bioinf2bio oportunidades
DESCRIPTION
Sessão de Apresentação do Mestrado em Bioinformática e Biologia Computacional, dia 6 de Maio de 2014 às 16h30 no Anfiteatro da FFCULTRANSCRIPT
“The role of Bioinformatics in basic and applied research”
Carla Oliveira Expression Regulation in Cancer Group, IPATIMUP Medical Faculty of Porto University Porto, Portugal E-mail: [email protected] CEO Bioinf2Bio (www.frombioinformatics2biology.com)
Sessão de Apresentação do Mestrado em Bioinformática e Biologia Computacional Faculdade de Ciências da Universidade de Lisboa Lisbon, Portugal May 6th, 2014 Invitation by: Francisco Couto
Genomics Analysis of human genes Epigenomics DNA and histone-protein modifications Transcriptomics RNA expression Proteomics Protein expression and modifications Metabolomics Low molecular weight substances Toxicomics Toxic substance Cellomics Characteristics and functions of living cells
Ex: ArrayCGH, targeted DNAseq, ExomeSeq, WGS
Ex: miRNA microarray & RNAseq
Bioinformatics biological data storing, retrieving, organizing and analysis
What are OMICs?
Ex: MethylSeq, ChipSeq
Research question > Methodology > Platforms > Analysis
Cell/tissue
RNA
Protein
Metabolites
DNA
DA
TA G
ENER
ATI
ON
(Tb
)
DA
TA
INTE
GR
ATI
ON
Interpretation
VA
LID
ATI
ON
Integrative Approach
Methodology
Metabolomics
Proteomics
Transcriptomics
Genomics
Bioinformatics (Statistics, Pathway Analysis Tools...)
From questions to knowledge
…this knowledge was expected to allow accurate classification
of phenotypes, early detection of disease, discrimination
among disease sub-types, prediction of clinical outcomes,
characterization of disease progression, and personalize
therapeutic approaches. But…
“For every complex problem there is an answer that is clear, simple, and wrong.”
H. L. Mencken
From a health perspective...
How to use OMICs information to address and study specific genes and diseases?
1st Generate a relevant and appropriate scientific question or clinical problem
2nd Study and select the best OMICs strategy
3rd Find the right partners (omics and bioinformatics analysis)
4th Set up reliable bioinformatics analysis and wet lab validation
5th Go back to your original scientific question or clinical problem and fight against fascination
OMICs in practice
Technology: Data Mining and Data Analysis
Knowledge
Data Data Data
Data
Data
Data
Data Data
Question
+
Selection
Analysis
NGS & MICROARRAY DATA ANALYSIS
Bioinf2Bio
miRNA
Microarrays
Expression
Microarrays
Tiling
Microarrays
RNAseq
NGS
Methylseq
NGS
DNAseq
NGS
Cadherin intronic transcription
during mouse embryogenesis (in preparation)
Transcriptome signatures
during TGF-β-induced
EMT and MET (in preparation)
Novel and rare CDH1
intronic variants: relevance
for HDGC (in preparation)
Data mining
ERiC
Bioinf2Bio
techniques
Mistranslation
in/and Cancer (under analysis)
Gastric Cancer Genome &
Transcriptome alterations (sequencing ongoing @ BGI)
NGS & MICROARRAY DATA ANALYSIS
Bioinf2Bio www.frombioinformatics2biology.com
Silver Analysis
Gold Analysis
Platinum Analysis
Bronze Analysis