carlopez-khymos-2012 de selectividad columnas en...
TRANSCRIPT
![Page 1: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/1.jpg)
Selectividad de Columnas
en HPLC
Humax Mayo 2012carlo
pez-
khym
os-2
012
![Page 2: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/2.jpg)
Carlos Lopez - udea
Criterios Para comprar Columnas de LC?
carlo
pez-
khym
os-2
012
![Page 3: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/3.jpg)
Variables Affecting HPLC Column Charactersitics
There are very many characteristics of a stationary phase which affect the quality of a separation, not least of which is the chemical nature of the silica (or polymer etc.) stationary phase support particles and the bonded phase ligand. Below we have listed just some of the parameters which need to be considered when choosing, for example, a C18 column.
One should note that all of the factors listed pertain to the same USP stationary phase classification (L1) and indicate the myriad of combinations which account for the marked differences in retention, selectivity and performance between C18 (L1) columns from different manufacturers:
Bonded phase
• Standard octadecylsilyl alkyl silane
• Polar Embedded C18 (with imide, carbamate etc. spacer) for enhanced retention / selectivity of polar analytes
• Mixture of C18 and shorter alkyl chains to give different selectivity / water wettability etc.
• Nature of the silane substituents (e.g di‐isobutyl silane) used as bulky substituents for pH stability at lower pH
• Carbon loading
• Carbon loading to silica surface area ratio (governs the ‘wetability’ of the phase and its ability to retain and separate polar analytes)
carlo
pez-
khym
os-2
012
![Page 4: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/4.jpg)
Nature of the base silica
• Sol or sil‐gel particle
• Type I or Type II silica (governs the surface activity of the silica and the number of available ‘acidic / lone’ silanol groups which ultimately cause peak tailing if not effectively end‐capped)
• Silica metal ion content (effects peak tailing)
• Totally porous, polymeric (effects pH stability) or superficially porous (for increased efficiency) support particles
• Pure silica or organic / inorganic hybrid (to achieve wider pH stability)
• Spherical or irregular silica particle
• Particle size and particle size distribution (including the newer sub 2μm materials)
• Pore size
• Surface area
• Deactivation (end‐capping) post bonding – nature of the end‐capping reagent (volume, polar, non‐polar), end‐capping reaction conditions (determines the extent of end‐capping)
carlo
pez-
khym
os-2
012
![Page 5: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/5.jpg)
Bonded phase
• Column Length
• Internal diameter
• Nature of the material used for column construction (typically stainless steel)
• Metal passivation technique
• Interior tubing surface polishing
• Nature of the frit and spreaders used in the column end fittings
• Packing quality (including method of packing, packing pressure, solvents used etc.)
carlo
pez-
khym
os-2
012
![Page 6: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/6.jpg)
El ayer..y el hoy….
UHPLC
carlo
pez-
khym
os-2
012
![Page 7: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/7.jpg)
carlo
pez-
khym
os-2
012
![Page 8: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/8.jpg)
Silicas Totalmente porosas vs silicas superficialmente porosas
carlo
pez-
khym
os-2
012
![Page 9: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/9.jpg)
SEM Kinetex Core Shellca
rlope
z-kh
ymos
-201
2
![Page 10: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/10.jpg)
Columnas Core Shellca
rlope
z-kh
ymos
-201
2
![Page 11: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/11.jpg)
carlo
pez-
khym
os-2
012
![Page 12: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/12.jpg)
carlo
pez-
khym
os-2
012
![Page 13: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/13.jpg)
carlo
pez-
khym
os-2
012
![Page 14: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/14.jpg)
Core Shell – Difusion Eddyca
rlope
z-kh
ymos
-201
2
![Page 15: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/15.jpg)
Core Shell ‐ Difusiónca
rlope
z-kh
ymos
-201
2
![Page 16: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/16.jpg)
Columnas Chromolith
Starting Sol
Phase Separationand Gelation
Ageing Drying
Cladding
HPLC
Preparacion
Si(OAlk)4 Polymer
H+
carlo
pez-
khym
os-2
012
![Page 17: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/17.jpg)
Carlos Lopez - udea
Caracteristicas de las columnas de silica monolitica
carlo
pez-
khym
os-2
012
![Page 18: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/18.jpg)
Carlos Lopez - udea
25 mm10 mm
4.6mm
Columnas de silica Monolitica
3.0 mm
carlo
pez-
khym
os-2
012
![Page 19: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/19.jpg)
Carlos Lopez - udea
Chromolith® Performance
p/u curves for Chromolith® and particulate HPLC columns
0
50
100
150
200
250
0 2 4 6 8Flow rate (mL/ min)
Col
umn
back
-pre
ssur
e (b
ar)
Column X: 5mColumn S: 5mColumn Z: 5m
Presión –Columnas de 5µm
carlo
pez-
khym
os-2
012
![Page 20: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/20.jpg)
Columna de 1.4 metros…!!!!!!
