Catecholamines;

Basic and

Clinical Frontiers

Volume One

Proceedings of the Fourth International Catecholamine SymposiumPacific Grove, California, September 17-22, 1978

Edited by

Earl Usdin*

Psycho-pharmacology Research Branch

National Institute of Mental Health

Rockville, Maryland

Irwin J. Kopin*Laboratory of Clinical Science

National Institute ofMental Health

Bethesda, Maryland

Jack Barchas

Nancy Pritzker LaboratoryDepartment ofPsychiatryStanford University School ofMedicine

Stanford, California

*This book was edited by Drs. Usdin and Kopin in their privatecapacities. No official support or endorsement by the NIMH is

intended or should be inferred.

universitatsbiblioyhekhannover

TECHNISCHEINFQRMATIONSBBLIOTHEK

Pergamon Press

NEW YORK • OXFORD • TORONTO • SYDNEY • FRANKFURT • PARIS

TABLE OF CONTENTS

Volume I: Catecholamine Metabolism, Receptors, Neuropharmacology

Introduction (Earl Usdin3 Irwin J. Kopin, Jack D. Barchaa).

xxi

Participants xxviii

Abbreviations xlix

Catecholamine Research: A Paradigm for the Relationship Between

Basic Investigations and Clinical Applications in Psychiatry [GeAaldL. Klttman) 1

The Impact of Catecholamine Research on Medical Science and

Practice [knv-id CemZiion) 4

Section A: Catecholamine Synthesis and Catabolism 21

A Genetic Control of the Number of Central Dopamine Neurons in

Relationship to Brain Organization, Drug Responses, and Behavior

[Vonald J. Rtit>, WanxLet Baktn., J. Ste.phe.n Tlnk and Tong H. Joh). 23

Regulation of Tyrosine Hydroxylase [Tohkihalux Nagat&u,] ... 34

Activation and Phosphorylation of Tyrosine Hydroxylase by cAMP

[Jack C. Waymint, John W. Haycock, John A. HeJU.ge.n-l and Mike V.

Bwwnlng) 40

Direct Phosphorylation of Tyrosine Hydroxylase by Cyclic

AMP-Dependent Protein Kinase: Mechanism of Enzyme Activation

(Tong H. Joh, Vong H. ?an.k and Vonald J. Rexi) 43

Tyrosine Hydroxylase from Brain: Phosphorylation of the

Enzyme by Cyclic AMP-Dependent Protein Kinase [Joachim V. Rat&t,khthuA. M. Edelman, MtcheJUL A. lazax, and Jack V. BaAckai,). ... 46

The Activation of Tyrosine Hydroxylase in NoradrenergicNeurons During Acute Nerve Stimulation (W. WeZneA, M. Poiiviata.,E. VhtyeA. and F.-L. Lee.) 49

Serotoninergic Control of Tyrosine Hydroxylase (TH) and

Dopamlne-B-Hydroxylase (DBH) in the Rat Locus Coeruleus (LC)(J.F. Vujol, A. yicV.ae.-VtQixtim.ce., F. CueMpl, P. Ktant, B. Rtnaud,L. LtgeA and M. Buda) 52

The Pharmacology of Striatal Pterins and the Regulation of

Dopaminergic Function [WJJL&on P. Baltand, Jo&tpk B. Vtltin andArnold J. Mandell) 55

The Role of Neural Activity in the Regulation of the Bio¬

chemistry of Neurons and Their Target Cells {ULckaAd E. Z-igmond,Alcmint ChaZazonLtL/i and Ckau.nc.ty W. Bowtu) 58

Role of Protein Kinase Translocation in the Induction of

Tyrosine-3-Monooxygenase (TF) in Adrenal Chromaffin Cell Cultures

(K. KumakuAa, A. GaldottL and E. Co&ta.) 61

Relation Between Brain Tyrosine and Catecholamine Synthesis(R.J. WiMtman, M.C. ScalZy, C.J. Glbion and F. Htiti) 64

V

vi FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM

Effect of Bivalent Cations on the Uptake of L-Tyrosine by

Synaptosomes (J. B/iulnveZi and P. Holtman) 67

Isolated Bovine Adrenal Medullary Cells: Studies on Regulation

of Catecholamine Synthesis and Release [Motoo Oka, UlnoKu, JiOisOki

and Nobu.yu.ki ya.na.gi.hMa.) 70

Central Neurogenic Regulation of Adrenal Tyrosine Hydroxylase

(S. GanthitJi, J.-P. GagnoA and T.L. SauAkti) 73

Rat Pheochromocytoma Tyrosine Hydroxylase and Dopamine B

Hydroxylase: Purification and Characterization (K.A. MaA.kty,J.C. T-ong, M. Golditeln and L. Sktnkman) 76

Amphetamine-Induced Inhibition of Synaptosomal Dopamine

Synthesis Activation |Robe/i£ L. Vathlck) 79

The Involvement of Calcium In the Amphetamine-Induced

Stimulation of Dopamine Synthesis in Striatal Slices {Nofunan J.

Uittbky and S. Robt/ut SnodgAcai) 82

Regulation of Dopamine Synthesis within Striatal Synaptosomes

{Gltgoty KapatoA and ULchaet ligmond) 85

The Regulation of Tyrosine 3-Monooxygenase Activity in

Pheochromocytoma Cells (RobeAi i-. VeAlman) 88

Short- and Long-Term Control of Tyrosine Hydroxylase Activity

in Nigrostriatal Dopamine Neurons: Multiple Neural Mechanisms

[KaAtn Gait) 91

Tyrosine Hydroxylase: A Substrate for Cyclic AMP-DependentProtein Kinase In Vitro (P.R. Vultttt, T.A. Langan and W. WtineA). 94

The Effect of Carbachol on the Induction of Tyrosine Hydroxy¬lase Elicited by Compounds which Raise Cyclic AMP Levels in

Neuroblastoma Cells (A.W. Tank, H. Poi-tvixiXa and W. WtineA). ... 97

Similarities Between the In Vivo Activation of Adrenal Tyrosine

Hydroxylase and the _In Vitro Activation of the Enzyme by an Adenosine

3',5'-Monophosphate Dependent Protein Phosphorylating System

(J. Maiitnano and W. Welnen.) 100

Modulation of Tyrosine Hydroxylase Activity by Phospholipids:The Effects of Phosphatidylinositol (Tom Lloyd) 103

The Role of Cyclic Nucleotides in the In Vivo Synthesis of

Catecholamines in Rat Brain [Wol&gang KthA and GeAlindt Vtbuj,) . . 106

Significance of Tyrosine Hydroxylase Activation in the Re¬

gulation of Dopamine Synthesis (B. Zivkovic) 109

Regulation of Hypothalamic Beta-Adrenergic Function by

Phosphatidylserine [S. Ttolato, G. Savoi.nl and G. Toi&ano) .... 112

Effect of Nerve Growth Factor on General Growth and Differenti¬

ation of Sympathetic Neurons: Induction of Ornithine Decarboxylaseand Tyrosine Hydroxylase as Respective Examples [liitit Oittn, hitiotihl

Hata.na.ka and Ham Thotntn) 115

Tyrosine Hydroxylase Activity in the Mouse Superior Cervical

Ganglion Infected with Herpes Simplex Virus (HCcka/ld ill. VhJjct, TongH. Joh and Donald J. ReX6) 118

Retinal Tyrosine Hydroxylase: Stimulation In Vivo by Light and

In Vitro by cAMP-Dependent Protein Phosphorylation Conditions (P. M.

luvont and hl.H. NtU) 121

The Metabolism and Function of Monoamines in Iron-

Deficient Rats (M.B.H. Voudim and A.R. G/ittnt) 124

Round Table on: Is There DOPA Decarboxylase in Human Brain?

