biology 2250 microbial growth and its control. biology 2250 part 1. microbial growth
TRANSCRIPT
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BIOLOGY 2250
MICROBIAL GROWTH and ITS CONTROL
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BIOLOGY 2250
PART 1. MICROBIAL GROWTH
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Requirements for Growth
Physical Requirements – Temperature, pH, Osmotic PressureChemical Requirements – Carbon, Nitrogen, Sulfur, Phosphorous, Trace Elements, Oxygen, Organic Growth Factors
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Temperature Requirements
Mesophiles
Psychrophiles
Psychotrophs
Thermophiles
Hyperthermophiles
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TEMPERATURE OF FOODS
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OSMOTIC PRESSURE
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OXYGEN REQUIREMENTS
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BINARY FISSION
Little increase in sizeGrowth in numbers to form colonies on an agar plate
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How about these numbers!
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BACTERIAL GROWTH
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GROWTH PHASES
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BIOLOGY 2250
PART 2. CONTROL OF MICROBES
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TERMINOLOGY
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MICROBIAL DEATH
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Types of Cell Damage
Alterations of Plasma Membrane
Damage to Proteins and Nucleic Acids
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PHYSICAL METHODS OF CONTROL
Moist heat
Dry heat
Filtration
High pressure
Desiccation
Osmotic pressure
radiation
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Moist Heat
Autoclave
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Principle of Autoclave
Adjust your recipe to your altitude!
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Dry Heat
Oven
Bunsen burner flame
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FILTRATION
Membrane filters have different pore sizes, e.g. 0.45 µ’s
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High Pressures
Fruit Juices
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Desicattion
Freeze Dried Foods
Dried Meats
Dried Fruits
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Osmotic Pressure
Salting –
Pickling -
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RADIATION
Ionizing – cobalt, gamma rays
Nonionizing – UV light
Microwave
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CHEMICAL CONTROL
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CHEMICAL CONTROL