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BENCHTOP FERMENTOR & BIOREACTOR SYSTEMS
A SELECT NEW RANGE OF VERSATILE, HIGH PERFORMANCE 3 - 20L AUTOCLAVABLE SYSTEMS WITH EASY-TO-USE TOUCHSCREEN CONTROLS
www.cleaverscientifi c.comwww.cleaverscientifi c.com
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Cleaver Scientifi c is proud to launch an exciting new range of high performance fermentors
and bioreactors.
Remarkably user-friendly and equipped with intuitive controls, these fermentors and bioreactors are suitable for most fermentation and cell culture applications performed by:
• University teaching labs;
• Life science research labs;
• R&D facilities within biofuel and biotech companies;
• Pharmaceutical organisations;
• Food and beverage manufacturers.
Each system is supplied as a customised solution with vessel, impeller, control station and probes for growth of diff erent microbes, yeast, mammalian, insect and plant cells, removing the complexities often faced with other brands of equipment selection and set up.
Assembly is straight forward with the unit being unpacked and made ready for the autoclave in less than an hour.
The control station is fully integrated with built-in peristaltic pumps, gas fl ow control, and pH, dissolved oxygen (DO), substrate feed and antifoam functionalities all installed in one bench top unit.
An easy-to-use large 10.4-inch colour touchscreen interface allows setting and real-time pictorial display of agitation speed, temperature, pH and dissolved oxygen (DO), while 4 assignable built-in peristaltic pumps act in tandem with the corresponding probe sensor to control pH, DO and antifoam feeds.
Each probe is connected to an easily accessible side panel that also houses quick-fi t connectors for rapid set up of the heating system and ancillary equipment for aeration and cooling.
...Introducing a new player in bench top fermentors and bioreactors !
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Changing with your needs
Grow almost any cell type − With a wide range of vessels and impellers, including
customised options, Cleaver Scientifi c fermentation and bioreactor systems have the
capacity to grow virtually any cell type: whether it’s anaerobic or aerobic microbes, yeast,
insect, plant or mammalian cells.
Pre-programmed for fermentation & cell culture − All Cleaver Scientifi c fermentors
and reactors are pre-programmed with fermentation and cell culture operating modes
allowing each system to adapt as your needs change.
Diff erent vessel confi gurations − All fi ve types of interchangeable single-wall,
double-wall, heat-blanketed, jacketed and air lifter vessels are available in 3 to 20 litre
volumes and made from autoclavable borosilicate.
Multiple options & accessories − Full adaptability is assured with up to 4 probe
options for pH, DO and temperature; 4 brushless motor drives with 12 choices of impeller;
2 adjustable fl ow meters, and 5 vessel types in as many as 6 diff erent volumes.
Simple to use
Touch-and-grow − A large 10.4” colour touch screen interface built within the control
station allows the user to control and view each parameter within a conspicuous, easy-to-
read pictorial display.
Helpful screen tabs − The colour touch screen is pre-confi gured for use with the
selected reactor vessel, while 8 diff erent screen TABS facilitate user-fi endly control of all
critical process values within a single screen (see page 5).
Same software on all units − The same control software features on all fermentors
and bioreactors allowing the user to change to other Cleaver Scientifi c systems when
required, while ethernet remote control is available without additional software.
upgrades.
Automatic Controls
Gas fl ow control − Automatic multi-gas mixing options - up to two and four
respectively in fermentation and cell culture mode - ensure optmised yields and cell
growth, while a selection of customisable gas fl ow options provide complete fl exibility by
allowing customers to design systems specifi c to their needs.
Adjustable air fl ow − Each system features as standard an adjustable air fl ow
rotameter on the front of the control station. An optional oxygen (O2) enrichment module
comprising adjustable rotameter and O2 enrichment valve may also be fi tted. Bespoke
thermal mass fl ow control (TMFC) options are available on request.
