bruker pulse program catalogue - ciencias.ula.ve · d1 presat off-reson. d1 presat pl9 d0 d0 pl12...

BRUKER

PULSE PROGRAM

CATALOGUE

2D HSQC EXPERIMENTS

©2004: Bruker BioSpin , Teodor Parella 147

Pulse Program Catalogue

NMRGuide4.0


�

�

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Phase-cycled:

Phase-cycled and solvent suppression:

Gradient-based:

Phase-sensitive 2D HSQC ( )

Phase-sensitive 2D HSQC with presaturation ( )Phase-sensitive 2D HSQC with off-resonance presaturation ( )

Phase-sensitive ge-2D HSQC using z-filter and selection before t1 ( | )Phase-sensitive ge-2D HSQC using z-filter and selection after t1 ( )Phase-sensitive ge-2D HSQC using echo-antiecho ( | )Phase-sensitive ge-2D HSQC using echo-antiecho and adiabatic pulses for inversion( )Phase-sensitive ge-2D HSQC using echo-antiecho and adiabatic pulses for inversion andrefocusing( )Phase-sensitive ge-2D HSQC using echo-antiecho and adiabatic pulses for inversion andrefocusing and BS effects( )Phase-sensitive ge-2D HSQC using PEP ( )Phase-sensitive ge-2D HSQC using PEP with gradients in back-inept ( )Phase-sensitive ge-2D HSQC using PEP and adiabatic pulses for inversion( |

)Phase-sensitive ge-2D HSQC using PEP and adiabatic pulses for inversion with gradients inback-inept ( )Phase-sensitive ge-2D HSQC using PEP and adiabatic pulses for inversion and refocusing( | )Phase-sensitive ge-2D HSQC using PEP and adiabatic pulses for inversion and refocusing withgradients in back-inept ( )

ge-2D H-X HSQC experiment with X-Y-decoupling during acquisition and withselective Cb/C=O decoupling. ( )

hsqcph

hsqcphprhsqcphps

hsqcgpphhsqcgpph2

hsqcetgp

hsqcetgpsp

hsqcetgpsp.2

hsqcetgpsp.3hsqcetgpsi

hsqcetgpsi2hsqcetgpsisp

hsqcetgpsisp2

hsqcetgpsisp.2

hsqcetgpsisp2.2

hsqcdhetgpsp

HSQCGP

HSQCETGP

HSQCETGPSISP

HSQCETGPSISP.2

1

FROM F2 CHANNEL


NMRGuide4.0


FROM F3 CHANNEL

�

�

�

�

Phase-cycled:

Phase-cycled and solvent suppression:

Gradient-based:

Gradient-based and solvent suppression

Phase-sensitive 2D H- N HSQC ( )

Phase-sensitive 2D H- N HSQC using presaturation ( )

Phase-sensitive ge-2D H- N HSQC using echo-antiecho ( | )

Phase-sensitive ge-2D H- N HSQC using PEP ( | )

Phase-sensitive ge-2D H- N HSQC using PEP with gradients in back-inept( )

Phase-sensitive ge-2D H- N HSQC using XY16-CPMG()

Phase-sensitive ge-2D H- N HSQC using water flip-back and echo-antiecho( | )

Phase-sensitive ge-2D H- N HSQC using water flip-back and PEP ( |)

Phase-sensitive ge-2D H- N HSQC using water flip-back and PEP with gradients inback-inept ( )

Phase-sensitive ge-2D H- N HSQC using WATERGATE (3-9-19) ( )

Fast-HSQC, Phase-sensitive ge-2D H- N HSQC using WATERGATE (3-9-19)( | )

Phase-sensitive ge-2D H- N HSQC using water flip-back and WATERGATE(selective pulse) ( | )

