“Early Stage and Demonstration trial results - Quillaja saponaria extract, effective against Botrytis cinerea in table grapes as an

integrated solution for grower´s Botrytis Management Program”

Paulina Sepulveda, MSc – INIA ChileABIM 2013

-Research Collaboration-

Agenda

• Chile in the World• Exports of Table Grapes• Disease: Grey Mold caused by Botrytis cinerea• Available Control Solutions• Our Solution• Experimental and Demostration trials • Summary

Field trials 2011-2012Botristop

Thompson Seedless GrapesAGRÍCOLA LONQUÉN

ISLA DE MAIPO, CHILE

Field trials 2011-2012

Early Stage

Treatment Products Dose Botristop Quillay extract (BS) 2L-HectareIntegrated Boscalid-Botristop-Fenexamid- Botristop-

Botristoplabel

Grower Boscalid- Fenexamid-Citric extract-Fenexamid label

Control -----

•Randomized Blocks•5 Treatments (4 plants)•3 Replications.

Early Stage

•5 sprays every 10 days•From veraison to harvest.

T1

T5

T2

T4

T3

T4

T2

T3

T1

T5

T1

T4

T5

T2

T3

• 25 bunches per plant• Incidence (%), • Severity (Scale 0-4) • Disease index• Phytotoxicity

Evaluation Methodology

Pre harvest

Incidence of grey mold

05-03-2012:

Treatments

Perc

enta

ge

cc

ab

a

Disease index of grey mold

05-03-2012:Di

seas

e in

dex

Treatments

b

ab

a

b

Field Trials 2012-2013

Demonstration

Thompson Seedless GrapesAGRÍCOLA LONQUÉN

ISLA DE MAIPO, CHILE

Demonstration

Treatment

Spraying machinery800L/hectare

Field test size:1 hectare

Data sampling:

H9 H13

BS400Control

H14 H23H18

Grower

H19

BS200

H29 H33H28

integrated

H24

Treatment Products Dose

Botristop Quillay extract (BS) 2L/Hectare

Integrated Boscalid–Botristop- Fenexamid-Botristop- Fenexamid-Botristop

label

Grower Boscalid- Fenexamid—Fenexamid- Citrus extract

label

Control No applications

4-6 sprays every 10 days from veraison to harvest

Evaluation Methodology

• 75 bunches per treatment• Incidence (%), • Severity (Scale 0-4) • Disease index• Toxicity

1- Pre harvest 2- Harvest

• Total weight of infected bunches

Incidence of grey mold

Incidence %, 27/02/2013

bb

a

b

Incidence of grey mold

Incidence %, 27/02/2013

a

b

a

98,25%

Infected grapes

  Control Botristop Integrated Growerkg/plant 1,838 0,705 0,486 0,534

Fit pre harvest result

Avg. kilograms of damaged grapes per plant

Pos

Post HarvestEvaluation

• SO2• Stored fruit at 0°C (30-45 days)• Evaluation of infected berries

Post Harvest Results

Grapes stored at 0°C

Summary• Quillay extract (BS) represents a new alternative

to control Botrytis cinerea.

• BS effectively decreased the incidence of Botrytis cinerea when sprayed alone or alternated with chemical fungicides.

• BS did not caused phytotoxicity in any of the doses evaluated.

• Quillay production platform addresses consistency challenges of formulation´s active compounds.

“Early Stage and Demonstration trial results - Quillaja saponaria extract effective against Botrytis cinerea in table grapes as an

integrated solution for grower´s Botrytis Management Program”

-Thank You-

V