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10 5 10 5 buffer β- actin (ng/ml) wt ΔC12 WT ∆C12 20 25 kDa CBB stained 10 15 A B Supplementary Figure S14 Supplementary Figure S14. L1-RTP by Vpr required a carboxy- terminal region. A. Purification of rVpr and ΔC12. Each rVpr were purified using two-step column chromatography at the same time. Purified proteins were stained with Coomassie brilliant blue (CBB). B. Dependence of rVpr induced L1-RTP on its C-terminal region. HuH-7 cells were transfected with pEF06R and selected with Puro. Replated cells were treated with indicated amouts of either Wt or ΔC12 of rVpr for 2days. rVpr induced L1-RTP was analyzed by PCR based assay. Apparent L1-RTP induction was not observed by C-terminus truncated rVpr treatment.

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Supplementary Figure S14. A. B. kDa. WT. ∆C12. buffer. wt. ΔC12. (ng/ml). 10. 5. 10. 5. 25. 20. 15. β-actin. 10. CBB stained. Supplementary Figure S14. L1-RTP by Vpr required a carboxy-terminal region. - PowerPoint PPT Presentation

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Page 1: buffer

10 5 10 5bu

ffer

β-actin

(ng/ml)

wt ΔC12

WT ∆C12

2025

kDa

CBB stained

10

15

A B

Supplementary Figure S14

Supplementary Figure S14. L1-RTP by Vpr required a carboxy-terminal region.A. 　 Purification of rVpr and ΔC12. Each rVpr were purified using two-step column chromatography at the same time. Purified proteins were stained with Coomassie brilliant blue (CBB). B. Dependence of rVpr induced L1-RTP on its C-terminal region. HuH-7 cells were transfected with pEF06R and selected with Puro. Replated cells were treated with indicated amouts of either Wt or ΔC12 of rVpr for 2days. rVpr induced L1-RTP was analyzed by PCR based assay. Apparent L1-RTP induction was not observed by C-terminus truncated rVpr treatment.

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