . . 75.00L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 584.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74.86L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.00

VitaminsD(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0130D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Choline Chloride. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.20Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.40Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.013

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 4500.00HEPES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5958.00Methylcellulose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18000.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00BSA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*2-Mercaptoethanol . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

ia

MesenchymStem Medium

Cat. No. Volume

MesenchymStem Mediumwith L-Glutamine U15-828 500 ml

General

Mesenchymal stem cells, part of the hematopoieticstem cell network, have become a very important partof research into human therapeutics. Mesenchymal stem cells, derived from bone marrow,are capable of self renewal and of differentiation intobone-, cartilage-, muscle- and fat cells. Differentiationinto distinct cell types can be achieved by directed sti-mulation using cytokines. These cells have many valu-able applications for example in regenerative medicinefor reimplantation into patients.

Applications

> Growth of mesenchymal stem cells> Growth of mesenchymal progenitor cells> Preparation of mesenchymal cells for differentiation

Features

> Optimized formulation for expansion of mesenchy-mal cells

> Simplified characterization and quantification ofmesenchymal progenitors

Composition

MesenchymStem Medium consists of a modifiedDMEM medium containing specific trace elements,FBS and L-glutamine.

Note: For differentiation of mesenchymal cells intobone-, cartilage-, muscle- and fat cells cytokines mustbe added.


CO2-Concentration, optimum . . . . . . . . . . 8.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 350 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 20 EU/ml



Formulation mg/l


Amino AcidsL-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 584.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.00

VitaminsCholine Chloride. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.20Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00D-Pantothenic Acid (hemicalcium) . . . . . . . . . . . . . . . . . . . 4.00Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.40Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.00

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 4500.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00Trace Elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Foetal Bovine Serum. . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

ia

Endothelial Cell Medium

Cat. No. Volume

Endothelial Cell Medium without L-Glutamine U15-002 500 ml

Endothelial Basal MediumMCDB 131 U15-011 500 ml

Endothelial Medium Supplementwith growth factors and heparin without antibiotics U05-012 50 ml

General

As a model system of the vascular system, endothelialcells are cultivated in vitro. The segregation of growthfactors and the maintaining of the selective permeableanti-thrombogen barrier, as well as the metabolism oflipids, are important factors for the interpretation ofthe complete system.Serum free cultivation with PAA's Endothelial CellMedium avoids toxic side effects and high endotoxinlevels. Defined composition and the absence of serumspecific batch variations provides reliable results.

Features

> Serum free defined medium> Ready to use> Optimized for the cultivation of umbilical arterial and

dermal micro-vascular endothelial cells

Advantages

> No batch to batch variation> High level of reproducibility> No contamination risk> Low endotoxin level> No influence on the endothelial cell marker> Individual components available

The endothelial cell medium is not only available ascomplete medium but also as a basal medium plus theindividual components as supplement.


CO2-Concentration, optimum . . . . . . . . . . 5.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality U15-002 . . . . . . . 260 – 320 mOsmol/kg

U15-011, U05-012 280 – 340 mOsmol/kgEndotoxin U15-002 . . . . . . . . . . . . . . . < 1 EU/ml

U15-011, U05-012 . . . . . . . . . < 10 EU/ml

Sterility . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life U15-011 . . . . . . . . . . . . . . 18 monthsU15-002 . . . . . . . . . . . . . . 24 monthsU05-012 . . . . . . . . . . . . . . . 36 months

Storage U15-011 . . . . . . . . . . . . +2°C to +8°CU15-002, U05-012 . . . . . . . . . ≤ −15°C

Formulation mg/l

Inorganic SaltsAmmonium Metavanadate . . . . . . . . . . . . . . . . . . . . . . 0.0006Ammonium Molybdate • 4H2O . . . . . . . . . . . . . . . . . . . 0.0037Calcium Chloride • 2H2O . . . . . . . . . . . . . . . . . . . . . . . 235.00Cupric Sulphate • 5H2O . . . . . . . . . . . . . . . . . . . . . . . . 0.0012Ferric Sulphate • 7H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.278Magnesium Sulphate • 7H2O. . . . . . . . . . . . . . . . . . . . 2465.00Manganese Sulphate • H2O. . . . . . . . . . . . . . . . . . . . . . 0.0002Nickelous Chloride • 6H2O . . . . . . . . . . . . . . . . . . . . . . 0.0001Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . 298.00Selenious Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0044Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6430.00Sodium Hydrogen Carbonate . . . . . . . . . . . . . . . . . . . 1176.00Sodium Meta Silicate • 9H2O. . . . . . . . . . . . . . . . . . . . . . . 2.80Sodium Phosphate dibasic • 7H2O . . . . . . . . . . . . . . . . . 134.00Zinc Sulphate • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 0.00019

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.67L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63.21L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.01L-Aspartic acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13.31L-Cysteine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 35.12L-Glutamic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.41Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.25L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 41.92L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65.60L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131.20L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182.60L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14.92L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33.04L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.51L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31.53L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.91L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.08L-Tyrosine 2Na • 2H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 22.52L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117.10

VitaminsBiotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0073Choline chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13.96D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . 12.92Folinic Acid Calcium Salt . . . . . . . . . . . . . . . . . . . . . . . . 0.5115I-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.21Niacinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.11Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.05Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0038Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.37Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0136

Other ComponentsAdenine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.1716D-Glucose (Dextrose) . . . . . . . . . . . . . . . . . . . . . . . . . . 1000.00Lipoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0021Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12.42Putrescine • 2HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0002Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00Thymidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.0242Albumine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Fibronectin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Heparin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Immunglobulin . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

ia

Macrophage Medium

Cat. No. Volume

Macrophage Medium murinewithout L-Glutamine U15-005 500 ml

General

Macrophages and monocytes play a major role duringimmunological reactions in organisms.They modulate and enhance the immunological res-ponse during defence of foreign organisms such asbacteria or viruses. They are also involved duringinflammation processes, in the resorption of cells andtissues and during reparation of tissue and skin.Macrophages and monocytes are an important ex vivomodel system for immunological processes.

In the past media containing serum were used for thecultivation of these cells; however the use of serum isrisk-bearing and diminishes the reproducibility.

PAA has developed a macrophage medium, which isfully defined and absolutely safe in use.

Features

> Serum free defined medium> For cultivation of mouse cells

Advantages

> Reproducible results> Low endotoxin level (< 1 EU/ml)> Supports macrophages as well as monocytes> No unspecific stimulation> No batch to batch variations


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/ml



Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . 97.67Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6800.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 140.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2200.00

Amino AcidsL-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126.64L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30.00L-Cysteine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . 100.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24.00L-Glutamic Acid anhydrous . . . . . . . . . . . . . . . . . . . . . . . 75.00L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 292.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.40L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.40L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.47L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32.00L-Proline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46.00

VitaminsL-Ascorbic Acid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45.00D(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.33

RibonucleosidesAdenosin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Cytidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Guanosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Uridine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00

Deoxyribonucleosides2'-Deoxyadenosine • H2O. . . . . . . . . . . . . . . . . . . . . . . . . 10.002'-Deoxycytidine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.002'-Deoxyguanosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.002'-Deoxythymidine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 1000.00Lipoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00Bovine and murine Proteins . . . . . . . . . . . . . . . . . . prop. info.*Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

ia

Hybridoma Express

Cat. No. Volume

Hybridoma Expresswithout L-Glutamine U15-001 500 ml

General

Hybridoma cells are usually cultivated in unspecificmedia, for example RPMI, DMEM or DMEM/Ham's F-12, with the addition of 10% Foetal Bovine Serum.The use of serum induces a number of concerns suchas biological contamination risks and increased down-stream purification complexity.Hybridoma Express is a fully defined serum freemedium for hybridoma cells. The low protein contentsimplifies the purification of the produced antibodieswithout loss of the activity of the proteins.

Features

> Low protein content (11µg/ml)> Chemically defined> Supports hybridoma cells> High growth capability for lymphoid and

epithelial cells> With Pluronic F68 for minimizing shear forces

in fermenters

Advantages

> Highest production efficiency> Highest expression rate > Suitable for use in fermenters> Contains no animal derived by-products> May be used for static cells


CO2-Concentration, optimum . . . . . . 5.0 – 8.0 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/ml



Note:Hybridoma Express is light sensitive. The mediumshould be protected from light during shipping andstorage.


Med

ia

InsectExpress Sf9-S2

Cat. No. Volume

InsectExpress Sf9-S2with L-Glutamine E15-875 500 ml

General

The baculovirus expression system is the most effecti-ve expression system of eukaryotic genes. Protein titresof more than 500 mg/l are achievable.Serum free media are free from batch to batch varia-tion of serum. Protein free media additionally show theadvantage of easy purification of the expressed pro-tein.PAA offers with its Insect Express Sf9-S2 a protein freemedium, which is optimized for the cultivation ofSpodoptera frugiperda and Drosophila S2 cells.

Applications

> Optimized for cultivation of Spodoptera frugiperdaand Schneider´s S2 drosophila cells

> Developed for the baculovirus expression systemwith BEVS

> Suitable for adherent as well as suspension cells inbioreactors

Features

> Protein free complete medium> Fully defined> Growth densities higher than 107 cells/ml> High glucose content> With Pluronic F68 for minimizing shear forces> Without hypoxanthine and thymidine> Hydrolysate source from yeast> Without HEPES> Without phenol red

Advantages

> Proteins will be modified posttranslationally> Cytoplasmatic as well as cell surface proteins can be

expressed> High specific productivity> No adaptation from cell cultures containing serum

necessary> No feeding necessary for 10 days> Increased yield> Suitable as freezing medium

Packaging Sizes

For application in larger units (fermenters) the InsectExpress Sf9-S2 can also be offered in customized sizes.Adapters, connectors and volumes can be individuallyconfigured.




Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . 9 monthsStorage . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Note:InsectExpress Sf9-S2 is light sensitive. The mediumshould be protected from light during shipping andstorage.


Med

ia

TC 100 Insect Medium

Cat. No. Volume

TC 100 Insect Mediumwith L-Glutamine E15-856 500 ml

Applications

> Supports the growth of Sf9-cells> Suitable for various lepidoptera cells> Suitable for baculovirus expressions

Features

> Modification of Grace's medium> Fully defined composition> Suitable for insect cells and virus production> Tested on insect cell cultures


CO2-Concentration, optimum . . . . . . . . . . 0.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.2 – 6.6Osmolality . . . . . . . . . . . . . . 200 – 300 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/ml

Sterility . . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . 12 monthsStorage . . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Formulation mg/l

Inorganic SaltsCalcium Chloride • 2H2O . . . . . . . . . . . . . . . . . . . . . . . 1298.11Magnesium Chloride • 6H2O . . . . . . . . . . . . . . . . . . . . 2282.59Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . 1781.00Potassium Chloride. . . . . . . . . . . . . . . . . . . . . . . . . . . . 2900.00Sodium Dihydrogen Phosphate • 9H2O . . . . . . . . . . . . . . 970.00

Amino AcidsL-Alanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 225.00L-Arginine Base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 550.00L-Asparagine • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391.97L-Asparic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20.00L-Glutamine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 600.00L-Glutamic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 650.00Glycine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 650.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . 3400.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Leucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 630.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50.00L-Phenylalanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150.00L-Proline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350.00L-Serine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 550.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100.00

Vitaminsp-Aminobenzoic Acid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.02D(+)-Biotin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 0.11Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.02Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.02Nicotine Acid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.02Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.02Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.02Thiamine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.02Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.01

Other ComponentsD(+)-Glucose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1000.00Bacto-Tryptose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2600.00

Note:TC 100 Insect Medium is light sensitive. It should beprotected from light during shipping and storage.


Med

ia

QUANTUM 101Complete Medium for HeLa Cells

Cat. No. Volume

QUANTUM 101with L-Glutamine U15-814 500 ml

General

HeLa cells are usually grown in RPMI 1640 medium,supplemented only with serum. For optimal growthadditional enhancers, trace elements and cofactors areneeded.Quantum 101 is a new formulation of the basalmedium RPMI 1640 with specific serum componentspurified by chromatography. Additionally Quantum101 is supplemented with selected components ofyeast and soybean extract.

Features

> Optimized balanced mixture of trace elements> Supplemented with protein components from yeast

extract> Supplemented with protein fractions from soybean> Supplemented with selected serum components > With L-glutamine

Advantages

> Ready to use> Optimized for HeLa cells> Defined culture conditions> No serum addition necessary> Reproducible working conditions> Minimizes the risk of contamination

Composition

Modified formulation based on RPMI 1640 with a newmixture of trace elements, vitamins and cofactors.




Shelf Life . . . . . . . . . . . 3 months at +2°C to +8°C . . . . . . . . . . . . . . . . . . . . . . . . . . 2 years ≤ −15°C

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Formulation mg/l

Inorganic SaltsCalcium Nitrate • 4H2O. . . . . . . . . . . . . . . . . . . . . . . . . 100.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.80Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6000.00Di-Sodium Hydrogen Phosphate. . . . . . . . . . . . . . . . . . . 800.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2000.00


VitaminsP-Aminobenzoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00D(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 0.25Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.005

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 2300.00Glutathione (red.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.00Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Soybean Extract . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Trace Elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Yeast Extract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

ia QUANTUM 263 Complete Medium for Tumour Cells

Cat. No. Volume

QUANTUM 263 with L-Glutamine U15-815 500 ml

General

Tumour cells have several advantages for long-termcultivation because they are potentially immortal.Tumour cells are ideal as a model for the investigationof the cell cycle and regulation on a molecular biolo-gical level.Quantum 263 is a newly formulated basal mediumwith an optimized mixture of trace elements and selec-ted serum components. Added to this is an importantsynthetic iron-binding molecule which facilitates thetransport of iron ions into the cell.

Features

> New balanced mixture of trace elements with speci-fic growth factors

> Selected protein extracts from yeast> Supplemented with a synthetic iron-binding molecu-

le for better uptake of iron ions> Supplemented with selected fractions of serum com-

ponents

Advantages

> Ready to use> Optimized for tumour cells> Defined culture conditions> No serum addition necessary> Reproducible working conditions> Minimizes the risk of contamination

Composition

Modified formulation based on medium RPMI 1640with an optimized mixture of trace elements.


CO2-Concentration, optimum . . . . . . . . . . . . . 4.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . . . < 25 EU/ml

Cell Culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . testedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . . . . 3 months at +2°C to +8°C . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 years at ≤ −15°C

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Formulation mg/l

Inorganic SaltsCalcium Nitrate • 4H2O . . . . . . . . . . . . . . . . . . . . . . . . . 100.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate dried . . . . . . . . . . . . . . . . . . . . . . . . 48.80Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6000.00Di-Sodium Hydrogen Phosphate . . . . . . . . . . . . . . . . . . . 800.00Sodium Hydrogen Carbonate . . . . . . . . . . . . . . . . . . . . 2000.00


VitaminsP-Aminobenzoic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00D(+)-Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20D-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 0.25Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Pyridoxine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.20Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Vitamin B12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.005

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 2700.00Glutathione (red.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.00Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Soybean Extract . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Synthetic iron binding Molecule . . . . . . . . . . . . . . . prop. info.*Trace Elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Yeast Extract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

ia

QUANTUM 286Complete Medium for Epithelial Cells

Cat. No. Volume


General

Epithelial cells and tissue play an important role in thecontrol of resorption and secretion plus the uptake ofstimuli within specialized sensory cells. Normally epithe-lial cells have been cultivated in medium containingserum, but some factors tend to inhibit epithelial proli-feration. Consequently, one of the most significantdevelopments in isolation and propagation of epithelialcells is the use of specific basal media containing selec-ted serum components like specific growth factors.Quantum 286 is a newly formulated base mediumwith a balanced mixture of trace elements and selec-ted serum components supplemented with specificprotein fractions of yeast and soybean extract.

Features

> New balanced mixture of trace elements> With selected protein fractions from yeast> Supplemented with protein components of soybean

extract> Supplemented with selected serum components

with growth enhancers

Advantages

> Ready to use> Optimized for epithelial cells> Defined culture conditions> No addition of serum necessary> Reproducible working conditions> Minimizes the risk of contamination

Composition

Modified formulation based on DMEM with a newbalanced mixture of trace elements.




Shelf Life . . . . . . . . . . . 3 months at +2°C to +8°C . . . . . . . . . . . . . . . . . . . . . . . . 2 years at ≤ −15°C

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Formulation mg/l


Amino AcidsL-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 584.00Glycine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98.00


Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . . 2700.0Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110.00Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Protein Components . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Soybean Extract . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Trace Elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Yeast Extract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Med

iaQUANTUM 333Complete Medium for Fibroblasts

Cat. No. Volume


General

Fibroblasts are young cells derived from the mesenchy-me. They are involved in formation of the intercellularsubstance of the connective tissue and fibres.Fibroblasts play an important role within research intothe recovery of destroyed tissues. Quantum 333 is a newly formulated basal mediumwith a balanced mixture of trace elements, selectedserum fractions supplemented with insulin and aneffective iron-binding molecule. The growth enhancersof the medium interact perfectly with the growth fac-tors of the serum components for ideal culture condi-tions of fibroblasts. The complete medium enables the cultivation of pri-mary cells as well as the maintenance of normal celllines. The medium supports optimal growth of cellsfrom human and animal origin.

Features

> New balanced mixture of trace elements> Added insulin for growth enhancement> Added iron-binding molecules for improved uptake of

iron ions> Supplemented with selected serum components with

growth factors

Advantages

> Ready to use> Optimized for fibroblasts> Defined culture conditions> No addition of serum necessary> Reproducible working conditions> Minimizes the risk of contamination

Composition

Modified formulation based on MEM with a new bal-anced mixture of trace elements.


CO2 -Concentration, optimum . . . . . . . . . . . 5.0 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 25 EU/ml

Cell Culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . testedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . . 3 months at +2°C to +8°C . . . . . . . . . . . . . . . . . . . . . . . . . 2 years at ≤ −15°C

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Formulation mg/l

Inorganic SaltsCalcium Chloride anhydrous . . . . . . . . . . . . . . . . . . . . . 200.00Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400.00Magnesium Sulphate anhydrous . . . . . . . . . . . . . . . . . . . 97.70Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6800.00Sodium Dihydrogen Phosphate • H2O . . . . . . . . . . . . . . 140.00Sodium Hydrogen Carbonate. . . . . . . . . . . . . . . . . . . . 2200.00

Amino AcidsL-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26.00L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 292.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . . . 42.00L-Isoleucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.50L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40.00

VitaminsD-Calcium Pantothenate . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.50Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.10Thiamine • HCl. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . 3400.00Phenol Red. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*Iron binding molecules . . . . . . . . . . . . . . . . . . . . . . prop. info.*Protein Fractions . . . . . . . . . . . . . . . . . . . . . . . . . . prop. info.*



Sera

Sera

Foetal Bovine Serum (FBS) 50

FBS Gold . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50FBS Standard Quality . . . . . . . . . . . . . . . . . . . 51FBS Clone . . . . . . . . . . . . . . . . . . . . . . . . . . . 51FBS Gamma Irradiated . . . . . . . . . . . . . . . . . . 52FBS Heat Inactivated . . . . . . . . . . . . . . . . . . . 53FBS IgG Stripped . . . . . . . . . . . . . . . . . . . . . . 54FBS Dialysed . . . . . . . . . . . . . . . . . . . . . . . . . 54FBS ES cell tested . . . . . . . . . . . . . . . . . . . . . . 55FBS Amnion cell tested . . . . . . . . . . . . . . . . . 55FBS Tetracycline negative . . . . . . . . . . . . . . . . 56FBS Mycoplex . . . . . . . . . . . . . . . . . . . . . . . . 56FBS Pharma Grade . . . . . . . . . . . . . . . . . . . . . 57

Bovine Sera 58

Neo-natal FBS . . . . . . . . . . . . . . . . . . . . . . . . 58Newborn Calf Serum . . . . . . . . . . . . . . . . . . . 59Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . 60Donor Calf Serum . . . . . . . . . . . . . . . . . . . . . 60Bovine Serum . . . . . . . . . . . . . . . . . . . . . . . . 61Donor Bovine Serum . . . . . . . . . . . . . . . . . . . 61

Horse Sera 62

Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . . 62Donor Horse Serum . . . . . . . . . . . . . . . . . . . . 62

Other Animal Sera 63

Porcine Serum . . . . . . . . . . . . . . . . . . . . . . . . 63Sheep Serum . . . . . . . . . . . . . . . . . . . . . . . . . 63Rabbit Serum . . . . . . . . . . . . . . . . . . . . . . . . 63Goat Serum . . . . . . . . . . . . . . . . . . . . . . . . . 63Rat Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . 63Mouse Serum . . . . . . . . . . . . . . . . . . . . . . . . 63

Human Sera 64

Human Serum, off the clot . . . . . . . . . . . . . . . 64Human Serum, converted . . . . . . . . . . . . . . . 65


Sera

SeraAnimal and human sera are used as the main source for growth factors, hormones and other cell stimulating components. Sera from PAA arecharacterized by high quality, sterility andhomogeneity.

49

Sera

PAA`s production facilities are fully compliant with cur-rent Good Manufacturing Practices (cGMP) operatingunder a pharmaceutical grade licence. PAA controls allprocesses from raw material collection through to finalsterile filtered product guaranteeing full product verticalintegration. The supply chain follows our strict qualitycontrol standards and regular audits are performed toensure all procedures are within our quality assurancestandards. Constant monitoring of all processes ensuresthat only products of the highest quality with batch tobatch consistency are manufactured by PAA.

Origin

PAA collects raw serum from both United StatesDepartment of Agriculture (USDA) and European (EU)approved sources. Both high quality and biologicalsafety are key factors along with traceability and docu-mentation control. PAA can offer its customers fullydocumented serum products from Australia, USA,Canada and Central America which are all USDAapproved origins. For customers not requiring USDAsourced sera then EU approved versions are available.PAA holds several Certificates of Suitability (CoS).

Manufacturing

To fulfil the increasing demand for sera, particularly fromthe industrial market, PAA has opened production sitesin Australia (Brisbane) and Canada (Toronto) to comple-ment its ‘state of the art’ production facility in Austria(Linz). Therefore, raw material collection through to thefinal sterile filtered product is performed locally. All PAAproduction sites operate under cGMP guidelines.

Production

To standardize production processes, all PAA productionsites are controlled by the same documented and con-trolled SOP's. Raw material is filtered under temperaturecontrolled (cooled) conditions through a filter cascadeprocess down to triple 100nm size filters. The maximumbatch size is presently 2.600 litres. Throughout the fullproduction process in-line monitoring and testing ensu-res that only high quality serum products are releasedfor sale to our customers. Batch process and productionrecords are prepared along with all other necessarydocuments including a comprehensive Certificate ofAnalysis (CoA).

Test samples, Reservations and Orders

Many customers prefer to test serum products withtheir respective cell lines in line with their in-houserequirements. PAA will provide samples from differentbatches for customer testing and will hold the reques-ted numbers of bottles from each batch on reservationuntil sample tests are completed. Normally reservationsare held for 4 weeks. Please advise PAA if longer reser-vation periods are required.

For those customers placing orders, who do not havesufficient storage space, PAA can store their serum incontrolled freezer rooms for future ‘call off’ or ‘soldand storage’ orders. For full details on these pleasecontact PAA.

Storage, Shipping and Logistics

All sera are stored in ≤ -15°C controlled freezer roomsat PAA facilities.

