cell based reporter assays vs

8/3/2019 Cell Based Reporter Assays Vs

1/11

Yiaicary*y Lcoj

Kmt|aky &\YY!

Ioggkmty &\YY!

Fogk

Nlo{t

Yiaicary Iomy{ctnmiq Yk|uaiky

Ikcc Lnykd \kro|tk| Nyynqy uy- Nmagnc Yt{daky am

D|{j Dayiouk|q' Rotkmtanc Cagatntaomy# \ayhy

nmd Canlacataky

Roytkd lq0 yiaicary om0 @nm{n|q >5# 5/ikcc'lnykd'|kro|tk|'nyy

> >/


2/11

Ikcc lnykd |kro|tk| nyynqy tfnt n|k gn|hktkd eo| d|{j dayiouk|q nyynqy n|k oetkm r|o`kitkd ny nm nctk|mntauk to

nmagnc lnykd yt{daky# zatfo{t zkcc'r|oukm yiakmtaeai lnyay to y{rro|t tfkyk icnagy- Tfk nyynq r|amiarck nmd tfk

gktfody nrrkn| to lk yiakmtaeainccq nrrkncamj ae at ay mot y{l`kitkd to am'dkrtf o| iogr|kfkmyauk yiakmtaeai

nmncqyay# zfaif ay oetkm ajmo|kd o| mot iomyadk|kd ny n enito| tfnt gnq fnuk comj'tk|g iomyk~{kmiky am nmq

d|{j dayiouk|q/dkukcorgkmt yt{daky- Tfk|k n|k ykuk|nc hkq ayy{ky zatf ikcc lnykd |kro|tk| nyynqy# yogk oe

zfaif zk fnuk nc|kndq ndd|kyykd am kn|cak| lcojy- Jkmktainccq godaeakd ikccy {ykd am ikcc lnykd nyynqy inm lk

daeek|kmt e|og rn|kmtnc ikccy# r|agn|acq d{k to jkmktai nmd rfqyaocojainc nctk|ntaomy |ky{ctkd e|og comj'tk|g

ikcc i{ct{|k iomdataomy nmd eo|ikd kvr|kyyaom oe |kro|tk| jkmk/r|otkamy# oetkm zatf f{gnm o| mom'f{gnm

r|otkam e{yaom rn|tmk|y- Comj tk|g i{ct{|ky inm amd{ik g{tntaomy nmd eo|ikd kvr|kyyaom oe |kro|tk|jkmky/r|otkamy inm nctk| tfk ikcc yajmncamj rntfznqy# zfaif gnq |ky{ct am encyk royatauk/mkjntauk |ky{cty- Tfk

jkmktai nmd krajkmktai agrnit oe |nmdog amtkj|ntaom oe |kro|tk| jkmky# inm un|q lnykd om tfk amtkj|ntaom yatk

o| iorq m{glk| oe amtkj|ntkd |kro|tk| jkmky o| fktk|ojkmkatq &lotf jkmktai nmd krajkmktai! oe y{if ikccy# n|k

oetkm mot tfo|o{jfcq yt{dakd o| {mdk|ytood am ikcc lnykd |kro|tk| nyynqy- \nmdog amtkj|ntaom oe |kro|tk|

jkmky# lotf am iodamj nmd mom'iodamj |kjaomy# inm nctk| o| yackmik ik|tnam jkmky o| neekit jkmktai |kj{cnto|q

gkifnmayg# nmd tfk |ky{ctamj ikcc camky gnq mot lk {maeo|g eo| y{if jkmktai o| krajkmktai ifnmjky- Am

nddataom# daeek|kmtanc kvr|kyyaom oe gai|o\MN &ga\MN! o| un|antaomy am gktnlocatk rntfznqy# zfaif rcnq

yajmaeainmt |ock am ikcc{cn| |kj{cntaom nmd yajmncamj r|oikyy# n|k mot zkcc yt{dakd am jkmktainccq godaeakd ikcc

camky zatf |kro|tk| jkmky- Tfk nlouk'gkmtaomkd jkmktai nmd mom'jkmktai enito|y# yogk oe zfaif fnuk lkkm

dayiouk|kd am |kikmt qkn|y# inm lk gn`o| ifncckmjky am dkukcoramj ikcc lnykd |kro|tk| nyynqy- Fozkuk|# ae ikcc

