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CHAIR: DR CATHARINE CHISHOLM

48 Abstracts / Contact Lens &

nce is reasonably robust with respect to the day of week on whichhe assessment occurs, older participants may be more likely toomplete all surveys than younger participants.

ttp://dx.doi.org/10.1016/j.clae.2012.10.053

an the effects of collagen cross-linking on human corneal cellopulation in-vitro?

thar M. Beshtawi 1,∗, Clare O’Donnell 2, Chantal Hillarby 3, Arunrahma 4, Fiona Carley 1,2,3,4, Hema Radhakrishnan 1,2,3,4

Faculty of Life Sciences, University of Manchester, Manchester, UKOptegra, Didsbury, Manchester, UKFaculty of Medicine, University of Manchester, Manchester, UKManchester Royal Eye Hospital, Manchester, UK-mail address: Ithar.Beshtawi@postgrad.manchester.ac.uk (I.M.eshtawi).

Purpose: Corneal collagen cross-linking is a recently developedrocedure to treat keratoconus. Research has been conducted totudy the effects of treatment; in-vitro studies have been mainlyone using animal models. We explored the feasibility of using eye-anked human corneas to examine the effects of collagen cross-

inking on corneal cells in-vitro.Method: Two corneas were used, one cornea was crosslinked

ccording to the standard protocol and the other cornea wasxposed to riboflavin only as a control. Confocal microscopy wassed to image the corneal layers and assess the endothelial cell andtromal keratocyte morphometry. TUNEL technique was used tossess apoptosis of the corneal cells.

Results: The human cornea was successfully treated. Addition-lly we were able to collect morphometric data for the keratocytesnd endothelial cells in both corneas. Anterior keratocyte densityas 1037.6 ± 90.2 and 1379.5 ± 214.6 cells/mm2 in the cross-

inked and control cornea respectively. Posterior keratocyte densityas 432.7 ± 10.9 and 455.1 ± 58.4 cells/mm2 in the cross-linked

nd control cornea respectively and endothelial cell density waseasured as 1744.6 ± 178.8 and 2282.9 ± 132.6 cells/mm2 in the

ross-linked and control cornea respectively. Apoptosis detectiony the TUNEL technique showed positive staining in the anteriorortion of the cross-linked cornea only. No apoptotic nuclei wereetected at any level in the control cornea.

Conclusions: Human corneas in organ-culture can be success-ully cross-linked allowing the effects of this treatment to bessessed in-vitro. The techniques developed in this preliminarytudy provide a model for assessing the effects of treatment andevised cross-linking protocols on layers of the human cornea in-itro.

ATURDAY MAY 26, 2012 CONFERENCE SESSION 21: PRACTICALONTACT LENSES, HALL 5, 09:30–10.30CCREDITED FOR 1 CL CET – DISPENSING OPTICIANS/CONTACTENS SPECIALIST/OPTOMETRISTS COPE: 34134-CLHAIR: JUDITH MORRIS

ttp://dx.doi.org/10.1016/j.clae.2012.10.054

aming torics and attacking astigmatism

ark Andre ∗, Patrick Caroline-mail address: andrem@pacificu.edu (M. Andre).

This lecture will examine the issue of how the astigmatic FAAOatient is managed with toric soft contact lenses. We will discussontemporary fitting techniques that allow for a Pacific University

or Eye 35S (2012) e33–e50

College of Optometry Forest Grove, more precise fitting of toric softlenses on the astigmatic Oregon, USA patient.

SATURDAY MAY 26, 2012 CONFERENCE SESSION 22: PRACTICALCONTACT LENSES CONT’D, HALL 5, 11:00–11.50CHAIR: JUDITH MORRIS

http://dx.doi.org/10.1016/j.clae.2012.10.055

Modern materials and their clinical implications

Ian Pyzer 1,2

1 Johnson & Johnson, UK2 Johnson & Johnson, IrelandE-mail address: ipyzer@its.jnj.com.

In order to improve patient satisfaction with soft contact lenses,we need to better understand the various factors affecting theirperformance. Differences in material characteristics which affectpatient experience will be discussed in detail, concentrating onthose which have a direct bearing on comfort and discontinua-tion while also dispelling myths about others that do not. Oxygen,modulus, wettability, coefficient of friction, water content as wellas differences in UV protection will be explored in this lecture.Understanding how the laboratory measurements for some of thesethe material properties are achieved including those that do notcurrently have an ISO standard, will be explored to allow practi-tioners to appreciate the methodology behind the data. The lecturewill help the eye care professional considering both their new andexisting wearers, to better appreciate how differences in design &material can provide significant benefits.

SATURDAY MAY 26, 2012 CONFERENCE SESSION 23: PRACTICALCONTACT LENSES CONT’D, HALL 5, 14:30–16.00ACCREDITED FOR 1 CL CET – DISPENSING OPTICIANS/CONTACTLENS SPECIALISTS/OPTOMETRISTSCHAIR: NIGEL BURNETT HODD

http://dx.doi.org/10.1016/j.clae.2012.10.056

Getting started: A simple guide to fitting the keratoconic patient

Paul Rose

Visique Total Vision, New ZealandE-mail address: rose@rosek.co.nz.

The lecture will take the fitter through a step by step fitting of theirregular cornea by firstly identifying the type of irregular corneaby correct interpretation of topographies, then choosing the correctdesign and the reasons why design is so important to maximisesuccess rate. Then a 5 step fitting system will be illustrated whichwill approach the fitting in a systematic way. At each of the 5 steps,rules will be given to assist the fitter with choosing the correctparameters to maximise their success rate. Also fitting tips will begiven to overcome fitting issues and problems.

SATURDAY MAY 26, 2012 CONFERENCE SESSION 24: PRACTICALGRAND ROUND, HALL 6, 16:30–17.30

http://dx.doi.org/10.1016/j.clae.2012.10.057