Serum should be shipped frozen at this temperature.PAA ships all serum products in styrofoam boxes con-taining sufficient solid CO2 (dry ice) to maintain frozenconditions during shipment. Upon receipt please placeall sera products in a ≤ -15°C freezer.

PAA – The Cell Culture Company | www.paa.com

Introduction

Foetal Bovine Serum "GOLD"Defined Foetal Bovine Serum

Cat. No. Volume

Foetal Bovine Serum "GOLD"Defined Foetal Bovine SerumAUS origin A11-551 100 ml

A15-551 500 ml

Foetal Bovine Serum "GOLD"Defined Foetal Bovine SerumUSA origin A11-251 100 ml

A15-251 500 ml

Foetal Bovine Serum "GOLD"Defined Foetal Bovine SerumCAN origin A11-751 100 ml

A15-751 500 ml

Foetal Bovine Serum "GOLD"Defined Foetal Bovine SerumUSDA approved A11-351 100 ml

A15-351 500 ml

Foetal Bovine Serum "GOLD"Defined Foetal Bovine SerumEU approved* A11-151 100 ml

A15-151 500 ml

Applications

> Suitable for MRC5-, HeLa-, 3T3-, Sf9-, CHO-,Hybridoma-, primary and neuronal cells

> Calibration assays> For fermentation> Stabilizer for diagnostic kits

Features

> Fully defined FBS> Low IgG content> Low haemoglobin content> FBS GOLD is produced using a chemical separation

process. After separation the various componentsare reconstituted

> Not a synthetic product> Tested on cell culture> Batch sizes up to 2600 litres available

Advantages

> Once tested – always same quality> No batch to batch variations> No extra costs > No batch testing necessary> No batch reservation necessary


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 30 EU/mlTotal Protein . . . . . . . . . . . . . . . . . . 3.0 – 4.5 g/dlAlbumin . . . . . . . . . . . . . . . . . . . . . 1.7 – 3.4 g/dlIgG . . . . . . . . . . . . . . . . . . . . . . . . . . < 100 µg/mlHaemoglobin . . . . . . . . . . . . . . . . . . . < 20 mg/dl

Mycoplasma . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . PI-3, BVD-AB, BVD-V, BHV-ISterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . 5 yearsStorage . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

For each batch a Certificate of Analysis can be reques-ted with detailed results of our quality control.

* Not available in North America due to USDA restrictions.


Sera


Sera

Foetal Bovine SerumStandard Quality

Foetal Bovine SerumPAA Clone

Cat. No. Volume

Foetal Bovine Serum Standard QualityAUS origin A11-501 100 ml

A15-501 500 ml

Foetal Bovine Serum Standard QualityUSA origin A11-201 100 ml

A15-201 500 ml

Foetal Bovine Serum Standard QualityCAN origin A11-701 100 ml

A15-701 500 ml

Foetal Bovine Serum Standard QualityUSDA approved A11-301 100 ml

A15-301 500 ml

Foetal Bovine Serum Standard QualityEU approved* A11-101 100 ml

A15-101 500 ml

Applications

> Cell culture additive> Supports most generally used cell types> Stabilizer for diagnostic kits> Surface coating> Inactivation of trypsin

Features

> Complete documentation available> Tested for viruses and microorganisms> Tested on cell culture> Batch sizes up to 2600 litres available> Clear appearance


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . valueTotal Protein . . . . . . . . . . . . . . . . . . 3.0 – 4.5 g/dlAlbumin . . . . . . . . . . . . . . . . . . . . . 1.7 – 3.4 g/dlIgG . . . . . . . . . . . . . . . . . . . . . . . . . . < 250 µg/mlHaemoglobin . . . . . . . . . . . . . . . . . . . < 20 mg/dl

Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . PI-3, BVD-AB, BVD-V, BHV-ISterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested


For each batch a Certificate of Analysis can be reques-ted for detailed results of our quality control.


Cat. No. Volume

Foetal Bovine Serum PAA CloneUSA origin A11-202 100 ml

A15-202 500 ml

Foetal Bovine Serum PAA CloneEU approved* A11-102 100 ml

A15-102 500 ml

Applications

> Suitable for all cell types> Hybridoma cloning> Sensitive cells> Primary cell culture

Features

> Endotoxin lower than 1 EU/ml> Highest viability > Highest growth rates> Increased plating efficiency


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/mlTotal Protein . . . . . . . . . . . . . . . . . . 3.0 – 4.5 g/dlAlbumin . . . . . . . . . . . . . . . . . . . . . 1.7 – 3.4 g/dlIgG . . . . . . . . . . . . . . . . . . . . . . . . . . < 250 µg/mlHaemoglobin . . . . . . . . . . . . . . . . . . . < 20 mg/dl





Foetal Bovine Serum Gamma Irradiated

Cat. No. Volume

Foetal Bovine Serum Gamma IrradiatedAUS origin A11-503 100 ml

A15-503 500 ml

Foetal Bovine Serum Gamma IrradiatedUSA origin A11-203 100 ml

A15-203 500 ml

Foetal Bovine Serum Gamma IrradiatedCAN origin A11-703 100 ml

A15-703 500 ml

Foetal Bovine Serum Gamma IrradiatedUSDA approved A11-303 100 ml

A15-303 500 ml

Foetal Bovine Serum Gamma IrradiatedEU approved* A11-103 100 ml

A15-103 500 ml

Applications

> Biopharmaceutical productions> Virus production> Vaccine production> Manufacture of diagnostic products

Features

> Complete documentation available> Sterile filtered> Tested for viruses and microorganisms

Gamma Irradiation

> Serum is irradiated with at least 25 kGy> Reduction of gamma-sensitive viruses and mycoplasma> Irradiation can be performed up to 35 kGy upon

special request> Validated protocols available








Sera

Foetal Bovine Serum Heat Inactivated


Sera

Cat. No. Volume

Foetal Bovine SerumHeat InactivatedAUS origin A11-504 100 ml

A15-504 500 ml

Foetal Bovine SerumHeat InactivatedUSA origin A11-204 100 ml

A15-204 500 ml

Foetal Bovine SerumHeat InactivatedCAN origin A11-704 100 ml

A15-704 500 ml

Foetal Bovine SerumHeat InactivatedUSDA approved A11-304 100 ml

A15-304 500 ml

Foetal Bovine SerumHeat InactivatedEU approved* A11-104 100 ml

A15-104 500 ml

Applications

> Growth factor for cell culture> Supports most cell types> Stabilizer for diagnostic kits> Surface coating> Inactivation of trypsin

Features

> Sterile filtered> Tested for viruses and microorganisms> Complement factors are destroyed> Different enzyme activities are reduced> Cell culture tested

Heat Inactivation

> Heating the serum to +56°C and maintaining thistemperature for 30 minutes

> Produced in a controlled environment> Ability to reproduce a consistently accurate heat

inactivation process> Inactivation of complement factors> Reduction of enzyme activities








Sera

Foetal Bovine SerumIgG Stripped

Foetal Bovine Serum Dialysed

Cat. No. Volume

Foetal Bovine Serum IgG StrippedUSA origin A11-206 100 ml

A15-206 500 ml

Foetal Bovine Serum IgG StrippedEU approved* A11-106 100 ml

A15-106 500 ml

Applications

> Hybridoma technology> Vaccine production> Manufacture of diagnostic kits> Biopharmaceutical production> Virus production

Features

> IgG content lower than 5 µg/ml> Sterile filtered> Tested for viruses and microorganisms


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . valueTotal Protein . . . . . . . . . . . . . . . . . . 3.0 – 4.5 g/dlAlbumin . . . . . . . . . . . . . . . . . . . . . .1.7 – 3.4 g/dl IgG . . . . . . . . . . . . . . . . . . . . . . . . . . . . < 5 µg/mlHaemoglobin . . . . . . . . . . . . . . . . . . . < 20 mg/dl





Cat. No. Volume

Foetal Bovine Serum DialysedAUS origin A11-507 100 ml

A15-507 500 ml

Foetal Bovine Serum DialysedUSA origin A11-207 100 ml

A15-207 500 ml

Foetal Bovine Serum DialysedCAN origin A11-707 100 ml

A15-707 500 ml

Foetal Bovine Serum DialysedUSDA approved A11-307 100 ml

A15-307 500 ml

Foetal Bovine Serum DialysedEU approved* A11-107 100 ml

A15-107 500 ml

Applications

> Radioactive incorporation studies> Minimal essential growth supplement> Hormone free cell culture

Features

> Sterile filtered> Tested for viruses and microorganisms> Cell culture tested

Dialysis

> Dialysis is performed with a cut off of 10 kDa> Dialysis against a 0.15 M physiological saline solution> Dialysis until a glucose content lower than 10.0 mg/dl


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . valueTotal Protein . . . . . . . . . . . . . . . . . . 3.0 – 4.5 g/dlAlbumin . . . . . . . . . . . . . . . . . . . . . . 1.7 - 3.4 g/dlIgG . . . . . . . . . . . . . . . . . . . . . . . . . . < 250 µg/mlHaemoglobin . . . . . . . . . . . . . . . . . . . < 20 mg/dl

Mycoplasma . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . PI-3, BVD-AB, BVD-V, BHV/ISterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested





Sera

Foetal Bovine SerumES cell tested

Foetal Bovine SerumAmnion cell tested

Cat. No. Volume

Foetal Bovine SerumES cell tested A11-208 100 ml

A15-208 500 ml

General

Foetal Bovine Serum, pre-tested on embryonic stemcells is specially tested for the ability to sustain undif-ferentiated cellular morphology of embryonic stemcells. For testing the mouse ES cell line D3 is cultivatedin the presence of leukaemia inhibitory factor (LIF). Thescreening includes plating efficiency, colony morpholo-gy and toxicity tests.

Applications

> Supports the growth of undifferentiated colonies ofES cells

> Recommended for most primary cells > For low unspecific stimulation

Features

> Special serum batches preselected by the colonymorphology and the expression of different stem cellmarkers

> Low endotoxin content> Tested for viruses and microorganisms


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/mlTotal Protein . . . . . . . . . . . . . . . . . . 3.0 – 4.5 g/dlAlbumin . . . . . . . . . . . . . . . . . . . . . 1.7 – 3.4 g/dlIgG . . . . . . . . . . . . . . . . . . . . . . . . . . < 250 µg/mlHaemoglobin . . . . . . . . . . . . . . . . . . . < 20 mg/dl




Cat. No. Volume

Foetal Bovine SerumAmnion cell tested A11-110 100 ml

A15-110 500 ml

General

Foetal Bovine Serum, pre-tested on amnion cells isspecially tested for the attachment of cells, for thegrowth capability of cells, the ratio between fibrob-lasts and epithelial cells and for chromosomal mor-phology.

Applications

> Cultivation of amnion and chorionic villi cells> Cultivation of lymphocytes> Pre- and postnatal diagnostics> Karyotyping> Fluorescence In Situ Hybridization (FISH analysis)

Features

> Special serum batches preselected by quantitativegrowth assays using amnion and chorionic villi cells

> Tested for viruses and microorganisms







Sera

Foetal Bovine SerumTetracycline negative

Foetal Bovine SerumMycoplex

Cat. No. Volume

Foetal Bovine SerumTetracycline negativeUSA origin A11-209 100 ml

A15-209 500 ml

Foetal Bovine SerumTetracycline negativeEU approved* A11-109 100 ml

A15-109 500 ml

Applications

> TET-On / TET-Off gene expression system> Transfection> Expression studies

Features

> Tetracycline negative> Tetracycline derivates negative


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/mlTotal Protein . . . . . . . . . . . . . . . . . . 3.0 – 4.5 g/dlAlbumin . . . . . . . . . . . . . . . . . . . . . 1.7 – 3.4 g/dlIgG . . . . . . . . . . . . . . . . . . . . . . . . . . < 250 µg/mlHaemoglobin . . . . . . . . . . . . . . . . . . . < 20 mg/dlTetracyclin /-derivates . . . . . . . . . . . . ≤ 0.5 µg/ml





Cat. No. Volume

Foetal Bovine Serum MycoplexAUS origin A11-505 100 ml

A15-505 500 ml

Foetal Bovine Serum MycoplexUSA origin A11-205 100 ml

A15-205 500 ml

Foetal Bovine Serum MycoplexEU approved* A11-105 100 ml

A15-105 500 ml

Applications

> Cell culture additive> Supports most cell types> Mycoplasma free cell culture> Primary cell culture> Manufacture of diagnostics and therapeutics by bio-

technological fermentation

Features

> Sterile filtered> UV-treated> Guaranteed free of mycoplasma

UV Inactivation

Mycoplex is FBS treated by a validated UV inactivationprocess to destroy viruses and mycoplasma in a sensi-tive manner. Any potential contamination in the serumis definitely eliminated.



Mycoplasma . . . . . . . . . . . . . . . . . . . not detected Virus tested for . . . . . PI-3, BVD-V, BVD-AB, BHV-ISterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested





Sera

Foetal Bovine SerumPharma Grade

Cat. No. Volume

FBS Pharma GradeAUS origin A11-511 100 ml

A15-511 500 ml

FBS Pharma GradeGamma IrradiatedAUS origin A11-512 100 ml

A15-512 500 ml

FBS Pharma GradeUSA origin A11-211 100 ml

A15-211 500 ml

FBS Pharma GradeGamma IrradiatedUSA origin A11-212 100 ml

A15-212 500 ml

General

FBS Pharma Grade is a high quality Foetal BovineSerum which complies with all current directives,regulations and guidelines regulating the manufactureof biopharmaceutical products. These regulationscomprise the EMEA guideline 1793/02 of thecommittee for proprietary medical products (CPMP),EMEA guideline 743/00 of the committee for vete-rinary medical products (CVMP), the Ph. Eur. (EuropeanPharmacopoeia) 5.4 monograph of Bovine Serum(04/2006:2262) and FDA's Code of Federal Regulation(9CFR). An extensive test panel of mycoplasma, tendifferent viruses, BVD antibodies and BVD antibodyinterferences as well as biochemical and cell cultureparameters minimize the risks in biopharmaceuticalprocesses. FBS Pharma Grade is suitable for cell based productswhich need to be produced and registered in comp-liance with the EU regulation 2005/567 for AdvancedTherapies (AT).

Applications

> Biopharmaceutical industry> Vaccine production> Production of recombinant antibodies> Production of recombinant growth factors> Production of advanced therapy products

Features

> Tested according to Ph. Eur. 5.4 monograph04/2006:2262

> Tested according to CPMP/BWP 1793/02> Tested according to CVMP/ 743/00> Tested according to 9CFR> No BVD antibody interferences> 35kGy effective single box gamma irradiation> Batch size up to 2.600 litres> Australian and U.S. origin*

Gamma Irradiation

> Reduction of gamma sensitive viruses and mycoplasma

> Single box irradiation ensures low maximum energy dosage

> Irradiation dosage of 35 kGy > Ensured constant low temperature> Less damage of sensitive serum components> Batch homogeneity not influenced


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . . 280 – 340 mOsmol/kgTotal Protein . . . . . . . . . . . . . . . . . . . 3.0 – 4.5 g/dlAlbumin . . . . . . . . . . . . . . . . . . . . . . 1.7 – 3.4 g/dlEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 25 EU/mlIgG . . . . . . . . . . . . . . . . . . . . . . . . . . . < 250 µg/mlHaemoglobin . . . . . . . . . . . . . . . . . . . . < 20 mg/dl

Sterility . . . . . . . . . . . . . . . . . . . . . . . . . . . . testedMycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . . Parainfluenza Type 3 (PI-3)

Bovine Virus Diarrhoea Virus (BVD-V)Bovine Herpes Virus Type 1 (BHV-1)

Blue Tongue Virus (BTV), Bovine Adenovirus (BAV)Bovine Reovirus (BRV), Bovine Parvovirus (BPV)

Bovine Respiratory Syncytial Virus (BRSV)Rabies Virus, BVD-1 AB, BVD-2 AB, Rabies AB

BVD AB Inhibitory, BVD AB Interferences

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . . 5 yearsStorage . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ -15 °C

For each batch complete documentation can berequested.

* Other origins available on request


Sera

Cat. No. Volume

Neo-natal FBSUSDA approved A11-315 100 ml

A15-315 500 ml

Neo-natal FBSHeat InactivatedUSDA approved A11-316 100 ml

A15-316 500 ml

Neo-natal FBSGamma IrradiatedUSDA approved A11-317 100 ml

A15-317 500 ml

General

Neo-natal Foetal Bovine Serum is collected from calvesless than 12 hours old, prior to ingestion of colostrums.Neo-natal FBS is an economical alternative to FoetalBovine Serum with similar growth promotion characte-ristics to FBS.

Applications

> Cell culture additive> Manifold cell culture applications> Fermentation> Surface coating

Features

> Contains more growth factors than Newborn CalfSerum

> Cost-effective alternative to FBS> Increased protein content

Heat Inactivation




Gamma Irradiation


special request


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.7Osmolality . . . . . . . . . . . . . 260 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . valueTotal Protein . . . . . . . . . . . . . . . . . . 5.0 – 7.5 g/dlAlbumin . . . . . . . . . . . . . . . . . . . . . 2.5 – 5.0 g/dlHaemoglobin . . . . . . . . . . . . . . . . . . . < 20 mg/dl

Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . PI-3, BVD-V, BVD-AB, BHV-ISterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested



Neo-natal Foetal Bovine Serum


Sera

Newborn Calf Serum

Cat. No. Volume

Newborn Calf SerumB11-001 100 mlB15-001 500 ml

Newborn Calf SerumHeat Inactivated B11-102 100 ml

B15-102 500 ml

Newborn Calf SerumGamma Irradiated B11-303 100 ml

B15-303 500 ml

Applications

> Cell culture additive> Manifold cell culture applications> Fermentation> Surface coating

Features

> Contains more immunoglobulins than Foetal BovineSerum

> Increased protein content> Cost-effective alternative to FBS

Heat Inactivation




Gamma Irradiation


special request



Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . . . PI-3, BVD-V, BVD-AB, BHV-ISterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested




Sera

Calf Serum Donor Calf Serum

Cat. No. Volume

Calf SerumB11-004 100 mlB15-004 500 ml

Calf SerumHeat Inactivated B11-106 100 ml

B15-106 500 ml

Calf SerumGamma Irradiated B11-307 100 ml

B15-307 500 ml

Applications

> Short term culture of cells with low sensitivity> Fermentation> Stabilizer for enzyme assays> Serum control standard> Surface coating

Features

> High amount of IgG antibodies> Cost-effective alternative to FBS

Heat Inactivation


> Inactivation of complement factors> Reduction of enzyme activities

Gamma Irradiation


special request






Cat. No. Volume

Donor Calf SerumB11-008 100 mlB15-008 500 ml

Donor Calf SerumHeat Inactivated B11-109 100 ml

B15-109 500 ml

Donor Calf SerumGamma Irradiated B11-310 100 ml

B15-310 500 ml

Applications

> Cell culture additive> Stabilizer for diagnostic kits> Control standard> Source for bovine proteins

Features

> Sterile filtered> Controlled collection

Heat Inactivation



Gamma Irradiation


special request







Sera

Bovine Serum Donor Bovine Serum

Cat. No. Volume

Bovine SerumB11-011 100 mlB15-011 500 ml

Bovine SerumHeat Inactivated B11-112 100 ml

B15-112 500 ml

Bovine SerumGamma Irradiated B11-313 100 ml

B15-313 500 ml

Applications

> Cell culture additive> Control standard> Dilution matrix> Calibration

Features

> High batch conformity> Low haemoglobin content> Sterile filtered

Heat Inactivation



Gamma Irradiation


special request






Cat. No. Volume

Donor Bovine SerumB11-063 100 mlB15-063 500 ml

Donor Bovine SerumHeat Inactivated B11-164 100 ml

B15-164 500 ml

Donor Bovine SerumGamma Irradiated B11-365 100 ml

B15-365 500 ml

Applications

> Cell culture additive> Control standard> Reduction of unspecific activities within ELISA test

systems> Calibration

Features

> Lower endotoxin level than bovine serum> No toxic by products released during slaughtering

Heat Inactivation



Gamma Irradiation


special request







Sera

Horse Serum Donor Horse Serum

Cat. No. Volume

Horse SerumB11-021 100 mlB15-021 500 ml

Horse SerumHeat Inactivated B11-122 100 ml

B15-122 500 ml

Features

> Alternative to Calf Serum> Low haemoglobin content> Low content of fatty acids> Collected in approved abattoirs

Heat Inactivation

> To reduce complement factors> Heating the serum to +56°C and maintaining this

temperature for 30 minutes> Inactivation of enzyme activities



Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . EIA (Equine Infectious Anaemia)Sterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested



Cat. No. Volume

Donor Horse SerumB11-023 100 mlB15-023 500 ml

Donor Horse SerumHeat Inactivated B11-124 100 ml

B15-124 500 ml

Features

> Raw serum contains a low content of contaminants> Low endotoxin content> An alternative to Calf Serum

Heat Inactivation

> To reduce complement factors> Heating the serum to +56°C and maintaining this

temperature for 30 minutes> Inactivation of enzyme activities



Mycoplasma . . . . . . . . . . . . . . . . . . . not detected Virus tested for . . EIA (Equine Infectious Anaemia)Sterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested




Sera

Other Animal Sera

Cat. No. Volume

Porcine Serum B11-030 100 mlB15-030 500 ml

Sheep Serum B11-032 100 mlB15-032 500 ml

Rabbit Serum B11-034 100 ml

Goat Serum B11-035 100 mlB15-035 500 ml

Rat Serum B02-037 20 mlB11-037 100 ml

Mouse Serum B02-038 20 mlB11-038 100 ml

Applications

> Autologous cell culture systems> Immune histochemistry> Immunoblotting> Immunofluorescence> Immunoprecipitation

Test Parameters

> pH> Osmolality> Endotoxin> Total Protein> Haemoglobin> Mycoplasma> Sterility

Storage




Sera

Human Serumoff the clot

Cat. No. Volume

Human Serumoff the clot C11-020 100 ml

C15-020 500 ml

Human Serumoff the clotType AB C11-021 100 ml

C15-021 500 ml

Applications

> Additive for sensitive cell cultures> Suitable for most human cells> Recommended for lymphocytes and human macro-

phages

Features

> Collection in FDA inspected plasmapheresis centres> No anticoagulant added> Naturally clotted> Without fibrin> Human Serum, off the clot Type AB from donors

without AB antibodies

Manufacture

> From whole blood without anticoagulant> Single-use blood donor bags > Natural blood coagulation > Centrifugation> Sterile filtered



Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . HBAg, HCV, Anti HIV1/2, TPHASterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested



Precaution

All Human Sera have been tested internally and exter-nally according to our strict guidelines. However allhuman products should be treated as potentially infec-tious agents and in accordance with universal handlingprecautions. All Human Sera of PAA should only beused for in vitro diagnostic and research purposes, notfor therapeutic use.


