beyond lifetime reproductive success: the posthumous reproductive dynamics of trinidadian guppies
DESCRIPTION
Talk on the use of State-Space Capture-Recapture models and Matrix Analysis to understand the dynamics of posthumous reproduction in guppies. Guppy females store male sperm, which means that males can produce offspring after death.TRANSCRIPT
![Page 1: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/1.jpg)
Beyond Life+me Reproduc+ve Success The dynamics of posthumous reproduc+on in Trinidad guppies
Andrés López-‐Sepulcre
![Page 2: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/2.jpg)
Collaborators
General Protocol
•! 3 scales sampled, stored
dry in tubes
•! DNA isolation (enough for
~50 PCRs)
•! Genotype at ~12
microsatellite loci (more available)
•! Parentage assignment
via CERVUS, PAPA, PASOS…
Swanne Gordon Univ. Jyväskylä Finland
Paul Bentzen & Ian Paterson Dalhousie Univ.
Canada
David Reznick Univ. California, Riverside
USA
![Page 3: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/3.jpg)
Sperm Storage in Animals
• Common in many species • Can lead to posthumous reproduc+on • Its dynamics in nature largely unknown • Evolu+onary significance?
![Page 4: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/4.jpg)
Trinidadian guppy, Poecilia re*culata
• Two dis+nct phenotypes associated to preda+on and resources
• Differences in mul+ple traits and ecological effects • Several independent evolu+onary events • Rapid evolu+on aSer transloca+on
![Page 5: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/5.jpg)
Reznick et al. 1990. Nature
Experimental evolu+on in the wild
![Page 6: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/6.jpg)
Guppy Mark Recapture
• All individuals >14mm individually marked • Followed monthly • Gene+c samples for pedigree reconstruc+on
![Page 7: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/7.jpg)
Closed canopy
h2 = 0.30
Capture probabilities
Male Size at Maturity
![Page 8: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/8.jpg)
Evolu+on of male size
• Common garden
Source Popula+on
Derived
![Page 9: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/9.jpg)
Survival
Standard Length (mm)
Sur
viva
l pro
babi
lity
![Page 10: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/10.jpg)
DEADALIVE
DEADALIVE
Reproduc+on • Larger individuals reproduce less
… but we had only counted the live ones • Guppy females store male sperm
• Hot to count in the dead?
Prop
or+o
n of re
cruits sired
Standard Length (mm)
![Page 11: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/11.jpg)
Juvenile (J)
Mature not reproducing
(M)
Mature reproducing
(R)
Dead reproductive
(S)
!J1" µ( )
!M1" #( )
!R
!S
! Jµ1"#
()
!J µ"
!M"
!1"#
M
()$
1!" R
()#
Conception
3 m
onth
s
!R"R#R
!S"S#S
State Space Models
• Define model that defines unobserved biology
Model includes transi+on between states
Mature Not Reproduc+ve
Mature Reproduc+ve
Dead Reproduc+ve Juvenile
![Page 12: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/12.jpg)
State Space Models
• Overlay an observa+on model • Data is a result of the observa+on model condi+onal on the biology model
• We can es+mate ‘invisible’ biological parameters
Alive Alive
Not Rep. Observed
Not Rep Not Obs
Alive Alive Alive Zombie Zombie Dead
Not Rep. Not Obs.
Reproduced Observed
Reproduded Not Obs
Not Rep Not Obs
Not Rep. Not Obs.