(W. Sack*, W.H. Vogel, T. HagaUu., Ktnntth G. Lloyd and M. SandleA) 127

FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM vii

Enzyme-Activated Irreversible Inhibition of Aromatic L-Amino

Acid Decarboxylase (AADC) by o-Difluoromethyl DOPA (DFMD, RMI 78.801)and a-Monofluoromethyl DOPA (MFMD, RMI 71.963) [flichatl G.

Pal&ityman, ULchtl J. Jung, Chanlti Vanzin, GilleM RibeAtaa-Gayon,PhiXxppt Bty, Uoniqut Z/uaika and KlbenX SjoeAdsma) 132

Isolation and Preliminary Amino Acid Analyses of Dopamine 8-

Hydroxylase (DgH) From Purified Large Dense Cored NoradrenergicVesicles (LDV) [ilen-fi&an Vang, Hilton S. Ga&poAU and RichaAd L.KleJjn) 135

Reduced Rate of Synthesis of Dopamine-B-Hydroxylase in LocusCoeruleus During Axonal Regeneration (R.A. "R.OA&, T.H. Joh andV.J. ReXi] 138

Comparisons of Antigenically Pure Dopamine B-Hydroxylase(DBH) From Bovine Large Dense Cored Noradrenergic Vesicles (LDV)and Adrenomedullary Chromaffin Granules (AMG) [RichaAd L. Kltin,Dltn-Hiu-n Yang, and Hilton S. GaipoAii) 141

Isotope Effects in the Hydroxylation of Dopamine by Dopamine¬

s-Hydroxylase (DBH) (T. Koto, T. Nagat&u, V. Hashimoto, H. Hiyazaki,M. LtvUZ and J.M. PeAel) ...'... 144

Human Serum Dopamine-B-Hydroxylase Biochemical Genetics [JoelVunntttt and RichaAd Wtinshilboum) 147

The Role of Dopamine (DA) in Noradrenaline (NA) ^Biosynthesisin Hypothalamic Neurons [Uuzianna HuAzti, Geza Szilagyi and End/itKa&ztAtineA) 150

The Role of Dopamine (DA) in Noradrenaline (NA) ZiU-Zianna.

Hwizti, Gtza Szilagyi and End/it Ka6zt/izineA) 150

N,N-Dimethyl-N'-lH-l,2,4-Triazol-3-yL-Thiourea, A Potent

Dopamine-B-Hydroxylase Inhibitor (I/. ClaaA&tn, T/l.A.C. Bo&ckman,F.C. Hilltn and W.J. Vat&txa) 153

Catecholamine Turnover lima in Selected Parts of the Rat

Brain During Hypobaric Hypoxia (M. VKioux-Guyonntau., E. QJteteX.,C. Jacquot, J.R. Rapin and y. Cohtn) 156

Phenylethanolamine-N-Methyltransferase and Epinephrine in

the Brain (M. Golditeln, A. SauteA, J.V. Lew, y. Baba, J. Engtl,K. Fuxe and T. HnkleZt) 159

Regulation of Phenylethanolamine N-Methyltransferase Synthesis

and Degradation (Retold V. CiaAanello) 162

Regulation of Phenylethanolamine-N-Methyltransferase in Cultured

Explants of Rat Adrenal Medulla (W.J. BuAkt, J.W. Vavlb, V.J. RtU

and T.H. Joh) 168

Adrenaline Synthesizing Enzyme Distribution in Human Brain

(A/. Kopp, L. VtnoKoy, B. Rtnaud, J.F. Pujol, N. Gay and M. Toimaii) 171

Epinephrine Forming Enzyme in Human Brain [Elizabeth Endtlyi,Roland V. CiaAanello and Jack V. BaAchai] 174

Phenylethanolamine N-Methyltransferase From Rat Brain and

Adrenal Medulla of Rat [PtttA H. Vu.) 177

The Effects of Hypophysectomy on the Kinetics and Regulation ofPNMT in the Brain and Adrenal Glands of Rats [(Hitla Phyall andDlalteA Lovtnbtug) 180

Role of Stress in the Regulation of Central EpinephrineSynthesis (8.8. TuAntA, R.J. Katz and B.J. CaXAoll) 183

Lowering of Brain Epinephrine by Inhibition of NorepinephrineN-Methyltransferase in Rats [Ray to. PutltA, Ktnntth III. PeAty, SudanK. Hemlck and Buyan B. Uolloy) 186

Vi 1 i FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM

Some New Inhibitors of Epinephrine Biosynthesis. Importance of

the Aromatic Ring in Adrenergic Amines [GaAy L. Gluxntmald, WiZtiam

C. Vinctk, Vuight P. Vault, and Ronald T. BoAchandt) 189

Expression of Catecholamine-Synthesizing Enzymes in Human

Neuroblastoma Lines with Unique Chromosomal Abnormalities [Junt L.

BitdleA, BaAbaAa A. SptngleA and RobeAt A. Ron) 192

On the Role of Hydroxylic and N-Methyl Groups in the Interaction

of Phenylethylamines with Monoamine Oxidase Types A and B (E.A. ZtlltAand K.L. A/ioKa) 195

The Topography of Mitochondrial MAOs [S. Dlelii and R. UcCauZty) 198

Dopamine Oxidation and its Inhibition by (-) Deprenyl in Human

Brain and Tissues [Vlvettt GLovtA, J.V. EJUviOAth and M. Sandlejtf. . 201

Substrate Specificity and Inhibitor Characteristics of Brain

Monoamine Oxidase at the Mitochondrial Level (F-tanceA M. Acktt and

Sabit Gabay) 204

Benzylamine Oxidase in Human and Rat Tissues [Rachtl Ltwin&ohn,ViveXtt GloveA, K.-H. Bote and M. SandltA) 207

Catecholamine Metabolism and Drug Effects in Cultured Mammalian

Cells: Analysis by HPLC [PettA A. CAooki, XandAa 0. Bltakt£leZd andJamti K. Cowand) 210

Conjugates of 3-Methoxy-4-Hydroxyphenylethylene Glycol in Man

(A.R. BoobiA, S. MuAAay, H. Jonti, J.L. Rtid and V.S. Vavlti) ... 213

Catechol-O-Methyltransferase Biochemical Genetics [RichaAdWelnihilboum) 216

Immunocytochemical Localization of Catechol-O-Methyltransferasein Cardiovascular Tissues [tiichatl C. Lowt and CyAuA R. CAtveZing). 219

Molecular Aspects of Monoamine Oxidase Active Site (M.8.H.yotidZm and J.I. SaZach) 222

Effect of a Catechol-O-Methyl Transferase Inhibitor, U-5021,With Levodopa Administration [Stanley Fahn, RichaAd Comi, StaaAt R.SnideA and A.L.N. PtiaAad) 225

Section B: Catecholamine Release, Storage and Uptake

Dendritic Release of Dopamine: Its Role in the Control of Nigro-strlatal Dopaminergic Neurones (Jacques Gloutiniki and AndAi. CheAamy) 231

Current Issues in the Study of Norepinephrine Secretion From

Noradrenergic Nerves [LtnnaAt StjaAnt) 240

The Mechanism of Neuronal Release of Adrenergic Transmittersby Low Sodium and Hypo-Osmotic Shock [E. \kuAcholl and H. RJJtztl). .