lnykd |kro|tk| nyynqy n|k {ykd am d|{j dayiouk|q yt{daky zatfo{t {mdk|ytnmdamj tfkyk i|atainc enito|yy{iikyye{c d|{j dayiouk|q/dkukcorgkmt r|oikyy inm lk yiakmtaeainccq ifncckmjamj# ioyt'r|ofalatauk nmd

tagk'iomy{gamj-

At inm lk kukm zo|yk ae ikcc lnykd |kro|tk| nyynqy n|k lnykd om mom'mnt{|nc |kro|tk| r|otkamy y{if ny ia|i{cn|cq

rk|g{tkd ea|kecq c{iaek|nyk o| JER lnykd |kro|tk| r|otkam- Ia|i{cn| rk|g{tntaom ay iomyadk|kd to lk nm kc{yauk

gocki{cn| kuoc{taom r|oikyy tfnt amuocuky jkmk d{rcaintaom o| kvom yf{eecamj nmd ia|i{cn|cq rk|g{tkd r|otkamy

gnq mot lk mnt{|nccq r|kykmt am gnggncanm ikccy- Uk|q ekz mnt{|nccq oii{||amj ia|i{cn|cq rk|g{tkd r|otkamy

fnuk lkkm |kro|tkd am lnitk|an nmd am rcnmty &k-j- ifco|orcnyt nyyoiantkd lnitk|anc fogocoj r|otkam!- Lnykd om

tfkyk yiakmtaeai kuadkmiky# tfk |kcanlacatq oe ia|i{cn|cq rk|g{tkd ea|kecq c{iaek|nyk lnykd |kro|tk| nyynqy mkkdy

to lk in|ke{ccq nmncqpkd yamik am uauo kvr|kyyaom oe ia|i{cn|cq rk|g{tkd r|otkamy am ikccy gnq cknd to

yajmaeainmt ifnmjky am jkmktai |kj{cnto|q gkifnmayg &k-j- t|nmycntaomnc gnifamk|q!# ikcc{cn| gktnlocayg#ikcc{cn| rfqyaocojq nmd ikcc yajmncamj rntfznqy- Tfk ~{kytaom ay# inm zk |kcq om mom'mnt{|nc |kro|tk| r|otkam

lnykd ikcc lnykd nyynqy am d|{j dayiouk|q o| dkukcorgkmt? Tfo{jf tfk d|{jy tfnt n|k amatanccq ykckitkd {yamj

y{if ikcc lnykd |kro|tk| nyynqy do mot |knif {r to f{gnm icamainc t|ancy# tkytamj y{if kvrk|agkmtnc d|{jy am

nmagncy gnq royk canlacataky- Tfk oluao{y |knyom ay# tfkyk d|{jy n|k yi|kkmkd nmd ykckitkd {yamj mom'mnt{|nc

|kro|tk| r|otkam kvr|kyyamj ikccy# zfaif gnq lk nlmo|gnc ikccy- Yamik tfk mnt{|nc jkmktai gnifamk|q oe mo|gnc

ikccy gnq mot tock|ntk y{if mom'mnt{|nc r|otkamy# eo|ikd kvr|kyyaom oe tfkyk r|otkamy am mo|gnc ikccy gnq |ky{ct

am jkmktai o| krajkmktai nlmo|gncataky# zfaif gnq cknd to nmognco{y ikcc yajmncamj o| gktnlocai rntfznqy-

Kvtkmyauk yiakmtaeai uncadntaom oe tfkyk ikccy o| ikcc camky ay |k~{a|kd# r|ao| to nmq nmagnc tkytamj# to ndd|kyy tfk

laocojainc |kckunmik oe tfk nyynq nmd laocojainc o| jkmktai o| tovaiatq k~{aunckmik to mo|gnc ikccy- Zk do mot

day|kjn|d tfk enit tfnt tfk|k n|k ykuk|nc |kro|ty om tfk {yk eo| ia|i{cn|cq rk|g{tkd r|otkamy eo| agr|ouamj

kmpqgk ytnlacatq o| r|otknyk |kyaytnmik kti- L{t# tfkyk r|otkamy zk|k n|taeaianccq yqmtfkyapkd# kvr|kyykd nmdr{|aeakd {yamj |kioglamnmt gktfody eo| e{mitaomnc o| yt|{it{|nc yt{daky- Om tfk iomt|n|q# am d|{j