Sera

Human Serumconverted

Cat. No. Volume

Human Serum converted C11-001 100 ml

C15-001 500 ml

Human Serum convertedType AB C11-002 100 ml

C15-002 500 ml

Applications

> Base for assay controls> Additive for cell culture> Stabilizer in immunoassays> Clinical chemistry > In vitro diagnostic use

Features

> Collection in FDA inspected plasmapheresis centres> Human Serum, converted Type AB from donors

without AB antibodies

Manufacture

> Converted from plasma with citrate-phosphate-dex-trose

> Fibrinogen is transformed to fibrin> Sterile filtered



Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus tested for . . . HBAg, HCV, Anti HIV1/2, TPHASterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested



Precaution

All Human Sera have been tested internally and exter-nally according to our strict guidelines. However allhuman products should be treated as potentially infec-tious agents and in accordance with universal handlingprecautions. All Human Sera of PAA should only beused for in vitro diagnostic and research purposes, notfor therapeutic use.


Reagents

Reag

ents

Amino Acids & Vitamins 70

MEM Amino Acid Concentrate . . . . . . . . . . . . 70NEAA Amino Acid Concentrate . . . . . . . . . . . 70L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . 71Stable Glutamine . . . . . . . . . . . . . . . . . . . . . . 71L-Glutamine with Pen/Strep . . . . . . . . . . . . . . 72MEM Vitamins . . . . . . . . . . . . . . . . . . . . . . . . 72

Antibiotics 73

Amphotericin B . . . . . . . . . . . . . . . . . . . . . . . 73Antibiotic-Antimycotic Solution . . . . . . . . . . . 73Gentamicin . . . . . . . . . . . . . . . . . . . . . . . . . . 74Penicillin/Streptomycin . . . . . . . . . . . . . . . . . . 74MycoKill AB . . . . . . . . . . . . . . . . . . . . . . . . . 75

Detachment 76

Accutase . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76Alfazyme . . . . . . . . . . . . . . . . . . . . . . . . . . . 76AccuMax . . . . . . . . . . . . . . . . . . . . . . . . . . . 77Collagenase 2 . . . . . . . . . . . . . . . . . . . . . . . . 77Trypsin . . . . . . . . . . . . . . . . . . . . . . . . . . 78 - 81

Growth Supplements 82

Hybridoma Cloning Factor . . . . . . . . . . . . . . . 82HematoStem . . . . . . . . . . . . . . . . . . . . . . . . . 82B27 NeuroMix . . . . . . . . . . . . . . . . . . . . . . . . 83G5 Supplement . . . . . . . . . . . . . . . . . . . . . . . 83N2 Supplement . . . . . . . . . . . . . . . . . . . . . . . 84Neuronal Stem Cell Supplement . . . . . . . . . . . 84

Transfection 85

Nanofectin Kit . . . . . . . . . . . . . . . . . . . . . . . . 85G-418 Sulphate . . . . . . . . . . . . . . . . . . . . . . . 86Puromycin . . . . . . . . . . . . . . . . . . . . . . . . . . . 87Blasticidin . . . . . . . . . . . . . . . . . . . . . . . . . . . 87Hygromycin B . . . . . . . . . . . . . . . . . . . . . . . . 88Carbenicillin . . . . . . . . . . . . . . . . . . . . . . . . . 88

Other Reagents 89

Lymphocyte Separation Medium . . . . . . . . . . . 89Bovine Serum Albumin . . . . . . . . . . . . . . 90 - 92Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . 92Giemsa Stain Solution . . . . . . . . . . . . . . . . . . 93Potassium Chloride . . . . . . . . . . . . . . . . . . . . 93Colcemid . . . . . . . . . . . . . . . . . . . . . . . . . . . 94Phytohaemagglutinin (PHA) . . . . . . . . . . . . . . 94CryoMaxx . . . . . . . . . . . . . . . . . . . . . . . . . . . 95CoolStar . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96


Reag

ents

ReagentsReagents in cell culture are used for many differentpurposes. To meet the customers demands allreagents of PAA are intensively tested for purity,toxicity and cell functionality.


Reag

ents

PAA manufactures and supplies a wide range of highquality reagents for use in cell culture. These reagentsare supplied as a separate product group for use inconjunction with PAA serum products, cell culturemedia and buffer solutions.

Raw Materials

All chemicals and other raw materials are subject tostringent quality tests before being released out ofquarantine into our cell culture production areas. Onlythose materials which meet with PAA documentedQuality Control & Quality Assurance (QC/QA) internalstandards are allowed for use in our production facilities.

Production

The liquid reagents are all sterile filtered and then filledunder Laminar Air Flow (LAF) conditions. They are pro-vided as either liquids or frozen solutions dependingupon individual product storage criteria.

Water purity is a very important factor in the produc-tion of high quality reagents. All reagents are preparedusing purified water which meets stringent industrystandards.

All reagents sold in powder format are also subject tostrict quality standards.

Quality Control

PAA reagents are subject to strict QC testing beforerelease. These tests include endotoxin level (LPS), pHand osmolality. Certain reagents are also tested forbiological performance. Other QC tests include celldetachment/attachment, transfection assays and anti-biotic toxicity activity. Growth tests for specific celltypes such as embryonic and neuronal stem cells andamniocytes are also performed.

Packaging

All liquid reagents are sterile filtered into gamma irra-diated PET bottles. All reagents supplied in powderform are filled into convenient sized containers.

Storage & Shipping

Exact storage conditions are shown on each productlabel and in the product specification as shown in thiscatalogue. Some products must be stored at ≤ −15°C,others at +2°C to +8°C and some at room temperatu-re. Please ensure you take careful note of the storageconditions to ensure product integrity.

PAA provides products in appropriate packaging tomeet storage requirements.

All products are subject to a final quality control checkbefore being packaged and despatched.

Introduction

MEM Amino Acids

MEM Non Essential Amino Acids (NEAA)

Cat. No. Volume

MEM Amino Acids 50x Concentrate M11-002 100 ml

Applications

> Amino acid solution for Basal Medium Eagle´s MEM> Additional source of amino acids


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.0 – 2.0Osmolality . . . . . . . . . . . . . 400 – 600 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/ml



Formulation mg/l

L-Arginine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6320.00L-Cystine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1201.00L-Histidine • HCl • H2O . . . . . . . . . . . . . . . . . . . . . . . . 2096.00L-Isoleucine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2623.00L-Leucine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2623.00L-Lysine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3625.00L-Methionine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 755.00L-Phenylalanine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1651.00L-Threonine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2382.00L-Tryptophan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 510.00L-Tyrosine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1811.00L-Valine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2343.00

Cat. No. Volume

MEM NEAA100x Concentrate M11-003 100 ml

Applications

> Cells, which have lost the ability to synthesize nonessential amino acids, have to be supplemented withthese amino acids

> Additional amino acid pool for strong proliferating cells


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.0 – 4.0Osmolality . . . . . . . . . . . . . . 50 – 100 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/ml


Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . 2 yearsStorage . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Formulation mg/l

L-Alanine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 890.00L-Asparagine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1320.00L-Aspartic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1330.00L-Glutamic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1470.00L-Glycine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 750.00L-Proline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1150.00L-Serine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1050.00


Reag

ents


Reag

ents

L-Glutamine Stable Glutamine

Cat. No. Volume

L-Glutamine 200 mM Concentrate M11-004 100 ml

Applications

> Essential component of cell culture media> L-Glutamine is sensitive to temperature and should

be added to a medium immediately before use

Note: Toxic degeneration products of L-glutamine(ammonium ions) can interfere and may cause dama-ge to cell walls.





Formulation

L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200 mM

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.9 % NaCl

Cat. No. Volume

Stable Glutamine 200 mM Concentrate M11-006 100 ml

General

L-Glutamine is one of the crucial components in cellculture media. L-Glutamine in liquid media degradeswithin a few days when cultured in +37°C.Stable Glutamine is a stable dipeptide form of L-gluta-mine. L-alanyl-L-glutamine does not degrade in liquidmedia incubated at +37°C or stored at +15°C to+25°C.

Applications

> Direct substitute for L-glutamine> For mammalian cell cultures> For suspension and adherent cell cultures> Essential media component

Features

> Stabilized form of L-glutamine> No toxic ammonia build-up due to degradation

processes





Formulation

L-Alanyl-L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . 200 mM

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.9 % NaCl

L-Glutamine (200 mM) withPenicillin and Streptomycin MEM Vitamins

Cat. No. Volume

L-Glutamine with Penicillin/Streptomycin100x Concentrate P11-013 100 ml

Applications

> L-Glutamine is an essential amino acid, a carbon andenergy source for cells

> Penicillin inactivates the bacterial cell wall synthesisof gram negative bacteria

> Streptomycin blocks the protein biosynthesis ofgram positive bacteria

Features

> Minimizes cross contamination> Has the ability to reproduce results to constant

standard

Working Concentration

Recommended final concentration: . . . . . . . 10 ml/l




Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . 2 yearsStorage . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°CStability . . . . . . . . . . . . . . . . . . . 5 days at +37°C

Formulation

L-Glutamine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29.20 g/lPenicillin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.000 Units/mlStreptomycin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 mg/ml

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.9 % NaCl

Cat. No. Volume

MEM Vitamins100x Concentrate N11-002 100 ml





Formulation mg/l

Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8500.00Cholin Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100.00Folic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100.00Myo-Inositol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200.00Nicotinamide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100.00D-Pantothenic Acid (hemicalcium) . . . . . . . . . . . . . . . . . 100.00Pyridoxal • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100.00Riboflavin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.00Thiamine • HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100.00

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.9% NaCl


Reag

ents


Reag

ents

Amphotericin B Antibiotic–Antimycotic Solution

Cat. No. Volume

Amphotericin B P11-001 100 ml

General

Amphotericin B is an antifungal agent that is used intissue culture for the control of yeasts and fungi. It actsby binding to steroidal alcohols in the cell membraneof susceptible fungi. Amphotericin B creates channelsin the cell membrane resulting in an increase of mem-brane permeability. The loss of cations through thechannels into the extracellular space and the inhibitionof a number of membrane bound enzymes destroy theyeast or fungus. Amphotericin B has no effect on bac-teria and viruses.

Applications

> Antifungal spectrum> Interacts with membranes of fungi> Forms channels in membranes




pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.5 – 8.0Osmolality . . . . . . . . . . . . . . . . 0 – 50 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/ml



Formulation

Amphotericin B . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.25 mg/ml

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Water

Cat. No. Volume

Antibiotic-AntimycoticSolution P11-002 100 ml

Applications

> Broad range antibiotic> Antifungal spectrum (Amphotericin B)> Suitable for contaminated cells> Is recommended as primary cell line prophylaxis







Formulation

Penicillin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.000 Units/mlStreptomycin Sulphate. . . . . . . . . . . . . . . . . . . . . . . . 10 mg/mlAmphotericin B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 µg/ml

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.9 % NaCl

Gentamicin Penicillin / Streptomycin (100x)

Cat. No. Volume

Gentamicin(10 mg/ml) P11-004 100 ml

Gentamicin(50 mg/ml) P11-005 100 ml

General

Gentamicin is an antibiotic of the aminoglycosidefamily that inhibits bacterial protein biosynthesis bybinding to the 30S subunit of the ribosome. It is activeagainst gram negative and gram positive bacteriaincluding mycoplasma.

Applications

> Effective against gram positive and gram negativebacteria

> Limited effectiveness against mycoplasma> Suitable on primary cells

Features

> Broad range antibiotic> Impedes protein biosynthesis


Recommended final concentration: . . . . . . 50 µg/ml


pH P11-004 . . . . . . . . . . . . . . 6.0 – 7.0P11-005 . . . . . . . . . . . . . . 4.5 – 5.5

Osmolality P11-004 . . . . . . . 0 – 50 mOsmol/kgP11-005 . . . . 100 – 200 mOsmol/kg

Endotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/ml


Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . 2 yearsStorage . . . . . . . . . . . . . . . . . . . . . +2°C to +8°CStability . . . . . . . . . . . . . . . . . . . 5 days at +37°C

Formulation

Gentamicin (P11-004). . . . . . . . . . . . . . . . . . . . . . . . . 10 mg/mlGentamicin (P11-005). . . . . . . . . . . . . . . . . . . . . . . . . 50 mg/ml

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Water

Cat. No. Volume

Penicillin/Streptomycin100x Concentrate P11-010 100 ml

Applications

> Effective for gram positive and gram negativebacteria

> Recommended as prophylaxis

Features

> Broad range antibiotic> Inhibition of protein biosynthesis (streptomycin)> Impedes cell wall synthesis (penicillin)







Formulation

Penicillin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10.000 Units/mlStreptomycin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 mg/ml

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.9 % NaCl


Reag

ents

MycoKill AB


Reag

ents

Cat. No. Volume

MycoKill AB50x Concentrate P11-016 100 ml

General

Mycoplasma, first discovered in 1898, were classifiedas viruses. There are more than 100 known speciesbelonging to the genus Mycoplasma. They are thesmallest free-living, self-replicating organisms and lacka cell wall so that antibiotics such as penicillin whichinterfere with murein formation are not effective. Ifnot treated mycoplasma contaminations can lead todiminished cell growth and to the loss of cultures. MycoKill AB is active at low concentrations for a broadrange of mycoplasma subspecies. It acts on the proteinsynthesis machinery by interfering with ribosome trans-lation as well as with mycoplasma RNA transcription.

Mycoplasma

> Effect cell growth> Modulate cytokine production of cells> Influence virus propagation in cell culture> Induce chromosomal aberrations in vitro> Effect DNA/RNA synthesis> Reduce metabolic activation

Detection of Mycoplasma

It is necessary to screen cell cultures periodically for apossible mycoplasma contamination. To ensure detec-tion it is recommended that different methods areused. The detection is necessary when working withestablished cell lines as well as with primary material.

Applications

> Mycoplasma infected established cell lines> Decontamination of primary material> Prophylaxis for cell culture

Features

> Effective mycoplasma removal> Effective against a broad range of mycoplasma> Does not negatively effect eukaryotic cell proliferation> Easy to use> Cost effective




Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . PBSpH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.0 – 4.5Osmolality . . . . . . . . . . . . . 280 – 340 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/ml

Cell Culture . . . . . . . . . . . . . . . . . . . . . . . . .testedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested


Phot

o: M

. Ro

hde,

GBF

, Br

auns

chw

eig;

C

. U

phof

f, D

SMZ,

Bra

unsc

hwei

g

Mycoplasma growing on the surface of infected host cell.

Accutase Alfazyme

Cat. No. Volume

Accutase L11-007 100 ml

Applications

> FACS analysis> Transfection> Toxicological tests> Plaque assays> Hapto taxis> Migration assays> Insect cell culture> Alternative to trypsin

Features

> Accutase has been developed to detach adherentcells gently and effectively

> Accutase combines proteolytic and collagenolyticactivities

> Accutase contains neither bacterial nor mammalianderived products

Advantages

> Ready to use> Protection of surface epitopes> Cell-cell recognition> Detaches adherent cells in minutes> Dissociates cells gently and rapidly> No neutralization necessary> No risk of cross-contamination


Solvent . . . . . . . . . . . . . . . PBS with 0.5 mM EDTApH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.8 – 7.8Osmolality . . . . . . . . . . . . . 270 – 320 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/ml

Cell Detachment . . . . . . . . . . . . . . . . . . . . testedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested


Cat. No. Volume

Alfazyme L11-012 100 ml

Applications

> Ultra gentle detacher for sensitive cells> Protection of surface epitopes> Alternative to Accutase

Features

> New formulation with a strong negatively chargedglucan polymer

> Mild acting enzymes with proteolytic and collageno-lytic activities

> Maximum viability of the cells> Improved stabilization of cells

Advantages

> Effective dissociation of most cell lines> Enzymatic and non-enzymatic components> Glucan polymer for dissociation and stabilization> No damage of surface antigens and receptors> No neutralization necessary> Particularly suitable for FACS analysis






Reag

ents


Reag

ents

AccuMax Collagenase 2

Cat. No. Volume

AccuMax L02-008 20 mlL11-008 100 ml

Applications

> Suitable for suspension cells, like hybridomas, CHO,BHK, Sf9 and 293 cells

> Separation of cell aggregates> Increase of the metabolism rate of suspension cells> Insect cell culture and hybridoma cell culture> FACS analysis> Cell counting

Activity

> Dependent on cell system> 0.5 ml to 2.0 ml per 10.0 ml suspension culture> 0.2 ml to 1.0 ml for cell pellets

Features

> AccuMax has been developed to dissociate cellclumps gently and rapidly

> AccuMax combines protease, collagenolytic andDNAse activities

> AccuMax contains neither bacterial nor mammalianderived products

Advantages

> Detaches clumps of suspension cells in culture > Dissociation of cell pellets> Accurate and reproducible cell counts> Higher protein expression





Cat. No. Volume

Collagenase 2 K21-240 1 g

Collagenase 2dialysed, sterile K02-239 20 ml

General

Collagenase cleaves peptide bonds in native, triple-helical collagen. The substrate, collagen, is the majorfibrous component of animal extracellular connectivetissue: skin, tendon, blood vessels, bone, etc.Collagenase may cleave simultaneously across all threechains or attack a single strand.

Application

Collagenase is suitable for the digestion of connectivetissues.

Features

> Most active collagenase> Collagenase 2 digests all native helical collagen fibrils> Important enzyme within the metabolism of tissues> Fixed on macroglobulin or anti-proteases during its

native condition> Digestion of polypeptides (gelatine)

Active components: collagenase, caseinase, clostripain.


Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . PBSConcentration . . . . . . . . . . . . . . . . . . . 2 Units/mlActivity (Wünsch) . . . . . . . . . . . . . ≥ 0.1 Units/mg(One unit catalyses the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucyl-glycyl-L-prolyl-D-arginine per minute at +25°C, pH 7.1)Source . . . . . . . . . . . . . . . Clostridium histolyticum

Cell Detachment . . . . . . . . . . . . . . . . . . . . tested

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . 2 yearsStorage Powder . . . . . . . . . . . . . . +2°C to +8°C

Solution . . . . . . . . . . . . . . . . . . ≤ −15°C

Trypsin (1:250) 10x Concentrate

Trypsin (1:250)1x Concentrate

Cat. No. Volume

Trypsin (1:250)10x Concentrate L11-001 100 ml

Applications

> Ready to use solution> Detachment of cells> Variable trypsin concentrations allow cell type

specific detachment procedures

Features

> Supports the detachment of cells effectively> Maintains cell viability





Powder:Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedVirus free . . . . . . . . tested for porcine parvovirusesActivity . . 1 g trypsin digests 250 g casein substrateSource . . . . . . . . . . . . . . . . . . . . . . . . . . . porcine

Formulation

Trypsin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 mg/ml

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . PBS, without Ca & Mg

Cat. No. Volume

Trypsin (1:250)1x Concentrate L11-002 100 ml

Applications

> Ready to use solution> Detachment of cells

Features

> Supports the detachment of cells effectively > Maintains cell viability






Formulation

Trypsin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.5 mg/ml



Reag

ents


Reag

ents

Trypsin EDTA (1:250) 10x Concentrate

Trypsin EDTA (1:250) 1x Concentrate

Cat. No. Volume

Trypsin EDTA (1:250)10x Concentrate L11-003 100 ml

Applications








Formulation

Trypsin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.0 mg/mlEDTA (Titriplex III) . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.2 mg/ml


Cat. No. Volume

Trypsin EDTA (1:250)1x Concentrate L11-004 100 ml

Applications







Formulation

Trypsin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.5 mg/ml EDTA (Titriplex III) . . . . . . . . . . . . . . . . . . . . . . . . . . 0.22 mg/ml



Reag

ents

Trypsin EDTA (1:250) UV Inactivated (10x)

Trypsin EDTA (1:250) UV Inactivated (1x)

Cat. No. Volume

Trypsin EDTA (1:250)UV Inactivated (10x) L11-659 100 ml

Applications



UV Inactivation

Trypsin is treated by a validated UV inactivation processto destroy viruses and mycoplasma. Any potential con-tamination is completely eliminated.This process has no effect on the enzymatic activity oftrypsin.






Formulation

Trypsin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.0 mg/ml EDTA (Titriplex III) . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.2 mg/ml


Cat. No. Volume

Trypsin EDTA (1:250)UV Inactivated (1x) L11-660 100 ml

Applications


UV Inactivation

Trypsin is treated by a validated UV inactivation processto destroy viruses and mycoplasma. Any potential con-tamination is completely eliminated.This process has no effect on the enzymatic activity oftrypsin.






Formulation

Trypsin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.5 mg/ml EDTA (Titriplex III) . . . . . . . . . . . . . . . . . . . . . . . . . . 0.22 mg/ml



Reag

ents

Trypsin (1:250) UV Inactivated (1x)

Cat. No. Volume

Trypsin (1:250)UV Inactivated (1x) L11-658 100 ml

Applications


UV Inactivation

Trypsin is treated by a validated UV inactivation processto destroy viruses and mycoplasma. Any potential con-tamination is completely eliminated. This process hasno effect on the enzymatic activity of trypsin.






Formulation

Trypsin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.5 mg/ml


Hybridoma Cloning Factor

Cat. No. Volume

Hybridoma Cloning Factor S05-015 50 ml

General

Hybridoma cells are created by fusion of an antibodyproducing cell with a tumour cell. Hybridoma cells canproduce monoclonal antibodies which are necessaryfor clinical diagnostics and human therapy. The Hybridoma Cloning Factor (HCF) is used as a sup-plement of the basal medium. HCF supplies specificgrowth factors needed for maximum growth of thehybridoma cells. It is needed for effective selection ofthe right clone, for antibody production and for thefreezing of hybridomas.

Manufacturing

HCF is prepared from a cultured murine macrophagelike cell line. The cell line constantly produces a varietyof growth factors for optimal hybridoma growth andcloning efficiency.

Applications

> Cloning of hybridoma cells> Hybridoma development> Selection of hybridoma clones> Antibody production> Thawing and freezing of hybridoma cells

Features

> High cloning efficiency> Improved growth after fusion> No preparation of feeder cells> Mixture of native growth factors> Optimized additive for hybridoma development> Ready to use solution


Recommended final concentration: . . . . . . . . . 10 %


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.9 − 7.6Endotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . value

Mycoplasma . . . . . . . . . . . . . . . . . . . not detectedSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested



Reag

ents

HematoStem

Cat. No. Volume

HematoStem U05-019 50 ml

General

Hematopoietic Stem Cells (HSC) are adult stem cellsand can be isolated from blood or bone marrow. Theyare able to replenish themselves continually and diffe-rentiate to a variety of specialized cells. They are res-ponsible for the constant renewal of erythrocytes, pla-telets, lymphocytes and myeloid cells. Hematopoieticstem cells have been studied for many years and werethe first stem cells to be used in therapies. HematoStem is a potent source of colony stimulatingfactors which are essential for the cultivation of hema-topoietic stem cells.

Applications

> Hematopoietic stem cells> Supports leukaemia cells> Cytogenetic analysis of bone marrow cells> Dendritic, endothelial and various tumour cells

Features

> Ready to use supplement> Increased productivity> No preparation of feeder cell layer required

Composition

> IMDM> 5 % FBS> Kanamycin Sulphate> Specific growth factors from a tumour cell line:

> Granulocyte macrophage colony-stimulating fac-tor (GM-CSF)

> Granulocyte colony-stimulating factor (G-CSF)> Macrophage colony-stimulating factor (M-CSF)> Interleukin-1 (IL-1)> Interleukin-6 (IL-6)> Plasminogen activator> Prostaglandin E


pH at 25°C . . . . . . . . . . . . . . . . . . . . . . . 6.9 – 7.6Endotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/ml




Reag

ents

B27 NeuroMix G5 Supplement

Cat. No. Volume

B27 NeuroMix 50x Concentrate F01-002 10 ml

General

B27 NeuroMix is a serum free supplement for long-termviability and growth of hippocampal and other neurons ofthe central nervous (CNS) and peripheral nervous system(PNS). The supplement is a chemically defined 50x con-centrate and contains vitamins, hormones and othergrowth factors including insulin, transferrin, catalase,antioxidants and fatty acids. When added to NeuronalBase Medium, B27 NeuroMix supports the growth ofneuronal cells without the need of specific feeder cells.