244

Synthesis and Release of Cyclic GMP by Nerve Endings in the Rat

Pineal Gland and Neurohypophysis [UaAtin Zatz and RobeAt F. O'Veja). 2hl

Isolation and Characterization of the Methyl Acceptor Proteinfrom Adrenal Chromaffin Granules [Ronald T. BoltchoAdt, JuZit Olttn,Ltt Eidtn, RichaAd L. Schowtn and ChanZeA 0. Rutltdgt) 250

Characteristics of Presynaptic Inhibition of NorepinephrineRelease by Adenosine and Related Analogs [David M. Paton) 253

Adenosine as Modulator of Adrenergic Neuroeffector Trans¬mission [PeA HzdqviAt and BtAtiZ 8. FKtdhalm) 256

In Vitro Studies on Presynaptic Alpha-Receptor MediatedModulation of Transmitter Release from Central NoradrenergicNeurons [Axit H. WxZdtA, Ctt6 V.J. dt Langtn, Johan WemeA and

Kty VZ&muktb) 259

FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM IX

Vesicle Populations and Exocytosis in Noradrenergic Terminals

of Human Veins (Aia. ThuAtion-Kleln, RichaAd i, Klein and Ltnnaxt L.

Stj'aAnt) , 262

Effect of Dopamine on the Nerve-Stimulation Mediated Release

of Norepinephrine (NE) and Dopamine B-Hydroxylase (DBH) .from the

Isolated Perfused Cat Spleen (W.R. Vlxon, W. Mo&imann and N.

itltlntA) ,..,,.,,.265

On the Mechanism of Neuronal Efflux of Axoplasmic 3H-(-)Noradrenaline [KaAl-Htlnz Ghat^t and Guntex F«oAi) 268

An Adrenaline-Mediated Positive Feedback Loop in SympatheticTransmission [HtnAyk Majemki, MaAlan McCuZloch, iiCchatl Rand and

Vavid Stony) , , . ,271

Possible Involvement of Cyclic AMP in Facilitation of Nor¬

epinephrine Release Mediated by Presynaptic Beta-Adrenoceptors in

the Rat Pineal Gland (M.L. Vubocovich, F. Ptlayo and S,Z. LangtA). 274

The Mechanisms of PGE, Alpha Adrenergic and Beta AdrenergicRegulation of Catecholamine Release: Evidence for Different Modes

of Action [Vthada. Gutman),

277

Regulation of Catecholamine Release: The Cholinergic Mechanism

[Ovt A. MtdtAgaaAd) , ,280

Excitation-Secretion Coupling: Increased Protein Carboxy-

methylation During Neurogenic Stimulation of the Adrenal Medulla

(0. HumbeAto VIvzaoa and Emanu.eZ J. V^tcbeAto, J4..J, ,283

Control of Dopamine Release from Hypothalamic Neurons

[Fiuxncint Vtnlztau. and FeAnand Labnlt) ..,.,.286

Effects of Verapamil and Presynaptic Modulators on Calcium-

Induced Noradrenaline Release (M. Gothext, P. NaW/tolh and I.-M. Pohl) 289

Models of Regulation of Norepinephrine Secretion by Pre¬

junctional Receptors and by Facilitation: Role of Calcium and

Cyclic Nucleotides [LtnnaAt Sti'd/mt, PtteAli AlbextA and Tama&

BaAt&al) 292

Effects of g-Adrenoceptor Agonists and Antagonists on Neuronal

Transmitter Release in the Rat Portal Vein [CahZ VahZ'6{, Btngt Ljangand Btngt Ablad) ,

298

Studies on the Release of Propranolol from Adrenergic Neurons

[J.G. Wtbb, T. Waltz, H.B. VanleZl and T.E. Gaiinty) 301

Evidence for Carrier Mediated Efflux of Norepinephrine Displaced

by Amphetamine [ChaAltS 0. Rutltdgz a,nd Sheila. VoltmeA) 304

Plasma Membrane Noradrenaline Transport: a Rotary LiquidPore Model [SeAglo Sdnchzz-A/una&i a,nd FeAna,ndo QKAtgo) .

307

Functional Differentiation within a Single Type of Adrenal

Chromaffin Cell [S. Kobayashl, T. HiAaw, N. Qzakt, T. Fajita. and

R.E. Cou.pla.nd) 310

SGC Cell: The Third Type of Adrenal Chromaffin Cell (R.E.

Coapland, S. Kobayaihl, /. SeAlzam and T. Fu-jlta.) 313

Ascorbate Uptake and Metabolism by Adrenal Medullary Chromaffin

Granules (J.G. TiAAeZZ and E.D1. UleAthtad) 316

Identification of Anion Transport Sites in Chromaffin Granules

and Their Involvement in an Osmotic Mechanism for Epinephrine Release

[ChAiitophex J. Pazolti, CanZ E. Owxtz, and HaAvty B. PolZaxd). . . 319

X FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM

Chromaffin Granule as a Model System for the Study of Exocytosis

[Fatoshi Izuml, Taktshi Miyashlta, Taktshi Hashimoto and Aklklko Wada)322

Structural Changes in Chromaffin Granule Membranes During Exo¬

cytosis [Sttphtn J. HomIs and Vuncan H. Haynts) 325

Evidence in Support of a Chemiosmotic Mechanism for Exocytosis

from Platelets, Parathyroid and Chromaffin Cells [HaAvty B. Pollaxd,

ChAistopheX J. Pazolts and Caxl E. CAtutz) 328

Use of Dopamine g-Hydroxylasa in the Study of Vesicle Dynamics

(R.A. Rush, T.J. MllloA, l.W. Chubb and L.B. Ge^m) 331

Quantitative Aspects of Dopamine-g-Hydroxylase as a Marker for

Exocytosis of Norepinephrine and Epinephrine [GuldboKg SeAck-HanS&tn,Gtitta Bolstad and Kaxtn 8. HtlZt) 334

Characterization of the Proteins of the Chromaffin Granule

Membrane (E.W. Utsthtad, W. Fojiguson, K.L. Bnayman and H. L. Gibson). 337

Identification, Isolation and Partial Characterization of Two

Types of NA Storage Vesicles in Dog Sympathetic Ganglia [W.P. Vt PotteX,F.H. Vt Smet, E.J. Van de. Vtldz and J. Quatackex) 340

Regulation of Adenylate Cyclase in Chromaffin Granule Membranes

[Oitn Zlndex and HaAvty 8. Pollaxd) 343

Synexin: An Adrenal Medullary Protein that May be an Intra¬

cellular Receptor for Ca in the Process of Exocytosis [CaAl E.

CAtutz, Chnlstxiphex J. Pazolts, and HaAvty B. Pollaxd) 346

Small Noradrenergic Vesicles from Vas Deferens of Castrated

Rats: A Comparison with Large Noradrenergic Vesicles [GabAlel Fhltd,

Hugo Lagexciantz xnd Tomas Hokitlt) 349

Fluorescent Probe Studies of Isolated Chromaffin Cell Membranes

(F. SchntemelSi,, V.V. Naqulna, K. Ro&tnhtck and A.S. Sckneldex). . . 352

Isolation of Plasma Membrane from Adrenal Chromaffin Cells

[V.J. GAttn and E.W. Wtsthtad) 355

Actin and Myosin in Chromaffin Cells: Characterization and

Roles in Cell Function (J.M. TAi^aXO and R. Ltt) 358

Catechol-Binding Protein in Rat Liver: Solubilization and

Some Properties [J.H. Tong and A. V'lonlo) 361

Uptake, Metabolism and Release of^Catecholamines in the Lung

[CnystaZ A. Ltsllt, AZtksandeA A. Matht, Edmund Y. Tong, LadlsluvVoliceA and RichaAd Sohn) 364

Photoaffinity Labeling of Adrenergic Receptors in Neuronal

Membranes [Kim AZlyn HeldtnAtlch and Edith V. Htndlty) 367

A Phospholipid-Protein Model of Neurotransmitter ReceptorActivity [RobzXt 0. Fxltdtl) 370

Hydroxylated Metabolites of Tricyclic Antidepressants: In¬

hibition of Amine Uptake and Other Pre-Clinical Studies (W.Z.PotteA, tf.M. Colli, A. Manian and F.K. Goodwin) 373

Use and Limitations of Blood Platelets as Models for Mono-

aminergic Neurons (A. PZtticheA, A. SaneA, A. Laubschex and M. G/ia&) .376

Effect of Arrhythmogenic Doses of Deslanoside on the Uptakeof Monoamines in Cardiac and Brain Tissue, and in Platelets (C.J.HeZkt, K.J. KeZloA and R.A. GUUs) 379

Electron Microscopic Localization of Tetrahydropapaveroline inthe Rat Caudate Nucleus [L.Y. Koda, W.T. Shiex, G.F. Koob and F.E.

