cbs isothermal -190c freezer

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Custom BioGenic Systems Inc.

Founded in 1987 Custom BioGenic Systems Inc.,

manufactures and sells state of the art cryopreservation

freezers and full inventory control systems.

In the year 2000 Custom BioGenic Systems Inc.,

patented the first -196°C dry liquid nitrogen storage

system.

Today Custom BioGenic Isothermal Freezers are

known worldwide for their ability to safely store a wide

range of samples at liquid nitrogen temperatures without

liquid nitrogen contact.

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Custom BioGenic Systems World Headquarters in Bruce Twp., Michigan.

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CBS Isothermal -196°C dry storage freezers

CBS Standard liquid nitrogen freezers

Cryosystems, vapor-shippers and lab tanks

Controlled rate freezers

Electronic controls, alarms and data recorders

Freezer racks and inventory systems

Custom BioGenic Systems manufactures a

wide range of products for industries that

include; life sciences, research laboratories,

veterinarian, horticulture, pharmaceutical,

industrial, scientific and medical, as well as cell

and tissue banks.

of 89CBS Isothermal

Dry Storage Freezers

Why is dry storage important?

Safety. Storage in liquid nitrogen can make vials shrink. This may

cause liquid nitrogen to seep into the vials, which on rewarming,

expand and explode as nitrogen vaporizes inside the vials. Dry

storage eliminates this possibility. Also, the Isothermal design

provides added user safety by eliminating contact with or

splashing of liquid nitrogen.

Cross Contamination. Studies have shown that viral, bacterial

and fungal pathogens can survive after suspension in liquid

nitrogen. Infected samples can cross contaminate other samples

in the same liquid nitrogen tank. Dry storage eliminates the

possibility of cross contamination.

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CBS Isothermal -196C Dry Storage Freezers

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Custom BioGenic Systems -196C Isothermal

Dry Storage Freezers Specifications

V1500-AB V3000-AB V3000EH-AB V5000-AB V5000EH-AB

LN2 Capacity (liters) 30 70 89 93 140

External Width in (mm) 26 (660) 37 (939) 37 (939) 47 (1,219) 47 (1,219)

External Depth in (mm) 37 (939) 48 (1,219) 48 (1,219) 54 (1,371) 54 (1,371)

External Height in (mm) 45 (1,143)

47.5

(1,206) 58 (1,473) 52 (1,320) 58 (1,473)

Usable Interior Height in (mm) 29 (736) 29 (736) 37 (940) 29 (736) 34 (864)

Usable Interior Diameter in (mm) 21 (534) 31 (787) 31 (787) 40 (1,016) 40 (1,016)

Weight Empty lbs(kg) 327 (148) 600 (272) 650 (295) 936 (425) 997 (453)

Weight Full lbs(kg) 383 (174) 720 (327) 808 (367) 1100 (500) 1247 (566)

Maximum 2ml vial capacity 9,100 22,100 25,500 40,300 46,500

Maximum 50ml bag capacity 434 1,120 1,280 1,936 2,208

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Know the CBS Isothermal Freezer

The unique, patented design provides the optimum environment for

preserving the long-term viability of samples with uniform temperatures

throughout the freezer in the -190°C range, including directly below the lid.

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No liquid nitrogen inside the

sample area eliminates the risk

of cross contamination and

provides added user safety by

eliminating contact with or

splashing of liquid nitrogen.

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The full internal volume is

available for storing samples

making the most efficient use

of freezer capacity. There are

no aluminum plates or

carousel walls taking up

valuable storage space.

The full width lid provides

unrestricted access to

samples.

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The outstanding

temperature uniformity and

“dry” storage condition

address requirements of

the latest European

directives for tissue

storage, in respect to

maintaining cell integrity

and preventing

contamination.


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Temperature Uniformity and Liquid Level

In conventional (non CBS) vapor phase freezers, the

temperature directly under the lid can be reduced by raising the

level of liquid Nitrogen in the bottom.

Whilst improving the temperature uniformity (gradient), this

reduces the amount of space available for storing samples in

vapor. This also makes the freezer more expensive in terms of

the number of samples stored.

In this situation, ask the customer to find out the relationship

between temperature gradient and the level of liquid Nitrogen

in the freezer, since the specified temperature may depend

heavily on the liquid Nitrogen level.

In CBS Isothermal freezers all the internal volume is available

for sample storage - free from liquid Nitrogen.

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Temperature Uniformity and Temperature Probe Location

Encourage the customer to check with potential suppliers

about the operating temperatures of their freezers and where

the temperature sensor is located.

