biochemistry 412 february 24 th , 2002, lecture biological mass spectrometry
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Biochemistry 412 February 24 th , 2002, Lecture Biological Mass Spectrometry. There are two major types of “soft ionization” methods of mass spectrometry for use in analyzing samples of biological origin: • Matrix-assisted laser desorption • Electrospray. - PowerPoint PPT PresentationTRANSCRIPT
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Biochemistry 412
February 24th, 2002, Lecture
Biological Mass Spectrometry
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There are two major types of “soft ionization” methodsof mass spectrometry for use in analyzingsamples of biological origin:
• Matrix-assisted laser desorption
• Electrospray
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Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry
Mann et al (2001)Annu. Rev. Biochem. 70, 437.
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Attractive Features of Mass Spectrometry
• High resolution (can measure mass differences of ~0.1 Da)
• High sensitivity (10-13 to 10-19[!] moles)
• Ability to work with complex samples (MALDI-MS)
• Ability to interface with other separation techniques (e.g., LC)
• “Reasonably” affordable
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Example of an ESI Mass Spectrum
Griffiths et al (2001)Biochem. J. 355, 545.
Note:mass-charge ratio
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Now for some cool applications
of biological mass spectrometry..!!
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Single Nucleotide Polymorphism (SNP)Genotyping by MS
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Electrospray Ionization (ESI) Mass Spectrometry
Griffiths et al (2001)Biochem. J. 355, 545.
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Solid-State DNA Genotyping
Note: extension reactiondone in thepresence of one or more ddNTPs
Tang et al (1999)
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Tang et al (1999)
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Tang et al (1999)
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Generation of Distance Constraints for Protein Structure Determination Using a Combination of Protein Cross-Links and
Mass Spectrometry
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Young et al (2000)
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Young et al (2000)
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Young et al (2000)
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Hydrogen-Deuterium Exchange and Mass Spectrometry to Probe
Protein Dynamics, Solvent-Accessibility, and Protein-Protein Interactions
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Hamuro et al (2002)
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Hamuro et al (2002)
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Hydrogen - Deuterium Exchange and Protein Conformational Fluctuations
Hoofnagle et al (2003) Annu. Rev.Biophys. Biomol. Struct. 32, 1.
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Hydrogen - Deuterium Exchange: Schematic Experimental Design
Hoofnagle et al (2003) Annu. Rev.Biophys. Biomol. Struct. 32, 1.
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Hamuro et al (2002)
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Hamuro et al (2002)
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Fragment-BasedDrug Discovery
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Erlanson et al (2000)
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Erlanson et al (2000)
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Erlanson et al (2000)
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The Monophore Binds in the Same Relative Orientation, Regardless of Attachment Site
Erlanson et al (2000)
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Erlanson et al (2000)
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Pharmacology UsingImaging Mass Spectrometry
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Chaurand et al (2002)
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