Bloom)382

FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM XI

Section C: Catecholamine Receptors

Presynaptic Receptors and the Regulation of Transmitter Release

in the Peripheral and Central Nervous System: Physiological and

Pharmacological Significance [S.Z. Langex) 387

g-Adrenergic Stimulation of Protein Carboxymethylation and

Amylase Secretion in Rat Parotid [llloHAtn J. StAlttmatteA, Claude

Gagnon and Julius Ay.tln.od) 399

Changes in Vascular Adrenoceptor Characteristics But Not Other

Structural and Functional Parameters Correlate with Embryological

Origin of Cerebral Blood Vessels [John A. Btvan and RostmaAy V. Btvan) 402

A Reappraisal in Guinea-Pigs of Drugs Proposed to be Selective

g2-Adrenoceptor Antagonists [Janet C. WanStaZl, Stella R. O'VonneZl

and Kaxin Walduck) 405

Interaction Between g-Adrenoreceptor Stimulants and Certain

Phosphodiesterase Inhibitors on Smooth Muscle (J. Q^eAmeleA, H.J.

GxitsseZ and F.G. van dtn Bhlnk) 408

a-Adrenergic Receptors in Rat Aortic Smooth Muscle: Evidence

for Two Cooperatively Interacting Populations [JamtS P. Noon, PtteA

J. Rict and Ross J. Baldtssaxini) 411

Desensitization of Guinea Pig Vas Deferens in Relation to Alpha-

Adrenoreceptor Ligand Binding [B.H. UaAkS and M.I. Hoick) 414

Interaction of Dopamine Agonists With Peripheral a-Adrenoceptors

[Alan Gibson and HoAttza Samini) 417

Actions of Catecholamines and Cocaine at Inhibitory Adrenore-

ceptors in Rat Vas Deferens [Joctlyn N. Pznnz{atkeA, Guiynntth M.

Handbexg, Sttphtn V. Shiplty and Vavld A. Tayloft.) 420

Receptor-Effector Coupling in Cardiac Muscle (Joan HeZleA BAOltin

and Sttvtn E. Uayex) 423

Pre-Synaptic Alpha-Adrenergic Receptors of Cerebral Arteries

[Sat PlptA VuckltS) 426

The Essential Role of Calcium in the Response of the Smooth

Muscle Cell Membrane to Catecholamines [Tadao Tomita, Sadahalm. liiunt

and Edith B'dZbxing) 429

Contribution of Cyclic AMP in the Delayed Effect of Noradren¬

aline on Chronotropism of Sinoatrial Node with Simultaneous Ap¬

plication of Noradrenaline and Acetylcholine [MotakatSU. FujilM/ia,Takashi Tanlguchl and Hixoyoshi Hidaka) 432

Subheterogeneity of g-Adrenoceptors in the Pulmonary and the

Cardiac System of the Guinea Pig (J. Zaagsma, R. 0udho{, P.J.CM. van

dex Heljdtn and J.F. Plantft) 435

Presynaptic Aminergic Receptors Modulate a Rate of Change of

Transmitter Release [Susan A. Ntwlin, WexneA T. Schlap{eA and Samuel

H. BaAondts) 438

Regulation of Alpha-Adrenergic Response by Estrogen (J.M.

RobeAts, R.V. Goldiltn, A. Goldiitn and P.A. InseZ) 441

Selective a-Autoreceptor Agonists [NiZs-EKik Andtn) 444

Structure Activity Relationships of Pre- and Post-Synaptic

Dopamine Receptors Mediating Vasodilation [Lton I. GoldbeAg, Jal

V. Kohti, Jay J. Listlnsky and John V. McVzxmtd 447

Evidence for Central Adrenergic Regulation of Cerebral

Vascular Permeability and Blood Flow (B.K. HaAtman, J..W. Swanson,

M.E. Ralchlt, H.B. Clank and S.H. Pxukohn 450

X i i FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM

Pharmacological Influences on Pre- and Postsynaptic a-Adren-

ergic Receptors [D!ol{gang Hot{,kt and list StxeZZex) 453

Inhibition of Renin Release by the Sympathetic Nervous System

Via a-Adrenergic Receptors (VltttX KZauS tttyex) 456

Are Heart Rate and Contractility Controlled by Different

g-Adrenoceptors? [AndeXS HtdbeXg and Enax CanZsson) 459

Relationship Between g-Adrenergic Receptor and Specific

Myocardial Protein Synthesis (N.E. Naitchi, W.W. TuZchin, J. rltldman,

R.V. CiaAaneZlo, J. Axtlxod) 462

The Trophic Effect of the Sympathetic Neuron on the Artery

Wall in Growing Rabbits [R.V. Btvan and H. TsuAu) 465

Catecholamine Receptors in the Central Nervous System: Multiple

Subtypes [K.P. Hinneman and P.B. Mollno{&) 468

The Chemistry and Pharmacology of Catecholamines and Other

Low Molecular Weight Ligands Conjugated to Carriers [K.L. Utlmon,

U.S. VeAlandzn, L. Kxasny, M. Goodman, N. Kaplan, W. Castagnoliand P. InseZ)

474

Regulation of g-Adrenergic Receptors in Human Astrocytoma

Cells (T.K. Haxdtn, S.J. Fasten and J.P. Pexklns) 477

Tachyphylaxis to Catecholamines in Cultured BHK Cells (G.A.

Robinson, S.J. Stxada, W.J. Thompson, I. ttohlskima and B.

Coutunz-MuxilZo480

Refractoriness to g-Adrenergic Agonists in S49 Cells (P.A.

InseZ, M. Shtax, M. Sanda, K.L. Utlmon, J.W. Ptabody, LM.

Stoolman and M.S. Ktnntdy) 483

Inhibition of Magnesium Transport by g-Adrenergic Agonists

is Not Mediated by Cyclic AMP [Hichatl E. Magulnt, Sttphanlt J.

B-ixd and JoJStph J. Endos) 486

Control of g-Adrenergic Receptors in Hela Cells [John F.

TalZman, Richaxd C. HtnntbeAny, Fusao Hixata and Julius AxeZond). .489

Receptor-Mediated Cyclic GMP Formation by an Adrenergic Clone

(NIE-115) of Mouse Neuroblastoma [John E. Taylon and Elliott

Richtlson) 492

Catecholamine-Induced Desensitization of the g-Receptor-Linked Adenylate Cyclase [John P. PtnklnS, Ylng-iu. Su and T.

