Illumina’s TruSight Tumor 170 is an enrichment-based, targeted panel NGS assay, optimized to detect 170 gene variants from

common solid tumors. It simultaneously analyzes DNA and RNA extracted from formalin-fixed, paraffin-embedded (FFPE) cancer

tissue samples or from cell lines. The method is automated on the Biomek NXP Span-8 Liquid Handler and has been Illumina

Qualified. The Biomek automated TruSight Tumor 170 workflow (Fig. 1) aligns with the kit’s protocol and guarantees at least 16 RNA

libraries and 16 DNA libraries can be prepared from a single reagent kit. It reduces hands-on time (Tables 1 and 2) while simplifying

library prep workflow. The method interface makes setup easy: use the Method Options Selector (Fig. 2) to choose run options

(e.g., number of samples), and Guided Labware Setup (Fig. 3) with automatic reagent calculation for accurate system prep that

virtually eliminates setup errors.

Biomek NXP Span-8 Automated TruSight Tumor 170

• Simultaneously process up to 32 samples (16 RNA and 16 DNA)

• Flexibility to select modules to run and number of samples to process (3 – 32)

• Modular process preserves reagents and automates most laborious steps

• Proven to deliver quality sequencing libraries revealing known variants1

Biomek Automated TruSight Tumor 170 Assay - Illumina Qualified

40-85ng of RNA

Day 1

Day 2

Key

cDNA Synthesisand Cleanup

A-Tailing, Adapter Ligation,

and CleanupIndex PCR First

Hybridization

First CaptureSecond Capture

and Libraies Amplification Setup

SecondHybridization

Cleanup AmplifiedLibraries

40-120ng of Sheared gDNA

NormalizeLibraries

Prepare forSequencing

Mamual Process Automated Process

Quantify Libraries Start/Stop Point Instrument

Setup

Figure 1. Automated Illumina TruSight Tumor 170 workflow

Modular workflow enables flexible throughput and scheduling of your day

Table 1. Day 1 Time Estimates

Day 18 RNA and 8 DNA 16 RNA and 16 DNA

Offline Time Instrument Time Offline Time Instrument Time

Denature and Anneal RNA, First Strand Synthesis, Second Strand cDNA Synthesis, and cDNA Cleanup 15 mins 2 hr 25 min 2 hr, 15 min

DNA Input Preparation and Fragmentation (Manual) *1 hr, 30 min - 2 hr -

End Repair and A-Tailing, Ligate Adapters, and Clean Up Ligation *15 min 2 hr, 40 min *15 min 3 hr

Indexing PCR (Manual) 45 min - 50 min -

First Hybridization (Manual) *Overnight - *Overnight -

Day 1 Offline Time 1 hr 1hr, 15 min

Day 1 Instrument Time 4 hr, 40 min 5 hr, 15 min

Day 1 Total Time 5 hr, 40 min 6 hr, 30 min

* Steps that can be run in parallel with other on-going processes are not included in total time count.

Table 2. Day 2 Time Estimates

Day 28 RNA and 8 DNA 16 RNA and 16 DNA

Offline Time Instrument Time Offline Time Instrument Time

First Capture 15 min 1hr, 45 min 15 min 2 hr

Second Hybridization (Manual) Min: 1 hr, 30*Max: 4 hr - Min: 1 hr, 30

*Max: 4 hr -

Second Capture and Amplify Enriched Libraries Set Up *15 min 1 hr, 20 min *15 min 1 hr, 35 min

Amplify Enriched Libraries (Manual) 50 min - 50 min -

Clean Up Amplified Libraries *15 min 40 min *15 min 55 min

Library Quantification *20 min - *20 min -

Normalize Libraries *15 min 45 min *15 min 1 hr

Sequencing Set Up (Manual) 20 min - 25 min -

Day 2 Offline Time 2 hr, 55 min 3 hr

Day 2 Instrument Time 4 hr, 30 min 5 hr, 30 min

Day 2 Total Time 7 hr, 25 min 8hr, 30 min

* Steps that can be run in parallel with other on-going processes are not included in total time count.

Contact Beckman Coulter at 1-800-742-2345Learn more

Biomek Automated Workstations are not intended or validated for use in the diagnosis of disease or other conditions

© 2017 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks used herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com

AAG-3266FLY12.17

Discovery In Motion | 3

Compact Bench-Top System

Core Components:

Beckman

Biomek NXP Span 8 TruSight Tumor 170 Sample Prep System

Description

BIOMEK NXP SPAN 8 WITH GRIPPER

BIOMEK NXP HALF TRASH ALP KIT

KIT, 1 ML SYRINGE - 8 PACK

Low Volume Disposable Mandrel Kit, 8-Pack

Biomek Software 4.1

NXP 3X3 ALP

BIOMEK NXP SPAN 8 ACTIVE WASH ALP KT

ORBITAL SHAKER ALP, Large Diameter Orbital Shaker Pins

Static Peltier

3rd Party Hardware

Inheco 3200376 Adapter C ABgene AB-0765, AB-0859 (PN 3200376)

Alpaqua Magnum™ EX Universal Magnet Plate (PN A000380)

Illumina

TruSight Tumor 170 Kit, for Use with NextSeq 500/550 (OP-101-1003, OP-101-1004)

Simple Setup, Flexible Run-Time Options

Figure 2. Automated TruSight Tumor 170 Method Options Selector (MOS). Provides the flexibility to select runtime options with built-in Illumina defined safe stop points.

Figure 3. Automated TruSight Tumor 170 Guided Labware Setup Interface (GLS) Reduces Setup Errors by Guiding the User through the Instrument and Reagent Setup.

Figure 4. Guided Labware Setup includes a reagent calculator with instructions for preparation and placement of each on the system.

Simple Setup Reduces Setup Errors

Contact Beckman Coulter at 1-800-742-2345Learn more

Biomek Automated Workstations are not intended or validated for use in the diagnosis of disease or other conditions

© 2017 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks used herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com

AAG-3266FLY12.17