1 15

1 15

1 15

1 15

1 15

1 15

1 15

1 15

1 15

1 15

1 15

1 15

hsqcf3ph

hsqcf3phpr

hsqcetf3gp

hsqcetf3gpsi

hsqcetf3gpsi2

hsqcetf3gpxy,hsqcetf3gpxy.2

hsqcetfpf3gp

hsqcetfpf3gpsi

hsqcetfpf3gpsi2

hsqcf3gpph19

fhsqcf3gpph

hsqcfpf3gpphwg

HSQCETF3GP

HSQCETF3GPSI

HSQCETFPF3GP

HSQCETFPF3GPSI

FHSQCF3GPPH

HSQCFPF3GPPHWG


NMRGuide4.0

�

GARP

�X

1H

Gz

�1

y�rec

� �

�

GARP

�X

1H

Gz

�1

y�rec

��

hsqcgpph

d4

G3

d4 d4 d4

d0 d0

G1 G2

hsqcgpph2

d4 d4 d4 d4

G3G1 G2

d0 d0

d0 d0d0 d0

pl12

pl12

GARPX

1H

1H

�1�1

y

�rec�rec

hsqcph

d4

d0 d0

d4 d4 d4

�

hsqcf3ph

GARPX

1H

1H

�1�1

y

�rec�rec

hsqcphpr

d4 d4 d4 d4

�

hsqcf3phpr

d1

presat

pl9

hsqcphps

GARPX

1H

1H

�1�1

y

�rec�recd26

d0 d0 �

d26 d26 d26

GARPX

1H

1H

�1�1

y

�rec�recd26

d0 d0 �

d26 d26 d26

GARPX

1H

1H

�1�1

y

�rec�recd4 d4 d4 d4

�

d1

presat

off-reson.

d1

presat

pl9

d0 d0

pl12

pl12

pl12

pl12

pl12

©2004: Bruker BioSpin , Teodor Parella

d0 d0

150


NMRGuide4.0

d1

d1

d1

d1

�

GARP

�X

1H

1H

GzGz

�1�1

y�rec�rec

� �

�

GARP

�X

1H

1H

GzGz

�1�1

y�rec�rec

��

hsqcgpph

d4

G3

d4 d4 d4

d0 d0

G1 G2

hsqcgpph2

d4 d4 d4 d4

G3G1 G2

d0 d0

d0 d0d0 d0

1JCH

C C C N C C N

H H HH

C

H H


ppm

10 9 8 7 6 5 4 3 2 1 ppm

20

40

60

80

100

120

140

151

pl12

pl12

GARPX

1H

�1

y

�rec

hsqcph

d4

d0 d0

d4 d4 d4

�

hsqcf3ph

GARPX

1H

�1

y

�rec

hsqcphpr

d4 d4 d4 d4

�

hsqcf3phpr

d1

presat

pl9

hsqcphps

GARPX

1H

�1

y

�recd26

d0 d0 �

d26 d26 d26

GARPX

1H

�1

y

�recd26

d0 d0 �

d26 d26 d26

GARPX

1H

�1

y

�recd4 d4 d4 d4

�

d1

presat

off-reson.

