biolab report
TRANSCRIPT
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EXERCISE 8
Cellular Respiration
August 31, 2010
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CAPUNO, Bingle MariangiolaMATEO, Femina Margaret
MENDOZA, Edgardo Jr.NUEVAS, Jan Patrice
PESIGAN, Jane MeirialSISCAR, Justine Alyssa
Group 3TFCD1
Prof. Sedricke lapuz
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Table of Contents
I. Introduction
II. Methodology
III.Results and Discussions
IV.Conclusion
Bibliography
Select one
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I. INTRODUCTION
Respiration is the breaking down of stored materials found in the body to yield energy. It is a property of all living things. Respiration often occurs in plants during winter or at times when photosynthesis is not possible.
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The process of respiration breaks down stored material such as glucose into ATP which is a readily usable source of energy. For this metabolic process to occur, substances such as oxygen and many other enzymes are necessary that is why the rate of respiration greatly depends on the factors affecting enzyme activation.
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The study aims to show the processes happening in cellular respiration and the different factors that affect the process.
The study also wants to explain the presence of oxygen as a requirement in aerobic respiration and in seed germination. Also, the study discusses the reason why oxygen is a factor in root growth. Another purpose of the study is to figure out the products of respiration and the enzymes involved in the said production.
OBJECTIVES
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SIGNIFICANCE OF THE STUDY
BACK
The study was done in order to find out how oxygen becomes an important factor in cellular respiration and what products can cellular respiration give. In the process, the exercise will reveal how certain enzymes are required for the process to take place. Generally, this exercise will explain the importance of respiration in our daily lives.
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A. Oxygen as a requirement in aerobic
respiration and seed germination
II. METHODOLOGY
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Approx. equal amounts of soaked corn/mongo seeds were placed in
cheesecloth leaving some space for germination.
Two bags of the seeds were suspended in two wide mouthed bottles – Bottle A
containing 20ml of water and Bottle B containing 20ml of pyrogallate solution.
The bottles were tightly covered and were placed in a cool place.
Results were taken after a week of observation.
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B. Oxygen as a factor in root growth
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Two similar cuttings of mayana were put in separate bottles containing equal
amounts of water.
Bottle A had an aerator while Bottle B had none.
Root growth was the observed after a week.
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C. Carbon dioxide as a product of respiration
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First set-up: Soaked corn/mongo seeds were placed in a 125ml flask connected to another flask containing 50ml
Barium hydroxide mixture, by rubber tubing and was tightly sealed.
Second set-up: An amount of 50ml Barium hydroxide mixture was put in one flask connected to another flask containing
KOH solution, by rubber tubing and was tightly sealed.
The set-ups were put in a cool place.
Results were taken after days of observation.
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D. Oxidases & Peroxidases:Some enzymes involved in
metabolism
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The fruit (apple) was peeled and was cut into 15 strips.
The set-ups were observed after 30 minutes and results were taken.
There were five set-ups, each utilizing 3 strips of the fruit.A – The fruit strips were placed in an open petri plate (no
solution).B – The fruit strips were immersed in !% solution of
ascorbic acid.C – The fruit strips were boiled in distilled water for 3
min, and were then set aside.D – The fruit strips were put in distilled water.E – The fruit strips were put in 3% hydrogen peroxide
solution.
BACK
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III. RESULTS AND DISCUSSIONS
A. Oxygen as a requirement in aerobic respiration and seed germination
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Fig. 3.1.1 Mongo seeds, soaked overnight
After one week
Fig 3.1.2 Bottle A- twenty seeds inside the cheesecloth suspended over 20 ml of waterBottle B- twenty seeds inside the cheesecloth suspended over 20 ml of pyrogallol
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Fig 3.1.3 Bottle A(water)- Sixteen out of twenty seeds germinated (80%)
Fig 3.1.4 Bottle B (pyrogallol)- Twelve out of twenty seeds germinated (60%)
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B. Oxygen as a factor in root growth
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Fig 3.2.1 Set-up of experiment
After one week
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Fig 3.2.2 Mayana
cuttings with aerator has more roots
Fig. 3.2.3 Mayana cuttings without aerator
has less rootsNEXT
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C. Carbon dioxide as a product of respiration
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Fig.3.3.1Set-up 1:Flask 1 - mongo seedsFlask 2 - Barium hydroxide solutionSet-up 2:Flask 1: Potassium hydroxide solutionFlask 2: Barium hydroxide solution
After one week
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Fig. 3.3.2 Seeds and Barium Hydroxide- white precipitates were present
Fig. 3.3.3 Potassium hydroxide and Barium hydroxide- No visible changes were observed.
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D. Oxidases & Peroxidases:
Some enzymes involved in metabolism
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Fig. 3.4.1 Exposed in open air
Discoloration- brown
Fig. 3.4.2 Immersed in 1% ascorbic acid solution
No discoloration
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Fig. 3.4.3 Immersed in distilled water then boiled for 3 minutes
No discoloration
Fig. 3.4.4 Immersed in distilled water
No discoloration
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Fig. 3.4.5 Immersed in 3% hydrogen peroxide solution
Discoloration- brown
BACK
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IV. CONCLUSIONFrom the results of the
experiments conducted, it can therefore be concluded that oxygen is a need for the seeds to germinate and to perform aerobic respiration, oxygen is and important growth factor thus, triggers greater growth of roots, seeds emit carbon dioxide as a product of respiration and oxidases and peroxidases prevent oxidation which causes discoloration of fruits and vegetables.
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BibliographyMauseth, J.D., (2009) Botany: An Introduction
to Plant Biology (4th Ed.). Massachusetts: Jones and Barlett Publishers, LLC
Simpson, M.G., (2006) Plant Systematics. USA: Elsevier Academic Press
Internet Sources◦ http://jeq.scijourals.org◦ http://www.chm.bris.ac.uk◦ http://adsabs.harvard.edu
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THANK YOU.