cell cryopreservation biocision 2015

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Presented by: Michelle Nemits September 2015

Standardizing the Cell Cryopreservation Workflow with Eco-Friendly Solutions

• BioCision overview• The cell cryopreservation workflow

> Areas of potential variability and contamination

• Integrating BioCision products > Reduce variability and improve reproducibility> Reduce water, energy and solvent usage

Today’s topics

2Copyright 2015. All rights reserved.

Who is BioCision?• Founded in 2007 by drug discovery and biomedical engineering experts• Developing unique solutions to address variability in

temperature- sensitive workflows


Cell cryopreservationCryopreservation is the use of low temperatures to preserve structurally intact living cells to avoid loss by contamination, to minimize genetic change in continuous cell lines and to avoid transformation in finite lines.It is critical to employ standardized procedures that minimize risk of variability and contamination.


Cell cryopreservation workflowHighlighting areas susceptible to variability and contamination

Slowly freeze cells at

-1°C/min rate in ULT freezer

Freeze.

Remove frozen vials from ULT

freezer to long-term storage

Transport.

Store at ULT or cryogenic

temperature

Store.

Retrieve vials from storage

and thaw

Thaw.

Prepare media & cryoprotectant;

add to cells; dispense cell

suspension into vials

Prep.

Solvent-based cell freezingIPA-based containers can introduce variability, require on-going solvent change and disposal

Dry ice & liquid nitrogen transportLack of hand-portable transport solutions that ensure stable temperature control

Water bath thawingHigh risk of contamination, technique-dependent, subjective, and variable endpoints

Cooling with iceIce cannot be used in the hood and is potential source of contamination and variability

Standard cryoboxesLid and base can be separated, labels mixed up, white/beige makes it difficult to identify quickly


CoolBox™ Ice-free cooling

CoolCell® Alcohol-free cell

freezing

CryoPod™

Portable temp stabilityTruCool®

Hinged cryoboxesThawSTAR™

Automated cell thawing

Prep. Freeze. Transport. Store. Thaw.

BioCision products standardize and reducevariability at each step……

… while also reducing water, electricity and solvent usage6

Copyright 2015. All rights reserved.

Prep: CoolBox™ XT ice-free cooling systemsPrep.

Problem • 0-4°C cooling – without ice, electricity, batteries

• Lasts 10 hours with lid open, 16 hours closed• Well-to well sample uniformity• Ideal for use in cell culture hood• 100 lbs. / day of ice = over 5,000 gallons water(1,2)

and 3,000 kWh of electricity(3) annually7


Freeze: CoolCell® alcohol-free cell freezingFreeze.

Problem

Five consecutive freeze runs show nearly identical thermal profiles

• Isopropanol-free cell freezing -1°C/minute freezing

> Eliminates up to 12L of solvent per unit per year

• Proven for stem cells, primary cells, PBMCs, cell lines, yeast and others 8


Transport: CryoPod™ Carrier cryogenic transportTransport

• 3L of LN2 provides over 4 hours of < -150°C cooling

• Hand-portable, < 20lbs when loaded• No direct contact with LN2

• Optional automated filling station

Basket top2.5 inches from bottom2 inches from bottomBasket bottomProble

m


Store: TruCool™ hinged cryostorage boxesStore.

Problem

• Hinged lid keeps base and lid together• Laminated coating & plastic divider provides

durability• 6 bright colors - facilitates quick in/out of

freezer, reducing strain on compressor10


Thaw: ThawSTAR™ automated cell thawingThaw.

Problem • Solid-state, algorithm-driven technology

standardizes thawing endpoints• Proven high reproducibility & post-thaw cell

viability for stem cells, PBMC, cell lines, others

Data courtesy of Blood Systems Research Institute

• Replaces water bath method saving 100L to 1500L water per year per bath(4)


Easy to integrate. Easy to use.

Standardize your workflowwhile reducing water, energy and solvent

consumption

CoolBox™ Ice-free cooling

CoolCell® Alcohol-free cell

freezing

CryoPod™

Portable temp stabilityTruCool®

Hinged cryoboxesThawSTAR™

Automated cell thawing

Prep. Freeze. Transport. Store. Thaw.


Contact us at [email protected]

Thank you.References:(1) US Dept of Energy, Energy Cost Calculator for Commercial Ice Machineshttp://energy.gov/eere/femp/energy-cost-calculator-commercial-ice-machines-0#output(2) Commercial Ice Machine Life-cycle Cost Calculator, Bill Hoffman, P.E., City of Austin TX Water Conservation Program Telephone: (512) 974- 2893 Email: [email protected](3) Alliance for Water Efficiency, Promoting the Efficient and Sustainable Use of Waterhttp://www.allianceforwaterefficiency.org/Ice_Machines.aspx(4) Based on web search of ShelLabs water bath sizes – 2L to 30L http://shellab.com/product-category/water-baths-c-298/

Copyright 2015 BioCision, LLC. All rights reserved. CoolBox, CoolCell, TruCool, ThawSTAR designations are trademarks owned by BioCision, LLC. CryoPod is a trademark of Brooks Automation.

cell cryopreservation biocision 2015

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