Applications

> Serum substitute> Growth of hippocampal neurons> Variety of CNS neurons> Supplement for Neuronal Base Medium

Features

> Different growth factors for neuronal cells> With specific antioxidants> With neuronal specific fatty acids

Composition

Proteins: . . . . . . . . . . . . . . . . . . . . Albumin, bovine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Catalase

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Insulin . . . . . . . . . . . . . . . . . . . . . . Superoxide Dismutase

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Transferrin


pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Endotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . value


Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . . 1 yearStorage . . . . . . . . . . . . . . . . . . . . . . . . . . ≤ −15°C

Cat. No. Volume

G5 Supplement 100x Concentrate F001-003 1 ml

General

G5 Supplement is a serum free chemically definedsupplement for cultivation of primary glial cells ortumour cells of astrocytic phenotype based onBottenstein´s G5 formulation.

Applications

> Serum free cultivation> Serum substitute> Growth of glial cells> Cultivation of tumour cells of astrocytic phenotype

Can be used in combination with: > Neuronal Base Medium> Neuronal Base Medium AD> DMEM, DMEM/Ham's F-12, MEM or other basal

media

Features

> With insulin, transferrin, EGF and FGF> Optimized hormone composition> With specific trace elements





Formulation µg/ml

Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100.00EGF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00FGF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.50Human Transferrin . . . . . . . . . . . . . . . . . . . . . . . . . . . . 500.00Hydrocortisone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.36Insulin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 500.00 Sodium Selenite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.52

BiotinL-CarnitineCholesterolCorticosteroneEthanolamineD(+)-GalactoseGlutathione (reduced)LecithinLinoleic AcidLinolenic Acid

PhosphatidylcholinProgesteronePutrescineRetinolRetinyl AcetateSodium SeleniteT3 (Triodo-l-Thyronine)DL-α-Tocopherol (Vitamin E)DL-α-Tocopherol Acetate


Reag

ents

N2 Supplement Neuronal Stem Cell Supplement

Cat. No. Volume

N2 Supplement 100x Concentrate F005-004 5 ml

General

N2 Supplement is a serum free, chemically definedsupplement based on Bottenstein's N2 formulation.This supplement is used for the cultivation of neuro-blastomas and neurons in primary cultures from boththe peripheral nervous system (PNS) and the centralnervous system (CNS).

Applications

> Serum free cultivation> Serum substitute> Growth of neuroblastomas> Ideal for a variety of CNS and PNS neurons > Used in combination with Neuronal Base Medium

Features

> Selected growth factors for neuronal tumour cells> Optimized hormone composition





Formulation µg/ml

Human Transferrin (holo) . . . . . . . . . . . . . . . . . . . . . . . 500.00Insulin (Bovine) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 500.00Progesterone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.63Putrescine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1611.00Sodium Selenite. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.52

Cat. No. Volume

Neuronal Stem Cell Supplement 50x Concentrate F01-005 10 ml

General

Neuronal Stem Cell Supplement is a serum free sup-plement for growth of neurospheres and central ner-vous system (CNS) progenitor stem cells. The supple-ment (50x concentrate) contains different vitamins,hormones and other growth factors such as insulin,transferrin, catalase, antioxidants, fatty acids and fattyacid precursors. When added to Neuronal BaseMedium, this supplement enhances the growth ofneuronal stem cells without the need of specific feedercells.

Applications

> Serum free cell culture of CNS progenitor and stem cells> In combination with Neuronal Base Medium

Features

> Different neuronal specific hormones> With specific growth factors for neuronal stem cells> Contains different antioxidants> Reduction of reactive oxygen damage

Composition

Proteins: . . . . . . . . . . . . . . . . . . . . Albumin, bovine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Catalase

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Insulin . . . . . . . . . . . . . . . . . . . . . . Superoxide Dismutase

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Transferrin





BiotinL-CarnitineCorticosteroneEthanolamineD(+)-GalactoseGlutathione (reduced)LecithinLinoleic Acid

Linolenic AcidPhosphatidylcholinProgesteronePutrescineSodium SeleniteT3 (Triodo-l-Thyronine)DL-α-Tocopherol (Vitamin E)DL-α-Tocopherol Acetate


Reag

ents

Nanofectin Kit

Cat. No. Volume

Nanofectin Kit Q050-005Nanofectin 0.5 mlDiluent 50 ml

Nanofectin Kit Q051-005Nanofectin 1.0 mlDiluent 50 ml

General

Different transfection methods are used for the transferof nucleic acids into mammalian cells. The transfectionefficiency depends on the size of the particle to betaken up by the cells and on the DNA stability. Nanofectin is a transfection reagent for optimal uptakeof nucleic acids into the cell which can be used in thepresence of serum. DNA stability is ensured as it isbound into the porous nanoparticles where it is pro-tected against endonucleolytic degradation.PAA provides Nanofectin as a kit system in combi-nation with an optimized diluent.

Applications

> Transfection reagent for mammalian cells> Transfection of DNA> Broad cell range reagent> Ideal for adherent and suspension cells> Successful for transfection of difficult cells> For transient and stable expression

Features

> Provided as kit system> Consists of a positively charged polymer and porous

nanobeads> Protection of bound DNA against endonucleolytic

degradation> Efficient binding of DNA> Uniform size for optimal uptake into the cell > Easy to use > High transfection rate> Transfection in presence of serum> Tested with several cell lines> Non toxic


Solvent Nanofectin . . . . . . . . . . . . . . . . . WaterDiluent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . NaCl Endotoxin . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/ml


Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . . 1 yearStorage . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C


Reag

ents

G-418 Sulphate

Cat. No. Volume

G-418 Sulphate P21-011 1 gP25-011 5 gP31-011 10 g

G-418 Sulphate Solution(50 mg/ml) P02-012 20 ml

P11-012 100 ml

General

G-418 is an aminoglycoside antibiotic that interfereswith the function of 80S ribosomes in protein synthe-sis in eukaryotic and prokaryotic cells. G-418 blockspolypeptide synthesis by binding irreversibly to 80Sribosomes and inhibiting the elongation step. It is usedas a selective antibiotic in molecular and cell biology.G-418 is usually more effective in multiplying cells thanin those which are not dividing. A multiplying cell willrequire 3 to 7 days for death to occur.

Applications

> Selection antibiotic > Neomycin transferase inactivates G-418> Transfections with the neomycin gene are resistant

to G-418> Transient and stable transfection

Features

> Antibiotic with a broad spectrum of activity> Toxic against all prokaryotes and eukaryotes> High purity> High activity> Packed in dark glass bottles> Sterile solution


Recommended final concentration: . . . . . . . . . . . . . . . . . . . . . . . .100 µg/ml – 1mg/ml


Activity (powder) . . . . . . . . . . . . . . .> 600 µg/mg

Endotoxin . . . . . . . . . . . . . . . . . . . . . . .< 10 EU/ml

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . .2 yearsStorage Powder . . . . . . . . . . . +15°C to +25°C

Solution . . . . . . . . . . . . . . . . . ≤ −15°C


Reag

ents

Puromycin Blasticidin

Cat. No. Volume

Puromycin P11-019 100 mgP15-019 500 mg

General

Puromycin is an aminonucleoside antibiotic used forselection of mammalian cell lines expressing the puro-mycin resistance gene. The antibiotic is active againstgram positive bacteria and various animal and insectcells by inhibiting the peptidyl transfer on prokaryoticand eukaryotic ribosomes. Transfected cells become resistant against puromycinby expression of the pac gene encoding a puromycinN-acetyl transferase.

Applications

> Selection antibiotic for transfection> Spectrum: gram positive bacteria, various animal

and insect cells> Selection of cell types harbouring plasmids carrying

puromycin resistance genes> Can be used as an alternative to the neomycin

system for transfection experiments

Features

> Aminoglycoside antibiotic> Inhibition of peptidyl transfer> High activity> High purity


Recommended final concentration: . . . 1 – 10 µg/ml


Purity . . . . . . . . . . . . . . . . . . . . . . . . . . > 98.0 %


Cat. No. Volume

Blasticidin P05-017 50 mgP11-017 100 mg

General

Blasticidin is a peptidyl nucleoside antibiotic used forselection of cells expressing the bsr gene. The anti-biotic is active against many bacteria, yeast and animalcells by interfering with the peptide bound formationin the ribosomal machinery of prokaryotes andeukaryotes. Resistance to blasticidin is conferred by theblasticidin resistence gene bsr which codes forblasticidin deaminase.

Applications

> Selection antibiotic used in transfection> Inactivation of blasticidin with acetyl transferase

and deaminases> Cells transfected with plasmids carrying the pac,

bls, bsr and bsd genes are resistant againstblasticidin

Features

> Nucleoside antibiotic> Inhibition of peptidyl transfer> Spectrum: bacteria, various animal cells and yeast> High purity> High activity


Recommended final concentration: . . . 3 – 50 µg/ml


Purity . . . . . . . . . . . . . . . . . . . . . . . . . . > 98.0 %



Reag

ents

Hygromycin B Carbenicillin

Cat. No. Volume

Hygromycin B P15-014 500 mgP21-014 1 g

Hygromycin B Solution(50 mg/ml) P02-015 20 ml

General

Hygromycin B is a unique aminoglycoside antibiotic pro-duced by Streptomyces hygroscopicus. It can be used asa selective antibiotic in molecular genetics and in cellculture experiments to inhibit the growth of prokaryotic(bacteria) and eukaryotic microorganisms (yeasts) as wellas mammalian cells. Hygromycin B inhibits the proteinsynthesis at the translocation step by interfering withthe 70S ribosome causing misreading of the mRNA. It iseffective on most bacteria, fungi and higher eukaryotes.

Applications

> Selection antibiotic > Hygromycin B phosphotransferase inactivates

Hygromycin B> Transfections with the hph gene are resistant to

Hygromycin B> Transient and /or stable transfected cells

Features

> Antibiotic with a broad spectrum of activity> Toxic against all prokaryotes and eukaryotes> High purity> High activity> Packed in dark glass bottles> Sterile solution


Recommended final concentration: . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 – 800 µg/ml


Solution:Osmolality . . . . . . . . . . . . . . 80 – 140 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/mlSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested

Powder:Purity . . . . . . . . . . . . . . . . . . . . . . . . . . > 85.0 %Potency . . . . . . . . . . . . . . . . . . . . . . . > 900 U/mg

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . 2 yearsStorage Solution . . . . . . . . . . . . . +2°C to +8°C

Powder . . . . . . . . . . . . . +2°C to +8°C

Note: Hygromycin B is light sensitive. Hygromycin B shouldbe protected from light during shipping and storage.

Cat. No. Volume

Carbenicillin P21-020 1 g P25-020 5 gP31-020 10 g

General

Carbenicillin belongs to the family of penicillin and isactive against gram negative and gram positive bacte-ria. It inhibits the crosslinking of peptidoglycan bybinding and inactivating transpeptidases and thereforeprevents bacterial cell wall synthesis. Carbenicillin issensitive to beta-lactamase and it is non toxic to plantcells.

Applications

> Selection antibiotic in transfection> Transformation of dicot plants (e.g. Nicotiana taba-

cum) by infection with Agrobacterium (Ti-plasmid)> Effective suppression of satellite colonies after trans-

formation of E. coli> Alternative to ampicillin> Cells transfected with plasmids carrying the bla gene

are resistant against Carbenicillin

Features

> Broad spectrum antibiotic> Carboxypenicillin antibiotic> Effective against gram negative and gram positive

bacteria especially Agrobacterium> High purity> High activity


Recommended final concentration: . . . . . . . . . . . . . . 50 – 300 µg/ml (Agrobacterium)


Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . WaterPurity . . . . . . . . . . . . . . . . . . . . . . . . . . > 92.0 %



Reag

ents

Lymphocytes Separation Medium LSM 1077

Cat. No. Volume

LSM 1077 J11-004 100 mlJ15-004 500 ml

Applications

> Isolation of lymphocytes from whole blood> Separation of monocytes and neutrophile cells> Separation of subcellular particles

Features

> Density gradient based on Ficoll 400, diatrizoic acidand sodium hydroxide

> Optimized separation solution with a density of1.077 g/ml

> Physiological parameters> High density at low viscosity> Sterile ready to use solution


Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . WaterpH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0 – 7.5Osmolality . . . . . . . . . . . . . 240 – 320 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 4 EU/ml


Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . 2 yearsStorage . . . . . . . . . . . . . . . . . . . +15°C to +25°C

Note:The Lymphocyte Separation Medium LSM 1077 is lightsensitive. The medium should be protected from lightduring shipping and storage.


Reag

ents

Bovine Serum Albumin Fraction V

Bovine Serum AlbuminFatty Acid Free

Cat. No. Volume

Bovine Serum AlbuminFraction V K41-001 100 g

K45-001 500 g

Applications

> Serum free cell culture> Additive for hybridoma cell culture> Serum standard> Protein stabilizer> Blocking agent

Features

> High purity> Neutral pH-value> Best standard in biological assays> No IgG


Protein . . . . . . . . . . . . . . . . . . . . . . . . . ≥ 96.0 %Albumin . . . . . . . . . . . . . . . . . . . . . . . . ≥ 98.0 %Moisture . . . . . . . . . . . . . . . . . . . . . . . . . < 3.0 %

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . 5 yearsStorage . . . . . . . . . . . . . . . . . . . . +2°C to +25°C . . . . . . . . . . . . . . . . . . . . . . .in cool, dry conditions

Cat. No. Volume

Bovine Serum AlbuminFatty Acid Free K31-002 10 g

K35-002 50 g

Applications

> Stabilizer / enhancer > Additive for cell culture> "Binding and transport” studies> Suitable for diagnostic systems

Features

> High purity> Extremely low level of total lipids and fatty acids> No IgG> Neutral pH


Protein . . . . . . . . . . . . . . . . . . . . . . . . . ≥ 96.0 %Purity . . . . . . . . . . . . . . . . . . . . . . . . . . ≥ 98.0 %Moisture . . . . . . . . . . . . . . . . . . . . . . . . . < 3.0 %



Reag

ents

Bovine Serum Albumin30% Solution

Bovine Serum AlbuminLow Endotoxin

Cat. No. Volume

Bovine Serum Albumin30% Solution K05-013 50 ml

K11-013 100 ml

Applications

> Serum free cell culture> Additive for hybridoma cell culture> Serum standard> Protein stabilizer> Coating reagent> Rhesus compatibility tests> Stabilization of platelets

Features

> Very high purity> Optimum pH-value> No T-cell-activation> Ready to use solution> Gamma irradiated> Without Na-azide


Protein . . . . . . . . . . . . . . . . . . . . . . . . . > 30.0 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.5 – 7.2


Cat. No. Volume

Bovine Serum AlbuminLow Endotoxin K31-011 10 g

K35-011 50 g

Applications

> Serum free cell culture> Additive for hybridoma cell culture> Stabilizer> Diagnostic assays

Features

> High purity> Low endotoxin content> No IgG> Only slight batch to batch variations


Protein . . . . . . . . . . . . . . . . . . . . . . . . . ≥ 96.0 %Albumin . . . . . . . . . . . . . . . . . . . . . . . . ≥ 98.0 %Moisture . . . . . . . . . . . . . . . . . . . . . . . . . < 3.0 %Endotoxin . . . . . . . . . . . . . . . . . . . . . . < 5 EU/mg



Reag

ents

Sodium Pyruvate

Cat. No. Volume

Sodium PyruvateSolution (100 mM) S11-003 100 ml

General

Sodium Pyruvate can be added to cell culture media asan additional, easily accessible carbohydrate source incultivation of cells. It is involved in the production ofATP within the cell. Especially for rapid growing cellslike some cancer cell lines additional sodium pyruvatecan be added to the growth medium.

Applications

> Additive for media> Additional energy source for cells> For strong growing cells

Features

> Carbon source for metabolism> Sterile stock solution





Formulation

Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.0 g/l

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Water

Bovine Serum AlbuminProtease Free

Cat. No. Volume

Bovine Serum AlbuminProtease Free K41-012 100 g

K45-012 500 g

Applications

> Immuno diagnostic assays> Blocking protein in ELISA systems> Stabilizer of enzyme activities> Background protein> Protein stabilizer> Hybridisation techniques

Features

> Contains no residual protease activity by caseinhydrolysate assay

> Protects sensitive protein components> Reduces non-specific binding of protein> Reduces or eliminates background interference in

ELISA> Contains no IgG


Protein . . . . . . . . . . . . . . . . . . . . . . . . . ≥ 96.0 %Albumin . . . . . . . . . . . . . . . . . . . . . . . . ≥ 98.0 %Moisture . . . . . . . . . . . . . . . . . . . . . . . . . < 3.0 %

Protease . . . . . . . . . . . . . . . . . . . . . . not detected



Reag

ents

Giemsa Stain Solution Potassium Chloride

Cat. No. Volume

Giemsa Stain Solution S11-019 100 ml

General

Giemsa stain solution is a DNA stain that consists of amixture of dyes including the basic aminophenothia-zine dye. Giemsa staining allows different chromoso-mes to be distinguished by their specific pattern.

Application

> Stain for G-banding of chromosomes


Recommended final concentration: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5ml/10ml (PBS)


Shelf Life . . . . . . . . . . . . . . . . . . 2 years (unopen)Storage . . . . . . . . . . . . . . . . . . . +15°C to +25°CStability . . . The diluted solution is stable for 1 day

Cat. No. Volume

Potassium Chloride(0.075 M) S11-018 100 ml

Applications

> Hypotonic solution to enlarge cells for adequatespreading of metaphase chromosomes

> For the preparation of blood lymphocyte chromosomes



Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . 3 yearsStorage . . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Formulation

Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.075 M

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Water


Reag

ents

Colcemid Phytohaemagglutinin (PHA)

Cat. No. Volume

Colcemid J01-003 10 ml

General

N-deacetyl-N-Methylcolchicine (Colcemid) is related tocolchicine but it is less toxic. Colcemid depolymerisesmicrotubules and limits microtubule formation.Appropriate dilutions inactivate the spindle fibremechanism during metaphase. Colcemid is used inchromosome analysis during lymphocyte karyotypingand in amniotic fluid cell chromosome analysis.

Applications

> Arrests cells in metaphase> For chromosome analysis during lymphocyte karyo-

typing> For amniotic fluid cell chromosome analysis


Recommended final concentration: . . . . . 0.1 µg/ml




Hazard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . toxic

Note:Colcemid is light sensitive. It should be protected fromlight during shipping and storage.

Formulation

Colcemid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 µg/ml

Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . HBSS

Cat. No. Volume

Phytohaemagglutinin (PHA) J01-006 10 ml

General

Phytohaemagglutinin (PHA) is a lectin extracted fromred kidney beans (Phaseolus vulgaris). It has a numberof physiological effects and is used in medical research.It induces mitosis and effects the transport of proteinsacross the cell membrane. PHA is used for the stimula-tion of cell proliferation in lymphocyte cultures. In highdoses PHA is a toxin.Since PHA is subjected to batch variations PAA offers asterile, ready to use solution with a defined concentra-tion.

Application

> For the stimulation of cell proliferation in lymphocy-te cell cultures

Features

> Ready to use> Defined mitogenic activity


Recommended final concentration: . . . . . . . . . 10 %


Solvent . . . . . . . . . . . . . . . . . . . . . . . 0.9 % NaCl

Lymphocyte cell growth . . . . . . . . . . . . . > 45 %Chromosome morphology . . . . . . . . . . . . positiveSterility . . . . . . . . . . . . . . . . . . . . . . . . . . . tested



Reag

ents

CryoMaxx

Cat. No. Volume

CryoMaxx I J05-011 50 mlCryoMaxx II J05-012 50 mlCryoMaxx SF J05-010 50 mlCryoMaxx S J05-013 50 ml

General

Cryopreservation is a procedure to stabilize cells atcryogenic temperatures for long term storage. Cellscontain fragile membranes and organelles which canbe destroyed by ice formation during the freezing pro-cedure. The use of cryoprotective additives or chemi-cals protects the cells during the freezing process byminimizing the damage associated with ice crystalformation.

Serum free Cryopreservation Media

CryoMaxx I is a serum free cryoprotective mediumdeveloped especially for DMSO sensitive cells. The lowDMSO concentration (5 %) and different new cellstabilizers provide best viability after thawing.

CryoMaxx II is a serum free cryoprotective mediumalso based on organ preservation media with a higherDMSO concentration (10 %). CryoMaxx I and II areused for cell lines adversely affected by prolonged con-tact with DMSO. CryoMaxx I and II are developed forserum free cell banking and therefore qualified for theuse in the biopharmaceutical industry.

CyroMaxx SF is a serum free cryoprotective mediumcontaining 10 % DMSO and methylcellulose.Methylcellulose is the main component for cell stabili-sation. CryoMaxx SF can also be used in preparation ofcell banking.

Serum containing Cryopreservation Media

CryoMaxx S is a classical serum containing cryopro-tective medium that contains 10% DMSO. The FBSused is extensively tested to protect cells during cellpreservation.

Composition

The cryopreservation media contain DMSO (5 – 10 %)as active substance. DMSO avoids destructive ice for-mation during freezing procedure. For cell stabilizationfurther components must be added. CryoMaxx I (with5% DMSO) and CryoMaxx II (with 10% DMSO) consistof a basal medium with different saccharides, reducingagents and colloids. CryoMaxx SF is based onDMEM/Ham's F-12 supplemented with methylcelluloseand 10 % DMSO. CryoMaxx S is a classical serum con-taining cryopreservation medium based onDMEM/Ham's F-12 with 20 % FBS and 10 % DMSO.

Applications

> Optimized preservation media> Cryopreservation of a wide variety of cell types> For cell banking> Storage of cells in liquid nitrogen

Features

> Ready to use solution> Contains cryoprotective agents to minimize dehy-

dration effects > Serum free and serum containing cryoprotective

media> Simple freezing protocol> Improved viability of cells after thawing

Storage

Shelf Life J05-010 to J05-012 . . . . . . . . . . 1 yearJ05-013 . . . . . . . . . . . . . . . . . . 2 years

Storage J05-010 to J05-012 . . . . +2°C to +8°CJ05-013 . . . . . . . . . . . . . . . . ≤ −15°C

Note:CryoMaxx is a light sensitive solution. It should beprotected from direct light during shipping and storage.


Reag

ents

CoolStar

Cat. No. Volume

CoolStar J11-007 100 ml

CoolStar Kit J12-078CoolStar 100 ml CoolStar Rinse 100 ml

General

Hypothermic preservation is a procedure to stabilizecells and organs at +2°C to +8°C. The goal of coldstorage is to induce hypothermia in order to suppressthe rate of cell deterioration. An optimal preservationsolution contains components to reduce cell swelling(colloids), buffer to maintain pH balance, antioxidantsto scavenge oxygen free radicals and adenosinetriphosphate precursors to provide energy upon reper-fusion. The cold storage of tissues and organs withoptimized hypothermic preservation media is a pre-requisite for successful animal organ transplantationexperiments.

