9/8/14 5:13 PM

Lab lecture 1

AWR

Training

ACUC

Sectioning

Optical sectioning

o Change the focus, higher power

o Higher power means smaller depth of field

Light microscope

Need to use electron microscope for smaller one

o Scanning gives surface

o Transmission goes through

Resolution refers to the ability to see distinct points as distinct

points

Diffusion

o Flow from higher to lower concentrations

Rate – mass diffusing in time t.

Proportional to how much surface are it has and

how long it has to go

Prokaryote

o Archeae very different from bacteria

Used bacteria term because people didn’t know what

were archeae

Ex. #8 – prelab viewing angle

All cells have:

o Cell membrane

o Cytosol

o DNA – circular piece of DNA

Genomes are very small compared to eukaryotes

Very efficient in encoding stuff

o Ribosomes – makes proteins

Cell = 1 um cube

pH 7 – how many H are within cell?

Volume?

1 = 1 x 10-4 cm. Thus (1x10-4)3 = 1 x 10-12 = 1 x 10-12mL = 1 x 10-15L

Because 1 cm3 = 1mL =

Ex. 1 um = 1x10-4

Vibrio Isolation

Vibrio Streaking

Different

DNA

The bases are very hydrophobic so they stack on top of each other

Connected by hydrogen bonds

Antiparallel

Anneal primers

o Growth is always from the 3’ end

o If want to make copy of DNA, need to have primer that makes

a new 5’ end and continues to build to a 3’ prime end

o Always start to make a new strand from the old 3’ end

9/8/14 5:13 PM

9/8/14 5:13 PM