dayiouk|q/dkukcorgkmt nrrcaintaomy ikcc lnykd ia|i{cn|cq rk|g{tkd |kro|tk| r|otkamy n|k lkamj {ykd eo|

nmncqpamj nmd r|kdaitamj i|atainc d|{j'amd{ikd o{tiogky {yamj am uauo yt{daky-

Tfk t|{tf ay tfnt i{||kmt ikcc lnykd |kro|tk| nyynqy dad mot ndnrt o| kuocuk am rn|ncckc zatf |kikmt yiakmtaeai

dayiouk|aky# zfaif gnq fnuk yajmaeainmt agrnit om ikcc'lnykd nyynq o{tiogky- Amtkj|ntkd g{ctadayiarcamn|q

iomyadk|ntaomy n|k mkkdkd to dkukcor keeaiakmt ikcc lnykd nyynqy- Tfk|k n|k ykuk|nc tkifmocojaky i{||kmtcq

nunacnlck eo| nmncqpamj tfk jkmktai# r|otkogai# krajkmktai# ga\MN o| gktnlocatk ifnmjky am jkmktainccq

godaeakd |kro|tk| ikcc camky- Nt cknyt# yogk oe tfkyk nmncqyky mkkd to lk domk lkeo|k zk {yk ikcc lnykd

Lnykd \kro|tk| Nyynqy uy- Nmagnc Yt{daky am D|{j Dayiouk|q' Rotkmt--- fttr0//yiaicary-zo|dr|kyy-iog//>5/ikcc'lnykd'|kro|tk|'nyy

> >/


3/11

|kro|tk| nyynqy am d|{j dayiouk|q/dkukcorgkmt yt{daky- Am iomt|nyt# kgk|jamj tkifmocojaky cahk mom'amunyauk

agnjamj oe gktnlocai ifnmjky am tayy{ky o| zfock nmagncy {yamj \ngnm yrkit|oyiorq cooh uk|q r|ogayamj am

d|{j dayiouk|q |kykn|if- Ammountaomy am yagacn| mom'amunyauk tayy{k o| nmagnc/f{gnm lnykd tkifmocojaky#

zfaif do mot amuocuk jkmktai godaeaintaom oe ikccy o| tayy{ky# gnq orkm {r mkz nukm{ky am dkukcoramj

nctk|mntauk tkifmocojaky to ikcc lnykd |kro|tk| nyynqy-

Tfk {tacatq oe ytkg ikcc lnykd d|{j dayiouk|q nyynqy inm lk gayckndamj?

Ytkg ikccy n|k oetkm r|o`kitkd ny nm nctk|mntauk ikcc lnykd yqytkg eo| d|{j tovaiatq# in|daotovaiataq kti- Zkfnuk ndd|kyykd tfk cagatntaomy oe ytkg ikcc lnykd nyynqy am d|{j dayiouk|q am o{| kn|cak| lcojy- Fk|k zk zo{cd

cahk to ndd|kyy zfktfk| i{||kmtcq {ykd o| r|oroykd ytkg ikcc lnykd nyynqy inm lk nm nctk|mntauk to nmagnc

godkc yt{daky- O{| nmyzk| zo{cd lk mo- Cahk nmq jkmktainccq godaeakd ikccy# ytkg ikccy n|k ncyo r|omk to

jkmktai# krajkmktai nmd rfqyaocojainc ifnmjky- Zatfo{t {mdk|ytnmdamj tfk rfqyaocojainc nmd jkmktai/krajkmktai

iomyk~{kmiky oe ytkg ikcc lnykd nyynqy# amic{damj ytkg ikcc lnykd |kro|tk| nyynqy# nmq tkytamj oe d|{jy

ykckitkd {yamj y{if nyynqy gnq fnuk nduk|yk iomyk~{kmiky am nmagnc yt{daky- Fozkuk|# ytkg ikccy inm lk n

rotkmtanc nctk|mntauk to nmagnc yt{daky# r|ouadkd tfnt ytkg ikcc lnykd d|{j dayiouk|q nyynqy n|k dkukcorkd zatf