d1

presat

pl9

d0 d0

pl12

pl12

pl12

pl12

pl12

d0 d0


NMRGuide4.0

d1

d1

d1

d1

hsqcetgpsp.3

�

GARP

�

1H

�1

y

�recd4 d4 d4 d4

d0 d0

p28

Gz

G1

G2

15N

13C

p14

sp3

p14

sp3

p14

sp3

p14

sp3

pl12

optional

�

GARP

�

1H

�1

y

�recd4 d4 d4 d4

d0 d0

hsqcdhetgpsp

p28

Gz

G1 G2

13C�

p14

sp313 13C / CO�

p14

sp9

p5m4sp180.p31

G3

p14

sp3

pl12

pl13

�

GARP

�

1H

1H

�1�1

y


d0 d0

hsqcetgp

p28

GzGz

G1

G2

15N

15N

13C

13C �

GARP

�

1H

1H

�1�1

y


d0 d0

p28

GzGz

G1

G2

15N

15N

13C

13C

pl12

optional

�

GARP

�

1H

1H

�1�1

y


d0 d0

hsqcetgpsp

p28

GzGz

G1

G2

15N

15N

13C

13C

p14

sp3

p14

sp3

p14

sp3

p14

sp3pl12

optional

hsqcetgpsp.2

�

GARP

�

1H

1H

�1�1

y


d0 d0

p28

GzGz

G1

G2

15N

15N

13C

13C

p14

sp3

p14

sp3p24

sp7

p24

sp7

p14

sp3

p14

sp3

pl12

optional



NMRGuide4.0

d1

d1

d1

d1

d1

hsqcetgpsp.3

�

GARP

�

1H

1H

�1�1

y


d0 d0

p28

GzGz

G1

G2

15N

15N

13C

13C

p14

sp3

p14

sp3

p14

sp3

p14

sp3

p14

sp3

p14

sp3

p14

sp3

p14

sp3

pl12

optional

�

GARP

�

1H

1H

�1�1

y


d0 d0

hsqcdhetgpsp

p28

GzGz

G1 G2

13C�

13C�

p14

sp3

p14

sp313C /�

13CO

13 13C / CO�

p14

sp9

p14

sp9

p5m4sp180.p31

G3

p14

sp3

p14

sp3

pl12

pl13


�

GARP

�

1H

�1

y

�recd4 d4 d4 d4

d0 d0

hsqcetgp

p28

Gz

G1

G2

15N

13C �

GARP

�

1H

�1

y

�recd4 d4 d4 d4

d0 d0

p28

Gz

G1

G2

15N

13C

pl12

optional

�

GARP

�

1H

�1

y

�recd4 d4 d4 d4

d0 d0

hsqcetgpsp

p28

Gz

G1

G2

15N

13C

p14

sp3

p14

sp3pl12

optional

hsqcetgpsp.2

�

GARP

�

1H

�1

y

�recd4 d4 d4 d4

d0 d0

p28

Gz

G1

G2

15N

13C

p14

sp3p24

sp7

p14

sp3

pl12

optional


NMRGuide4.0

d1

d1

d1

d1

d1

�

GARP

�

1H

Gz

� �

��1

y y�rec

d4 d4 d24 d24

G1

G2

d0 d0

p28

15N

13C

d4 d4

hsqcetgpsisp2

p14

sp3

G3 G3 G4 G4

p14

sp3

pl12

optional

�

GARP

�

1H

Gz

� �

��1

y y�rec

d4 d4 d24 d24

G1

G2

d0 d0

p28

13C

d4 d4

hsqcetgpsisp2.2

p14

sp3

G3 G3 G4 G4

p14

sp3

p24

sp7p24

sp7

pl12

�

GARP

�

1H

Gz

� �

��1

y y�rec

d4 d4 d24 d24

G1G2

d0 d0

p28

15N

13C

d4 d4

p14

sp3

hsqcetgpsisp.2

p24

sp7

p24

sp7

p14

sp3pl12

�GARP

�

1H

1H

GzGz

� �

��1�1

y y�rec�rec

d4 d4 d24 d24

G1

G2

d0 d0

p28

15N

15N

13C

13C

d4 d4

hsqcetgpsi

�

GARP

�

1H

1H

GzGz

� �

��1�1

y y�rec�rec

d4 d4 d24 d24

G1

G2

d0 d0

p28

15N

15N

13C

13C

d4 d4

hsqcetgpsi2

G3 G3 G4 G4

�

GARP

�

1H

1H

GzGz

� �

��1�1

y y�rec�rec

d4 d4 d24 d24

G1

G2

d0 d0

p28

15N

15N

13C

13C

d4 d4

hsqcetgpsisp

p14

sp3

p14

sp3

