CELL CULTURE AND CELL BIOLOGY

OUR BRANDS

101Bio, Bertin BioReagent, Biotium, Canvax, Cayman, Corning, Corning Media, Eurobio, Fal-con, Innovative Cell Technologies, PAN-Biotech, Polyplus transfection, Polysciences, Prospec, ScienCell and many more...

3D cell culture

¸ Hydrogels for 3D cell-culture → Matrigel® matrix → Col-TGel (101Bio): collagen based gel without growth factors and

with 3 different stiffness to fit most cell types

¸ Porous membranes → Transwell® inserts and HTS Plates → Alvetex scaffold: natural tissue-like structure that enables the cells

to function in a more physiologically relevant pattern

¸ Ultra Low Attachment (ULA) microplates: 3D spheroid formation and generation of a single 3D uniform spheroid per well with reproducible size

Dear customer,

discover our large product range for your Cell Culture & Cell Biology product needs. Our icon meanings:

Information about additional documentation

Order your sample at sample@brunschwig-ch.com

; You can now download your certificate of analysis on our website under Support

Your Chemie Brunschwig AG Team

FBS and Sera

¸ Classical Fetal Bovine Sera → several origins (South America, USA, Australia, …) → different treatments (i.e. tet-negative, ultra low IgG, heat-inactivated, dialyzed, delipidized, …)

¸ FBS Supreme, standardized Fetal Bovine Serum: single components naturally present in the serum are enriched in a higher quality leading to a more defined product; time consuming for batch testing would not be necessary anymore.

¸ FBS Active, in this product 80% of the FBS is replaced by chemically defined components. The reproducibility of your cell culture experiments or processes can be improved tremendously.

¸ Panexin Basic & Panexin NTA are fully defined serum replacement for the cul-tivation of adherent and non-adherent cells. Support the growth of many cell types in an optimum manner without any extra handling compared to serum.

¸ Filtrated Bovine Serum, a cost effective alternative to FBS suitable for many cells types ¸ Human and animal Sera

Animal free systems

¸ Serum free media → for different cell types (HEK-293, CHO, keratinocytes, Vero cells, lympho-

cytes, hybridoma cell lines,…) → for routine cultures or bioprocesses (protein, antibody or virus production)

¸ Panexin CD is a full chemically defined new serum replacement for the cultiva-tion of adherent and non-adherent cells that shows a high reproducibility from lot to lot and simplified downstream processing.

¸ Human platelet lysate (hPL) is a xeno-free alternative to FBS as well as human serum for in vivo wound healing and tissue repair, but also for the expansion of human cells in vitro.

see & download PAN catalog 2018-2019 on website under suppliers

Media, Supplements, Buffers & Reagents

¸ Broad range of classical media (DMEM, RPMI, Ham’s F-12, …) → in liquid or powder form → various formulations and pack sizes

¸ All reagents and buffers for cell culture ¸ Cell dissociation

→ classical enzymes (Collagenase, Trypsin) → Accutase® a smooth alternative to trypsin for sensitive cells, FACS or receptor studies

Free sample available - ask yours! ¸ Growth factors: hormones, cytokines, chemokines: human or animal ¸ Cell specific media: basal media or kits incl. media + pre-tested FBS + growth suppl. ¸ Customized media

Cells and Tissues

Transfection

¸ DNA transfection reagent: JetOPTIMUS®

→ high efficiency → cost-effective → also suitable for hard-to-transfect-cells

¸ siRNA/miRNA transfection reagent: INTERFERin®

→ high Silencing efficiency → low off-target effects

¸ mRNA transfection reagent: jetMESSEGER®

→ unmatched transfection efficiency → extremely gentle on the cells

¸ RNP (Cas9 protein+gRNA) transfection reagent: jetCRISPR®

→ high genome editing efficiency → maintained cell viability

¸ in-vivo nucleic acid delivery: In-vivo JetPEI®

→ easy and safe delivery method → convenient to any animal model

¸ Bioproduction: PeiPRO, FectoCHO/PRO®

→ Protein production → Virus production

DNA

mRNA

Ribonucleoprotein (RNP)

jetOPTIMUS® – DNA method• Plasmid DNA encoding both Cas9 and

gRNA

jetMESSENGER® - RNA approach• mRNA encoding Cas9 and gRNA

jetCRISPR® - RNP Delivery• Cas9 protein complexed with gRNA

¸ Cells → Human and animal primary cells, fresh or frozen suitable

for R&D, drug discovery, pharmacology & toxicology ¸ Hepatocytes

→ Fresh or cryopreserved → Human and Animal → HepaRG™, the human hepatic stem cell line

¸ Human and animal tissues → Skin Tissues → cells and fractions for permeability, toxicology or

metabolism research

¸ Biologic fluids

¸ SILENSOMES One single model for DMPK phenotyping studies

Supplies for Laboratory and Prodcution

Chemie Brunschwig AGAuf dem Wolf 10CH-4052 Basel

T +41 61 308 91 11info@brunschwig-ch.comwww.brunschwig-ch.com

Cell biology assays

¸ Viability Study: Resazurin, MTT, XTT, ATP-Glo ¸ Apoptose: Caspase, Annexin, TUNEL assays ¸ Reporter gene assays: firefly/renilla luciferase assays, Galactosidase assays,

SEAP reporter gene assays ¸ Metabolite quantitation Kits: cholesterol, free fatty acids, triglycerides, … ¸ Stress oxidative assays ¸ ELISA kits: Ready-to-Use or Do-It-Yourself kits ¸ Organel studies:

→ mitochondries (MitoView, JC-1, …) → nucleus (DAPI, Red-Dot, Hoechst dyes, …) → lysosome (LysoView) → cytoskelet (Phalloidins)

¸ Fluorescent ion and pH indicators ¸ Membrane potential dyes

Disposable labware

¸ High-quality disposable plastic ware for cell culture → Flasks, dishes, microplates, scrapers, … → Different treatments available for adaptability to any cell types: Not-treated, TC-treated,

Ultra Low Attachement, pre-coated ¸ Liquid handling

→ pipettors, pipet controller, aspirators, plate washer, serological pipets, filtration systems, centrifuge tubes, bottles, sterile racked filter tips,…

¸ Plastic ware for Bioprocess → Erlenmeyer (from 125ml to 5L), stackable cell culture flasks (up to 25‘440cm2), Roller bottles,

Bioreactors (from 50ml to 36L), Microcarriers for bioprocess scale-up: available with 4 surface treatments

¸ Cryogenic vials and accessories → cryogenic vials with external or internal thread, racks and storage boxes, CoolCell, CoolBox and

CoolRacks for a consistent and reproducible cooling or temperature control ¸ Cell Strainers for cell isolation from tissues ¸ Benchtop equipment

→ centrifugation equipment (micro- or mini-centrifuge, high speed micro centrifuge, compact centrifuges, …)

→ vortex mixers, platform or 3D shakers, Tube roller or rotator, Magnetic & hotplate stirrers

→ Cell Counter

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