CoolStar

CoolStar has been formulated to decrease the freeradical accumulation in tissues and organs. Free radicalaccumulation leads to cell death in the case of hypo-thermic preservation.

CoolStar Rinse has been formulated as a flush solutionto rinse culture media and native fluids from mamma-lian cells, tissues and organs. It should be used in com-bination with CoolStar.

Composition

CoolStar is a new hypothermic medium consisting ofsome disaccharides (sucrose, mannitol, and glucose),lactobionate, dextran, buffers and other reducingagents which reduce free radicals. CoolStar Rinse is asolution for rinsing of organs and tissues which con-sists of different disaccharides and buffers.

Applications

> Optimized medium for hypothermic preservation> Preservation of different mammalian cells, tissues

and organs

Features

> Ready to use solution> Simple protocol> Reduced viscosity> Gentle cold storage > Transportation of cells> Storage of cells and organs at +2°C to +8°C> Improved viability of cells after 2 – 5 days storage at

+2°C to +8°C

Storage

Shelf Life . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 yearStorage . . . . . . . . . . . . . . . . . . . . . . +2°C to +8°C

Note:CoolStar is a light sensitive solution. It should beprotected from direct light during shipping and storage.


Buffer Solutions

Buff

er S

olut

ions

Basal Salt Solutions 100

Dulbecco's Phosphate Buffered Saline (DPBS) . 100Hanks' Balanced Salt Solution (HBSS) . . . . . . 101Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . 102HEPES Buffer . . . . . . . . . . . . . . . . . . . . . . . . 102

Rinsing Solution 103

Cool Star Rinse . . . . . . . . . . . . . . . . . . . . . . 103

Water 103

Water, AP Grade . . . . . . . . . . . . . . . . . . . . . 103


Buff

er S

olut

ions

Buffer SolutionsBuffers are mainly used as rinsing and diluting solu-tions. PAA guarantees a constant high quality byusing highly purified salts and water.


Buff

er S

olut

ions

Salt solutions are used in many different applicationswithin research, diagnostic and industrial areas. PAAuses only prescreened high quality raw materials andwater in the manufacture of all salt solutions. Sterilebuffers, rinsing solutions and water are offered in avariety of convenient pack sizes.

Raw Material

All chemicals and raw materials are subject to stringentQuality Control and Quality Assurance (QC/QA) controlsbefore being released into the buffer production area.Only those raw materials which pass PAA's qualityspecifications are selected for use.

Water Quality

High quality water is a critical component of all buffersolutions. PAA uses purified water in the production ofall buffer solutions. The use of high quality water toget-her with QC controlled raw materials guarantees thatour customers only receive buffers of the highest quality.

Manufacturing

All buffer solutions are manufactured using carefullycontrolled Standard Operating Procedures (SOP's). Allproduction processes are carefully monitored to ensu-re product integrity. Sterile filtration through 0.2 µmfilter systems is performed. Finished buffer solutionsare fully QC tested and parameters such as endotoxinand osmolality are recorded on the correspondingCertificate of Analysis (CoA).

Packaging and Storage

The buffer solutions are supplied in a variety ofconvenient laboratory pack sizes in PET bottles.Larger volumes are supplied in 10 litre plastic con-tainers fitted with outlet taps.

PAA can also supply buffers in custom requested packsizes. For further information please refer to the‘customized products’ section in this catalogue.

All buffers are shipped at ambient temperature andshould be stored at room temperature upon arrival.

Introduction

Dulbecco's Phosphate Buffered Saline (DPBS)

Cat. No. Volume

Dulbecco's PBS (1x)with Ca & Mg H15-001 500 ml

Dulbecco's PBS (1x)without Ca & Mg H15-002 500 ml



Dulbecco's PBS (1x)without Ca & Mg H31-002 10 Litressterile container with tap

Applications

> Cell culture> Washing procedures> Dilution> Reference solution

Use

> Nutrient, transportation or dilution liquid for mainte-nance of the intra- and extracellular osmotic balance

> Provides cells with water and inorganic ions, whichare necessary for the cell metabolism

> Buffering system for cells outside of a nutrientmedium

> Stabilises cells during subculture

Product Specifications (1x)




Product Specifications (10x)

pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.5 – 7.0Osmolality . . . . . . . . . . . 2000 – 3000 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 5 EU/ml



Formulation g/l

(1x) (10x)Inorganic SaltsKCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.2 . . . . . . . . . 2.0 KH2PO4 . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.2 . . . . . . . . . 2.0NaCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.0 . . . . . . . . 80.0 Na2HPO4 anhydrous . . . . . . . . . . . . . . . . . 1.15 . . . . . . . 11.50CaCl2 • 2H2O in H15-001 . . . . . . . . . . . . 0.132MgCl2 • 2H2O in H15-001 . . . . . . . . . . . . . 0.1


Buff

er S

olut

ions

Hanks' Balanced Salt Solution (HBSS)


Buff

er S

olut

ions

Cat. No. Volume

Hanks' BSS (1x)with Ca & Mgwith Phenol Red H15-007 500 ml

Hanks' BSS (1x)with Ca & Mgwithout Phenol Red H15-008 500 ml

Hanks' BSS (1x)without Ca & Mgwithout Phenol Red H15-009 500 ml

Hanks' BSS (1x)without Ca & Mgwith Phenol Red H15-010 500 ml

Applications

> Cell culture> Washing procedures> Dilution> Reference solution

Use

> Nutrient, transportation or dilution liquid for mainte-nance of the intra- and extracellular osmotic balance

> Provides cells with water and inorganic ions, whichare necessary for the cell metabolism

> Buffering system for cells outside of a nutrientmedium

> Stabilises cells during subculture





Formulation g/l

Inorganic SaltsCaCl2 anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.14 KCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.40KH2PO4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.06 MgSO4 anhydrous. . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.09767NaHCO3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.35NaCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.00Na2HPO4 anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . . 0.04788

Other ComponentsD-Glucose anhydrous . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.00Phenol Red in H15-007, H15-010 . . . . . . . . . . . . . . . . . . . 0.01

Sodium Bicarbonate HEPES

Cat. No. Volume

Sodium Bicarbonate S11-002 100 ml

General

Cell growth leads to an increasing CO2 level. In theabsence of any buffer systems the medium becomesacidic. To maintain the physiological pH (7.2 – 7.4) ofa culture sodium bicarbonate is most commonly usedin combination with carbon dioxide as a bufferingsystem. Sodium Bicarbonate is also an essential growthsupplement.

Applications

> Biological buffer> Maintains physiological pH (7.2 – 7.4)

Features

> Less expensive than other buffers> Low toxicity> Nutritional value> Sterile stock solution


Concentration . . . . . . . . . . . . . . . . . . . . . . 7.5 %pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.5 – 8.5Osmolality . . . . . . . . . . . 1300 – 2100 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/ml



Cat. No. Volume

HEPES Buffer S11-001 100 ml

Applications

> For cells where sodium bicarbonate has a toxic effect> For the reduction of CO2 concentration in the incu-

bator

Features

> Dipolar ionic buffer> Maintains the pH in a range of 6.7 – 8.6> Sterile stock solution


Recommended final concentration . . . . .10 – 40 mM


Concentration . . . . . . . . . . . . . . . . . . . . . . . . 1 MpH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.5 – 7.5Osmolality . . . . . . . . . . . 1300 – 1500 mOsmol/kgEndotoxin . . . . . . . . . . . . . . . . . . . . . . < 10 EU/ml




Buff

er S

olut

ions


Buff

er S

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ions

CoolStar Rinse Water

Cat. No. Volume

CoolStar Rinse J11-008 100 ml

CoolStar Kit J12-078CoolStar 100 mlCoolStar Rinse 100 ml

General

Hypothermic preservation is a procedure to stabilizecells and organs at +2°C to +8°C. The goal of coldstorage is to induce hypothermia in order to suppressthe rate of cell deterioration. The cold storage oftissues and organs with optimized hypothermic preser-vation media is a prerequisite for successful animalorgan transplantation experiments.

CoolStar

CoolStar Rinse has been formulated as a rinsing solu-tion to flush the culture media and native fluids fromthe mammalian cells, tissues and organs. It should beused in combination with CoolStar. Both products areavailable in the convenient CoolStar Kit.

Composition

CoolStar Rinse is a solution for rinsing organs and tissueswhich consists of different disaccharides and buffers.

Application

> Washing solution

Storage


Note:CoolStar Rinse is a light sensitive solution. It should beprotected from direct light during shipping and storage.

Cat. No. Volume

WaterAP Grade, sterile S15-012 500 ml

S21-012 1000 mlContainer with tap S31-012 10 Litres

Applications

> Cell culture and molecular biology> Dilution purposes> Reference solution> Manufacture of media> Manufacture of reagents for cell culture

Aqua purificata (AP Grade)

Purified water can be prepared by distillation, ionexchange, by reverse osmosis or by any other suitablemethod that complies with the regulations on waterintended for human consumption. PAA water is produced by ion exchange and reverseosmosis. During production appropriate measures aretaken to ensure that the total viable aerobic count isadequately controlled and monitored. A further testparameter is the conductivity factor which describes theability of an aqueous solution to carry an electric cur-rent. A nutrient-rich solution will have a higher conduc-tivity than a solution with less ionic salts (nutrients). Thehigher the nutrient level the higher the conductivity.


Conductivity Factor (CF) . . . . . . . . . . < 20 µS/cmEndotoxin . . . . . . . . . . . . . . . . . . . . . . . < 1 EU/ml




Cytogenetics

Cyt

ogen

etic

s

Cytogenetic Media

Quantum 3-21 . . . . . . . . . . . . . . . . . . . . . . 108Quantum PBL . . . . . . . . . . . . . . . . . . . . . . . 109Ham's F-10 Ready Mix . . . . . . . . . . . . . . . . . 110

Serum

FBS Amnion cell tested . . . . . . . . . . . . . . . . 111

Reagents

Colcemid . . . . . . . . . . . . . . . . . . . . . . . . . . 112Potassium Chloride . . . . . . . . . . . . . . . . . . . 112Giemsa Stain Solution . . . . . . . . . . . . . . . . . 112Phytohaemagglutinin (PHA) . . . . . . . . . . . . . 112


Cyt

ogen

etic

s

CytogeneticsCytogenetics is the study of normal and abnormalchromosomes. Therefore PAA offers a new productline including special media, serum and reagentsfor the cultivation of amniocytes, chorioncytes andlymphocytes.


Cyt

ogen

etic

s

Cytogenetics is the study of chromosomes and therelated diseases caused by numerical and structuralchromosome abnormalities. Chromosome studies areimportant laboratory diagnostic procedures in prenataland postnatal diagnosis. The results of the diagnosisprovide important information for further medicalinterventions.

Chromosome studies can either be performed with theclassical staining method or with molecular methods.The classical cytogenetic analysis is based on thebanding pattern of dyed chromosomes. Thereforeseveral banding techniques have been developed (e.g.Q-, C- or G-banding). Using molecular cytogeneticsgenetic diseases based on mutations, small deletionsor insertions can be detected with Fluorescent In SituHybridization (FISH), Comparative Genomic Hybri-dization (CGH), PCR or DNA-Sequencing.

Karyotyping for classical chromosomal Analysis

For karyotyping metaphase chromosomes are preparedby subsequent cultivation in nutrient media andsynchronisation of cells in metaphase with inhibitors(colcemid). The proliferation of non dividing blood cellssuch as lymphocytes in culture, is stimulated by themitogen phytohaemagglutinin, providing a very acces-sible source of metaphase cells. Precise karyotypingallows the cytogeneticist to identify chromosomeabnormalities from amniotic fluid or foetal tissuesamples.

Cytogenetic products for In Vitro Diagnostics

Products used in In Vitro Diagnostics (IVD) are sub-jected to guidelines according to the directive98/79/EC defined by the European commission on27.10.1998. Several requirements like traceability,quality assurance, documentations, specific labellingand the declaration of conformity must be imple-mented into manufacturers total quality assurancesystem to ensure constant product quality. Since 7December 2003 products used for IVD must be label-led clearly with appropriate special characters and sym-bols (e.g. or ).

Prenatal Diagnostics

For detection of genetic diseases karyotyping is animportant procedure in prenatal diagnostics. Manydiseases and birth defects are a direct result of missing,broken, or extra chromosomes. Amniocentesis andChorionic Villus Sampling (CVS) are the most important

prenatal diagnostic tests. They both involve culturingof isolated foetal cells in specific nutrient media enri-ched with growth factors and hormones, followed bychromosome analysis to detect chromosomal abnor-malities.

Postnatal Diagnostics

In contrast to prenatal diagnostics where only limitedamounts of foetal cells can be used the postnatal dia-gnostics have access to blood cells in sufficient quantityto prepare a karyotype. Since lymphocytes are notactively dividing, cell mitosis must be induced with themitogen phytohaemagglutinin. For cytogenetic analy-sis proliferating lymphocytes of the peripheral bloodare cultivated in specific media and intact metaphasechromosomes are prepared with classical or molecularcytogenetic methods.

PAA´s complete Media

In the field of prenatal diagnostics, PAA can offerQUANTUM 3-21 and Ham´s F-10 Ready Mix, twocomplete media for quick and reliable diagnosis ofchromosomal abnormalities.

For postnatal diagnostics we offer QUANTUM PBL, ourready to use medium with phytohaemagglutinin forculturing peripheral blood lymphocytes.

With QUANTUM 3-21 and QUANTUM PBL, PAA offerstwo "CE marked” media for IVD which fulfil the requi-rements of the directive 98/79/EC defined by theEuropean commission.

Extensive quality control Tests

Extensive quality control tests assure constant highproduct quality. PAA manufactures cell culture pro-ducts according to current GMP. Further quality controland quality assurance are carried out to the higheststandards as required by the biopharmaceuticalindustry.

Introduction

For further details and specifications see page 30.


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QUANTUM 3-21

General

Amniocentesis and Chorionic Villus Sampling (CVS) areimportant prenatal diagnostic tests. They both involveculturing of isolated foetal cells, followed by chromo-some analysis to detect chromosomal abnormalities.

Amniocentesis is usually performed between 14 and20 weeks of the pregnancy. Cells from the fluid areexamined for signs of chromosomal abnormalities,defective enzymes or deficient growth. More than 75abnormalities can be detected by analyzing amnioticfluid cells.

Chorionic Villus Sampling (CVS) can be performedbetween 9.5 and 12.5 weeks gestation. This test isdone during pregnancy to detect chromosomal abnor-malities and inherited diseases. The most common testemployed on cells obtained by CVS is chromosomeanalysis to determine the karyotype of the foetus.

For growth of amniocytes and chorionic villi cells the useof pre-tested complete media is absolutely essential.

QUANTUM 3-21 for selective and fast Growth

PAA in cooperation with a number of institutes forhuman genetics has developed a complete medium forin vitro cultivation of amniocytes and chorionic villicells. By adding different hormones and novel growthfactors, considerable improvements in the growth cha-racteristics of amniocytes and chorionic villi cells havebeen achieved. Only selected batches of FBS fulfil therequirements of cultivation of amniocytes and chorio-nic villi cells. Batches used for PAA´s QUANTUM 3-21are determined by extensive tests.

This novel medium recipe enables rapid growth whilesimultaneously inhibiting other cell types. It is testedregularly by two independent cytogenetic laboratoriesfor its capability to support the growth of primaryamniocytes and chorionic villi cells.

With QUANTUM 3-21, clear karyograms for the iden-tification of chromosomal abnormalities can be drawn.

Composition

QUANTUM 3-21 consists of a modified Alpha-MEMsupplemented with pre-tested FBS, hormones, growthfactors, L-glutamine and gentamicin.

Features and Benefits

> Ready to use medium

> Improved growth

> Chromosome preparations

> Rapid and clear results

> Cell harvesting between 7-10 days

> Unlimited availability

Order information

U11-020 QUANTUM 3-21 . . . . . . . . . . 100 ml



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QUANTUM PBL

General

QUANTUM PBL is a complete medium stimulating thegrowth of peripheral blood lymphocytes used for pre-paration of intact chromosomes. It contains the mitogenphytohaemagglutinin (PHA) as the growth deter-mining factor. Blood cell karyotyping of lymphocytes is an importanttool in modern human cytogenetics to detect chromo-somal aberrations. For cytogenetic analysis peripheralblood lymphocytes are cultivated in specific media andintact chromosomes are prepared. Lymphocytes aremature cells that do not normally undergo subsequentcell divisions. In the presence of a mitogen lympho-cytes are stimulated to enter into mitosis by DNA repli-cation. After 48-72 hours, a mitotic inhibitor is addedto the culture to stop mitosis in the metaphase stage.After treatment with hypotonic solution, fixation andstaining, chromosomes can be microscopically observedand evaluated for abnormalities. For short-term cultureof lymphocytes the use of a mitogen to induce mitosisin non-dividing cells is required. The most commonlyused mitogen is phytohaemagglutinin (PHA) which is ared kidney bean extract that transforms lymphocytes. Bytesting the phytohaemagglutinin in cytogenetic labora-tories the PHA concentration is always optimized forbest stimulation of lymphocytes.

QUANTUM PBL for culturing Lymphocytes

QUANTUM PBL is PAA´s complete medium for theselective growth of peripheral blood lymphocytes. Itconsists of a modified base medium containing highquality phytohaemagglutinin (PHA), pre-tested FBS, L-glutamine and antibiotics.With QUANTUM PBL a large proportion of lymphocytesare stimulated for growth. Adequate cells are thereforeavailable for further preparation of intact chromosomesfor karyotyping. Consequently, clear karyograms todetect chromosome abnormalities can be drawn.

Quality Standards

Extensive quality control tests assure constant highproduct quality. PAA manufactures cell culture pro-ducts according to current GMP. Further quality controland quality assurances are carried out to the higheststandards as required by the biopharmaceuticalindustry. Every batch of Quantum PBL is tested by anindependent cytogenetic laboratory for its capacity tostimulate the growth of peripheral blood lymphocytes.The resulting karyograms are compared with referencestandards.


> Ready to use medium with pre-tested FBS

> Suitable for heparinized blood and lymphocyteenriched blood

> Improved growth

> Optimized PHA concentration

> Clear results

> Manufactured according to cGMP

Order information

U11-022 QUANTUM PBL . . . . . . . . . . . 100 ml



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Ham's F-10 Ready Mix

Chromosomal Analysis

The karyotyping of chromosomes isolated fromlymphocytes, amniocytes and chorionic villi cells is animportant tool in cytogenetics. For chromosomal ana-lysis peripheral blood lymphocytes and amniocytes arecultivated in media specifically designed to maximizethe yield of intact chromosomes.

Cultivation of Amniocytes and Chorionic VilliCells or Lymphocytes

Ham's F-10 is one of the most frequently used culturemedia in cytogenetics. To cultivate amniocytes andchorionic villi cells, important supplements such as foe-tal bovine serum (FBS) and antibiotics need to beadded. However, if you prepare your own media, aconsiderable amount of time is spent on testing theFBS. Ham's F-10 Ready Mix is a ready to use mediumconsisting of Ham's F-10 basal medium, FBS (pre-tested on amniocytes and chorionic villi cells), L-glutamine and gentamicin. It is a cost-effective alter-native to other special media used in cytogenetic labo-ratories. This complete medium, when supplementedwith phytohaemagglutinin (PHA), is also suitable forthe rapid growth of peripheral blood lymphocytes.

FBS pre-tested on Amniocytes and Chorionic Villi Cells

The reliability, safety and quality assurance testing ofFBS is performed in two independent cytogenetic labo-ratories. Primary amnion cell growth is screened andthe performance is compared to a reference standard.

Quality control Tests

Extensive quality control tests assure constant highproduct quality. PAA manufactures cell culture pro-ducts according to current GMP. Further quality controland quality assurances are carried out to the higheststandards as required by the biopharmaceuticalindustry.

Features


> Contains pre-tested FBS

> Suitable for growing amniocytes and chorionic villicells as well as lymphocytes

> Reduced workload and costs

> Optimized reproducibility

> For chromosome analysis

> For karyotyping

> For further analysis including FISH analysis

> Manufactured according to cGMP

Order information

R11-821 Ham's F-10 Ready Mix . . . . . . 100 ml



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Foetal Bovine SerumAmnion cell tested

Pre-tested on Amniocytes and Chorionic Villi Cells

Foetal Bovine Serum (FBS), as a source of specificgrowth factors, is necessary for cell cultivation.However, only specially selected sera batches meet therequirements for amniocytes and chorionic villi cellculture. PAA determines in cooperation with indepen-dent cytogenetic laboratories which batches are suita-ble by carrying out extensive tests, namely by:

> Quantitative growth assays on primary cultures ofamniocytes and chorionic villi cells

> Determination of growth rates

> Producing karyograms after preparing metaphasechromosomes

> Monitoring results against reference standards

Extensive batch-specific quality control testing guaran-tees constant quality as well as excellent growth cha-racteristics. PAA manufactures cell culture productsaccording to current GMP. Further quality control andquality assurance are carried out to the highest stan-dards as required by the biopharmaceutical industry.

Features

> Cultivation of amniocytes and chorionic villi cells

> Cultivation of lymphocytes

> Karyotyping

> Further analysis e.g. Fluorescent In Situ Hybridisation(FISH)

Order information

A11-110 FBS Amnion cell tested . . . . . . 100 mlA15-110 FBS Amnion cell tested . . . . . . 500 ml

See also

page 94page 93page 93

Order information

J01-006 Phytohaemagglutinin . . . . . . . . 10 ml


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Additional cytogenetic Products Phytohaemagglutinin

Colcemid

Colcemid arrests mitotic cultured cells in their meta-phase. It is recommended for use in chromosome ana-lysis during lymphocyte karyotyping and amnion fluidcell chromosome analysis. Care should be taken whenusing this solution (see material safety data sheet).

Potassium Chloride

Potassium chloride (0.075 M) is a hypotonic solutionused for the preparation of blood lymphocyte chromo-somes. The hypotonic treatment causes the cells toswell and aids in the release of intact chromosomes.

Giemsa Stain Solution

Giemsa is a complex of stains specific for the phos-phate groups of DNA. It is used in Giemsa banding (orG-banding) to stain chromosomes and to create akaryotype to identify chromosomal aberrations.

Stimulation of Lymphocytes

Normally lymphocytes from the peripheral blood arenon-dividing cells that have to be stimulated by themost commonly used mitogen phytohaemagglutinin(PHA), a lectin extract from red kidney beans(Phaseolus vulgaris). The protein consists of two mole-cular species, a leucoagglutinin (PHA-L) and anerythroagglutinin (PHA-E). Both species include afamily of five isolectins. Phytohaemagglutinin has anumber of physiological effects and is used in medicalresearch. It not only induces mitosis but also effectsthe transport of proteins across the cell membrane.PHA can be toxic if used in too high concentrations.

