biohack academy microscopy practicalbiohackacademy.github.io/biofactory/class/2/pdf/5... ·...
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BioHack Academy Microscopy Practical
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Preparing microscope slides
Wiki
Microscope slide
SAMPLE
Microscope cover glasses
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Operating a microscope
Microscope Parts - CC BY SA 4.0
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From a petri dish -Put a small droplet of water on a object slide -Stick the loop into the colony -Swirl the loop through the droplet on the object slide. -Cover the droplet with the cover slide.
From a liquid medium -Take up a small sample with the pipette. -Transfer the droplet onto the object slide. -Cover the droplet with the cover slide.
Under the microscope
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Loeffler Staining
Visualize cell nuclei in eukaryote cells.
Stain: loeffler’s methylene blue solution
CC
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Gram staining
Assess whether the biological sample contains Gram positive or Gram negative strains.
Gram-positive bacteria will be violet color. Gram-negative bacteria will be red or pink.
Stain: Crystal Violet and Safranin
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These slides are published by Waag Society under CC-BY-SA 4.0 license