![Page 13: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/13.jpg)
Results
●
● ●
●
● ●●
●
01
23
4Months reproductive
1 2 3 4 5 6 7 8 9 10
Live males(a)
●
●
●
● ●●
●●
●
●
● ●01
23
4
Months dead1 2 3 4 5 6 7 8 9 10
Dead males(b)
Indi
vidu
al c
ontri
butio
n to
pop
ulat
ion
recr
uitm
ent (
%)
● ● ● ● ●● ●
●
●●
01
23
4
Months reproductive1 2 3 4 5 6 7 8 9 10
Females(c)
Individu
al con
tribu+
on to
recruitm
ent
…whatever
![Page 14: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/14.jpg)
Results
2 4 6 8 10
050
100
150
Months since introduction
Num
ber o
f mal
es
Dead reproductives (S)Reproductive adults (R)Non−reproductive adults (M)Juveniles (J)
●
●●
●
●
●
●
●
●
●
2 4 6 8 100.
000.
050.
100.
150.
200.
250.
30
Months since introduction
Prop
ortio
n of
new
recr
uits
sired
by
dead
mal
es
![Page 15: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/15.jpg)
Does size macer?
DEADALIVE
DEADALIVE
Prop
or+o
n of re
cruits sired
Standard Length (mm)
Prop
or+o
n of re
cruits sired
Standard Length (mm)
![Page 16: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/16.jpg)
Thoughts
• How does storage affect natural selec+on? – Maintenance of diversity – ‘Bet-‐hedging’
![Page 17: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/17.jpg)
Thoughts
• How does storage affect sexual selec+on? – Opera+onal Sex Ra+o – Male-‐male compe++on
Carotenoid Melanistic
HP
Source
HP
Source Canopy Open
Canopy Open
Pro
po
rtio
n s
ho
win
g c
olo
r
![Page 18: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/18.jpg)
Thanks!
![Page 19: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/19.jpg)
Sensi+vity & Elas+city Analysis
![Page 20: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/20.jpg)
Focal Experiment • Individual based data • Monthly • Phenotype (pictures) • Pedigree and gene+c samples • >15,000 ind, >10 genera+ons • ~90% recapture rates • Ecosystem data • Physical variables • Bimonthly es+mate of compartments • Annual 15N drip
Mesocosms
• Ecological effects
Laboratory
• Common garden phenotyping
Structure of the study
Paul Bentzen, Ian Paterson (Univ. Dalhousie)
![Page 21: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/21.jpg)
Open canopy
h2 = 0.16
Mature male size
![Page 22: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/22.jpg)
Reproduc+on DEAD ALIVE
DEAD ALIVE
Random effects: Groups Name Variance Std.Dev. FishID (Intercept) 2.09198 1.44637 Sampling (Intercept) 0.59734 0.77288 Number of obs: 661, groups: FishID, 101; fSampling, 12Fixed effects: Estimate Std. Error z value Pr(>|z|) (Intercept) -7.97496 4.07333 -1.958 0.0502 .SL 0.01998 0.22781 0.088 0.9301 aliveFALSE 1.26180 3.23465 0.390 0.6965 SL:aliveFALSE 0.02183 0.18019 0.121 0.9036
Random effects: Groups Name Variance Std.Dev. FishID (Intercept) 1.06576 1.03236 fSampling (Intercept) 0.45851 0.67713 Number of obs: 737, groups: FishID, 107; fSampling, 12Fixed effects: Estimate Std. Error z value Pr(>|z|) (Intercept) 2.1571 3.4374 0.628 0.5303 SL -0.5340 0.2004 -2.665 0.0077 ** aliveFALSE -16.7792 3.6527 -4.594 4.35e-06 ***SL:aliveFALSE 1.0186 0.2124 4.796 1.62e-06 ***
![Page 23: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/23.jpg)
Gupp
ies (HP
) Co
ntrol
Natural resource levels
Gupp
ies (HP
) Co
ntrol
High resources (canopy thinned)
Gupp
ies (LP)
Control
Gupp
ies (?)
Control
evolu+on
x2
Experimental Evolu+on
![Page 24: Beyond Lifetime Reproductive Success: The posthumous reproductive dynamics of Trinidadian Guppies](https://reader034.vdocuments.mx/reader034/viewer/2022042700/55981ab01a28ab36048b4667/html5/thumbnails/24.jpg)