carlo
pez-
khym
os-2
012
![Page 21: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/21.jpg)
carlo
pez-
khym
os-2
012
![Page 22: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/22.jpg)
USP Designations – Agilent (1)
carlo
pez-
khym
os-2
012
![Page 23: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/23.jpg)
Columnas LC – Agilent (2)
carlo
pez-
khym
os-2
012
![Page 24: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/24.jpg)
Columnas LC ‐ Agilent (3)
carlo
pez-
khym
os-2
012
![Page 25: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/25.jpg)
Columnas LC‐ Agilent (4)
carlo
pez-
khym
os-2
012
![Page 26: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/26.jpg)
Columnas LC Aplicaciones Agilent (4)
carlo
pez-
khym
os-2
012
![Page 27: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/27.jpg)
Columnas LC Agilent Aplicaciones ‐ Agilent (5)
carlo
pez-
khym
os-2
012
![Page 28: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/28.jpg)
USP Designations – Waters (1)
carlo
pez-
khym
os-2
012
![Page 29: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/29.jpg)
USP Designations – Waters (2)
carlo
pez-
khym
os-2
012
![Page 30: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/30.jpg)
USP Designations – Waters (3)
carlo
pez-
khym
os-2
012
![Page 31: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/31.jpg)
USP Designations – Waters (4)
carlo
pez-
khym
os-2
012
![Page 32: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/32.jpg)
Columnas Merck
carlo
pez-
khym
os-2
012
![Page 33: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/33.jpg)
carlo
pez-
khym
os-2
012
![Page 34: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/34.jpg)
carlo
pez-
khym
os-2
012
![Page 35: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/35.jpg)
carlo
pez-
khym
os-2
012
![Page 36: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/36.jpg)
carlo
pez-
khym
os-2
012
![Page 37: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/37.jpg)
Como comparar columnas?ca
rlope
z-kh
ymos
-201
2
![Page 38: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/38.jpg)
USP Columns
http://www.usp.org/USPNF/columns.html
carlo
pez-
khym
os-2
012
![Page 39: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/39.jpg)
Chelating Tailing FactorCapacity Factor Amitryptyline
Bonding Density
Tailing Factor Amitryptyline
carlo
pez-
khym
os-2
012
![Page 40: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/40.jpg)
Examples of separations of SRM 870 on commercial C18 columns ‐ highly deactivated Type B silica with polar embedded ligand (top), high silanol and metal ion activity phase (bottom).
carlo
pez-
khym
os-2
012
![Page 41: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/41.jpg)
Shape selectivity (bonding density)
The term ‘shape selectivity’ is used to denote a chromatographic quality exhibited by certain stationary phases for which enhanced separations of geometric isomers may result based on their molecular structure rather than other physical or chemical properties of the solute. SRM 870 does not characterize shape selectivity, however this property can be assessed by use of other chromatographic test mixes, such as SRM 869a
[http://ts.nist.gov/MeasurementServices/ReferenceMaterials/archived_certificates/869a.pdf], Column Selectivity Test Mixture for Liquid Chromatography. This test uses benzo[a]pyrene (BaP), phenanthro[3,4‐c]phenanthrene(PhPh) and 1,2:3,4:5,6:7,8‐tetrabenzonaphthalene (TBN) as probes and the shape selectivity measurement is the selectivity between TBN and BaP. The structures and space filling models of these compounds are shown in Figure 23.
