BSc Clinical GeneticsBSc Clinical GeneticsMy story!My story!

Donna WoodmanDonna Woodman

MTO2MTO2

BSc Clinical GeneticsBSc Clinical Genetics

September 2002 – enrolled at Wolverhampton September 2002 – enrolled at Wolverhampton University for BSc Clinical Genetics Course.University for BSc Clinical Genetics Course.

4 year course.4 year course.

1 day per week.1 day per week.

5 days study leave per year.5 days study leave per year.

11stst year modules year modules

Biochemistry Biochemistry

Human Form Human Function Human Form Human Function

Human Health, Disease Prevention and Human Health, Disease Prevention and TreatmentTreatment

Human Physiology Human Physiology

Molecules and Cells in Health and Disease Molecules and Cells in Health and Disease

Professional Studies in Healthcare Science Professional Studies in Healthcare Science

22ndnd year modules year modules

15 and 30 credit modules. 15 and 30 credit modules.

Biology of Disease Biology of Disease

Molecular and Cellular Biosciences (30 credits - Molecular and Cellular Biosciences (30 credits - year)year)

Biomedical Science Practicals: Microbiology, Biomedical Science Practicals: Microbiology, Genetics, Biochemistry.Genetics, Biochemistry.

33rdrd and 4 and 4thth year year

1.5 days per week.1.5 days per week.

1 day at university and ½ day in lab (Honours 1 day at university and ½ day in lab (Honours Project).Project).

Advanced Topics in Healthcare Science Advanced Topics in Healthcare Science Evidence-based Practice Evidence-based Practice Gene Manipulation Gene Manipulation Medical Genetics Medical Genetics Biomedical ethicsBiomedical ethics

Honours ProjectHonours Project

Compare Hams, Changs’ and Amniomax Compare Hams, Changs’ and Amniomax for Solid Tissue Culture.for Solid Tissue Culture.

? Difference between media for ? Difference between media for establishing and maintaining cell growth.establishing and maintaining cell growth.

3 extra flasks were set up on each sample 3 extra flasks were set up on each sample with each of the different media.with each of the different media.

Cell counts using a haemocytometer at 12, Cell counts using a haemocytometer at 12, 15 and 19 days prior to harvest.15 and 19 days prior to harvest.

Compared mitotic index, length and Compared mitotic index, length and spreading post harvest.spreading post harvest.

ResultsResults

43 samples were tested43 samples were tested14 samples failed to grow in culture14 samples failed to grow in culture3 infected.3 infected.

Preliminary results: Amniomax established Preliminary results: Amniomax established and maintained both skin and villi cultures and maintained both skin and villi cultures quickest, followed by Changs and then quickest, followed by Changs and then Hams F10 (control) in both skin and villi Hams F10 (control) in both skin and villi samples.samples.

ResultsResults

Related Measures Analysis of Variance Related Measures Analysis of Variance statistical analysis (ANOVA) showed no statistical analysis (ANOVA) showed no significant difference between the medias significant difference between the medias (culturing).(culturing).

The Friedman statistical test: No significant The Friedman statistical test: No significant difference between the media with respect to difference between the media with respect to slide quality.slide quality.

More samples need to be tested which may then More samples need to be tested which may then show a significant difference between the three show a significant difference between the three types of media.types of media.

Further studiesFurther studies

Set up samples in mixture of media.Set up samples in mixture of media.

Establish cultures in one type of media Establish cultures in one type of media and then continue to harvest stage in and then continue to harvest stage in another.another.

Use different hypotonic.Use different hypotonic.

Use different ratio of fixative.Use different ratio of fixative.

Use different concentration of BRDU for Use different concentration of BRDU for harvests. harvests.

Advantages of part time degreeAdvantages of part time degree

Obtain a degree whilst continually gaining Obtain a degree whilst continually gaining experience at work.experience at work.

Gain background knowledge.Gain background knowledge.

Professional development.Professional development.

DisadvantagesDisadvantages

Can disrupt rotas within the lab as Can disrupt rotas within the lab as university days change each semester.university days change each semester.

Hard work!Hard work!