PHA is a naturally occurring product and is subject tobatch variation problems. To minimize these variationsPAA provides a pre-tested, ready to use solution with aconstant and defined concentration of PHA. Everybatch of PHA is tested by an independent cytogeneticlaboratory for its capacity to stimulate the growth ofperipheral blood lymphocytes. The resulting karyo-grams are compared with reference standards. PAAdetermines the optimal concentration by differentquality control tests to offer you a defined ready to usesolution because:

Too much PHA can exhibit a toxic effect

Not enough PHA can result in poor mitosis

Features

> Ready to use solution

> Defined concentration

> Optimized for best lymphocyte stimulation

Order information

J01-003 Colcemid . . . . . . . . . . . . . . . . 10 mlS11-018 Potassium Chloride . . . . . . . . 100 mlS11-019 Giemsa Stain Solution . . . . . . 100 ml For further details and

specifications see page 94.


Transfection

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Transfection Reagent

Nanofectin Kit . . . . . . . . . . . . . . . . . . . . . . . 116

Transfection Antibiotics

G-418 Sulphate . . . . . . . . . . . . . . . . . . . . . . 117Hygromycin B . . . . . . . . . . . . . . . . . . . . . . . 118Blasticidin . . . . . . . . . . . . . . . . . . . . . . . . . . 119Carbenicillin . . . . . . . . . . . . . . . . . . . . . . . . 120Puromycin . . . . . . . . . . . . . . . . . . . . . . . . . . 120


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TransfectionTransfection is the transport of DNA or RNA intoeukaryotic cells. To ensure high efficiency in trans-fection, as well as gentle and stable cell cultureconditions, PAA provides transfection reagents anda variety of selection antibiotics for your preferredtransfection method.


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A method often used by researchers in the field ofmolecular biology is the controlled transport of exter-nal DNA or RNA into eukaryotic cells. This so calledtransfection uses the services of plasmids, transposonsor viruses to incorporate the DNA / RNA into the cellgenome and allows the analysis of the regulation andexpression of genes or the production of recombinantproteins.

Transfection Methods

The transfection of DNA can be either transient orstable. When performing a transient transfection theexpression of transfected genes in cells is usually mea-sured within 16 to 96 hours. However a stable trans-fection integrates the DNA into the genome of the celland therefore allows unlimited analysis of the genefunction. One also can transfect several genes in paral-lel with the co-transfection method.

There are various methods of introducing DNA into acell, for example chemical or physical methods. Thechemical transfection uses calcium phosphate, DEAE-Dextran liposomes, organic polycationic polymers anddendrimers whereas the physical transfection is eitherdone by microinjection or electroporation.

Requirements for Transfection Reagents

Transfection reagents differ considerably in transfec-tion efficiency. Most reagents transfer DNA inefficient-ly as cells only take up particles of a specific size. Inaddition, most reagents cannot sufficiently protect theDNA-complex against endonuclease degradation. Newcarrier molecules with new chemical features allow theDNA-complexes to be taken up more efficiently, thusincreasing the transfection rate. Another issue thatcomes up frequently when choosing among differentgene delivery methods is the cytotoxicity of the variousreagents.

Selection Antibiotics

For the differentiation between transfected and non-transfected cells, researchers use selection-markerswhich are co-transfected with the external DNA. Inmany cases they use resistance genes for antibioticslike the neo-gene which encodes a resistance againstGeneticin. Other selectable markers such asPuromycin, Hygromycin B, Blasticidin or Carbenicillinare also frequently used.

PAA's Product Line

Based on principles of nanotechnology PAA developeda new transfection reagent. Nanofectin is taken up bythe cells in a very efficient way and protects the boundDNA against endonuclease activities. Nanofectin con-sists of two components, a porous nanoparticle and apositively charged polymer which encases the nano-particle. The complex easily passes the core membraneand the external DNA is integrated into the eukaryoticcell DNA. Nanofectin can be used for adherent as wellas non adherent cells and can also be used for stableand transient transfection.

Introduction



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NanofectinTransfection Reagents for Mammalian Cells

Requirements for Transfection Reagents

Transfection reagents differ considerably in trans-fection efficiency. Most reagents transfer DNA ineffi-ciently as cells only take up particles of a specific size.In addition, most reagents cannot sufficiently protectthe DNA-complex against endonuclease degradation.New carrier molecules developed by PAA allow theDNA-complexes to be taken up more efficiently, thusincreasing the transfection rate.

The Development

Nanofectin consists of two components, a positivelycharged polymer which is coiled into a porous nano-particle. The specific particle size and structure ofNanofectin allows DNA to be taken up by cells moreefficiently. By the principle of surface area enlarge-ment, the DNA binding capacity is increased.

Uptake of the DNA-nanoparticle Complex

Modern nanotechnology enables the production ofnanoparticles of uniform size for an optimized uptakeof the DNA-nanoparticle complex.

Nanoparticles protect DNA

After transfection and release of the DNA-nano-particles into the cell cytoplasm, the structure ofNanofectin protects the bound DNA against endo-nucleolytic degradation. This allows for successfultransfection using lower quantities of DNA.

Nanofectin for stable and transient Expression

DNA bound to Nanofectin can pass the nuclear mem-brane. Within the nucleus, stable integration into thechromosome can occur. Transient expression in thecytoplasm is also extended with the use of Nanofectin.

Easy Transfection

Nanofectin is extremely easy to use. The DNA has to bediluted with PAA's Diluent and incubated withNanofectin for 30 minutes before the mixture is addedto the appropriate cells. The expression is measuredafter 24-48 hours. Nanofectin can be used in thepresence of serum so there is no need to rinse cellsbefore incubation with the Nanofectin-DNA complexes.


> Easy to use

> Transfection in the presence or absence of serum

> Effective for stable and transient transfection

> High transfection rates

> Tested on primary, adherent- and suspension cells

> Non-toxic

> Stable for 12 months

> Ready to use kit containing PAA's Diluent

Order information

Q050-005 Nanofectin Kit Nanofectin . . 500 µl Diluent . . . . . 50 ml

Q051-005 Nanofectin Kit Nanofectin . 1000 µl Diluent . . . . . 50 ml



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G-418 Sulphate

Selective Antibiotic

G-418 Sulphate is an aminoglycoside antibiotic isola-ted from Micromonospora rhodorangea. Its structureis similar to gentamicin, neomycin and kanamycin andit interferes with the function of 80S ribosomes in pro-tein synthesis in eukaryotic and prokaryotic cells. G-418 blocks polypeptide synthesis by binding irrever-sibly to 80S ribosomes and inhibiting the elongationstep. G-418 is used as a selective antibiotic in molecu-lar and cell biology. It is usually used for the selectionand maintenance of mammalian, plant or yeast cells.

Resistance to G-418

Resistance is conferred by the neo gene from transpo-sons Tn5 and Tn601 encoding the aminoglycoside 3´-phosphotransferases, APH 3´II and APH 3´I. These pro-teins inactivate G-418 by covalently modifying theiramino or hydroxyl functions therefore inhibiting theantibiotic-ribosome interaction.

Chemical Properties

Formula: C20H40N4O10 x 2H2SO4

Molecular Weight: 692.7g/mol


The working concentration in mammalian cells is bet-ween 100 and 1000 µg/ml. Dividing cells are moresensitive to G-418 than non dividing cells.

Order information

P21-011 G-418 Sulphate . . . 1 gP25-011 G-418 Sulphate . . . 5 gP31-011 G-418 Sulphate . . 10 g

P02-012 G-418 Sulphate . . . 20 mlP11-012 G-418 Sulphate . . 100 ml



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Hygromycin B

Selection Antibiotic

Hygromycin B is a unique aminoglycoside antibioticisolated from Streptomyces hygroscopicus. It can beused as a selective antibiotic in molecular genetics andin cell culture experiments to inhibit the growth ofprokaryotic (bacteria) and eukaryotic microorganisms(yeasts) as well as mammalian cells. Resistance toHygromycin B is used for the identification or selectionof recombinant clones in a variety of cell types.

Resistance to Hygromycin B

Hygromycin B resistance is conferred by the E. coligene hph which is coding for the kinase HPT(Hygromycin phosphotransferase). This kinase inactiva-tes Hygromycin B through phosphorylation. By recom-binant DNA techniques the gene was isolated and avariety of vectors have been constructed containingthe resistance gene fused with eukaryotic promoters tomediate Hygromycin B resistance in various cells.

Hygromycin B inhibits the protein synthesis at thetranslocation step by interfering with the 70S riboso-me causing misreading of the mRNA. It is effective onmost bacteria, fungi and higher eukaryotes.

Sensitivity

The sensitivity of cells for Hygromycin B depends onthe pH of the culture medium. The sensitivity rises withan increasing pH. Thus, the concentration ofHygromycin B required for complete growth inhibitionof given cells can be reduced by increasing the pH ofthe medium. Using media with a lower salt concentra-tion can also result in a decrease of the Hygromycin Bneeded.

Chemical Properties

> Formula: C20H37N3O13 x HCl

> Molecular Weight: 527.0 g/mol


Most cells growing aerobically are killed byHygromycin B in the concentration range of between50 and 800 µg/ml.

Order information

P15-014 Hygromycin B . . . . . . . . . . . . 500 mgP21-014 Hygromycin B . . . . . . . . . . . . . . . 1 gP02-015 Hygromycin B

Solution (50mg/ml) . . . . . . . . . 20 ml



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Blasticidin


Blasticidin is a peptidyl nucleoside antibiotic isolatedfrom broth cultures of Streptomyces griseochromoge-nes. It specifically inhibits the protein synthesis in pro-karyotic and eukaryotic cells by inhibiting the forma-tion of peptide bonds in the ribosomal machinery.

Resistance to Blasticidin

Blasticidin is used to select transfected cells carryingresistance genes. There are three genes which can beused for transformation experiments in mammalianand plant cells.

> The acetyl transferase gene bls

> The deaminase gene bsr from Bacillus cereus

> The deaminase gene BSD from Aspergillus terreus

Blasticidin resistance is conferred by the blasticidindeaminase which converts blasticidin to a non-toxicdeaminohydroxy derivative.

Selection Conditions

Blasticidin is useful in experiments conducted withmammalian cells but it is poorly active on E.coli.

Chemical Properties

> Formula: C17H26N8O5 x HCl



The typical working concentration of Blasticidin isbetween 3 and 50 µg/ml for mammalian cell selection.

Order information

P05-017 Blasticidin . . . . . . . . . . . . . . . . 50 mgP11-017 Blasticidin . . . . . . . . . . . . . . . 100 mg


Order information

P11-019 Puromycin . . . . . . . . . . . . . . 100 mgP15-019 Puromycin . . . . . . . . . . . . . . 500 mg


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Carbenicillin


Carbenicillin (or alpha-carboxybenzylpenicillin) is asemi-synthetic antibiotic used for gene expressionresearch utilizing carbenicillin-resistant plasmids. Theantibiotic is non-toxic to plant cells and is thereforeused for the elimination of Agrobacterium speciesafter transformation. Furthermore carbenicillin is high-ly active against gram negative bacteria. It preventsbacterial cell wall synthesis by binding and inactivatingtranspeptidases which are needed for the crosslinkingof peptidoglycan.

Resistance to Carbenicillin

Carbenicillin is recommended for use instead of ampi-cillin to maintain the selective marker bla which iscoding for a β-lactamase. Ampicillin selection tends tobe lost in cultures as the drug is degraded.Carbenicillin is less sensitive to low pH and so the lossof drug resistance can be avoided. Furthermore carbe-nicillin is found to be less sensitive to the activity ofβ-lactamase then ampicillin. The use of carbenicillininstead of ampicillin helps prevent the overgrowth ofsatellite colonies.


Agrobacterium is used to transform dicot plants (e.g.Nicotiana tabacum) with the Ti-plamid system.Successfully transformed plant cells are selected by theantibiotic kanamycin. Using carbenicillin theAgrobacteria are removed after transformation.

Chemical Properties

> Formula: C17H16N2O6S x 2Na



The working concentration for plant transformationwith Agrobacterium is between 50 and 300 µg/ml (forE. coli: 50 - 100 µg/ml).

Order information

P21-020 Carbenicillin . . . . . . . . . . . . . . . . 1 gP25-020 Carbenicillin . . . . . . . . . . . . . . . . 5 gP31-020 Carbenicillin . . . . . . . . . . . . . . . 10 g

Puromycin


Puromycin is an aminonucleoside antibiotic isolatedfrom Streptomyces alboniger. It specifically inhibits thepeptidyl transfer on ribosomes of prokaryotic andeukaryotic cells. Puromycin inhibits the growth of grampositive bacteria as well as various animal and insectcells and is used for the selection of mammalian cellswhich have been transformed by vectors expressingpuromycin-N-acetyl-transferase.

Resistance to Puromycin

Puromycin resistance to mammalian cells is conferredby the Streptocmyces alboniger gene pac which iscoding for the puromycin N-acetyl-transferase (PAC).The pac gene is located in a region of the pur clusterlinked to other genes determining the puromycin bio-synthetic pathway.


Puromycin is usually used in experiments conductedwith mammalian cells. It can be useful as an alternati-ve to the neomycin system for transfection experi-ments.

In some particular conditions Puromycin can also beused for the selection of E.coli transformants but it ispoorly active on E.coli.

Chemical Properties

> Formula: C22H29N7O5 x 2HCl



The recommended working concentration of Puromycinis 1 to 10 µg/ml.




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Media

Neuronal Base Medium . . . . . . . . . . . . . . . . 125Neuronal Base Medium AD . . . . . . . . . . . . . 126

Supplements

B27 NeuroMix . . . . . . . . . . . . . . . . . . . . . . . 127Neuronal Stem Cell Supplement . . . . . . . . . . 128G5 Supplement . . . . . . . . . . . . . . . . . . . . . . 129N2 Supplement . . . . . . . . . . . . . . . . . . . . . . 130


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Neuronal Cell CultureNerve cells appear to be more demanding in theirchoice of media and growth supplements thanmany other cells. Therefore PAA supplies a range ofspecial neuronal cell culture products.


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Neuronal Cells of the Nervous System

The nervous systems of the vertebrate animals areoften divided into a central nervous system (CNS) andthe peripheral nervous system (PNS). The CNS consistsof the brain and spinal cord. The PNS consists of allother nerves and neurons which are not part of theCNS.

Cell Types and Functions

The nervous system of an animal coordinates the acti-vity of the muscles, monitors the organs, constructsand processes input from the senses and initiatesactions. The nervous system consists basically of twotypes of cells. Neurons are the primary cells of the ner-vous system and responsible for impulse or signaltransmission. Glias are secondary cells involved in nou-rishment and structural support. Many neurons haveinsulating sheaths of myelin around their axons. Thesheaths are formed by glial cells (commonly called neu-roglia or simply glia) which are non-neuronal cells thatprovide support and nutrition, form myelin, and parti-cipate in signal transmission. The main cell types ofglial cells consist of astrocytes which are responsiblefor coating of axons, producing the myelin sheathsand oligodendrocytes which provide neurons withfood.

Research on Cells of the Nervous System

Neurons are the most important cells for signal trans-mission in the nervous system. Little is known aboutthe mechanism of regulation and development. Tostudy the molecular basis of neurons and their suppor-ting glial cells it is necessary to cultivate the cells sepa-rately under defined conditions. Research of cells ofthe nervous system has traditionally focused on kee-ping neurons alive. The focus was the result of thedogma that adult mammalian cells of the nervoussystem were incapable of generating new cells. Withthe discovery of stem cells in the embryonic and adultcentral nervous system neuronal cells can be studiedunder proliferating conditions.

Neuronal Stem Cells

Neuronal stem cells (NSC), with the potential for selfrenewal and to differentiate into neurons and glial cellscan be grown under serum free conditions in the pre-sence of specific growth factors. In vitro grown NSCenables researchers to study basic development andneural re-implantation into human beings.

PAA's Products for Neuronal Cell Culture

For detailed studies of neurons and neuronal stem cellsdefined conditions are necessary. Nutrient media sup-plemented with foetal bovine serum contains factorswhich can inhibit growth of neuronal cells. Thereforespecific serum free media were developed for optimalsurvival and growth of embryonic and adult neurons.For proliferation and differentiation of neuronal stemcells specific cytokines are necessary.

PAA offers a complete product line for serum free cul-tivation not only for embryonic and adult neurons butalso for the proliferation of neuronal stem cells. For dif-ferentiation of neuronal stem cells into neurons andastrocytes PAA provides different supplements. Thenewly developed basal media and cytokines alsosupport the differentiation of murine embryonic stemcells into neural lineages.

Introduction

Product Application Cat. No.

B27 NeuroMix > supplement for adult neurons F01-002(in combination with Neuronal Base Medium AD)

> supplement for embryonic neurons (in combination with Neuronal Base Medium)

> supplement for differentiation of neuronal stem cells into astrocytes and neurons

> can be combined with G5 Supplement, N2 Supplement,DMEM/Ham's F-12 or Neuronal Base Medium

Neuronal Stem > for expansion of neuronal stem cells F01-005

Cell Supplement> avoids differentiation

> can be combined with G5 Supplement, DMEM/Ham's F-12or Neuronal Base Medium

G5 Supplement > supplement for growth of primary embryonic neurons F001-003(in combination with Neuronal Base Medium)

> supplement for expansion of neuronal stem cells (in combination with Neuronal Stem Cell Supplement)

> in combination with B27 NeuroMix for differentiationof neuronal stem cells

N2 Supplement > supplement for primary and serial tumour cell lines of glial origin F005-004(in combination with Neuronal Base Medium or DMEM/Ham's F-12)

> supplement for differentiation of neuronal stem cells into astrocytes and neurons (in combination with B27 Neuro Mix)

Neuronal Base > base medium for embryonic neurons U15-023Medium (in combination with B27 NeuroMix)

> base medium for differentiation and growth of neuronal stem cells

> can be combined with neural specific supplements (B27 NeuroMix for differentiation)

Neuronal Base > base medium for adult neurons U15-024Medium AD (in combination with B27 NeuroMix)


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The right Product for your Application

PAA offers a complete product line for growth of embryonic and adult neurons, glial cells and differentiation ofneuronal stem cells. To facilitate your choice of the optimal culture medium and supplement combination pleaserefer to the table below.



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General

Classical basal media are not optimized for thecultivation of neurons. Some components such asasparagine and aspartic acid can inhibit growth ofneuronal cells. Withdrawal of inhibitory factors led tothe development of Neuronal Base Medium with opti-mal growth properties for embryonic neurons.

PAA's Neuronal Base Medium is formulated to meetthe special requirements of embryonic neurons andneuronal stem cells. In combination with severalNeuroMixes (B27 NeuroMix, Neuronal Stem CellSupplement) this medium allows growth of neuronalcells without the need of astrocyte feeder cells.

Application

Neuronal Base Medium is an optimized medium forembryonic neurons. The specific components reduceovergrowth of glial cells. Only under glia-free condi-tions reliable results can be provided.

Neuronal Stem Cells

For serum free cell culture of neuronal stem cells defi-ned culture conditions are necessary. Neuronal BaseMedium contains an optimized and balanced formu-lation of trace elements, salts, amino acids, fatty acidsand vitamins. In combination with the NeuroMixes G5or N2 embryonic stem cells can be differentiated intoneuronal cells. Furthermore using B27 NeuroMix neu-ronal stem cells can be differentiated into neuronalcells and astrocytes. By use of Neuronal Stem CellSupplement adult neuronal stem cells can proliferatewithout differentiation.

Composition

The basal medium contains vitamins, trace elementsand amino acids which are optimized for growth ofneuronal cell lines.

Features

> Serum free cell culture of neuronal cells

> For reduction of glial cells

> Optimal growth of embryonic neurons

> For proliferation of neuronal stem cells

Order information

U15-023 Neuronal Base Medium . . . . . 500 ml



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reNeuronal Base Medium AD

General

Classical basal media are not optimized for cultivationof neurons. Some components such as asparagine andaspartic acid can inhibit growth of neuronal cells.Withdrawal of inhibitory factors led to the develop-ment of Neuronal Base Medium AD (adult) with optimalgrowth properties for long term cultivation of adultneurons of the Central Nervous System (CNS) andPeripheral Nervous System (PNS).

PAA's Neuronal Base Medium AD is formulated tomeet the specific requirements of adult neurons. Incombination with B27 NeuroMix this medium allowsgrowth of neuronal cells without the need of astrocy-te feeder cells.

Application

Neuronal Base Medium AD is an optimized mediumfor adult neurons. The specific components reduceovergrowth of glial cells. Only under glia-free condi-tions reliable results can be provided.

Composition

Neuronal Base Medium AD is based on the successfulNeuronal Base Medium containing additional salts lea-ding to a higher osmolality. Furthermore it containsvitamins, trace elements and amino acids which areoptimized for growth of adult neurons.

Features

> Serum free cell culture

> Optimal growth of adult neurons

> Long term cultivation of postnatal rat hippocampaland other adult mammalian neurons

> Long term cultivation of different adult neuronsfrom the Central Nervous System (CNS) andPeripheral Nervous System (PNS)

Order information

U15-024 Neuronal Base Medium AD . . 500 ml



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B27 NeuroMix

General

Cells of the central nervous system need specificgrowth factors for long-term viability. Additionallymore vitamins and fatty acids can enhance the long-term cultivation. The recently developed B27 NeuroMixis a multi-purpose serum free supplement for long-term viability and growth of hippocampal and otherneurons of the Central Nervous (CNS) and PeripheralNervous System (PNS). B27 NeuroMix contains alsospecific growth factors for differentiation of neuronalstem cells and differentiation of murine embryonicstem cells into neuronal lineages. It is based on theneuron-specific B27 Supplement developed by Brewerbut with an improved formulation.

Applications

For detailed studies of neurons defined nutrient mediafor growth are necessary. Basal media supplementedwith foetal bovine serum contain factors which caninhibit growth of neuronal cells. Therefore specificserum substitutes were developed for optimal survivaland growth of embryonic and adult neurons to elimi-nate neurotoxic side effects.

When added to Neuronal Base Medium, B27NeuroMix supports the growth of neuronal cellswithout the need of specific feeder cells.

Differentiation of neuronal Stem Cells

For differentiation of neuronal stem cells the supple-ment contains Vitamin A. B27 NeuroMix can also beused together with G5 Supplement to improve growthand differentiation of neuronal stem cells into glial cellsand neurons. For differentiation of murine embryonicstem cells into neuronal lineages B27 NeuroMix andG5 Supplement provide essential growth factors andhormones.

Composition

The supplement is a chemically defined 50x concen-trate and contains vitamins, hormones and othergrowth factors including insulin, transferrin, catalase,superoxide dismutase, antioxidants and fatty acids.

Features

> Multi-purpose serum substitute

> Long-term viability and growth of neurons

> No feeder cells required

> Growth of hippocampal neurons

> Variety of CNS neurons

> Growth factors for differentiation of neuronal stemcells

> Optimized composition for differentiation of murineembryonic stem cells into neuronal lineages

> For differentiation of neuronal stem cells into astro-cytes and neurons

Order information

F01-002 B27 NeuroMix 50x Concentrate . . . . . . . . . . .10 ml



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General

With the discovery of stem cells in the embryonic andadult central nervous system neurobiological researchgained new research tools. Neuronal cells can now bestudied under proliferating conditions. Neuronal StemCells (NSC's), with the potential for self renewal and todifferentiate into neurons and glial cells can be grownin vitro under serum free conditions in the presence ofspecific growth factors. In vitro grown NSC's are idealfor neuronal re-implantation experiments.

Applications

For proliferation of neuronal stem cells under definedconditions a serum free supplement without inducersof neural differentiation is necessary. With the deve-lopment of our Neuronal Stem Cell Supplement defi-ned conditions for optimal proliferation of neurosphe-res and neuronal stem cells are possible.

