bio-luminescence detection project manager: eitan novotny sean dang yu-kun zhang philip tran
Post on 20-Dec-2015
223 views
TRANSCRIPT
![Page 1: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/1.jpg)
Bio-Luminescence Detection
Project Manager: Eitan NovotnySean Dang
Yu-Kun ZhangPhilip Tran
![Page 2: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/2.jpg)
Overview
• Biological rhythms– Samples emit periodic light– Periods around 24 hours– Sample rate once every 10 minutes
• Commercial device flaws– Missing flow through function– Product is costly
• Design goals– Integration with original equipment– Improve function and reduce cost
• MDR Specification• Design Changes
![Page 3: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/3.jpg)
Box Design
• 39cmx39cmx32cm• Designed in Pro
Engineering• Two Pieces
– Main box– Lid (top and front)
![Page 4: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/4.jpg)
Box Design - Details
• Internal temperature must match incubator
• Two 80mm square holes plus one 80mm fan for airflow
• Light isolated environment
• 5 BNC connectors for PMTs and Motor
• 2 18cmx4cm windows for tube baffles
![Page 5: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/5.jpg)
Box Design - Overview
• Light baffle
![Page 6: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/6.jpg)
Main Box
![Page 7: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/7.jpg)
Lid
![Page 8: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/8.jpg)
Motor
• 1240i board works with specific motors
• Parallel motor connection not possible
• Principal needs– Single high torque motor– Four rotating mirrors
![Page 9: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/9.jpg)
Motor - Solution
• Motor is Oriental Motor PK243A1A-SG18– Used in commercial application
• Step up gear used for high precision
• Central gear and outer gear are 5”
• Shafts connect outer gears to mirrors
• Contact switches used for error checking
![Page 10: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/10.jpg)
Light Source/Detector
• Luciferin - emits light• Wavelength range: 268nm
to 537nm• Photomultiplier Tube
(PMT)• Detects wavelength range
from 185nm to 680nm• Effective area 4mm x
20mm
![Page 11: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/11.jpg)
Light Efficiency/Path Length
• Commercial application has a .25 cm path length
• Our light path length is about 3cm to 5cm
• Light goes to the mirror and reflects up to the PMT
• Loss of light due to vertical arrangement
• To minimize loss of light, add mirrors
![Page 12: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/12.jpg)
Light Efficiency - Continued
• Interference from other light sources
• Add reflective walls, to minimize interference from other light sources
![Page 13: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/13.jpg)
Electronics
• H8259 from Hamamatsu
• PCI-6601 Counter Board
• Applied Motion 1240i
• Oriental Stepper Motor
• Computer and software
![Page 14: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/14.jpg)
BudgetPart Cost (each) Qty Price
• H8259 $1150 4 $4600• 1240i $200 1 $200• PCI-6601 $600 1 $600• Motor $177 1 $177• Metal $46.11 6 $277• Gears $62.02 5 $310• Shaft Holder $27.46 5 $137• Shaft $20.05 1 $20• Wires $100 $100
Total* $6421*total cost to date
![Page 15: Bio-Luminescence Detection Project Manager: Eitan Novotny Sean Dang Yu-Kun Zhang Philip Tran](https://reader036.vdocuments.mx/reader036/viewer/2022062320/56649d445503460f94a21ac1/html5/thumbnails/15.jpg)
Conclusion
• MDR Specification done before 2nd semester
• Accomplishments– Gearing design done– Box design done
• Mirror design will be completed before conclusion of semester
• Device should be completed on time
• Device should be under budgetAluminum for box construction