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BIMSTEC International Training Programme on Aquatic Plants Tissue Culture

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V Country Report (Annex 4 - 10)

1. Country Report on Status of aquatic plants in Bangladesh (Annex 4)

2. Country Report on Status of aquatic plants in Bhutan (Annex 5)

3. Country Report on Status of aquatic plants in India (Annex 6)

4. Country Report on Status of aquatic plants in Nepal (Annex 7)

5. Country Report on Status of aquatic plants in Sri Lanka I (Annex 8)

6. Country Report on Status of aquatic plants in Sri Lanka II (Annex 9)

7. Country Report on Status of aquatic plants in Thailand (Annex 10)

Fig. 16 Dr. Apichart Termwichakorn, Freshwater Fish Taxonomy Expert, representative of

director of Inland Fisheries Research and Development Bureau, Department of

Fisheries was a Chairman on country report at Tapeanthong meeting room, Inland

Fisheries Research and Development Bureau, Department of Fisheries.


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VI Study tour

Study visit on the 2 aquatic plant farms namely; White Crane Aquatic Plants Co., Ltd.

in Chacheongsoa province and Aquatic Plant Centre (Thailand) Co., Ltd., in Nakhon

Ratchasima Province.

1. White Crane Aquatic Plants Co., Ltd., is the exporter of aquatic plants for over

30 years with more than 100 species of aquatic plants in 20 acres cultivated area, where

located on the Suwintawong Road, Chacheongsoa province. The White Crane Aquatic Plants

Co., Ltd. has an expertise in aquatic plant for both domestic market and exporting to foreign

countries. The study visit experiences on the aquatic plant farms in Thailand will able to

creative and apply experiences gained form developed aquatic plant propagation in

BIMSTEC member countries.


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Fig. 17

Study visit at White Crane Aquatic Plants Co., Ltd, Chacheongsoa province.

3. Aquatic Plant Centre (Thailand) Co., Ltd. was founded in 1984. Located in

Nakhon Ratchasima Province. There are approximately 30 rai cultivated area with

greenhouse and hydroponics system. The company specializes in breeding of

aquatic plants for decorating aquariums. The production of this farm more than 150

species and variety of aquatic plant both native and exotic species. The Aquatic

Plant Centre (Thailand) Co., Ltd. provides a wide selection of aquatic plants

throughout the year. The company exports to many countries in the global market

including Europe, America , Asia, Africa and Oceania.

Fig. 18 Study visit at Aquatic Plant Centre (Thailand) Co., Ltd., Nakhon Ratchasima Province.


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VII Evaluation The BIMSTEC International Training Programme on Aquatic Plants Tissue

Culture

Personal Information

1. There are seven participants (100%) attend in this training. They consist of

biologist/researcher from Bangladesh, Bhutan, India, Nepal, Sri Lanka and Thailand.

2. Gender: There are 4 Male (57%) and 3 Female ( 43%)

Objective

All participants (100%) indicate that their main motivation, or reason, to attend this

training course is to improve my knowledge and skills for my job, while a few participants

add that they were sent by their employer which they could have opportunity for

international travel

Participants Expectation

1. The most important areas of knowledge and skills that participants expected to

improve/obtain in attending this course are as followings;

Techniques knowledge such as high technique in laboratory, selection of

aquatic plants part for culture techniques and sterilization, culture of plant

part on media under aseptic techniques, the practical techniques in tissue

culture, practical technique of tissue culture, develop skills in tissue culture.

Aquatic plants such as identification of aquatic plants, importance of aquatic

plants, identification of aquatic plants, Botanical basis of tissue culture, other

theoretical aspects, aquatic plant propagation.

Tissue culture such as tissue culture of these plants, media preparation for

plant tissue culture.

Good management practices such as new instrument and machines.

Laboratory design and layout.

Visiting aquatic plants farms.


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2. There are six participants (or 86%) think they were fulfilled of their expectation. Only one

(or 14%) was not. There are some comments regarding on the expectation fulfillment as

the followings:

Expectation almost fulfill but field visit should be more instead of

laboratory/lecture.

To attend this training, I can develop my knowledge on aquatic plant tissue

culture, I have real knowledge in the practical way.

Wish to know background on how the aquatic plant become importance,

what is tissue culture.

all about are fulfill especially preparation of stock solution, preparation of

media and tissue culture practical.

Here, they used high/new technique and machineries/instrument.

Training Duration

1. There are five participants (or 71%) felt that the 30 days training duration appropriate for

them but the rest two (or 29%) said the appropriate duration should be 21-22 days.

2. There are six participants (or 86%) feel that period of training during August-September are

appropriate for them while one participant prefer during March –April.