Composition

The supplement (50x concentrate) contains vitamins,hormones and other growth factors such as insulin,transferrin, catalase, antioxidants, fatty acids and fattyacid precursors. The complex mixture of different anti-oxidants reduces the reactive oxygen damage.Neuronal Stem Cell Supplement derived from B27NeuroMix contains no retinoic acid. Removal ofVitamin A prevents neural differentiation of stem cells.

Application

When added to Neuronal Base Medium orDMEM/Ham´s F-12, the supplement enhances the pro-liferation of neuronal stem cells. The proliferation ratecan be increased by adding G5 Supplement.


> Serum free cultivation

> Complex mixture of growth factors

> No differentiation of neuronal stem cells

> Optimized for proliferation of neuronal stem cells

Order information

F01-005 Neuronal Stem Cell Supplement

50x Concentrate . . . . . . . . . . . .10 ml



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G5 Supplement

General

Foetal Bovine Serum contains factors which can inhibitgrowth of neuronal cells of the nervous system. Forcultivation of glial and primary cell lines of astrocytephenotype a specific serum free supplement has beendeveloped. The G5 Supplement with EGF and FGFbased on Bottenstein´s G5 formulation is also optimizedfor serum free proliferation and differentiation ofneuronal stem cells into neurons and astrocytes.

G5 Supplement is a multi-purpose supplement forserum free cultivation of glial cells and neuronal stemcells.

Applications

Glias are secondary cells involved in nourishment andstructural support of neurons. The main cell types ofglial cells consist of astrocytes which are responsiblefor producing the myelin sheaths and oligo-dendrocytes which provide neurons with food. Fordetailed studies of different types of glial cells adefined serum substitute must be used. With G5Supplement a complete set of optimized growthstimulating factors is available. It is recommended tocombine G5 Supplement with the basal media DMEMor DMEM/Ham's F-12.

Proliferation and Differentiation

Neuronal stem cells can be grown under serum freeconditions with a complex mixture of growth factorsand hormones. G5 Supplement can be used for proli-feration of neuronal stem cells. The proliferation ratecan be increased by addition of Neuronal Stem CellSupplement. For differentiation of neuronal stem cellsG5 Supplement must be combined with B27 NeuroMixa serum free supplement containing vitamin A which isnecessary for neurogenesis. Depending on the types ofcells being cultivated G5 Supplement can be substitutedwith N2 Supplement.

Composition

G5 Supplement contains insulin, human transferrin,selenite, hydrocortisone, EGF and FGF.



> Optimized hormone composition

> For cultivation of glial cells


> Important component for differentiation ofneuronal stem cells

> Cultivation of glial and primary cell lines ofastrocyte phenotype

Order information

F001-003 G5 Supplement100x Concentrate . . . . . . . . . . . 1 ml



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reN2 Supplement

General

Growth of neuronal cells of the nervous system can beinhibited by some components of Foetal Bovine Serum(FBS). In order to avoid inhibitory effects of FBS neuro-nal cells must be cultivated under serum free condi-tions. In the past different serum free supplementshave been developed. The N2 Supplement belongs toa small and successful group of multi-purpose serumfree substitutes for growth of sensitive cells of the ner-vous system. It is qualified for cultivation of primaryembryonic neurons and optimized for growth of neu-ronal stem cells.

Applications

> Cultivation of primary embryonic neurons

It is recommended to combine N2 Supplement withthe Neuronal Base Medium.

Proliferation and Differentiation

In vitro grown neuronal stem cells provide the oppor-tunity to study basic development together with thepossibility of neuronal re-implantation experiments.Serum free cultivation of neuronal stem cells is requi-red for detailed studies. With N2 Supplement, one ofthe most successful multi-purpose serum free supple-ments, neuronal stem cells can be grown under defi-ned conditions. N2 Supplement can be used for proli-feration of neuronal stem cells. The proliferation ratecan be increased by addition of Neuronal Stem CellSupplement.

For differentiation of neuronal stem cells N2Supplement must be combined with B27 NeuroMix, aserum free supplement with vitamin A which is neces-sary for neurogenesis. Depending on the cell types N2Supplement can be substituted by G5 Supplement. It isrecommended to combine N2 Supplement withDMEM or DMEM/Ham's F-12.

Composition

N2 Supplement contains recombinant insulin, humantransferrin, sodium selenite, putrescine and proges-terone.

Features

> No inhibitory effects


> Optimized hormone and growth factor composition

> Growth of primary embryonic neurons

> Selected growth factors for proliferation ofneuronal stem cells

Order information

F005-004 N2 Supplement100x Concentrate . . . . . . . . . . . 5 ml


Stem Cell Culture

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Media

CollagenStem Kit . . . . . . . . . . . . . . . . . . . . . 135MethoStem Medium . . . . . . . . . . . . . . . . . . 135MesenchymStem Medium . . . . . . . . . . . . . . 136Neuronal Base Medium . . . . . . . . . . . . . . . . 136

Sera

FBS ES cell tested . . . . . . . . . . . . . . . . . . . . . 137FBS Pharma Grade . . . . . . . . . . . . . . . . . . . . 138Human Serum, off the clot . . . . . . . . . . . . . . 139Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . 140

Supplements

HematoStem . . . . . . . . . . . . . . . . . . . . . . . . 140Neuronal Stem Cell Supplement . . . . . . . . . . 141B27 NeuroMix . . . . . . . . . . . . . . . . . . . . . . . 141G5 Supplement . . . . . . . . . . . . . . . . . . . . . . 142N2 Supplement . . . . . . . . . . . . . . . . . . . . . . 142


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Stem Cell CultureResearch on stem cells is a very important field ofmedical studies. These fastidious cells require special products based on pretested high qualityraw materials.


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General

Stem cells are unspecialized cells that can self-renewindefinitely and differentiate into more mature cellswith specialized functions. In humans, stem cells havebeen identified in the inner cell mass of the earlyembryo (embryonic stem cells), in some tissues of foe-tuses, the umbilical cord and in several adult organsand tissues (adult stem cells).

Potential of Stem Cells to cure many Diseases

Research on adult and embryonic stem cells has thepotential to produce treatments for several medicalconditions that, at present, lack therapies. Presentresearch would seem to indicate that many conditionscould be treated by transplantations of differentiatedstem cells.

Embryonic Stem Cells

Embryonic stem cells (ESC) are isolated from theembryos early blastocyst stage. They are able to developinto all other cell types that occur in the livingorganism. The establishment of permanent ES cell lineshas been facilitated by co-culture with feeder cells andqualified FBS (human ES) or without feeder cells in thepresence of LIF (leukaemia inhibitory factor) and FBS(murine ES). LIF prevents spontaneous differentiationof ES cells and maintains their developmental potential.The differentiation into nerve-, muscle-, blood-, fat-,and connective tissue cells can be controlled by specificmedia and the use of cell type specific growth factors.

Adult Stem Cells

Adult stem cells are undifferentiated cells that can selfrenew and differentiate into specific cells of the tissuesor organs. The primary roles of adult stem cells is tomaintain and repair the tissue in which they are found.The in vitro differentiation into mature cells can becontrolled by supplementation of growth media withspecific growth factors and hormones. The potential ofin vitro differentiation is in the regenerative medicinewithout tissue rejection.

Hematopoietic Stem Cells

Hematopoietic stem cells, the blood-forming cells inthe bone marrow, can differentiate into three majortypes of brain cells (neurons, oligodendrocytes andastrocytes), skeletal muscle cells, cardiac muscle cellsand liver cells. For in vitro growth and differentiationspecific basal media supplemented with a complex setof hematopoietic growth factors and pre-tested FBSare needed.

Mesenchymal Stem Cells

Bone marrow mesenchymal stem cells can differentia-te into different cell types: bone cells (osteocytes), car-tilage cells (chondrocytes), fat cells (adipocytes) andother kinds of connective tissue cells. For in vitro culti-vation of mesenchymal stem cells specific basal mediaare needed supplemented with a high concentrationof qualified FBS (10-20%). Mesenchymal stem cellscan be differentiated in vitro by induction with specificfactors.

Neuronal Stem Cells

Neuronal stem cells (NSC), with the potential for self-renewal and to differentiate into neurons and glial cellscan be grown in vitro under serum free conditions inthe presence of specific growth factors. In vitro grownNSC are used to study basic cell development andneuronal re-transplantation into human beings.

Semi-solid Media in Stem Cell Culture

Methylcellulose media are used for simplified colonyformation, separation and identification of hemato-poietic and embryonic stem cells. For further analysisdried collagen-based media are ideal for fixation withcytochemical or immuno-cytochemical staining reagents.

FBS ES cell tested

For cultivation of embryonic and adult stem cells ES pre-tested FBS is one of the most important sources ofgrowth factors. By addition of various cytokines the diffe-rentiation can be induced.

Introduction


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Mesenchymal Stem Cells

> Growth and maintenance of mesenchymal stem cells

Hematopoietic Stem Cells

> Growth and in vitro differentiation of hemato-poietic stem cells and their progenitors

> Cytokine-directed formation of hematopoieticprogenitors in semi-solid media

> Long-term growth of hematopoietic progenitors(myeloid cells)

> Formation of embryoid bodies as precursors forhematopoietic cells

> Growth and maintenance of undifferentiated EScells for in vitro differentiation to hematopoieticcells and progenitors

Product Guide

Embryonic Stem Cells

> Growth and maintenance of undifferentiated EScells for in vitro differentiation to hematopoietic orneuronal cells

> Formation of embryoid bodies

> Growth and maintenance of human ES cells forpossible re-implantation experiments

> Cytokine-directed formation of hematopoieticcolonies in semi-solid media

Neuronal Stem Cells

> Growth, differentiation and maintenance ofneuronal stem cells

> Cytokine-directed formation of cells of neuronlineages

Information Embryonic Neuronal Mesenchymal HematopoieticProducts reference Stem Cells Stem Cells Stem Cells Stem Cells

FBS ES cell tested Page 137 • • •Horse Serum Page 140 •Human Serum,

Type AB, off the clotPage 139 •

MethoStem Medium • • •CollagenStem Kit Page 135 •MesenchymStem Medium Page 136 •Neuronal Base Medium Page 136 • •IMDM Page 20 • •MEM Alpha Mod. Page 24 •DMEM, low Glucose Page 14 •DMEM, high Glucose,

with sodium pyruvatePage 15 •

DMEM/Ham's F-12 Page 17 • •HematoStem Page 140 • •B27 NeuroMix Page 141 • •G5 Supplement Page 142 • •N2 Supplement Page 142 •Neuronal Stem

Cell Supplement Page 141 •

PAA offers a large range of different basal media, growth supplements and reagents for the cultivation of embryonic,hematopoietic, neuronal and mesenchymal stem cells. The extensive applications in the research area of stem cellcultivation include amongst others:


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CollagenStem Kit

General

Various semi-solid culture systems have been used fordetection and differentiation of hematopoietic stemcells. Both methylcellulose-based media and collagen-based media are available.

Methylcellulose-based cultures cannot be fixed forsubsequent cytochemical or immuno-cytochemicalstaining. Therefore collagen gels are used for thisapplication. Collagen-based media are an attractivealternative to methylcellulose-based semi-solid cultures.Collagen, a gelling agent, increases the viscosity of themedium allowing optimal colony formation ofdifferent progenitor cells for simplified separation andidentification. Collagen can be fixed for cytochemicalor immuno-cytochemical staining and the resultingslides can be stored long-term.

Composition

The CollagenStem Medium consists of a modifiedIMDM medium containing BSA, 2-mercaptoethanoland L-glutamine. This medium has to be supplemen-ted with the CollagenStem Solution

Furthermore FBS (10 –30%) and cytokines can beadded.


> Detection and quantification of hematopoieticprogenitors

> Formation and isolation of distinct colonies

> Cytochemical or immuno-cytochemical staining

> Serum free basal medium

> Good optical clarity

> Easy visualisation

Order information

U115-033 CollagenStem KitCollagenStem Medium . . . . . . 100 ml CollagenStem Solution . . . . . . . 50 ml

MethoStem Medium

General

For cultivation of hematopoietic stem cells a variety ofdifferent semi-solid culture systems have beendeveloped. Methylcellulose is widely used as a gellingagent which increases the viscosity of the mediumwithout converting it to a solid. Methylcellulose-basedmedia are used in addition to optimal levels of growthfactors for the cultivation of hematopoietic andembryonic stem cells and their progenitors. In additionmethylcellulose increases the viscosity of the mediumto allow colony formation of different progenitor cellsfor simplified separation and identification. For furtherdifferentiation of the stem cells cytokines must beadded.

Methylcellulose-based cultures cannot be fixed forcytochemical or immunocytochemical staining.Therefore collagen-based media are recommended.

Composition

MethoStem Medium consists of a modified IMDMmedium supplemented with methylcellulose, BSA, 2-mercaptoethanol and L-glutamine. FBS (10 –30%)and cytokines have to be added before usage.


> Growth and differentiation of hematopoietic stem cells

> Differentiation and characterization of stem cell progenitors

> For further cultivation and simplified separation of single cells by colony selection

> Growth of embryonic stem cells

For further details and specifications see page 35. For further details and specifications see page 36.

Order information

U11-827 MethoStem Medium . . . . . . . 100 ml

General

PAA's Neuronal Base Medium is formulated to meetthe special requirements of adult and postnatal neuronsof the central nervous system (CNS) and peripheralnervous system (PNS). In combination with differentNeuroMixes (B27 NeuroMix, Neuronal Stem CellSupplement) the supplemented medium allowsgrowth and differentiation of neuronal stem cellswithout the need of an astrocyte feeder layer.

Applications

For serum free cell culture of adult neuronal stem cellsdefined culture conditions are necessary. NeuronalBase Medium contains an optimized and balancedformulation of trace elements, salts, amino acids, fattyacids and vitamins. In combination with differentNeuroMixes, G5 or N2 Supplement embryonic stemcells can be differentiated into neuronal cells.Furthermore with B27 NeuroMix neuronal stem cellscan be differentiated into neuronal cells and astro-cytes. By using Neuronal Stem Cell Supplement adultneuronal stem cells can proliferate without differen-tiation.

Features

> Optimized basal medium

> For proliferation or differentiation of neuronal stem cells

> For differentiation of embryonic stem cells into neuronal cells


MesenchymStem Medium

General

Mesenchymal stem cells, part of the hematopoieticstem cell network, have become a more and moreimportant part of research into human therapeutics.Mesenchymal stem cells, derived from bone marrow,are capable of self-renewal and differentiation intobone-, cartilage-, muscle- and fat cells. Differentiationinto distinct cell types can be achieved by directedstimulation using cytokines. In vivo tissue repair usingdifferentiated cells is possible.

Applications

Mesenchymal stem cells can be prepared after separa-tion of mononuclear cells by density gradient centrifu-gation. Proliferation of mesenchymal stem cells can beincreased by using media enriched with specificgrowth factors from FBS. MesenchymStem Mediumdeveloped by PAA provides the optimal concentrationof growth factors needed for expansion of mesenchy-mal stem cells and their progenitors.

For further differentiation into adipocytes, chondro-cytes or osteocytes different additives, such as dexa-methasone, isobutyl-methylxanthine, ascorbic acidphosphate and indomethacin must be added toMesenchymStem Medium.

Composition

MesenchymStem Medium is a modified DMEMmedium with specific trace elements, FBS and L-gluta-mine. For differentiation of mesenchymal cells intobone-, cartilage-, muscle- and fat cells cytokines mustbe added.

Features


> Optimized formulation for expansion of mesenchy-mal cells

> Simplified characterization and quantification ofmesenchymal progenitors

> Optimal medium for further differentiation experi-ments

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Order information

U15-828 MesenchymStem Medium . . . 500 ml


Order information

U15-023 Neuronal Base Medium . . . . . 500 ml




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Foetal Bovine SerumES cell tested

General

Foetal Bovine Serum (FBS) contains the highest con-centration of growth factors compared with othersera. Therefore FBS is the optimal supplement innutrient media for in vitro growth of different primary-,tumour- and stem cells.

Applications

For proliferation and differentiation of embryonic andadult stem cells high concentrations of growth factors,hormones and other growth stimulating additives areneeded. In embryonic stem cells FBS can promote theformation of aggregates known as embryoid bodies.The embryoid bodies are important intermediates forfurther differentiation into neuronal or hematopoieticprogenitors.

Only selected FBS can provide the different growth fac-tors necessary. Normally FBS quality can vary frombatch to batch. To provide growth factors in constantconcentrations FBS must be tested with embryonicstem cells for efficiency. PAA's FBS ES cell tested givesthe customer the guarantee for reliable and reproduci-ble results in cultivation of stem cells.

Test Parameters

Foetal Bovine Serum, tested on murine embryonicstem cells, is specially tested for the ability to sustainundifferentiated cellular morphology of embryonicstem cells in the presence of LIF (leukaemia inhibitoryfactor). The screening includes plating efficiency, colo-ny morphology and toxicity tests.

Extensive virus tests avoid the risk of virus contamina-tions. Our FBS is tested for PI-3, BHD-I, BVD-AB, andBVD-V and for mycoplasma.

Features

> Special pre-selected serum batches

> Low endotoxin content


> For embryonic stem cells

> For formation of embryoid bodies

> For proliferation and differentiation of stem cells

> For mesenchymal stem cells

Order information

A11-208 Foetal Bovine Serum ES cell tested . . . . . . . . . . . . . 100 ml

A15-208 Foetal Bovine Serum ES cell tested . . . . . . . . . . . . . 500 ml



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Foetal Bovine SerumPharma Grade

General

Foetal Bovine Serum (FBS) contains the highest con-centration of growth factors compared to other sera.For in vitro cultivation of different stem cells FBS is theoptimal supplement in nutrient media. In order to minimize the contamination risk from ani-mal derived products, PAA's FBS Pharma Grade ismanufactured and tested according to current relevantdirectives, guidelines and regulations of Ph. Eur. (EP),EMEA and FDA.

The new Standard

For proliferation and differentiation of embryonic andadult stem cells high concentrations of growth factorsand hormones are needed. Only high quality FBSwhich is tested with different embryonic and adultstem cells reduces the effect of batch to batch varia-tions.Therefore FBS Pharma Grade is screened extensivelyfor ten different viruses (Parainfluenza Type 3 (PI-3),Bovine Virus Diarrhoea Virus (BVD-V), Bovine HerpesVirus Type 1 (BHV-1), Blue Tongue Virus (BTV), BovineAdenovirus (BAV), Bovine Reovirus (BRV), BovineParvovirus (BPV), Bovine Respiratory Syncytial Virus(BRSV), Rabies Virus, BVD-1 AB, BVD-2 AB, Rabies AB,BVD AB Inhibitory, BVD AB Interferences).

In order to reduce potential virus contamination FBSPharma Grade can be treated by an effective singlebox gamma irradiation (optional) with 35 kGy.

Product safety will be increased by only using FBS sour-ced from BSE free countries such as Australia.

FBS Pharma Grade for advanced TherapyApplications

FBS Pharma Grade fulfils the requirements of the bio-pharmaceutical industry and demands of the authori-ties in this field. Therefore PAA's high quality FBSPharma Grade tested at the highest safety level is idealfor stem cell cultivation, gene therapy, somatic cell the-rapy, tissue engineering and re-implantation experi-ments.

Features

> Tested according to current biopharmaceutical guidelines for bovine derived products

> No BSE risk> Virus and mycoplasma inactivation by

gamma irradiation> Manufactured according to cGMP

For each batch complete documentation can be provi-ded upon request.

Order information

A15-511 FBS Pharma GradeAUS origin . . . . . . . . . . . . . . .500 ml

A15-512 FBS Pharma Grade, Gamma IrradiatedAUS origin . . . . . . . . . . . . . . .500 ml

A15-211 FBS Pharma GradeUSA origin . . . . . . . . . . . . . . .500 ml

A15-212 FBS Pharma Grade, Gamma IrradiatedUSA origin . . . . . . . . . . . . . . .500 ml



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Human Serum, off the clotType AB

General

Human serum contains high concentrations of growthfactors. Therefore human serum is an optimal supple-ment in nutrient media for in vitro growth of differentprimary-, tumour- and stem cells.

Human Serum – the ideal Growth Supplement

Human embryonic stem cells (hESC) can potentiallydifferentiate into every body cell type, making themideal candidates for cell- and tissue-replacementtherapies. Human ES cells are normally cultured withanimal derived sera in combination with an animalfeeder layer. Recently it was found that hES cellsexpress an immunogenic non-human sialic acid againstwhich many humans have circulating antibodies whencultured with animal derived growth supplements.Therefore hES cells grown in animal derived compo-nents cannot be re-implanted into patients becauseallergic reactions will be induced and the hES cells willbe rejected.

Human Serum with no allergenic Potential

In order to avoid immunogenic reactions human serummust be used in combination with human feeder lay-ers. Only hES cells grown in non animal derived com-ponents can be re-implanted into patients.

PAA's Human Serum with highest Safety

The collection of raw serum is undertaken in FDA con-trolled plasmapheresis centers. The collected rawserum is taken strictly from donors that have beenunder medical control for a minimum of six months.Each single donor unit taken is subjected to extensivetests such as absence of HIV1 and 2, HIV-1Ag, anti-HCV and HbsAg.

Features

> Special serum batches


> Tested for human viruses and antibodies

> Tested for microorganisms

> No allergenic potential

> No formation of sialic acid residues

> For re-implantation experiments

> For growth and maintenance of human embryonicstem cells

Optional: For highest virus safety human serum can begamma sterilized.

Order information

C11-021 Human Serum, off the clotType AB . . . . . . . . . . . . . . . . 100 ml

C15-021 Human Serum, off the clotType AB . . . . . . . . . . . . . . . . . 500 ml


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Horse Serum

General

Horse serum contains specific growth factors whichdiffer from other animal sera. For some hematopoieticcells like myeloid cells only horse serum provides thegrowth stimulating factors which are needed for theirexpansion. Therefore horse serum is the ideal supple-ment in nutrient media for in vitro growth of specifichematopoietic progenitors.

Applications

For long-term cultivation of myeloid cells the essentialgrowth factors are provided by horse serum in combi-nation with FBS ES cell tested.

Quality Control

The raw serum is collected in approved slaughterhou-ses. The collected raw serum is subjected to extensivetests such as equine infectious anaemia.

Features

> Special serum batches



> For growth of myeloid cells

Order information

B11-021 Horse Serum . . . . . . . . . . . . . 100 ml

B15-021 Horse Serum . . . . . . . . . . . . . 500 ml

HematoStem

General

Hematopoietic Stem Cells (HSC) are adult stem cellswhich can be isolated from blood, bone marrow orfrom umbilical cord blood. They are able to replenishthemselves continually and differentiate into a varietyof specialized cells. They are responsible for theconstant renewal of erythrocytes, platelets, lympho-cytes and myeloid cells. Hematopoietic stem cells havebeen studied for many years and were the first stemcells to be used in therapies. HematoStem is anexcellent source of colony stimulating activity forhematopoietic stem and progenitor cells.