p14

sp3

p14

sp3pl12

pl12

pl12

optional

optional

optional



NMRGuide4.0

d1

d1

d1

d1

d1

d1

�

GARP

�

1H

1H

GzGz

� �

��1�1

y y�rec�rec

d4 d4 d24 d24

G1

G2

d0 d0

p28

15N

15N

13C

13C

d4 d4

hsqcetgpsisp2

p14

sp3

p14

sp3

G3 G3 G4 G4

p14

sp3

p14

sp3

pl12

optional

�

GARP

�

1H

1H

GzGz

� �

��1�1

y y�rec�rec

d4 d4 d24 d24

G1

G2

d0 d0

p28

13C

13C

d4 d4

hsqcetgpsisp2.2

p14

sp3

p14

sp3

G3 G3 G4 G4

p14

sp3

p14

sp3

p24

sp7

p24

sp7p24

sp7

p24

sp7

pl12

�

GARP

�

1H

1H

GzGz

� �

��1�1

y y�rec�rec

d4 d4 d24 d24

G1G2

d0 d0

p28

15N

15N

13C

13C

d4 d4

p14

sp3

p14

sp3

hsqcetgpsisp.2

p24

sp7

p24

sp7

p24

sp7

p24

sp7

p14

sp3

p14

sp3pl12


�GARP

�

1H

Gz

� �

��1

y y�rec

d4 d4 d24 d24

G1

G2

d0 d0

p28

15N

13C

d4 d4

hsqcetgpsi

�

GARP

�

1H

Gz

� �

��1

y y�rec

d4 d4 d24 d24

G1

G2

d0 d0

p28

15N

13C

d4 d4

hsqcetgpsi2

G3 G3 G4 G4

�

GARP

�

1H

Gz

� �

��1

y y�rec

d4 d4 d24 d24

G1

G2

d0 d0

p28

15N

13C

d4 d4

hsqcetgpsisp

p14

sp3

p14

sp3pl12

pl12

pl12

optional

optional

optional


NMRGuide4.0

d1

d1

d1

d1

d1

d1

�GARP

�

1H

Gz

� �

��1

y y�rec

hsqcetf3gpsi

d26

G1 G3

d0 d0

p28

15N

13C

d26 d24 d24 d26 d26�

G2

p14

sp3

pl16

optional

�GARP

�

1H

Gz

� �

��1

y y�rec

hsqcetf3gpsi2

d26

G1 G3

d0 d0

p28

15N

13C

d26 d24 d24 d26 d26�

G2

p14

sp3

G5 G5G4 G4

pl16

optional10 9 8 7 6 5 4 3 2 1 0 ppm


hsqcetf3gp

�

GARP

�

1H

1H

�1�1

y

�rec�recd26

d0 d0

GzGz

G3

15N

15N

13C

13C

��

1H

1H

�1�1

y

�rec�rec

d0 d0

p28

GzGz

G1

15N

15N

13C

13C

d26 d26 d26�

p14

sp3

p14

sp3

G2

pl16

optional

ppm

6.06.57.07.58.08.59.09.510.0 ppm

102

104

106

108

110

112

114

116

118

120

122

124

126

128

1JNH

C C C N C C N

H H HH

C

H H

156


NMRGuide4.0

d1

d1

d1


�GARP

�

1H

1H

GzGz

� �

��1�1

y y�rec�rec

hsqcetf3gpsi

d26

G1 G3

d0 d0

p28

15N

15N

13C

13C

d26 d24 d24 d26 d26�

G2

p14

sp3

p14

sp3

pl16

optional

�GARP

�

1H

1H

GzGz

� �

��1�1

y y�rec�rec

hsqcetf3gpsi2

d26

G1 G3

d0 d0

p28

15N

15N

13C

13C

d26 d24 d24 d26 d26�

G2

p14

sp3

p14

sp3

G5 G5G4 G4

pl16

optional

157

hsqcetf3gp

�

GARP

�

1H

�1

y

�recd26

d0 d0

Gz

G3

15N

13C

��

1H

�1

y

�rec

d0 d0

p28

Gz

G1

15N

13C

d26 d26 d26�

p14

sp3

G2

pl16

optional


NMRGuide4.0

d1

d1

d1

hsqcetfpf3gp

�

GARP

�

1H

�1

y

�recd26

d0 d0

Gz

G3

15N �

GARP

�

1H

�1

y

�rec

d0 d0

Gz

G1

15N

d26

d26 d26

�

G2

�

G4 G5

-x-x

hsqcfpf3gpphwg

�GARP

1H

�1

y

�recd26

d0 d0

Gz

G3

15N