yo{md yiakmtaeai r|amiarcky zfk|k i|atainc nmd e{mdngkmtnc ~{kytaomy n|k ndd|kyykd nmd yocukd8 zatf in|ke{c

iomyadk|ntaom oe comj'tk|g agrnit- At yfo{cd ncyo lk motkd tfnt mo|gnc ikcc lnykd nyynqy gnq mot lk nm adknc

tooc eo| r|kdaitamj nduk|yk d|{j |knitaomy &ND\!# zfaif gnq |k~{a|k nmagnc yt{daky- Fozkuk|# ytkg ikcc

lnykd nyynqy io{rckd zatf ikcc/tayy{k'yrkiaeai daeek|kmtantaom gnq oeek| {ma~{k toocy eo| r|kdaitamj ND\#zfaif inm lk ifn|nitk|apkd tfo{jf jkmktai# krajkmktai# r|otkogai o| gktnlocogai nmncqyay- Am o|dk| to

nifakuk tfkyk joncy# tfk yiakmtaeai hmozckdjk jkmk|ntkd e|og lnyai dayiouk|aky mkkdy to lk tkytkd nmd

uncadntkd yiakmtaeainccq eo| dkukcoramj tkifmainccq iogrktkmt nmd ioggk|ianccq uanlck d|{j dayiouk|q toocy

tfnt inm lk {ykd eo| dkukcoramj ynek| nmd ioyt'keekitauk d|{jy- Nmq keeo|ty am dkukcoramj ioggk|ianccq

enyt't|nih ytkg ikcc lnykd nyynqy lnykd om yrki{cntauk o| mnyikmt nyy{grtaomy# r|o`kitkd e|og amda|kit

kvrk|agkmtnc |ky{cty# zacc lk yiakmtaeainccq gayckndamj nmd ioggk|ianccq {my{iikyye{c am tfk comj'tk|g-

Amtkcckit{nc r|ork|tq &AR! |ajfty inm lk n |ond lcoih to ammountaomy am ikcc lnykd nyynqy tfnt inm |krcniknmagnc yt{daky?

Tfk rntkmt yikmn|ao am ikcc lnykd nyynqy inm lk n |knc |ond lcoih to e{|tfk| ammountaomy am tfay n|kn- Tfk gn`o|cagatntaomy n|k tfk nunacnlacatq oe ikcc lnykd nyynqy to tfk i{ytogk|y lkin{yk oe tfk amtkcckit{nc r|ork|tq &AR!

|ajfty# n yajmaeainmt enito| tfnt neekity tfk dkukcorgkmt oe agr|oukd o| y{rk|ao| r|od{ity- Kvikrtaomy n|k i|oyy

caikmyamj# zfaif atykce ay n uk|q iogrcaintkd r|oikyy fknuacq nyyoiantkd zatf l|nmd mngk# r|oeatnlacatq nmd

gn|hkt yfn|k- I{ytogk|y n|k oetkm cket zatf cagatkd ifoaiky oe {yamj ik|tnam nyynqy# zfaif n|k rntkmtkd zatf

yiakmtaeainccq ~{kytaomnlck l|ondk| icnagy nmd nrrcaintaomy- Oetkm# Icnagy nmd Kvrk|agkmty

|ky{cty/olyk|untaomy am rntkmty inm lk yiakmtaeainccq {m|kcntkd- Zatf |kyrkit to r|od{it kmd'{yk| caikmyk# tfk

{yk oe |knjkmty ay ncyo |kyt|aitkd to yrkiaeai ikcc lnykd nyynq r|od{ity# kukm tfo{jf yagacn| y{rk|ao| |knjkmty

gnq lk nunacnlck am tfk gn|hkt- Tfk AR |ajfty gnq ncyo cknd to kvtkmdkd caikmyamj nj|kkgkmt tfnt inm jkmk|ntk

|oqnctq e|og d|{jy dayiouk|kd tfo{jf ik|tnam ikcc lnykd nyynqy# amic{damj ytkg ikcc lnykd nyynqy- Tfk ~{kytaom

ay# ae n d|{j enacy d{k to nduk|yk d|{j |knitaomy &ND\! am rntakmty# zfktfk| tfk gnm{enit{|ky oe ikcc lnykd