Cold air sinks and therefore the best location for the

temperature sensor is at the top, above the samples. On the

CBS Isothermal freezers, the sensor is located directly below

the lid and above the top of the racks giving the customer

confidence that all samples are stored below the indicated

{display) temperature - see typical V-1500 mapping data

below.

If the sensor is positioned lower down in the freezer, the

displayed temperature will look good but can the customer

be sure of the temperature of samples higher up?

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Probe #1

Probe #2

Probe #3

Probe #4

Probe #5

Probe #6

An aluminum freezer rack is used for

attaching 6 temperature probes to monitor

liquid nitrogen vapor temperature.

Spacing between the probes is

approximately 4 inches (10 cm).


Fill to Fill Temperature Study

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-196°C

-194°C

-192°C

-190°C

-188°C

-186°C

-184°C

-182°C

-180°C

-178°C

Probe 1

Probe 2

Probe 3

Probe 4

Probe 5

Probe 6

END OF FILL

LEVEL AT 17"(43CM)

FILLING PROGRAM - LOW SET POINT 10" (25 CM)

HIGH SET POINT 17" (43 CM)

START OF FILL

LEVEL AT 10"(25CM)

Approximately

21 to 24 hours

fill to re-fill

10 Minute

fill period

6 Temperature probes are placed in a 13 shelf aluminum

freezer rack, equally spaced from top to bottom.


Fill to Fill Temperature Study

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This study shows that as the liquid nitrogen in your CBS Isothermal

Freezer evaporates, the internal temperature at probe #1 will rise

slightly to -182C. As the re-fill process starts, the temperature at

probe #1 will return to the normal operating temperature in the -189C

range.

The temperature of the other 5 probes increases very little, ranging

between -186C to -196C. Therefore, 85% of the freezer storage

space remains below -186C and only the top15% of the space will be

slightly warmer in the -182C range.

This study provides temperature data that confirms samples stored in

a CBS Isothermal Freezer will be well below critical temperatures.

Your CBS Isothermal Freezer is pre set to re-fill when the liquid

nitrogen level reaches a low set point of 10” (25cm) and stop filling

when the liquid nitrogen reaches the high set point of 17” (43cm).

These filling set points will provide excellent operating parameters.

However, they can be changed to suit your specific requirements.

Conclusion

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Temperature Uniformity and Thermal Conductivity

Customer: "Other freezers also offer -190°C vapor storage"

To compete with the Isothermal concept and provide greater

uniformity, other manufacturers use the thermal conductivity properties

of aluminum and install aluminum plates (frames and carousel walls)

inside the freezer. The aluminum conducts heat from the top of the

freezer, which promotes evaporation and the resulting Nitrogen vapor

rises to cool the top of the freezer.

This approach has two disadvantages;

• Firstly, the more aluminum in contact with liquid Nitrogen, the

higher the consumption will be. This is why it is important for the

customer to compare the loss rate of the actual models they are

considering.

• Secondly, the use of frames and carousels reduces the amount of

space inside the freezer for samples. In contrast, the open interior

of the CBS Isothermal freezer provides maximum storage capacity

without the restrictions of carousels etc.

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Customer: " I prefer Narrow Neck Lids as they keep the cold in"

In an effort to reduce cold air loss and liquid Nitrogen consumption,

manufacturers have introduced models with narrow neck openings. Whilst the

concept appears attractive, feedback from many customers highlights some

practical issues with this design.

Firstly, access to and location of samples is made more difficult. In contrast,

the CBS Isothermal design with Nitrogen vapor vents helps to hold the cold air

inside the freezer. This means that full-width lids can be used giving the user

unrestricted access for easier sample location.

The following temperature mapping data shows how the temperature inside a

V-1500 increases on lid opening. Even after 30 minutes the air temperature at

the top of the V-1500 remains well below the critical glass transition

temperature of water.

Secondly, if anything is dropped or becomes jammed in the carousel system of

a freezer with a narrow neck lid, it becomes almost impossible to retrieve or

repair.

Narrow Neck Lids

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An aluminum freezer rack is used for

attaching 6 temperature probes to monitor

liquid nitrogen vapor temperature.

Spacing between the probes is

approximately 4 inches (10 cm).


5 Minute Lid Open Temperature Study

Probe #1

Probe #2

Probe #3

Probe #4

Probe #5

Probe #6

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-196°C

-194°C

-192°C

-190°C

-188°C

-186°C

-184°C

-182°C

-180°C

-178°C

0:3

0:0

0

12

:00

:00

12

:30

:00

13

:00

:00

13

:30

:00

14

:00

:00

14

:30

:00

15

:00

:00

15

:30

:00

16

:00

:00

16

:30

:00

17

:00

:00

17

:06

:00

17

:12

:00

Probe 1

Probe 2

Probe 3

Probe 4

Probe 5

Probe 6

5 MINUTE LID OPEN PERIOD

COLD VAPOR FROM THE

LOWER LAYERS COOLS

THE UPPER LAYERS

TEMPERATURE

INCREASES

TEMPERATURE IS

STABILIZED TO

NORMAL OPERATING

TEMPERATURE


5 Minute Lid Open Temperature Study

6 Temperature probes are placed in a 13

shelf aluminum freezer rack, equally

spaced from top to bottom.

