capillary electrophoresis by sunil budke
DESCRIPTION
This was a small presentation I made to the class on Capillary electrophoresis. The system shown here is the one I used at Therapeutic Drug Monitoring Center - Sion Mumbai. It is a auto timed presentation where slides and points keep changing in the background at preset timings in Sync with my talk. I know that without the talk this slide show may not be that useful but still I thought of sharing it with you guys. Hope you can make something out of it.TRANSCRIPT
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CAPILLARY ELECTROPHORESIS
BASIC CONCEPTS & HARDWARE
by Sunil Budke
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CE by SUNIL BUDKE 04/08/23 11:16
Source vial
Destination Vial
Capillary tube
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CE by SUNIL BUDKE 04/08/23 11:16
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CE by SUNIL BUDKE 04/08/23 11:16
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CE by SUNIL BUDKE 04/08/23 11:16
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CE by SUNIL BUDKE 04/08/23 11:16
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CE by SUNIL BUDKE 04/08/23 11:16
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CE by SUNIL BUDKE 04/08/23 11:16
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Electropherogram from CE
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CAPILLARY Made of fused silica Polyamide coating for protection Pyrex or Teflon capillaries are available Effective length 10 - 100 cm Internal diameter (ID) 20 -100 micro meter C8 and C18 coated capillaries are
available
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SAMPLE INTRODUCTION
Electrokinetic Injection5kv for 5 – 10 sec
Gravimetric (Syphonic)Sample vial held at higher level
Differential pressurePressure applied on sample vial or vacuum applied on
Destination vial
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DETECTORS Conductivity Detectors
UV Detectors
Fluorescence Detector
Electrochemical Detector
Mass Spectrometer
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CE by SUNIL BUDKE 04/08/23 11:16
DISCUSSION
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References Capillary Electrophoresis
-by Dale R. Baker
Capillary Electrophoresis for Food Analysis – Method Development
-by Richard A. Frazier; Jennifer M. Ames and Harry E. Nursten
Principles and Practice of Bioanalysis -by Richard F. Venn
McGraw Hill - Year book of Science and Technology – 2002-Capillary electrophoresis
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Thank You