Testing columns for shape selectivity according to the USP classification model.carlo
pez-
khym
os-2
012
![Page 42: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/42.jpg)
carlo
pez-
khym
os-2
012
![Page 43: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/43.jpg)
carlo
pez-
khym
os-2
012
![Page 44: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/44.jpg)
http://www.waters.com/waters/promotionDetail.htm?id=10048475&ev=10058108&locale=en_US
Versión on‐lineca
rlope
z-kh
ymos
-201
2
![Page 45: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/45.jpg)
Several attempts have been made to produce a “definitive” set of chemical probes and tests to best characterize the huge number of stationary phases available. This number was over 600 in the latest report we could find from 2003, and we speculate that well over 1000 different types are currently available. As yet a harmonized set of test probes and methodologies has not been identified.
An early attempt at producing a generic set of probes for testing HPLC column characteristics was made by Tanka and co‐workers and since then work by the USP Working Group on HPLC Columns, the Impurities Working Group of the PQRI Drug Substance Technical Committee in collaboration with Dr Lloyd Snyder and work carried out by Euerby and Petersson to expand the original probes designed by Tanaka have all undertaken to identify a definitive set of probes which will allow the various important physico‐chemical phase characteristics to be specified. Most of these groups have also combined their data with various chemometric approaches to produce quantitative databases based on principal component analysis (PCA) or tools to visualize the relative groupings of commercially available columns according to their key descriptors.
Spider diagram representing the various characteristics of the stationary phase.
HR ‐ hydrophobic retentionHS ‐ hydrophobic selectivitySS ‐ steric selectivityHBC ‐ hydrogen bonding capacityBA ‐ base activityC ‐ chelationIEX ‐ ion exchange capacity at pH2.6 and 7.6AI ‐ acid integration
carlo
pez-
khym
os-2
012
![Page 46: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/46.jpg)
Desarrollo de métodos Automatizados
http://www.molnar-institute.com/carlo
pez-
khym
os-2
012
![Page 47: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/47.jpg)
carlo
pez-
khym
os-2
012
![Page 48: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/48.jpg)
Desarrollo de métodos Automatizados LC y GC
http://www.acdlabs.com/products/com_iden/meth_dev/lc_sim/carlo
pez-
khym
os-2
012
![Page 49: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/49.jpg)
Desarrollo de métodos Automatizados
ChromSword Auto Method Development Software
AgilentAutomated HPLC Method Development: ChemStation with ChromSword
http://www.chromsword.de/
carlo
pez-
khym
os-2
012
![Page 50: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/50.jpg)
Desarrollo de métodos Automatizados
OSIRISHPLC dvelopment Method Software
http://www.kromatek.co.uk/productlist.asp?CatID=88carlo
pez-
khym
os-2
012
![Page 51: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/51.jpg)
http://www.smatrix.com/carlo
pez-
khym
os-2
012
![Page 52: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/52.jpg)
http://www.chem.agilent.com/en‐US/Events/en‐US/Pages/14736B.aspx
Agilent - eSeminars
carlo
pez-
khym
os-2
012
![Page 53: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/53.jpg)
El ayer..y el hoy….
UHPLC
carlo
pez-
khym
os-2
012
![Page 54: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/54.jpg)
Carlos Lopez - udea
Ventajas de tamaño de partícula pequeño
carlo
pez-
khym
os-2
012
![Page 55: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/55.jpg)
carlo
pez-
khym
os-2
012
![Page 56: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/56.jpg)
carlo
pez-
khym
os-2
012
![Page 57: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/57.jpg)
carlo
pez-
khym
os-2
012
![Page 58: carlopez-khymos-2012 de Selectividad Columnas en …quimica.udea.edu.co/~carlopez/hplc/6-column-slectivity-humax-2012.pdf · between C18 (L1) columns from different manufacturers:](https://reader030.vdocuments.mx/reader030/viewer/2022012920/5b93c7c509d3f252738b6f43/html5/thumbnails/58.jpg)
http://gradworks.umi.com/3440078.pdf carlo
pez-
khym
os-2
012