|kro|tk| nyynqy n|k ncyo |kyromyalck eo| tfk canlacataky yagacn| to tfk d|{j gnm{enit{|k|? A||kyrkitauk oe tfkyk AR|kcntkd ayy{ky# ikcc lnykd nyynqy tfnt n|k gn|hktkd zatfo{t iomyadk|amj tfk yiakmtaeai gk|aty nmd rotkmtanc

comj'tk|g |ayhy nmd canlacataky am nmagnc o| f{gnm yt{daky gnq fnuk to lk in{tao{ycq {ykd am d|{j

dayiouk|q/dkukcorgkmt- Ncyo# tfk {yk oe ik|tnam ikcc lnykd |kro|tk| nyynqy# cahk tfk mom'mnt{|nc |kro|tk|

r|otkamy# am r{lcainccq e{mdkd d|{j dayiouk|q r|o`kity mkkdy to lk |kkunc{ntkd lkin{yk tfk yiakmtaeai uncadatq

oe tfkyk nyynqy n|k mot ickn|cq dkgomyt|ntkd- Y{if niio{mtnlacatq gnq cknd to tfk jkmk|ntaom oe rntkmty zatf

yiakmtaeainccq uncad icnagy tfnt n|k kytnlcayfkd tf|o{jf yt|omj yiakmtaeai nmd kvrk|agkmtnc dkyajm nmd nmncqyay-

Tfay nrr|onif gnq {ctagntkcq fkcr am dkukcoramj ammountauk ikcc lnykd d|{j dayiouk|q/dkukcorgkmt nyynq

r|od{ity o| yagacn| tkifmocojaky tfnt inm |krcnik# nt cknyt rn|t# kvtkmyauk nmagnc yt{daky-

Zfnt n|k tfk yoc{taomy?


> >/


4/11

Yfn|k tfay0

Cahk tfay0 Omk lcojjk| cahky tfay royt-

Tfk r|agn|q jonc oe tfay lcoj ay mot to eamd o| y{jjkyt n yoc{taom# |ntfk| to ndd|kyy tfk ayy{ky tfnt n|k mot oetkm

dayi{yykd8 nmd to i|kntk yiakmtaeai nzn|kmkyy nmd eniacatntk yiakmtaeai iomykmy{y to dkukcor ammountauk ikcc

lnykd nyynq toocy- Zk lkcakuk agroyamj niio{mtnlacatq# tajftk| |kj{cntaomy nmd dkgnmdamj fajfk| ytnmdn|dy am

ikcc lnykd nyynqy gnq cknd to tfk dayiouk|q oe ammountauk ikcc lnykd toocy tfnt inm {ctagntkcq |krcnik nmagnc

godkc yt{daky# nt cknyt am rn|t-

\kcntkd lcojy0

>- Inmik| Tfk|nmoytaiy Rotkmtanc Nrrcaintaomy oe Inmik| Laogn|hk| Dntnlnyk- D|{j dayiouk|q r|otoiocy51>>156# < nt 90 ng


> >/


5/11

Zk n|k t|qamj to adkmtaeq nmd i|kntk n cayt oe tkifmocojaky o| r|od{ity |kcntkd to d|{j dayiouk|q/dkukcorgkmt

nyynqy# zfaif do mot amuocuk jkmktai gnmar{cntaom oe ikccy o| |kro|tk| jkmky/r|otkamy o| g{ctakmpqgk

dkrkmdkmt |kndo{t- Tfk nyynqy amic{dk lotf am uat|o nmd am uauo JRI\# jkmk|nc |kikrto|y# iNGR# r|otknyk#

m{ickn| fo|gomk |kikrto|y# aom ifnmmkc r|otkamy# r|otkam hamnyky# RA9'Hamnyky kti- Rcknyk fkcr {y am

adkmtaeqamj tfkyk tkifmocojaky o| r|od{ity lq yfn|amj qo{| ameo|gntaom zatf {y nt iomtnitByiaicary-iog-

\krcq

9 s yiaicary

@nm{n|q 5/ikcc'lnykd'|kro|tk|'nyy

> >/


6/11

Motaeq gk oe eoccoz'{r ioggkmty uan kgnac- Royt Ioggkmt

Ykn|if

Iogr|kfkmyauk Inmik| Logn|hk| Dntnlnyk

Yiaicary iomy{ctnmiq


> >/


7/11

Danjmoytai % R|ojmoytai Laogn|hk| Dntnlnyk

Laor|otoioc dntnlnyk &orkm niikyy!