Freezer lid is opened.

Items are stored or retrieved.

Lid is closed.

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Lower capacity

Round lid opening may restrict access to square

racks

Narrow Neck Lids

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Liquid Nitrogen Consumption

Customer: “Other freezers use less liquid nitrogen”

The only way to measure and compare LN2 consumption of freezers is by

using the Static Evaporation Rate (also known as Normal Evaporation Rate).

This is an industry accepted method for measuring the LN2 consumption of an

empty freezer under standardized conditions. (A copy of the method is

available on request).

Many manufacturers make claims about lower LN2 consumption and running

costs but then don't support this with data in their sales material.

If LN2 consumption is an issue, we recommend that customers ask potential

suppliers for the Static Evaporation Rate (SER) for the freezers they are

considering so that like-for like comparisons can be made.

It is important that the customer asks for the SER of the specific model they are

considering, including built-in options. Some manufacturers only quote a SER

for the base model, however the actual SER could be much higher if the

customer orders optional aluminum frames or a carousel.

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Series 2301 Auto-Fill and Monitor System

2301 Front Panel DisplayFour line LCD readout

Start / Stop Fill System Status Output Data to Printer Fill Level Set Points Temperature Display – Fahrenheit / Centigrade Liquid Level – Inch / Centimeter

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Liquid Level Filling Set Points

Your CBS Isothermal Freezer is delivered with factory pre-set liquid

level filling set points.

The low set point (start fill) should be set at 10 inches (25 cm).

The high set point (stop fill) should be set at 17 inches (43 cm).

These filling set points will provide excellent operating parameters.

However, they can be changed to suit your specific requirements.

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Series 2301 Controller

2301 Features

0-5 volt outputs provide temperature & level data for connection to existing alarm/monitor system

4-20 mA outputs provide temperature & level data for connection to existing alarm/monitor system

Sequential and One-Fill-All-Fill input and output connection for filling systems

New 16 Port Connector

New two level temperature display option Temp A displays temperature at the lid, approximately 11” (27.94 cm)

Temp B displays temperature inside the storage space, approximately 20” (50.80 cm)

Additional Features Download data files to PC and be able to convert them to .csv files for use in EXCEL

Ethernet and auxiliary RS485 for future expansion

Dedicated 24VDC signal output to activate a third 24VDC valve to control the LN2 supply

Global Remote Alarm - Dry contact that switches during any alarm condition

Overflow Sensor (Optional)

Fill Timer

Data Log - Report Temp A, Temp. B and level with date and time stamp. Printed or displayed.

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Series 2301 Features / Specifications

1. Vent Thermocouple

2. Temp. A Thermocouple - Lid temp (located 11” down)

3. Temp. B Thermocouple - Temp (located 20” down)

4. Ethernet Port - For future expansion

5. RS-485 IN

6. RS-485 OUT

7. Sensor Port

8. Lid Switch

9. Fill/Vent In Progress

10. Fill Solenoid Valve Outlet

11. Vent Solenoid Valve Outlet

12. Global Remote Alarm

13. 2 Amp Fuse

14. 24 V Power Supply

15. Overflow Sensor (Optional)

16. Thermal Printer Port - For printing alarms & data

17. 16 Port Connector:

0-5V output for Temp. A, Temp. B, and Level

4-20mA outputs – for Temp. A, Temp. B, and Level

Seq. OFAF Input and Output Connection

18. Auxiliary RS 485 - For future expansion

19. USB Port

1 2 3 4 5 6 7 9 10 11 12 13

1918 17 8 16 1415

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The amount of time required to fill a freezer with liquid

nitrogen will vary with model. Listed below are the

estimated fill times for each CBS Isothermal Freezer.

V1500 – approximately 40 minutes



V5000 EH – approximately 90 minutes

Initial Filling Details

These fill times are based on a conventional 180 liter

and 230 liter supply tanks operating at 22 psi.

Fill times may vary with the use of bulk tanks and

vacuum jacketed supply lines.

cbs isothermal -190c freezer

Health & Medicine

temperature of samples

liquid nitrogen temperatures

liquid nitrogen contact

level of liquid nitrogen

liquid nitrogen level

liquid nitrogen tank

cbs isothermal freezers

temperature sensor