Course Content

1. Participants comment on subjects by rating as following;(1 = very poor to 10 = very good)

Amount of

Content

Applicability

Overall 9.42 8.95

Lecture 9.42 8.96

Practical 9.52 8.94

Visiting 9.31 8.95

Class & Discussion

BIMSTEC Fisheries Cooperation 8.6 8.5

Potential of Aquatic plants and ornamental fish Industry in

Thailand 8.6 8.3


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Applicabilit

y

Amount of

Content

Introduction to aquatic plants tissue culture 9.6 9.0

The botanical basis for tissue culture 9.3 8.5

Laboratory design and equipment 9.6 9.5

Laboratory operation and management 9.9 9.7

Equipment and materials preparation 9.8 9.8

Aquatic plant tissue culture medium 9.6 8.7

Procedures for preparation of macronutrients stock solutions. 9.7 9.0

Preparation of micronutrients stock solutions 9.9 8.7

Procedures for preparation of plant growth regulators stock

solutions 9.7 8.5

Preparation of vitamins stock solutions 9.6 8.3

preparation of buffer solutions 9.3 8.8

Liquid medium preparation 9.3 8.8

Semi-solid medium preparation 9.3 8.5

Media preparation for Special Purposes Ι 8.6 8.2

Media preparation for Anubias spp. tissue culture 9.6 9.2

Media preparation for Microsorium spp. tissue culture 9.6 8.8

Preparation of explants materials and disinfectants 10.0 9.5

Subculture technique 10.0 9.5

Plant growth regulators in plant tissue culture 9.4 8.8

Explants materials and sterile technique 9.9 9.3

Micropropagation procedures 9.4 8.7

Shoot, callus and embryo culture 9.6 8.8

Tissue culture of selected aquatic plants 9.9 9.3

Callus culture of Hygrophila spp. 9.1 8.8

Meristem culture for virus elimination 9.0 8.5

Plant regeneration via organogenesis and embryogenesis 9.1 8.8

Country report Ι 9.5 9.0

Shoot culture of Anubias spp. 9.7 9.2


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Amount of

Content

Applicability

Micropropagation of Cryptocoryne wendtii 9.7 9.4

In vitro culture of water ferns 9.1 8.8

Biological contaminants 9.4 9.6

Hardening off 9.7 9.4

Country report ΙΙ 9.3 9.0

Acclimatization of planlets 9.3 9.0

Places visited

1) Bung Chawarg Aquarium 9.3 8.8

2) Aquatic Plants Culture Farm I 9.1 8.8

3) Aquatic Plants Culture Farm II 9.5 9.3

2. Some ideas on subjects in this course.

Vegetative propagation in detail ( with different media substrate)

Maintaining of green house

Preparation of hydroponics technique culture media and its

maintaining technique

Identification of aquatic plant should be given priority

3. All seven participants (or 100%) think that there are no any practical works that are not

directly related or should be omitted.

4. There are two participants (or 29%) think that the places to visit arranged in this course

are suitable, one participant (or 14%) do not give comment. But four participants (or 57%)

think that some places to visit are to be added such as;

Aquatic plants farm

Aquatic plants market

Study visit at Phuket

Fish breeding center


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5. All seven participants (or 100%) think that the visiting sites beneficial to them. The

following are their reasons.

It is help us to get idea on how the plant culture and management in

large scale for the export market. We can observe difference packing

and potting methods for export market.

The farm design and layout ; the working techniques that are being

used in designed farm and markets are all, I would say knowledge to

us the visit to different aquaria and aquatic plants, for market has also

benefited us greatly.

Ornamental fish and aquatic plant market are important to get idea

about varieties and market value. Aquatic farm is good for

management practices.

Being directly involved in fisheries sector. Some visit programme

regarding inland fisheries should be included

6. The comment on the total content of the course, there are four participants (or 57%) feel

satisfactory, one (or 14%) do not give comment, while two (or 29%) feel that the course

should be improved. Some aspects to be improved are as follow;

To be improve farm visit

Aquatic plant tissue culture in general has been very much satisfied,

but aquatic plant classification would have helped us even more

Ornamental aquatic plants normally move with ornamental fish

species.

If aqua-scaping that is beneficial.