Kendall Handtn) 495

Beta Adrenergic and Dopamine (DA) Receptors in Brain

Capillaries [Gtnt C. PalmeA) 498

Phospholipid Methylation, Membrane Fluidity and Coupling of

the g-Adrenergic Receptor [FuSao HiXata, Wanntn J. StnittmatteX

and Julius AxeZxad) 501

The Turkey Erythrocyte: A Catecholamine-Responsive Cell in

Which to Study the Possible Role of Protein Phosphorylation in

the Control of Cation Transport [S.L. AlptA, H.C. Pal&nty, K.G.

Beam and P. Gnttngand) 505

3H-Catecholamine Binding to CNS Adrenergic Receptors: Guanine

Nucleotide and Divalent Cation Interactions [David C. U'PXichaxd

and Solomon H. SnydeA) 508

Guanyl Nucleotides are Endogenous Components of the Brain

Which Couple Catecholamine Receptors to Adenylate Cyclase [T.C.Chtn, I.E. Cott and J.W. Ktbabtan) 511

FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM Xiii

Spiroisobenzofuran Piperidine Derivatives. Interaction With

Pre- and Post-Synaptic Dopaminergic Sites [S.K. Puxl, C.R. Mntlont,W. Petko and V.B. Ellis) 514

Pharmacological Characterization of the Catecholaminergic Re¬

ceptors Regulating cAMP Accumulation in Rat Striatum [H.H. Sanaa,J.A. HeZtztl, P.E. Sttltn and J.J. Foley) 517

Adrenergic Receptors and Adenylate Cyclase in Membrane Pre¬

parations From the Central Nervous System (J.W. Valy and C.R.

Pantlngton), 520

Characterization, Adaptation and Regulatory Changes of the

Norepinephrine (NE) Receptor Coupled Adenylate Cyclase System in

Limbic Forebrain Structures [Philip L. Uoblty, Radhakant Mishnaand Fnidolln SuZsex) 523

In Vivo Cyclic Nucleotide Content: Effects of DopaminergicAgonists and Antagonists (G.R. BnttSt, R.B. Mailman, R.A. HutlleA,and V.B.A. Lundbtng) 526

Modulation of Cyclic Nucleotide Metabolism by AntipsychoticsThrough a Non-Dopamine Receptor [Benjamin ilitlss, RobeAt M. Levinand Loulst H. Gntznbtng) 529

Catecholamine Sensitive ATPases in the Central Nervous System(I.K. Ho and V. Vtsalah) 532

Catecholamine Receptor Binding in Brain [Solomon H. SnydeX,Vavld C. U'Pnlchand and Ian CAttst) 535

Characterization of the Efficacy of B-Adrenergic Receptor

Agonists in Terms of the Steady State Model for the Activation of

Adenylate Cyclase [John P. PeXkins, T. KtndalZ Haxdtn and Ying-iu. Su) 542

The Beta-Adrenergic Receptor of .Frog Erythrocyte: Purification

by Biospecific Affinity Chromatography (Mate. G. Canon, VogambalSninlvasan, Josti Pitha and RobeAt J. Lt{kouxitz) 547

Positive Homotropic Cooperativity of Presynaptic [3H]-Dihydro-ergocryptine Binding in Rat Heart Membranes [PaScaZt Gulcktnty,Rlcando P. Ganay, CZaixt Ltvy-Matichal and Phlllippz Mtytn) 550

Nature of Dopamine Receptors in the Striatum and Nucleus

Accumbens (A. HltAl, W.J. fdelneA and H.L. Klawans) 553

Different Neuroleptic Receptors in Various Rat Brain Areas

[Jostt E. Ltystn) 556

In Vitro Desensitization of B-Adrenergic Receptors in Rat

Cortex (M.P. Vibntn and P.B. MolinoU) 559

3H-Spiroperidol Binding in the Rat Striatum: Evidence for

the Presence of Two Binding Sites [Michael S. Bnllty and SalomonZ. Langex) 562

3 *}

H-Domperidone and JH-Pimozide: More Specific Ligands for

Dopamine Receptors Studied in Vitro and In Vivo [Michel BaudAy,Maxit-Pascalt Mantnts and Jtan-Chanlts Schwantz) 565

Enantioselectivity in the Binding of (+)- and (-)-2-Amino-

6,7-Dihydroxy-l,2,3,4-Tetrahydronaphthalene and Related Agoniststo Dopamine Receptors [John V. McVtnmtd, Hanold S. Fnteman and

Robtnt M. FeAAls) 568

Biochemical Evidence for 'Pre-' and 'Postsynaptic' Alpha

Adrenoceptors in Rat Brain [Ptttn J. M-ia.cn, Jtan-Pltnnt VauSSt and

PhiZippt Utyen) 571

Xiv FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM

3Comparison of H-Dihydroalprenolol Binding to Putative B]_-

and g2-Adrenergic Receptors Sites in the CNS of Several Mammalian

Species [Lauxa Sunn and Vavid B. Byland) 574

Decreased CDR Release From Striatal Membranes of Chronically

Haloperldol Treated Rats [MaAgaAtt E. Gntgy) 577

Regulation of g-Adrenergic Receptors in Rat Cerebral Cortex

[P.B. MolinoU, B.8. Wol&t, K.P. Mlnnzman and M.V. Vlbntn) 580

Catecholamine Receptor Stabilization: A Possible Mode of

Lithium's Anti-Manic Action [Candact B. PeAt, Agu PeAt, Jack E.

Rosenblatt, John F. Tollman and WiZliam E. Bunnty, Jn.) 583

g-Adrenoceptor Sensitivity Following Psychotropic Drug Treat¬

ment [S.K. Chonda, V.K. Shaxma and S.P. Bantnjtt) 586

Central Effects of Beta-Adrenoceptor Antagonists [Ollt AZmgntn,

Jdngzn Engtl and Jon Jonason) 589

Mesolimbic Norepinephrine and Changes in Behavioral Responsive¬

ness to Apomorphine in Rats (A.R. Cools, P.AM, van Vongtn and P.

van WtlZ) 592

Subsensitivity of Striatal Dopamine Target Cells After Re¬

peated Treatment With Apomorphine Dipivaloyl Ester (B. Station,M. Bnilty and P. Wontns). . 595

Autoradiographic Localization of Dopamine and Neuroleptic

Receptors in the Rat Brain Using 3H-Spiperone [L. ChaXltS MuXXln,Nikolai KZemm and Michael J. Kalian) 598

Multiple Dopamine Receptors [Ian Cnttst and Solomon H. SnydtA). 601

Two Populations of DA Receptors in Rat Striatum and Their

Relation to the Effect of Neuroleptics on DA Metabolism (P.F. Spano,S. Govoni, E. Sttianlnl and M. Tnabucchl) 604

Section D: Electrophysiology

Central Noradrenergic Neurons: Structure-Function Considerations

[Floyd E. Bloom) 609

Central Noradrenergic Neurons: Interaction of Autoregulatory

Mechanisms with Extrinsic Influences [G.K. Aghajanlan and J.M.

CtdaAbaum) 619

Projections of the Locus Coeruleus in the Rat [RobeAt Y.

Uoont) 622

Activity of Norepinephrine-Containing Locus Coeruleus Neurons in

the Unanesthetized Squirrel Monkey [Sttphtn L. Foott and Floyd E.

