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Biol 1020 Lab 2 - Biochemistry Lab Review

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Page 1: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Biol 1020Lab 2 - BiochemistryLab Review

Page 2: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 1 Which of the following would be found

in starch?

2 3

4

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H HHO

H

2 3

4

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

HO

H

A B

Page 3: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 1

Which of the following would be found in starch?

2 3

4

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H HHO

H

2 3

4

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

HO

H

A B

This is Glucose

Page 4: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 2 If the 2 glucose's below were to join

where and how would the bond form?

2 3

4

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

HO

H

2 3

4

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

HO

H

Page 5: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 2 The bond would form between the 1st

and 4th carbon. It would involve the removal of an OH and a H.

2 3

4

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

HO

HA

2 3

4

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

HO

H

Page 6: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 2 The bond would form between the 1st

and 4th carbon. It would involve the removal of an OH and a H.

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

O

H

A

3

4

6

1

CH OH2

O

H

H

OH

OH

H

H

HO

H

A new 1-4 bond

Page 7: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 3 What is this type of reaction called?

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

O

H

A

3

4

6

1

CH OH2

O

H

H

OH

OH

H

H

HO

H

A new 1-4 bond

Page 8: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 3 The reaction is a condensation reaction and

involves the removal of water (H O).

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

O

H

A

3

4

6

1

CH OH2

O

H

H

OH

OH

H

H

HO

H

A new 1-4 bond

2

Page 9: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 4 Is glucose in this form a reducing

sugar?

2 3

4

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

HO

H

Page 10: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 4 No- The double bonded Oxygen is part of

the ring. We need to heat the solution to open the ring for the Benedicts test to be positive.

2 3

4

5

6

1

CH OH

OH

2

O

H

H

OH

OH

H

H

HO

H

Page 11: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 5

Which of the Catechol temperatures reacted fastest?

A. Ice bath

B. Room Temp

C. 100ºC

D. 40ºC

Page 12: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 5

Which of the Catechol temperatures reacted fastest?

A. Ice bath

B. Room Temp

C. 100ºC

D. 40ºC

Page 13: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 6 Why did the 100ºC Catechol not react?

A.The catechol bonds were broken by the heat

B.The catachol oxidase bonds were broken by the heat

C. The catachol oxidase was denatured by the heat

D. The catechol was denatured by the heat

Page 14: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 6 Why did the 100ºC Catechol not react?

A.The catechol bonds were broken by the heat

B.The catachol oxidase bonds were broken by the heat

C. The catachol oxidase was denatured by the heat

D. The catechol was denatured by the heat

Page 15: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 7 Why did the 0ºC Catechol not react?

A.The catechol was destroyed by the ice

B.There was not enough energy for the reaction to occur

C. The potato was frozen

D. The catechol oxidase was destroyed by the ice

Page 16: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 7 Why did the 0ºC Catechol not react?

A.The catechol was destroyed by the ice

B.There was not enough energy for the reaction to occur

C. The potato was frozen

D. The catechol oxidase was destroyed by the ice

Page 17: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 8 Which of the following would you think are

non- polar molecules based on the ability to mix with oil?

A. Ether

B. Water

C. Soap

Page 18: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 8 Which of the following would you think are

non-polar molecules based on the ability to mix with oil?

A. EtherB. Water

C. Soap

The soap did not mix-it emulsified

Page 19: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 9 Which of the following is a difference between

a fat and a phospholipid?

A. fats have 3 fatty acids and phospholipids have 2

B. fats have non polar heads and phospholipids have polar heads

C. fats are used as an energy store while phospholipids form bilayers (membranes)

D. fats usually contain only H,C,O while phospholipid also contain N and P

Page 20: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Question 9 Which of the following is a difference between a

fat and a phospholipid?

A. fats have 3 fatty acids and phospholipids have 2

B. fats have non polar heads and phospholipids have polar heads

C. fats are used as an energy store while phospholipids form bilayers (membranes)

D. fats usually contain only H,C,O while phospholipid also contain N and P

All are true

Page 21: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Lab 2 - Biochemistry

Your lab bench must be cleaned up before you leave.Place all of the test tubes back in the rack!Leave all material in its original location

Page 22: Biol 1020 Lab 2 - Biochemistry Lab Review. Question 1 n Which of the following would be found in starch? A B

Biol 1020Lab 2 - BiochemistryLab Review