Training Methods & Equipment and Facilities

1. Rating on training methods used in the course

Lectures: 86% is appropriate, 14% inappropriate (It is too little)

Practical : 100% is appropriate, 0% inappropriate


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Field Visiting 86% is appropriate, 14% inappropriate (It is too little)

2. All seven participants (100%) think the selected training methods are sufficient.

3. All seven participants (100%) think the training equipment and facilities are sufficient.

Living Condition

1. Rating on accommodation [1 = very poor to 10 = very good]

1. Hotel in Bangkok 9.7

2. Hotel in Suphanburi 8.1

3. Hotel in Chonburi 8.0

2. Rating on living condition at the Hotel Bangkok [1= very poor to 10= very good]

1. Bed rooms 9.7

2. Food 9.4

3. Recreation equipment 9.4

4. Showers/toilets 9.3

5. Telephone & TV 3

6. Cleaning , staff, environment 10

3. Rating on relationship

Between instructors, resource persons and participants

86% excellent

14% good

Between course management staff and participants

86% excellent

14% good

Between other APOFRI/DOF staff and the participants


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57% excellent

43% good

Between other FIFAD/DOF staff and the participants

57% excellent

29% good

14% do not give rating

Strengths and Weaknesses of this course

Some strengths and weaknesses of this course that identified by participants.

Strengths such as ;

The full course is arranged in simple way. So it is easier to get knowledge

to newcomer into aquatic plants tissue culture. Also in simple and short

way, all importance things are added into course. This is very

importance.

It is a good course, and very importance for my work

State of art laboratory facilities

Excellent instructors committed skills

Excellent team work, very good coordination of staff member of the

department.

The in-depth knowledge of all the instructors, lecturers, and those

working in the laboratory on different aspects of aquatic plants and this

tissue culture


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This course very helpful for our ornamental fish and plant industry.

Weakness :

Inadequate field visit programme.

Participants give some suggestions for overall course improvement as followings ;

The overall is ok.

More practice lessons should be added.

For more benefit to the country organizer of training and the nominating

countries, the same group of trainees should be called/selected to be trained in

the advance tissue culture of aquatic plants because of some basic lessons

could be omitted.

Participant’s plans to do, after they go back to their country in order to make the

knowledge gained form this training programme to get more benefit to their country

are as the followings;

Course experience share with my colleagues, higher authority. And

recommendation to develop a development project programme (DPP) about

the aquatic tissue culture & aquatic plant classification.

If my office can be established aquatic plant tissue culture laboratory, I can

develop it, and make arrangement of training programme to farmer, university

student. Production can be increased by tissue culturing of high demand

plants.

We have aquatic plants in my office and work to develop there.

I will prepare the report regarding the training which will include importance

of aquatic plants, its present and scope. Also I will cover some areas i.e.

media preparation, equipment necessary for tissue culture etc. and present

and definitely start some works towards it.


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Try to complete the inventory of aquatic plants of the countries. These are

available with various agencies in statute of states.

Encourage the private entrepreneurs to ask my organization for funding

support under innovative schemes programme as NFDB has not funded any

agency, so far on ornamental aquatic plant propagation/ tissue culture

Try to place this proposal in executive council meeting of NFDB to include in

NFDB guideline.

Like many other BIMSTEC member countries, I would explore the prospective

of development and promotion of ornamental aquatic plants. This involves

first our studying, identifying and listing all indigenous aquatic plant in our

country.

First I submit complete report regarding this training programme. Then I will

discuss with both our government institutes and private sector to improve

aquatic plant production by using tissue culture.

Participant’s suggestion on areas cooperation that might be interesting and useful for

BIMSTEC countries;

Hydroponic culture

Visit among BIMSTEC country

Trade

Water quality management

Pond construction and management

Induced fish breeding technique

Feed preparation for fish //live feed culture and //farm management


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Participant’s plan of action in order to support for Network of Ornamental Aquatic

Plant researches, when they are back to their countries are as the followings;

Bangladesh

Bhutan

India

Nepal

I will recommend aquatic plants, identification, the preparation of the project

propagation to country's context and make it fruitful.

I will keep in touch with the members from training and staff of DOF, Thailand to

exchange idea and do some collaboration researches and study.

I will compile the inventory ornamental aquatic plants of all the state.

I will coordinate with scientists of CIFA, CIFE to take up researches with the

funding support from NFDB under technology programme/ innovative schemes.

I will first make approach towards inventory. I will write proposal regarding it and

ask some sort of fund require for inventory taking.

Sri Lanka I

Sri Lanka II

Thailand

I will keep contact with lecturer, staff and trainees to exchange information and

help for solving problems which may arise in the future.

I will arrange public presentation for aquatic plants propagation including tissue

culture.

I will give all information to farmers and officers.

I will keep contact among trainees and exchange information.

Some suggestions by participants for the next Fisheries Cooperation in BIMSTEC are;

Soilless culture for aquatic plants propagation

BIMSTEC trainees get-together

The group of young scientists, have intention to learn and have basic knowledge

in each training course

Cooperation in aquaculture, nutritional security. Fish production through better

management practices (BMP)

Breeding and hatchery management of some selected (importance) fishes

VIII Excursion in Thailand

1. Excursion in Bangkok; Bangkok is the capital, largest urban area and primary city

of Thailand. The study tour in Bangkok including the interesting places are


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Vimanmek Palace, The Grand Palace, Wat Phra Kaew, Wat Phra Chettuphon

Wimon Mangkhalaram Ratchaworamahawihan or Wat Pho.