Ioglamntaom tfk|nrq dntnlnyk &orkm niikyy!

Tfk|nrk{tai d|{j tn|jkt dntnlnyk &orkm niikyy!

Laoameo|gntaiy dntnlnyky &orkm niikyy!

Ytkg ikcc |kykn|ifk|y dntnlnyk

N iogr|kfkmyauk dntnlnyk eo| ytkg ikcc |kykn|ifk|y# Ytkg ikcc |kykn|if |knjkmty# Ytkg ikcc rntkmty# Ytkg ikcc

j|nmty# Ytkg ikcc icamainc t|ancy# Ytkg ikcc ytntaytaiy# Ytkg ikcc mkzy nmd Ytkg ikcc orkm ammountaom adkny- Rcknyk

uayat fttr0//zzz-yiaicary-iog/


> >/


8/11


R|otkogaiy |kykn|ifk|y dntnlnyk

N iogr|kfkmyauk dntnlnyk eo| r|otkogaiy |kykn|ifk|y# R|otkogaiy |kykn|if |knjkmty# R|otkogaiy rntkmty#

R|otkogaiy j|nmty# R|otkogaiy icamainc t|ancy# R|otkogaiy ytntaytaiy# R|otkogaiy mkzy nmd R|otkogaiy orkm

ammountaom adkny- Rcknyk uayat fttr0//zzz-yiaicary-iog/

Orkm Ioccnlo|ntaom

fttr0//zzz-yiaicary-iog/yiaicary/orkmIoccnlo|ntaom-do

Yfn|k o| ykcc |kykn|if |knjkmty r|krn|kd am qo{| cnlo|nto|q

fttr0//zzz-yiaicary-iog/yiaicary/yfn|k\kykn|if\knjkmty-do?kvrnmdnlck72

N|ifauky

@nm{n|q

Oitolk| >

Ykrtkglk| >

N{j{yt >

@{cq >

Gnq >

Gn|if >

Dkikglk| 2

Oitolk| 2

Ykrtkglk| 2@{mk 2

Nr|ac 2

Ekl|{n|q 2

Dkikglk| >/


9/11

Rfn|gniojkmogaiy r|otoiocy omcamk

fttr0//zzz-yiaicary-iog/yiaicary/lao'r|otoiocy-do

Ytkg ikcc r|otoiocy omcamk


R|otkogaiy nmd Gnyy yrkit|ogkt|q &GY! r|otoiocy omcamk


Tfk|nrk{tai d|{j tn|jkty dntnlnyk &orkm niikyy!

D|{j dayiouk|q# Laorfn|gnik{taincy# FTY nyynqy r|otoiocy omcamk


R|otkam dayrcnq/R|otkam kmjamkk|amj/Da|kitkd kuoc{taom r|otoiocy omcamk


Gocki{cn| laocojq nmd Ikcc laocojq r|otoiocy omcamk


Krajkmktaiy R|otoiocy omcamk



> >/


10/11

ya\MN r|otoiocy ga\MN r|otoiocy omcamk


Uadko r|otoiocy

Ikcc i{ct{|k uadko r|otoiocy# Iqtotovaiatq nyynq uadko r|otoiocy# Ytkg ikcc uadko r|otoiocy# Gocki{cn| laocojq

uadko r|otoiocy fttr0//zzz-yiaicary-iog/yiaicary/uadko'r|otoiocy-do


\kikmt Ioggkmty

Ncnm Ioccay om Ikcc Lnykd \kro|tk| Nyynqy uy-

yiaicary om Ikcc Lnykd \kro|tk| Nyynqy uy-

Yiaicary om Ikcc Lnykd \kro|tk| Nyynqy uy-

|mi>> >/


11/11

Laor|otoiocy omcamk &orkm niikyy!

Ioglamntaom tfk|nrq dntnlnyk &orkm niikyy!

Tfk|nrk{tai d|{j tn|jkty dntnlnyk &orkm niikyy!

Laoameo|gntaiy dntnlnyky &orkm niikyy!

FTY nyynq r|otoiocy omcamk &orkm niikyy!

Lcoj nt Zo|dR|kyy-iog-

Tfkgk0 Nclko lq Dkyajm Dayknyk-


cell based reporter assays vs

Documents