biochemistry grp 3 copy
TRANSCRIPT
BIOCHEMISTRYLABORATORY
LEADER: Kimberly Faye G. Fainiem
Group 3 : MT14206
SECRETARY: Ma. Jolina S. Ferrer
MEMBERS:Karen Escarez
Eunice ExpectacionVince LakapMariel Angeline Loyola
Pau Laxamana
LIPIDS
4 Categories of large molecules in cells:
CARBOHYDRATES
PROTEINS NUCLEIC ACIDS
Carbohydrates include:
Small sugar molecules in soft drinksLong starch molecules in pasta and
potatoes
CARBOHYDRATES
LIPIDS
Lipids:They are hydrophobic - “WATER FEARING”. Do NOT mix with water.
FAT MOLECULE
PROTEINS
Proteins includes: They are polymers made of
monomers called AMINO ACIDS.
All proteins are made of 20 different amino acids linked in different orders.
Proteins are used to build cells, act as hormones & enzymes, and do much of the work in the cell
NUCLEIC ACIDS
Nucleic Acids includes: Store hereditary information. Contains information for making all the body’s protein.
Two type exist: DNA RNA
EXPERIMENT 1
Experiment 1: Subcellular
ComponentsObjectives: To study the function of any organelle. To isolate it first to its pure form and free of
the contaminants by other organelles.
Experiment 1: Subcellular Components
Schematic DiagramProcedures
1
Experiment 1: Subcellular Components
Schematic DiagramProcedures
2
3
Experiment 1: Subcellular Components
Schematic DiagramProcedures
Experiment 1: Subcellular Components
Data and Results
PRECIPITATE
NUCLEIC ACIDS
PROTEINS
LIPIDS
CARBOHYDRATES
I+++ ++ + +
II+ + +++ +++
III++ +++ ++ ++
NUCLEIC ACIDSDische Reaction (DNA) Diphenylamine (DPA) Reacts to deoxyribose of
DNA (+) blue color
ORCINOL TEST/BIALS
TEST (RNA)Specific test for pentoses (+) blue green color
PROTEINS
Biuret Test CuSO4 – oxidizing agent NaOH - reaction
medium Yields positive results
with polypeptides/ proteins but no with dipeptide
(+) violet color
LIPIDS
Sudan IV Hydrophobic dye 2 methyl phenylazo-beta-
naphtol (+) red color
CARBOHYDRATESMolisch Test Non-specific, general
test for CHO in free and combined form.
(+) purple/ violet color
RESULTS: Precipitate I
Carbohydrates
(-) purple color
Lipids
(-) red color
Proteins
(-) violet color
Nucleic Acids
(+) blue green color +++ ++ + +
RESULTS: Precipitate II
Carbohydrates
Lipids
Proteins
Nucleic Acids
(+) purple color
(+) red color
(-) violet color
(-) blue green color + + +++ +++
RESULTS: Precipitate III
Carbohydrates
Lipids
Proteins
Nucleic Acids
(+) purple color
(+) red color
(+) violet color
(+) blue green color ++ +++ ++ ++
EXPERIMENT 2
Experiment 2:
Cell Membrane (RBC)Objectives: To know the permeability of cell membrane. To know the osmotic pressure, presence of
ions present in the membrane.
Experiment 2: Cell Membrane
Schematic DiagramProcedures
1