Bio& 241 A&P 1Unit 4 / Lab 1

Histology Slides for Nervous Tissues

• Slides are presented in order of magnification if different view are presented.

• As you view the following slides make sure you can accomplish these goals:

1. Can you identify the tissue observable on the slides?

2. Can you identify the specific structures or layers indicates by the numbered arrows or brackets?

• At the end of a sequence you will find the answers to the above for each slide.

100X

1

1

1

1

22

3

3

400X

3

32

21

4

400X

55

5

5

100X

8

8

8

77

6

8

400X

9

8

7

6

10

11

400X

7

11

10

1212

9

8

Answers to Slides 2 through 7Slide 2: Smear of gray matter low power.Slide 3: Smear of gray matter high power.Slide 4: Longitudinal view of myelinated nerve fibers. Slide 5: X-section of a nerve low power (Masson stain)Slide 6: X-section of a nerve high power (Masson stain)Slide 7: X-section of a nerve high power (H&E stain)

Answers to the numbers:• Multipolar neuron cell body with nissl bodies, nucleus, and

nucleolus.• Nerve fibers (axons of dendrites)• Neuroglial nuclei• Nucleus and nucleolus• Node of Ranvier• Epineurium

Answers to Slides 2 through 7Answers to the numbers:7. Perineurium8. Nerve fasiscle9. Endoneurium10. Myelin sheath11. Nerve fiber12. Nucleus of Schwann cells

100X

4

3

12

5

400X

4

3

2

1

5

100X

7 7

6

6

9

9

8 811

11

10 10

14

13

15

12

16

100X18

17

11

5

400X

20

17

11

5

19

400X

18

11

17

17

17

100X

20

19

5

100X

22

23

21

21

2124

Answers to Slides 10 through 17Slide 10 - 16: X-section of spinal cordSlide 17: Section through Dorsal Root Ganglion

Answers to the numbers:• Dura mater.• Arachnoid mater.• Subarachnoid space.• Pia mater.• X-section through white matter.• Gray matter• Posterior or dorsal white column• Anterior or ventral white column• Lateral white column• Doral gray horn• Ventral gray horn

Answers to Slides 10 through 17Answers to the numbers:12. Anterior or ventral median fissure13. Central canal14. Posterior gray commissure15. Posterior or dorsal median sulcus16. Anterior gray commissure17. Multipolar neuron cell body18. Transverse nerve fibers19. X-section through a nerve fiber20. Myelin sheath created by Oligodendrocytes21. Sensory neuron cell bodies22. Longitudinal view of myelinated nerve fibers 23. Nodes of Ranvier24. Satellite cells