carbon dioxide mitigation by nannochloropsis oculata towards biodiesel production
TRANSCRIPT
Name : Vijendren Krishnan
Chairman : Prof. Dr. Y. Uemura
(30 April 2014)
Research Progress
Carbon dioxide mitigation by
Nannochloropsis oculata
towards biodiesel production
Domain : Eukaryota
Kingdom : Chromalveolata
Phylum : Heterokontophyta
Class : Eustigmatophyceae
Family : Eustigmataceae
Genus : Nannochloropsis
Species : oculata
(Hibbert, 1981)
Taxonomy :
Nannochloropsis oculata (Hibbert)
• Small spheres with diameter of 2 – 4 µM
• One single Chloroplast without pyrenoid, butcontain several lamella, each with 3thylakoids.
• Has polysaccharide cell walls
• Do not accumulate starch
• Non-motile microalgae (non flagellate)(Boussiba et al., 1987)
Morphology
• Not distinguishable by morphologicalcharacteristics, only by molecular technique.
• Marine environment, but other species are alsofound in fresh water and brackish water
(Fawley and Fawley, 2007)
• Contain pigments; Chl α, β-carotene,xantophylls, violaxanthin and vaucherxantinbut lack chl β. (Cohen 1999)
Location of sampling
Teluk Batik Beach
Marina Cove Resort
Titi Panjang
(Lumut Jetty)
Sample Location Google GPS coordinate
1 Teluk Batik Beach N 4o 11’ 20.4”, E 100o 36’ 20.8”
2 Marina Cove Resort N 4o 12’ 48.06”, E 100o 36’ 08.1”
3 Titi Panjang (Lumut Jetty) N 4o13’ 56.0”, E 100o 38’ 31.6”
Location Coordinates
Table 1 : Location and coordinates of microalgae sampling.
A
B
C
Figure 1 : Growth of microalgae in F2 medium. A: 1 day, B: 4 days, C: 6 days
Figure 3 : Growth of microalgae in F2 medium after subculture (8 days)
Figure 2 : Nannochloropsis oculata Observation on microscope at
1000x magnification.
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Day 8
Day 9
Day 10
Day 11
Day 12
1. Hibberd, (1981). Notes on the taxonomy and nomenclature ofthe algal classes Eustigmatophyceae and Tribophyceae(Synonym Xanthophyceae). Botanical journal of the Linneansociety, 82: 93-119.
2. Boussiba, S., Vonshak, A., Cohen, Z., Avissar, I., and Richmond,A. (1987). Lipid and biomass production by the halotolerantmicroalga Nannochloropsis salina. Biomass, 12: 37-47.
3. Fawley, K. P., and Fawley, M. W. (2007). Observations on thediversity and ecology of freshwater Nannochloropsis(Eustigmatophyceae) with descriptions of new taxa. Protist, 158(3) : 325 -336.
4. Cohen, Z., (1999). Chemicals from microalga, Taylor andFrancis, London, UK.
References