Fig. 19 Excursion in Bangkok.

2. Excursion in Suphanburi province; Suphan Buri is one of the central provinces of

Thailand, is approximately 107 km North western of the city of Bangkok. Neighboring

provinces are (from north clockwise) Uthai Thani, Chai Nat, Sing Buri, Ang Thong, Phra

Nakhon Si Ayutthaya, Nokhon Pathom and Kanchnaburi. The name of the province literally

means City of Gold. The terrain of the province is mostly low river plains, with small

mountain range in the north and the west of the province. The southeastern part with very

low plain of the Tha Cheen river is paddy rice farming area. The study tour in Suphanburi

province including the interesting places as follow :

2.1 Suphanburi Agricultural Extention and Development Centre (Plant-

tissue culture) located in Tambon Phlapphla Chai. The centre was established to develop

agriculture and plants, such as tissue culture, nursery, and plant distribution. The tissue

culture center at Suphan buri is the most modern in Thailand. At present, the center

produces plant seedlings by tissue culture and gives them to the farmers free of charge.

They can produce 200,000-300,000 seedlings per year.

2.2 Bueng Chawak Aquarium exhibits various species such as of fresh-water

aquarium and marine fish aquarium.

The first building exhibits fresh-water fishes such as Mekong Giant Catfish,

Clown Feather Back Fish, Bony Tongue Fish, Tiger Perch Fish, etc., Aquatic plants species

such as Echinodorus, Rotala, Ammania, etc.


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The second building exhibits marine fishes such as Shark, Ray, Sea bass,

Grouper, Clown fish, Sea dragon, Seahorse, Puffer fish etc.

Freshwater Crocodile Pond landscaped for a natural look, the pond houses

60 Siamese crocodiles of 1.5-4.0 meters.

2.3 Old Sam Chuk Market located along Tha-Chin River : This Chinese

community and old-fashioned market with wooden shop houses remain in Thai original style

a century ago.

2.4 Dragon Descendants Museum is built in a shape of a huge fiberglass

cloud dragon spraying water measuring 135 m long, 35 m high and 18 m wide adjacent to

the city pillar shrine.

Fig. 20 Study visit at Bueng Chawak Aquarium.


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Fig. 21 Excursion in Suphanburi province.

3. Excursion in Chonburi province. This province is one of the Eastern provinces

of Thailand. Neighboring provinces are (from north clockwise) Chachoengsao, Chanthaburi

and Rayong to the west is the Gulf of Thailand. The study tour in Chonburi province

including the interesting places as follow :

Pattaya Floating Market, This amazing Thai style shopping arena covers an

area of some 23 Rai.

Pattaya, located off the Gulf of Thailand, is approximately 145 km south of

the city of Bangkok. Pattaya beach, the half-moon-shaped beach is about 4 kilometers long

with beach road running parallel to the beach.

Khao Kheow Open Zoo, the famous zoo operated by the Zoological Garden

Organization and covers a vast tract of forestland. There are more than 200 species of

wildlife living in their natural habitual. They include both domestic and imported types.


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Fig. 22 Excursion in Chonburi province.


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IX Closing of the BIMSTEC International Training Programme on Aquatic Plants Tissue

Culture.

Mr. Somwang Pimolbut Director of Inland Fisheries Research and Development

Bureau, was a chairman of the closing ceremony, he wished all participants a safe trip back

home and then declared the BIMSTEC International Training Programme on Aquatic Plants

Tissue Culture officially close.

Fig. 23 Officially close by Mr. Somwang Pimolbut Director of Inland Fisheries Research and

Development Bureau, Department of Fisheries, and Mr. Surasak Chursukonthip, Deputy


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Director-General, the Department of International Economic Affairs, at Tapeanthong

meeting room on 26thSeptember2011.

Fig. 23 Mr. Somwang Pimolbut, Director of Inland Fisheries Research and Development

Bureau, presented certificates to the trainees.


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Fig. 24 Remarks by Mr. Surasak Chuasukonthip, Deputy Director-General, Department of

International Economic Affairs at the Closing Ceremony of the BIMSTEC International

Programme on Aquatic Plant Tissue Culture, 26th September 2011 at the Department

of fisheries, Bangkok, Thailand

Fig. 25 Reported by Mr.Sujin Nukwan, Director of Aquatic plants and Ornamentalfish Research

Institute on the closing ceremony of the BIMSTEC international training programme

at Tapeanthong meeting room, Department of Fisheries.

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