GARP

1H

�1

y

�rec

d0 d0

Gz

G1

15N

d26

d26 d26

�

G2

�

-x-x

G3

-x

13C

13C

p11

sp1

-x

optional

pl16

pl16

�

1H

Gz

�1

y

�rec

G3

fhsqcf3gpph

d26

15N

G3

d26

G2

p14

sp3

G2

13C

�

G1 G1

d26 d26

d0 d0

GARPGARP

pl16

optional

-x

�

1H

Gz

�1

y�rec

G3

hsqcf3gpph19

d26

15N

G3

d26

G2

�

G1

d26 d26p28

d0 d0

GARPGARP

pl16

p29

sp11

p11

sp1

p29

sp11

p8

sp13


�

GARP

�

1H

1H

GzGz

� �

��1�1

y y�rec�rec

hsqcetfpf3gpsi2

d26

G1 G3

d0 d015N

15N

13C

13C

d26 d24 d24 d26 d26�

G2

p8

sp13

p8

sp13

�

GARP

�

1H

1H

GzGz

� �

��1�1

y y�rec�rec

hsqcetfpf3gpsi

d26

G1 G3

d0 d015N

15N

13C

13C

d26 d24 d24 d26 d26�

G2

p8

sp13

p8

sp13

G5 G5G4 G4

pl16

pl16

optional

optional

158


NMRGuide4.0

d1

d1

d1

d1

d1

d1

hsqcetfpf3gp

�

GARP

�

1H

1H

�1�1

y

�rec�recd26

d0 d0

GzGz

G3

15N

15N �

GARP

�

1H

1H

�1�1

y

�rec�rec

d0 d0

GzGz

G1

15N

15N

d26

d26 d26

�

G2

�

G4 G5

-x-x

hsqcfpf3gpphwg

�GARP

1H

1H

�1�1

y

�rec�recd26

d0 d0

GzGz

G3

15N

15N

GARP

1H

1H

�1�1

y

�rec�rec

d0 d0

GzGz

G1

15N

15N

d26

d26 d26

�

G2

�

-x-x

G3

-x

13C

13C

13C

13C

p11

sp1

p11

sp1

-x

optional


pl16

pl16

�

1H

1H

GzGz

�1�1

y

�rec�rec

G3

fhsqcf3gpph

d26

15N

15N

G3

d26

G2

p14

sp3

p14

sp3

G2

13C

13C

�

G1 G1

d26 d26

d0 d0

GARPGARP

pl16

optional

-x

�

1H

1H

GzGz

�1�1

y�rec�rec

G3

hsqcf3gpph19

d26

15N

15N

G3

d26

G2

�

G1

d26 d26p28

d0 d0

GARPGARP

pl16

159

p29

sp11

p29

sp11

p11

sp1

p11

sp1

p29

sp11

p29

sp11

p8

sp13

p8

sp13

�

GARP

�

1H

Gz

� �

��1

y y�rec

hsqcetfpf3gpsi2

d26

G1 G3

d0 d015N

13C

d26 d24 d24 d26 d26�

G2

p8

sp13

�

GARP

�

1H

Gz

� �

��1

y y�rec

hsqcetfpf3gpsi

d26

G1 G3

d0 d015N

13C

d26 d24 d24 d26 d26�

G2

p8

sp13

G5 G5G4 G4

pl16

pl16

optional

optional


NMRGuide4.0

d1

d1

d1

d1

d1

d1

hsqcetf3gpxy

�

GARP

�

1H

1H

�1�1

y

�rec�recd1

d0 d0

GzGz

G3

15N

15N

13C

13C

��

1H

1H

�1�1

y

�rec�rec

d0 d0

GzGz

G1

15N

15N

13C

13C

d26 d26 d26�

p14

sp3

p14

sp3

G2

pl16

optional

d26 ��

xy16

xy16

xy16

xy16

loop loop

hsqcetf3gpxy.2

�

GARP

�

1H

1H

�1�1

y

�rec�recd1

d0 d0

GzGz

G3

15N

15N

13C

13C

��

1H

1H

�1�1

y

�rec�rec

d0 d0

GzGz

G1

15N

15N

13C

13C

d26 d26 d26�

p14

sp3

p14

sp3

G2

pl16

optional

d26 ��

xy16

xy16

xy16

xy16

loop loop

G2

�

G1



NMRGuide4.0

bruker pulse program catalogue - ciencias.ula.ve · d1 presat off-reson. d1 presat pl9 d0 d0 pl12...

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