biopsy techniques for children with cancer
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BIOPSY TECHNIQUES FOR BIOPSY TECHNIQUES FOR CHILDREN WITH CANCERCHILDREN WITH CANCER
B.A.JABOB.A.JABO
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OUT LINEOUT LINE
• INTRODUCTION• PREPARATION• TECHNIQUES• TYPES• CONCLUSIONS
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IntroductionIntroduction
• In the past, malignant conditions in children were primarily diagnosed by histopathologic techniques performed on specimens obtained during surgery and involved extirpation of a mass or open biopsy.
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contcont
• Improvements in diagnostic imaging, newer techniques in molecular biology, and progress n surgical techniques have permitted accurate diagnosis and staging of many types of childhood tumors using minimally invasive approaches.
• Simple hybridization strategies & southern blotting were originally used.
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contcont
• Fluorescence insitu hybridization (FISH), polymerase chain reaction (PCR) and reverse transcriptase.
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PreparationPreparation
• Consent• Haematological investigations• Biochemical• Bowel
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TechniquesTechniques
• Open• Percutaneous• Endoscopy• Laparoscopy• Thoracoscopy
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TypesTypes
• Incisional• Excisional• Percutaneous (FNAC, TRUCUT)• Frozen sections
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ConclusionConclusion
• Important – diagnosis & prognosis• Management plan• Multi displinary team work• Long term follow up
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