Composition

HematoStem Supplement consists of IMDM containing5 % FBS and kanamycin sulphate. Furthermore specificgrowth factors secreted from a tumour cell line areprovided for proliferation and differentiation: granulo-cyte macrophage colony-stimulating factor (GM-CSF),granulocyte colony-stimulating factor (G-CSF), macro-phage colony-stimulating factor (M-CSF), interleukin-1(IL-1), interleukin-6 (IL-6), plasminogen activator andprostaglandin E.

Applications

HematoStem is a potent source of colony stimulatingfactors needed for the cultivation of hematopoieticstem cells and for differentiation of embryonic stemcells into hematopoietic cells and progenitors. The pro-liferation rate can be increased by addition of FBS andother growth factors like Stem Cell Factor (SCF) anderythropoietin.

Features

> Ready to use supplement

> Increased productivity

> No preparation of feeder cell layer required

> For differentiation of embryoid stem cells intohematopoietic cells

> For bone marrow cells

> For proliferation and differentiation of hematopoietic stem cells

> For hematopoietic progenitors (colony forming cells)

> For CFU-E, BFU-E, CFU-GM and CFU-GEMM

Order information

U05-019 HematoStem . . . . . . . . . . . . . . 50 ml



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Neuronal Stem Cell Supplement

General

Neuronal Stem Cell Supplement is a serum free supple-ment for growth and proliferation of neurospheresand neuronal stem cells.

Components

The supplement (50x concentrate) contains vitamins,hormones and other growth factors such as insulin,transferrin, catalase, antioxidants, fatty acids and fattyacid precursors. The complex mixture of different anti-oxidants reduces the reactive oxygen damage.Neuronal Stem Cell Supplement derived from B27NeuroMix, does not contain retinoic acid. Removal ofvitamin A prevents neuronal differentiation of stemcells.

Application

When added to Neuronal Base Medium or DMEM/Ham´sF-12, the supplement enhances the proliferation ofneuronal stem cells. For optimal results PAA recom-mends Neuronal Base Medium (Cat. No. U15-023).


> Serum free cultivation

> Complex mixture of growth factors

> No differentiation of neuronal stem cells

> Optimized for proliferation of neuronal stem cells

Order information

F01-005 Neuronal Stem Cell Supplement 50x Concentrate . . . . . . . . . . . 10 ml

B27 NeuroMix

General

B27 NeuroMix is a serum free supplement for differen-tiation of stem cells of neuronal origin and differentia-tion of murine embryonic stem cells into neuronallineages.

Applications

For differentiation of neuronal stem cells the supple-ment contains vitamin A. B27 NeuroMix can also beused together with G5 Supplement to improve growthand differentiation of neuronal stem cells into astrocy-tes and neurons.

For differentiation of murine embryonic stem cells intoneuronal lineages B27 NeuroMix and G5 Supplementprovide essential growth factors and hormones.

When added to Neuronal Base Medium, B27NeuroMix supports the growth of neuronal cells andneuronal stem cells without the need of specific feedercells.

Composition

The supplement is a chemically defined 50x concen-trate and contains vitamins, hormones and othergrowth promoting factors including insulin, transfer-rin, catalase, superoxide dismutase, antioxidants andfatty acids.

Features

> No feeder cells required

> Growth factors for differentiation of neuronal stemcells

> Optimized composition for differentiation of murineembryonic stem cells into neuronal lineages

> For differentiation of neuronal stem cells into astro-cytes and neurons

Order information

F01-002 B27 NeuroMix50x Concentrate . . . . . . . . . . . 10 ml


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G5 Supplement

General

G5 Supplement is a serum free chemically definedsupplement for proliferation and differentiation ofneuronal stem cells into neurons and astrocytes.

Applications

Neuronal stem cells can be grown under serum freeconditions with a complex mixture of growth factorsand hormones. G5 Supplement can be used for proli-feration of neuronal stem cells. The proliferation ratecan be increased by addition of Neuronal Stem CellSupplement. For differentiation of neuronal stem cellsG5 Supplement must be combined with B27NeuroMix.

The supplement can be used with the following basemedia:

Neuronal Base Medium, DMEM or DMEM/Ham's F-12.For optimal results PAA recommends the use ofNeuronal Base Medium (Cat. No. U15-023).

Composition

G5 Supplement contains insulin, human transferrin,selenite, hydrocortisone, EGF and FGF.




> For differentiation of neuronal stem cells in neurons or astrocytes

Order information

F001-003 G5 Supplement100x Concentrate . . . . . . . . . . . 1 ml

N2 Supplement

General

N2 Supplement is a serum free, chemically definedsupplement based on Bottenstein's N2 formulation.This supplement is optimized for growth of neuronalstem cells. In vitro grown neuronal stem cells are usedto study basic development and neuronal re-trans-plantation.

Applications

Neuronal stem cells can be grown serum free with amixture of growth factors and hormones. N2Supplement can be used for proliferation of neuronalstem cells. The proliferation rate can be increased byaddition of Neuro Stem Cell Supplement. For differen-tiation of neuronal stem cells N2 supplement must becombined with B27 NeuroMix.

The supplement can be used with the following basemedia:

Neuronal Base Medium, DMEM or DMEM/Ham's F-12.For optimal results PAA recommends the use ofNeuronal Base Medium (Cat. No. U15-023).

Composition

N2 Supplement contains recombinant insulin, humantransferrin, sodium selenite, putrescine and proges-terone in PBS.

Features

> Selected growth factors for proliferation of neuronal stem cells


Order information

F005-004 N2 Supplement100x Concentrate . . . . . . . . . . . 5 ml



Cell Preservation

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Preservation Solutions

CryoMaxx S and SF . . . . . . . . . . . . . . . . . . . 146CryoMaxx I and II . . . . . . . . . . . . . . . . . . . . 147CoolStar . . . . . . . . . . . . . . . . . . . . . . . . . . . 148


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Cell PreservationFor hypothermic maintenance and cyropreservationall PAA cell preservation products provide optimalfreezing and cooling conditions with maximumviability and recovery after thawing.


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Cell Preservation

There are two common methods of cell preservation atlow temperature: hypothermic preservation at tempe-ratures above freezing and cryogenic preservation attemperatures below freezing. Hypothermic preser-vation allows short term storage of tissues and organsprior to further investigation. Cryogenic preservation isused for the establishment of cell banks to ensurereproducible results in research by stabilizing livingcells. Using cryopreservation media the cells are pro-tected from damage which can occur during freezingand warming processes. The most commonly used cry-oprotective agents serve several functions during thefreezing process, e.g. freezing point depression, pro-tection of solution effects and extra- and intracellularice crystal formation, but are often toxic in high con-centration.

Therefore cryopreservation media must fulfil the follo-wing requirements:

> Increased long-term storage

> Suppression of ice crystal formation

> Cryoprotective additives with improved formulations

> Highest viability after thawing

Cell Banking

Cryopreservation of cells plays an important role inestablishing cell stocks. Cryopreserved cells assure anadequate supply in case of microbial contamination ofcurrently used cells. By the implementation of a MasterCell Bank (MCB) and Working Cell Bank (WCB) allpotential risk factors may be minimized. Establishing aMCB and a WCB are the first steps in the manufactu-ring process for biopharmaceuticals and can be prepa-red under serum free conditions with defined and ani-mal derived component free cryopreservation media.

In the case of using cryopreservation media withserum, we strongly recommend the usage of foetalbovine serum from Australia for highest virus safety.

Cold storage of Tissues and Organs

For short term preservation tissues and organs can bestored under hypothermic conditions in the presenceof specific media components which prevent the celldecay by reduction of free oxygen radicals and inhibi-tion of different proteases and nuclease. The cold sto-rage of tissues and organs with optimized hypothermicpreservation media is a prerequisite for successful ani-mal organ transplantation.

Several commercial hypothermic preservation mediaare available such as the UW-, Celsior- and HTK- solu-tion. They are based on saccharides and derivates,salts, energy sources, reducing agents and other stabi-lizers.

PAA's new Product Line for Cell Preservation

PAA has developed a family of new cyropreservationsolutions that include new crystal suppressing agents.

Based on successfully developed hypothermic solu-tions for cold storage of organs PAA offers withCryoMaxx I and CryoMaxx II serum free cryopreserva-tion media with different concentrations of DMSO forhighest cell viability after thawing. FurthermoreCryoMaxx SF, a serum free medium based on methyl-cellulose is available.

For classical cryopreservation of cells the serum contai-ning medium CryoMaxx S with highest viability can besupplied.

Tissues and organs can be effectively preserved undercold storage with PAA's new preservation mediumCoolStar based on the well-known UW-, Celsior- andHTK- solutions. Our new formulation reduces oxidativedamage and degradation by proteases and nucleasesmore efficiently. Tissues and organs are successfullyprepared for transplantation experiments.

Introduction


CryoMaxx S and SF for Classical Cryopreservation

General

Cryopreservation is a process used to assure an ade-quate stock of cells and serves as back up in case ofany contaminations of the working cell lines. With astock of frozen cells constant quality can be guaran-teed without change of cellular and genetic features.Only under cyropreserved conditions the cells canmaintain homogenous populations because the cellu-lar metabolisms are reduced to a minimum withoutany genetic drifts.

Classical Cryopreservation Media

Cryopreservation is a procedure to stabilize cells at cry-ogenic temperatures for long term storage in liquidnitrogen. During the freezing process the usage of cry-oprotective additives protects fragile membranes andorganelles of the cells by minimizing the damage asso-ciated with ice crystal formation.

Classical cryopreservation media contain DMSO withstabilizers such as FBS or methylcellulose which increa-se the viability after thawing.

CryoMaxx S and SF

Serum containing cryopreservation media are mostoften used in academic research for cell preservation.CryoMaxx S is a classical serum containing mediumwith 10% DMSO.

For applications that require serum free conditionsfreezing media with other cryoprotectants are needed.CyroMaxx SF is a serum free cryopreservation mediumthat contains 10% DMSO and methylcellulose for cellstabilization.

Composition

CryoMaxx S contains DMEM/Ham's F12 with 20% FBSand 10% DMSO.

CryoMaxx SF is based on DMEM/Ham's F12 containingmethylcellulose and 10% DMSO.

Features


> Contains cryoprotective agents to minimize dehydration effects

> Serum free and serum containing cryopreservation media

> Simple freezing protocol

> High viability of cells after thawing

Order information

J05-013 CryoMaxx S . . . . . . . . . . . . . . . 50 ml

J05-010 CryoMaxx SF . . . . . . . . . . . . . . 50 ml


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CryoMaxx I and II for serum free Cryopreservation

General

Most cell lines are cryopreserved with a serum concen-tration of up to 90% bearing the risk of microbialcontamination. Furthermore storage under serum freeconditions plays more and more an important role notonly in the biopharmaceutical industry but also foracademic research. Therefore PAA developed serumfree cyropreservation media.

Serum free Cryopreservation

Cells can be stored in liquid nitrogen if cryoprotectiveadditives are used. The most effective agent for pre-venting ice crystal formation is DMSO.

In contrast to classical cryopreservation mediaCryoMaxx I and II have a new formulation containingdifferent buffer systems, disaccharides and the colloiddextran which suppress the ice formation during thefreezing procedure more efficiently. In combinationwith different DMSO concentrations the cell viabilitywill be increased significantly.

CryoMaxx I and II

CryoMaxx I and II are defined, serum free cryopreser-vation media and therefore qualified for preparation ofcell banks and use in the biopharmaceutical industry.They contain DMSO as the active substance and diffe-rent new cell stabilizers for best viability after thawing.Due to special components both media can be usedfor cell lines adversely affected by prolonged contactwith DMSO.

Composition

CryoMaxx I (with 5% DMSO) and CryoMaxx II (with10% DMSO) are based on an organ preservationmedium with different saccharides, reducing agentsand colloids for cell stabilization.

Features


> Serum free cryopreservation media

> Contains cryoprotective agents to minimize dehydration effects

> Tested on CHO-, MDCK-, BHK-, HEK 293-, MRC5-, Vero- and HeLa cells

> Improved viability of cells after thawing

> Simple freezing protocol

Order information

J05-011 CryoMaxx I (5% DMSO) . . . . . . 50 ml

J05-012 CryoMaxx II (10% DMSO) . . . . . 50 ml Cel

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CoolStar The hypothermic storage Medium

General

Hypothermic preservation is a procedure to stabilizetissues and organs at +2°C to +8°C. The goal of coldstorage is to induce hypothermia in order to suppressthe rate of cell deterioration. An optimal preservationsolution contains components to reduce cell-swelling(colloids), buffer to maintain pH balance, antioxidantsto scavenge free oxygen radicals and ATP precursors toprovide energy upon reperfusion. The cold storage oftissues and organs with optimized hypothermic preser-vation media is a prerequisite for successful organtransplantation.

For best results, rinsing of biological material prior tohypothermic storage is recommended to removeharmful agents released in the process of extracting.

Applications

For successful tissue and organ transplantation theindividual cells must be preserved with the highest via-bility to maintain the functionality of the whole organ.In contrast to single growing cells only the outermostcells organized in tissues and organs are provided withessential nutrient components, whereas the innermostcells are supplied only by diffusion.

In order to reduce the damaging effects of proteases,nucleases and free radicals CoolStar contains specificinhibitors and components for basic maintenances ofthe cellular metabolism. Only these specific compo-nents allow a high diffusion rate into innermost celllayers assuring optimal supply and preservation.

CoolStar

CoolStar has been formulated to decrease the freeradical accumulation in tissues and organs. Free radicalaccumulation leads to cell death in the case of hypo-thermic preservation. CoolStar contains inhibitors ofproteases and nucleases, and is a further developmentof the well-known UW-, Celsior- and HTK- solutions.CoolStar has all the advantages of the preservationmedium "ViaSpan” combined with further formu-lations to increase the viability prerequisite for success-ful organ transplantation experiments.

CoolStar Rinse, the optimised rinsing solution

After the extracting process it is essential to removepotentially harmful substances that could increase thedeterioration process of the tissue. Therefore CoolStarshould always be used in combination with CoolStarRinse to permit ideal conditions for long term organstorage. It has been formulated as a flush solution torinse culture media and native fluids from mammaliancells, tissues and organs.

CoolStar Kit

For optimal results CoolStar should be used in combi-nation with CoolStar Rinse. Both products are availablein our cost saving CoolStar Kit.

Composition

CoolStar is a hypothermic medium consisting ofdisaccharides (sucrose, mannitol, and glucose), lacto-bionate, dextran, buffers and other reagents whichreduce free radicals. CoolStar Rinse is a solution forrinsing of organs and tissues consisting of differentdisaccharides and buffers.

Features


> Easy to use

> Reduced viscosity

> Gentle cold storage

> Improved viability of cells after 2 - 3 days storage at +2°C to +8°C

Order information

J12-078 CoolStar Kit . . . . . . . . . . . 2 x 100 ml

J11-007 CoolStar . . . . . . . . . . . . . . . . 100 ml

J11-008 CoolStar Rinse . . . . . . . . . . . . 100 ml

For further details and specifications of: CoolStar see page 96.

CoolStar Rinse see page 103.Cel

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Index

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Alphabetical Index


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Alphabetical Index

AccuMax . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77Accutase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76Alfazyme . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76Amphotericin B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73Antibiotic/Antimycotic Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

B27 NeuroMix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83, 127, 141Blasticidin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87, 119Bovine Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61Bovine Serum Albumin, 30% Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91Bovine Serum Albumin, Fatty Acid Free . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90Bovine Serum Albumin, Fraction V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90Bovine Serum Albumin, low Endotoxin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91Bovine Serum Albumin, Protease Free . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92Bovine Serum, Gamma Irradiated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61Bovine Serum, Heat Inactivated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60Calf Serum, Gamma Irradiated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60Calf Serum, Heat Inactivated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60Carbenicillin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88, 120Colcemid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94, 112Collagen Stem Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35, 135Collagenase 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77CoolStar Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96, 103, 148CoolStar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96, 148CoolStar Rinse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103, 148CryoMaxx I . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95, 147CryoMaxx II . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95, 147CryoMaxx S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95, 146CryoMaxx SF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95, 146

DMEM/Ham's F-12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17DMEM, high Glucose (4.5 g/l) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15DMEM, high Glucose (4.5 g/l), Thermostable Medium . . . . . . . . . . . . . . . . . . . . . . . 16DMEM, low Glucose (1g/l) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14DMEM, low Glucose (1g/l), Thermostable Medium . . . . . . . . . . . . . . . . . . . . . . . . . 16DMEM Ready Mix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28Donor Bovine Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61Donor Bovine Serum, Gamma Irradiated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61Donor Bovine Serum, Heat Inactivated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61Donor Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60Donor Calf Serum, Gamma Irradiated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60Donor Calf Serum, Heat Inactivated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60Donor Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62Donor Horse Serum, Heat Inactivated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62Dulbecco's Phosphate Buffered Saline (DPBS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

Endothelial Basal Medium MCDB 131 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38Endothelial Cell Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38Endothelial Medium Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Foetal Bovine Serum, Amnion cell tested . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55, 111Foetal Bovine Serum, Dialysed, AUS origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54Foetal Bovine Serum, Dialysed, CAN origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54Foetal Bovine Serum, Dialysed, EU approved . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54Foetal Bovine Serum, Dialysed, USA origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

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Foetal Bovine Serum, Dialysed, USDA approved . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54Foetal Bovine Serum, ES cell tested . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55, 137Foetal Bovine Serum, Gamma Irradiated, AUS origin . . . . . . . . . . . . . . . . . . . . . . . . 52Foetal Bovine Serum, Gamma Irradiated, CAN origin . . . . . . . . . . . . . . . . . . . . . . . . 52Foetal Bovine Serum, Gamma Irradiated, EU approved . . . . . . . . . . . . . . . . . . . . . . . 52Foetal Bovine Serum, Gamma Irradiated, USA origin . . . . . . . . . . . . . . . . . . . . . . . . 52Foetal Bovine Serum, Gamma Irradiated, USDA approved . . . . . . . . . . . . . . . . . . . . 52Foetal Bovine Serum, Gold, AUS origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50Foetal Bovine Serum, Gold, CAN origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50Foetal Bovine Serum, Gold, EU approved . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50Foetal Bovine Serum, Gold, USA origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50Foetal Bovine Serum, Gold, USDA approved . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50Foetal Bovine Serum, Heat Inactivated, AUS origin . . . . . . . . . . . . . . . . . . . . . . . . . . 53Foetal Bovine Serum, Heat Inactivated, CAN origin . . . . . . . . . . . . . . . . . . . . . . . . . 53Foetal Bovine Serum, Heat Inactivated, EU approved . . . . . . . . . . . . . . . . . . . . . . . . 53Foetal Bovine Serum, Heat Inactivated, USA origin . . . . . . . . . . . . . . . . . . . . . . . . . . 53Foetal Bovine Serum, Heat Inactivated, USDA approved . . . . . . . . . . . . . . . . . . . . . . 53Foetal Bovine Serum, IgG Stripped, EU approved . . . . . . . . . . . . . . . . . . . . . . . . . . . 54Foetal Bovine Serum, IgG Stripped, USA origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54Foetal Bovine Serum, Mycoplex, AUS origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56Foetal Bovine Serum, Mycoplex, EU approved . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56Foetal Bovine Serum, Mycoplex, USA origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56Foetal Bovine Serum, PAA Clone, EU approved . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51Foetal Bovine Serum, PAA Clone, USA origin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51Foetal Bovine Serum, Pharma Grade . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57, 138Foetal Bovine Serum, Pharma Grade, Gamma Irradiated . . . . . . . . . . . . . . . . . . 57, 138Foetal Bovine Serum, Standard Quality, AUS origin . . . . . . . . . . . . . . . . . . . . . . . . . 51Foetal Bovine Serum, Standard Quality, CAN origin . . . . . . . . . . . . . . . . . . . . . . . . . 51Foetal Bovine Serum, Standard Quality, EU approved . . . . . . . . . . . . . . . . . . . . . . . . 51Foetal Bovine Serum, Standard Quality, USA origin . . . . . . . . . . . . . . . . . . . . . . . . . 51Foetal Bovine Serum, Standard Quality, USDA approved . . . . . . . . . . . . . . . . . . . . . . 51Foetal Bovine Serum, Tetracycline negative, EU approved . . . . . . . . . . . . . . . . . . . . . 56Foetal Bovine Serum, Tetracycline negative, USA origin . . . . . . . . . . . . . . . . . . . . . . 56

G-418 Sulphate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86, 117G-418 Sulphate Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86, 117G5 Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83, 129, 142Gentamicin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74Giemsa Stain Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93, 112Goat Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Ham's F-10 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18Ham's F-12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19Ham's F-10 Ready Mix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32, 110Hanks' Balanced Salt Solution (HBSS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101HematoStem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82, 140HEPES Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62, 140Horse Serum, Heat Inactivated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62Human Serum, converted . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65Human Serum, converted, Type AB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65Human Serum, off the clot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64Human Serum, off the clot, Type AB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64, 139Hybridoma Cloning Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82Hybridoma Express . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40Hygromycin B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88, 118Hygromycin B Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88, 118

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Alphabetical Index

Iscove's modified DMEM (IMDM) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20InsectExpress Sf9-S2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Leibovitz's L-15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21L-Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71L-Glutamine with Penicillin/Streptomycin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72Lymphocyte Separation Medium, LSM 1077 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

Macrophage Medium Mouse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39Medium 199, with Earle's Salts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22Medium 199, with Hanks' Salts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23MEM Alpha Modification, with Ribonucleosides . . . . . . . . . . . . . . . . . . . . . . . . . . . 24MEM Alpha Modification, without Ribonucleosides . . . . . . . . . . . . . . . . . . . . . . . . . 24MEM Amino Acid Concentrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70MEM Vitamins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72MesenchymStem Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37, 136MethoStem Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36, 135Minimal Essential Medium Eagle (MEM) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25Mouse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63MycoKill AB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

N2 Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84, 130, 142Nanofectin Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85, 116NEAA Amino Acid Concentrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70Neo-natal FBS, Gamma Irradiated, USDA approved . . . . . . . . . . . . . . . . . . . . . . . . . 58Neo-natal FBS, Heat Inactivated, USDA approved . . . . . . . . . . . . . . . . . . . . . . . . . . . 58Neo-natal FBS, USDA approved . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58Neuronal Base Medium AD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34, 126Neuronal Base Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33, 125, 136Neuronal Stem Cell Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84, 128, 141Newborn Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59Newborn Calf Serum, Gamma Irradiated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59Newborn Calf Serum, Heat Inactivated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

Penicillin/Streptomycin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74Phytohaemagglutinin (PHA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94, 112Porcine Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63Potassium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93, 112Puromycin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87, 120

Quantum 101, Complete HeLa Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43Quantum 263, Complete Tumour Cell Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44Quantum 286, Complete Epithelial Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45Quantum 3-21, Complete Amnion Medium . . . . . . . . . . . . . . . . . . . . . . . . . . 30, 108Quantum 333, Complete Fibroblast Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46Quantum PBL, Complete Medium for Peripheral Blood Lymphocytes . . . . . . . . . 31, 109

Rabbit Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63Rat Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63RPMI 1640 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26RPMI 1640 Ready Mix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29RPMI 1640 Thermostable Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

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Sheep Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63Sodium Bicarbonate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102Sodium Pyruvate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92Stable Glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

TC 100 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42Trypsin (1:250) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78Trypsin (1:250), UV Inactivated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81Trypsin EDTA (1:250) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79Trypsin EDTA (1:250), UV Inactivated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

Water, AP Grade . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103Water, AP Grade in sterile container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

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