Btoom) 625

Interactions Between Antipsychotic Drugs and Central Nor¬

adrenergic Pathways [RobeAt Fnzzdman and Bonny J. Holltn) 628

Spontaneous Activity of Striatum Cells and Their Sensitivity

to Apomorphine in Dopamine-Lesioned Rats [Wol&nam SchuZtz and Unban

UngtAstzdt) 631

Effects of Chronic Haloperidol Treatment on Spontaneous Activityin the Caudate Nucleus (l.R. Skinboll and B.S. Bunnty) 634

Effects of Dopamine Agonists on Dopaminergic Unit Activity

(J.R. Waltexs, M.V. Boning and J.M. Lako&kl) 637

Action of Antidepressants on the Spontaneous Firing of Locus

Coeruleus and Dorsal Raphe Neurons of the Rat (A.E. VAtSSt and J.

Scuvtt-Moneaui 640

FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM XV

Locus Coeruleus Neurons in Freely Moving Rats Exhibit Pronounced

Alterations of Discharge Rate During Sensory Stimulation and Stagesof the Sleep-Wake Cycle (G. JontS, M. Stgal, S.L. Foott and F. Bloom) 643

Electrophysiological Evidence for Dopaminergic Agonist and

Antagonist Effects of LSD [Bonny L. Jacobs and Gnzg R. Chnlstoph). .646

Role of Catecholamines in Bulbospinal Sympathetic Pathways[Vonald N. Fnanz) 649

Dual Control of CAs on Electrical Oscillabllity J Stabilityof Identifiable Neurons [Nicolas Chalozonltls) 652

Section E: Effects of Drugs

Neurotensin-Neuroleptic Similarities: An Example of Peptide-Catecholamine Interactions (M.A. Lipton, G.N. Envin, 1.5. Bixktmo,C.B. Nemtnoii and A.J. Pnangt, Jn.) 657

Lithium Decreases and Cocaine Increases the Bilateral Asymmetryof Serotonin in Mesostriatal and Mesolimbic Systems Associated with

Changes in the Kinetic Properties of Tryptophan Hydroxylase [AlinoldJ. MandeZl, Suzanne Knapp and Hank A. Gzytn) 663

Effects of Chronic Haloperidol Treatment on Nigral Dopaminergic

Cell Activity (8.5. Bunnty and A.A. GAact) 666

Effects of Chronic Lithium Administration on the Developmentof Supersensitivity in CNS Amine Systems: A MicroiontophoreticStudy [Vonothy W. GalZagtn and William E. Bunnty, Jn.) 669

Alpha-Adrenoreceptor Mediated Inhibition of Brain NoradrenergicNeurons After Acute and Chronic Treatment with Tricyclic Antidepres¬sants [Tongny H. Svznsson and Ted Usdin) 672

Effect of Diazepam on Brain Monoamine Metabolism (B. Biswas

and A. Caxlsson) 675

Role of the Nucleus Locus Coeruleus in Sympathetic and Central

Noradrenergic Activation as Reflected by Changes in the NorepinephrineMetabolite 3-Methoxy-4-Hydroxyphenethyleneglycol (MHPG) in Rats

(J.W. Cnawlty, J.W. Moos and R.H. Roth) 678

Plasma Dopamine Metabolites: Useful Indices of Central

Dopamine Metabolism? [RobeAt H. Roth and Nicholas G. BacopouZos) . .681

The Origins of Plasma Epinephrine, Norepinephrine and DopamineLevels in Stressed Rats (R. Kvetnansky, V.K. Wtlst and I.J. Kopin) .

684

The Neurochemical Profile of a Prodrug of ADTN (A.S. Honn,

V. dt Kastt, V. Vljkstxa, H. Rollema, B.H.C. WtsteXink, J.C. van Otnt,M.G.P. Ftenstna and C.J. Gnol) 687

Correlation of Behavioral and Neurochemical Events Following

Acute and Repeated Administration of Haloperidol [Monk Rodman,JzA&nzy K. Sazlzns, Jt{inty Litbman and Ronald V. Robson) 690

Haloperidol and Apomorphine: Do They Share a Common Site of

Action? [William F. Hexblln) 693

Discrimination Between Haloperidol-Induced Striatal Dopamine

Release and Metabolism (P. Mo-teman, J. BAuinvtZs and C.F.M. van

Malktnbuxg) 695

Dopaminergic Activity Following Acute and Chronic Neuroleptic

Treatment (E. Fnlzdman, E. MeZlex and M. HalZock) 698

FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM

Activation of Tyrosine Hydroxylase and Dopamine Metabolite

Accumulation in the Primate Cingulate Cortex Following Chronic

Haloperidol [Gonzalo Bustos, Nicholas G. Bacopoulos, V. EugznzRedmond, Jn. and Robext H. Roth) 701

The Time Course of Haloperidol-Induced Dopamine Receptor Site

Supersensitivity in Guinea Pigs (W. WtineA, P. CaAvty, A. Hltnl, P.

Nausltdo and H.L. Klawani) 704

The Effects of Metoclopramide and Sulpiride on Dopamine Metab¬

olism in the Rat Brain (M. Stanley, S. WiZk and S. Gtnshon) 707

Phencyclidine Inhibits the Activity of Striatal Tyrosine

Hydroxylase In Vivo [MiZjona Slmonovlc, John Vohtnty, Rtbttca So

and HzAbtnt Y. MtZtzex) 710

Malfunction of Dopamine Systems In Ethanol-Dependent Animals

[Bonis TabakoU, R. F. RitzmaAn and Paulo L. Ho&£man) ; . .713

An Apparent Paradoxical Effect of Blockade and Stimulation of

DA Receptors on Hypothermia in Rats [Shlomo Ythuda) 716

A New Animal Model for Schizophrenia: Behavioral and Receptor

Binding Studies [RichaAd L. Bonlson, Ana Hltxl, Hanold L. KZawans

and Bnuct I. Vlamond ..... 71ft

The Dopamine Neuronal Storage System and Non-Amphetamine

Psychotogenic Stimulants: A Model for Psychosis (P.A. Shont, B.A.

McMiZlzn, H.H. MiZlex, M.K. Sanghexa, R.5. KlstA and V.C. GeAman). . 722

The Mode of Action of Central Stimulatory Agents [Svantt B. RoM)728

Amphetamine Induced Hyperactivity: Newly Synthesized Versus

Stored Catecholamines [Tommy Lz0andZA and Enlk WidzAlbv) 731

Differential Actions of Amphetamine Isomers on A9 and A10

Dopamine Neurons: Correlation with Psychotogenic Effects [S.

Bnovidtn, V.C. Gexman, R.S. Klsex and P.A. Shont) 734

Regional Rat Brain Synthesis and Levels of Catecholamines

After a-Methyl-p_-Tyrosine [Enlk Wldtnlov and Tommy LeiMndtn) .... 737

Biochemical Assessment of the Neuroleptic Activity of

Molindone [8. Ktnntth Kot) . > 740

Central Beta-Adrenergic Receptor Subsensitivity DevelopsAfter Repeated Administration of Antidepressant Drugs (M. StlZingZA,A. Fnaztn and J. Mtndels) 743

Biochemical and Autoradiographic Studies with Reserpine and

Ro 4-1284 in the Rat [Most Va Pnada, John Gnayson Richands, Hans H.KeZZtn and Vtnts Kiss) 746

Section F: Development and Plasticity

Development of the Central Noradrenergic Neurons in the RatBrain [Joseph T. Coylt, Michael V. Johnston and Relnhand Gnzanna). . 751

Histogenesis of Developing Dopamine Neurons in Cell Aggre¬gates In Vitro [BzaiAicz 8. Ganbzn, Gzongz M. Powell, Philip C.

Ho^maHw and Al{,ntd HeZltn) 757

Catecholamine-Containing Neurons from Rat Brain in Culture:

Response to Peripheral and Central Target Tissues (Wm. J. ShozmaktA,M. Schlumpi, B.R. Clank, V. Andtnsson and F.E. Bloom) 760

Reinnervation of Transplants in the Anterior Chamber of the

Eye by the Autonomic Ground Plexus of the Iris [Bonny HoUtA, Vavld

Taylon, Akt Szlgtn, RobeAt Fnttdman and Laxs Olson 763

FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM

Initial Appearance of the Noradrenergic Phenotype in Neuroblasts

of the Rat Embryo [Ina 8. Black, PhiZZippt Cochand and Mtntk

Goldstein) 766

Grafts of Locus Coeruleus and Substantia Nigra in Oculo:

Regulation of CA Fiber Growth into Peripheral and Central Target

Tissues and Establishment of Functional Connections Between

Double Brain Grafts [LaAS Olson, Akt StlgtX, Mothias Aland, Robtnt

Fnzedman, Vavld Taylon and Bonny HoileA) 769

The Role of Cyclic AMP-Dependent Protein Kinase in the Catechol¬

amine Mediated Regulation of Nerve Growth Factor Contained in C6 Glioma

Cells (J.P. Schwantz and E. Costa) 773

Changes in Cardiac Norepinephrine in Spontaneously Hyper¬tensive and Stroke-Prone Rats (J.P. ChaZmtAS, P.R.C. HoWt, J.C.

Pnovls and M.J. Wtst) .776

Development of Autonomic Receptors in the Fetal Mouse Heart

[William R. Rotskt, Fon-Chiu Mia. Chen, and Hznny I. YamamuAa). . . . 779

Developmental Changes in Catecholamine Drug Responses [Loy V.

Lytlt and Edwin Mzyzn, Jn.) 782

Central Regulation of Sympathetic Neuron Development (Robert W.

HamiZl, Emanuel M. Bloom, Philippt V. Cochand and Ina B. Black). . .785

Development of Sympathetic Nerve Supplies to Heart and Adrenal

Medulla of Neonatal Rat (T.A. Slotkln, C. Lau and V.L. Banels) . . . 788

Cardiovascular Responses to Biogenic Amines in Neonatal

Swine [Phyllis M. Gootman, Nancy M. Buckley and Nonman Gootman) . . 791

Immunocytochemical Studies of the Prenatal Development of

the Adrenal Medulla (G. Ttlttlman, H. BaktA, T.H. Joh and V.J. Rtls) 794

Regenerative Sprouting of Noradrenergic Terminals in the

Cerebellum [RichaAd M. Kostnzewa, M. Blain Clank and Hidtkl

Fukishuna) 797

Interactions of Dopamine (DA) and Acetylcholine (ACh) in the

Caudate Nucleus of the Developing Kitten [S.H. ButchtA, M.S. Ltvlnt,

A/.A. Buchwold and C.V. Hull) 800

Functional Development of the Dopaminergic Nigrostrlatal

System [Alined HeZltn, John C. Chtnonis and PhiZlp C. Ho^mann). . 803

Catecholamines in Early Development (M. Schlumpl, W.

Lichtznstzlgex, W.J. Shoemaktn and F.E. Bloom) 806

Catecholamine Innervation of the Developing Neocortex in the

Rat [Pat Ltvltt and Robtnt Y. Moont) 809

Sex Differences in Hypothalamic Monoamine Levels of the

Neonatal Rat [Bextha G. Onttga-Conona, Nona Espanza-Avalos and

Agwstln Soia) 812

Developmental Changes in Catecholamine Content and Activity in

Circumscribed Regions of the Cerebral Cortex in the Rhesus Monkey

(RogeA M. Bxown and Potnlcia S. Goldman) 815

Production of Cholinergic-Adrenergic Cell Lines by Cell Fusion

[Roli Htumann, MilczZZa Ocalan, Volkex Kaehzl and Bfrnd Hampntcht). 818

Increase _In Vivo and In Vitro of Catecholamines and Catechol-

amine-Synthesizing Enzymes in SIF Cells of Newborn Rat SuperiorCervical Ganglia [Olovl Enanko and Llisa Enanko) 821

Target Organ Regulation fti Vitro of the Development of a

Mouse Embryo Sympathetic Ganglion [Michael V. Cougklin and Ina B.

Black) 824

XV i i i FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM

The Initial Findings of the Effects of Fetal and Postnatal

Protein Deprivation on Brain Monoamine Content [Elizabeth M. BuAnS,

Thomas W. Kxuckzbexg, Paul K. Gaeta.no, Jamzs Q. Klssant and LynnE. Comexiond) 827

Ontogenetic Malnutrition and Interaction of Monoamines and

Enzymes in the Brain [Jongt HeXnandtz Rodnlgutz) 830

Age-Related Characteristics of the In-Vitro Accumulation of

3H-NE [Canol Kellogg, Jean VIRaddo and Jamts Roth) 833

Effect of Deafferentation by Olfactory Bulb Ablation on the

Dopamine Neurons in the Olfactory Tubercle During Ontogeny [GostoJonsson and Hakan HalZman) 836

Neuronal and Behavioral Plasticity After Noradrenergic Lesions

in Developing Rat (B.A. Pappos, T.K. Haxdtn, R.B. Mailman, J.H.

Wilson, R.A. MutZltn and G.R. Bnttst) 839

SIF Cells in Decentralized Developing Sympathetic Ganglia

[LUsa Enanko and Olavl Enanko) 842

Effects of Age and Chronic Neuroleptics on Measures of Super¬

sensitivity (Robert C. Smith, Chandna H. Mlsna, Btnt T. Ho and

Lloyd W. Tansty) 845

The Locus Coeruleus Neuroblasts: Plasticity and Function

in Development (J. Sltvtns, H.G. BaumgaAttn, M. Benny, H.P.

Klemm and S. Jtnnzn) 848

Relation Between Destruction and Sprouting of DevelopingCentral Noradrenergic Neurons After Neurotoxic Compounds

[GulZltAmo Jaim-EtchtveAny and Luis Mania Zithzn) 851

Circulating Catecholamines and the Cardiovascular Metabolic

and Endocrine Responses of Fetal Sheep [Colin T. JonzS) 854

Section G: Methods of Analysis

Radioenzymatic Assays for Catecholamines and Related Compounds

[Vavld P. Htnny) 859

A New Sensitive Radioenzymatic-High Pressure Liquid Chroma¬

tographic Method for the Analysis of Plasma Catecholamines [MaxgaXttVtmtny, Agnts K. Klnschnex and N. Enlc Na&tchl). .

862

Are the Radioenzymatic Methods for Catecholamines Convenient

and Usable for Routine Purposes? [Etltnnt Camay and Claudt Bohuon) 865

Radioimmunoassay of Catecholamines [MaSanoxi Yoshloka, Akixa

MlM and Zznzo Tamuxa) 868

A Specific Radioimmunoassay for 3~Methoxy-4-Hydroxyphenylethyl-

Glycol (mopeg) (T. Kent Ktzton, Htnny KAutzsch and Waltex Lovznbzng) 871

Catecholamines Covalently Bound to Proteins. Control of

Quality and Usefulness (Gtaude. Bohuon and Etltnnt Comoy) 874

Biogenic Amine Detection In Vivo and In Vitro: A Combined

Histochemical and X-Ray Analytical Study (F.A. RoSt, J.V. LtVtX

and R.M. Santex) 877

Measurement of In Vivo Decarboxylation of Dopa and Tyrosine[Ulnich Bnaun, Glstla Bnaun and Thonnton Saxgtnt 111) 880

In Vivo Voltammetric Monitoring of Dopamine Release and

Catabolism in the Rat Striatum [RoSS F. Lant, AnthuA T. Hubbondand Chonlts V. Blaha) 883

FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM xix

A Comparison of Monoamine Metabolite Levels in Control and

Perfused Rat Brain [Susan E. Hattox and Jacqutllnz N. Cnawlty) . . 886

Use of Deuterium-Labelled Methionine and HVA to Study Dopamine

Metabolism In Vivo (M. Ebeht, M. Elchlsak, A. Zavadil, R. Poliukyand I. Kopin) 8B9

Studies on the Release of Endogenous Dopamine From Subcellular

Particles Derived from Dendrites of Rat Nigro-Striatal Neurons

(F. Hz&tl and W. LichttnStelgeA) 892

Section H: Plasma Catecholamines

Plasma Catecholamines: A Brief Overview [Inwin J. Kop-i.nl . . 897

Indices of Sympathetic Activity in Hypertensive Rats [Natalit

AltxandeA and Manuel T. \ltZasqutz) 903

Role of Noradrenergic Nerves and Adrenal Medulla During the

Development of Genetic and Experimental Hypertension in Rats

[Honst Gnobzcktn, Juan M. Saavtdna, Richaxd McCanty, Vinginia. K.

Wtlst and Inwin J. Kopin) 906

The Significance of Assessing Circulating Catecholamines

in the Evaluation and Treatment of Hypertensive Patients (Jacqae4dt Champlaln, Vanlzl Cousintau and Lionond Lapointt) 909

Catecholamine Release in the Human Fetus and Newborn Infant

[H. Lagtncnantz, P. Blstalettl and N.O. LuneZl) 912

Plasma Catecholamine, Normetanephrine and p-Octopamine

Levels: Stress- and Drug-Induced Changes in Rat [Most Va. Pnada,Giovanni B. Plcotti, Mlchtlt 0. Cannuba and Willy E. HazieZy).... 915

Psychoactive Drug Effects on Plasma Norepinephrine and Plasma

Dopamine B-Hydroxylase in Man [V.L. MuAphy, C.R. Lake, S. SlateX,

S. Lipptn, V. Shlling, E. dt la Vtga and M.G. lizglzn) 918

Altered Sympathoadrenomedullary Activity in Young Patients

with Suppressed Renin Responsiveness [Vincznt VtQuattAO, Monk

Mtntstn, Ellin LtebeAwm) 921

Direct Stimulation of Sympathetic Outflow and Plasma Levels of

Norepinephrine and Epinephrine of Pithed Rats (I. Vamaguchl and

I.J. Kopin) 924

Plasma Levels of Glycol Metabolites and Presynaptic Metabolism

of Norepinephrine (NE) [V.C. JlmtASon, C.L. Sun, I. Yamaguchi and

I.J. Kopin) 927

Predictability of the Change in Plasma Norepinephrine Con¬

centration in one Vascular Bed Knowing the Change in Plasma Nor¬

epinephrine in Another Vascular Bed [Michael F. Roiztn, John R.

Moyexs, Jwstui A. Zlvln, Philip L. Wilkinson, C. Raymond Lake,

William K. Hamilton, Kanu Chatttnjtt and Richaxd (si. Honnigan).... 930

Rate of Removal of Norepinephrine From the Circulation in

Healthy Men and Patients with Essential Hypertension [MuAAay EilzA,

Viannt KtZlehex, Alex Bobik, Gaxny Jennings and Paul Konnzn) ....933

Changes in Noradrenaline Release Rate and Noradrenaline Storage

Vesicles During Prolonged Activity of Sympathetic Neurones (M. FiZlenz,

S.C. Stan£ond and C.R. Bzntdlct) 936

Hypobaric Stress and Cerebral Blood Flow, Brain and Peripheral

Catecholamines in Rats (M. Le. Poncln-Laiittt, F. BolsmaAZ, P.C.

Ptsqults and J.R. Rap-tn) 939

XX FOURTH INTERNATIONAL CATECHOLAMINE SYMPOSIUM

The Cerebrospinal Fluid Production is Influenced by Sympathetic

Nerves in the Choroid Plexus [Mania Lindvall, Laxs Edvlnsson and

Chnisttn Oman) 942

Prejunctional Adrenoceptors in Rat Peripheral Tissues [ViAtndAOK. Shanma and Shailtsh P.Bantnjzt) 945

Adrenergic Nerve Degeneration [FnanclSto J.E. Stzfiano and

CaAloi J. PtAtc) 948

Circulating Catecholamines Prior to and After Delivery (R. Axtal,

R.W. Lam, J. Eliot, C.J. Hobtl and V.A. Flshex) 951

Section I: Catecholamines and Physiological Processes

The Involvement of the Sympathetic Nervous System in Micturition,

Including Reference to the Use of Imipramine in Enuresis [Michael

V. Cnaggs and John V. Sttphtnson) 957

Metabolism of L-DOPA by Rats After Complete Hepatectomy

[GzntAudt W. Tyce. and Chanlzs A. Owtn, Jn.) 960

Dopamine-Related Tetrahytdroisoquinoline (TIQ) Excretion

by Alcoholics [WiZJUum P. Nijm, Gntgony Teai, Gtongt Bongt, Claxa

Goldianb and Mlchazl A. Collins) 963

Immunohlstochemistry of Tyrosine Hydroxylase (TH) in Human and

Simian Nervous Systems: Alterations in Familial Dysautonomia

(Jonn Pzanson, Ltslit Bnandtls and Mtntk Goldstein) .966

Central Dopaminergic Modulation of the Respiratory Control

System (R.A. MueZleX, G.R. BntzSt and V'. Lundbexg) 969

Decreased Plasma Dopamine-g-Hydroxylase Activity During_Human

Septic Shock (A. Haxanl, A.M. Bou.iu.eA, E. Mantin, E. Comoy and C.

Bohuon) 972

Periodic Fever Suppressed by Reserpine and Colchicine [AkiAO

Hayashi, Tomokazu Suzuki, Akixa Shlmlzu and Yulchl YamamuAa). . . . 974

Pharmacological Characterization of Catecholamine Neurons in

the Rat Sympathetic Ganglion (F. KaAOum, S.G. Sptclalt and R.J. Wyatt\%T]

Role of the Norepinephrine- and Acetylcholine-Sensitive Neurons

of the Hypothalamus in the Regulation of Hepatic Metabolism [TakaShlShimazu and Hixo&hl Matsushita) 980

Mechanisms of Interaction Between ATP and Catecholamines

in Regulation of Smooth Muscle Contractility [Tomlslav Kazlc) . . .983

On the Sympathomimetic Induced Effects on Skeletal Muscle

Contractions (E. Holmbtng, I. SvtdlngeA and B. Waldtck) 986

Adrenergic Influences on Skeletal Muscle Contraction (l/.M.\lanaglc) -.

_

989

Phosphorylation of Smooth Muscle Myosin Light Chain Kinase bythe Catalytic Subunit of Adenosine 3':5'-Monophosphate DependentProtein Kinase [Robznt S. Adelsttin, Many Annt Contl, Vavid R. Hathawayand Claudt B. Kltt) 992

Alterations in Adrenergic Responses After Treatment of Rats

with Desmethylimipramine (DMI) [Manilyn E. HtSS, Alan FhaztA and

Bnadlzy Gabel) 995

Responses to Catecholamines in Pyridine Nucleotide [NAD(P)H]Levels Following Electrical Field Stimulation (Joseph T. Cummlni and

Elizabeth KtZltn) 998

Depletion of Norepinephrine Stores of the Rat Vas Deferens byReserpine In Vitro ['Anun R. Wakadz) 1001