2-stage cascade with P-I-D technology − A built-in 2-stage ‘cascade’ feature utilises
P-I-D (Proportional-Integral-Derivative) control technology to maintain the DO setpoint
through automatic adjustment of agitation speed and substrate feeds, and optional O2
enrichment. The adjustable P-I-D values for pH and DO may be defi ned automatically by
the size of the reactor vessel, or after manual fi ne-tuning by the user.
Leave it to its own devices − P-I-D control of each setpoint obviates the need for
constant operator supervision, thus aff ording the user the time to perform other tasks at
their convenience while the system remains unattended. Choose glass-walled vessels as shown or heat-blanketed vessels. Customise your system with up to two rotameters or optional TMFC for gas overlay and sparge.
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A wide range of accessories, including multiple probe options, ensure easy customisation of each bench top fermentation and cell culture system for many diff erent cell types and microbes. From left to right, probes include: (1.) pH, (2.) DO, (3.) temperature, (4.) antifoam and (5.) oxidoreduction potential (ORP).
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A low maintenance, brushless 24V DC motor (1.) is avail-able as standard with each system and may be used with both types of standard impeller, such as the Rushton-style, as shown (2.), for fermentation, or the low-shear pitched blade impeller (3.) for more fragile insect, plant and ani-mal cultures. Bespoke impellers are available on request for full fl exibility.
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Heat-blanketed dish bottom vessels − available in 6 sizes, ranging from 3 to 20L. Larger 15L and 20L vessels are ideal for process scale-up as cost-eff ective
alternatives to expensive sterilisable-in-place systems (SIP). Dry-heating blanket suitable for growth of microbes and non-photosynthetic plant cells.
Single-wall plain bottom vessel with heating base − available in 4 sizes: 3, 5, 7.5 and 10L. Dry-heating base ensures rapid heating and cooling. Perfect entry-level system for microbial fermentation.
Low maintenance 30-1200rpm brushless motor − available for all models. Supplied with 'quick' connector for easy vessel connection. Optional low 5-300rpm motor recommended for cell culture.
Side panel − provides convenient access to all connections for probe sensors and motor, as well as the sparger, cooling system and condenser.
10.4-inch colour touchscreen interface − included as standard with all units. Helpful screen tabs guide you through each step of the set-up process. 'Get touching and start growing!'
Double-wall round bottom vessels − available in 4 sizes: 3, 5, 7.5 and 10L. Warm or cool water circulates through the outer wall cavity for thermostatic temperature control. Especially suitable for growth of mammalian, microbial and insect cells.
Four peristaltic pumps − may be linked directly to acid, base, antifoam and substrate feeds. Pump speed adjustable within 1rpm increments.
Up to two rotameters − one for gas fl ow control with optional second rotameter for O2 enrichment.Stop-Start −convenient front access.Small footprint − 40x50cm (WxD) - allows control station to fi t most benches comfortably.
Air-lifter vessels − available in double-jacketed (1) and single-wall (2) formats - facilitate unique impeller-free growth of fragile plant and fungal cells using micro-sparger and adjustable draft tube.
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Sensor panel − with connection ports for pH, ORP, DO, antifoam and vessel temperature probes; gas mixing station and motor; and dry-heating base unit / blanket and blanket temperature probe (on CSL FS-02 models only).
Air and cooling panel − with connection ports for condenser cooling and vessel cooling-coil water inlets and outlets; condenser fl ow regulator; main water inlet and outlet drain; gas outlet to sparger; and air and O2 inlets.
USB port − for data export to USB fl ash drive or computer.
Remote control and system expansion panel − includes extension ports, RE-485 connection for ancillary fi fth peristaltic pump, power socket and ethernet port for connection to optional laptop shown.
'Get touching and start growing!'
Control Station and Vessels
Side Panel
Simple to use touchscreen interface facilitates set up in less than an hour. No confi guration needed.
ilable in double
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Threaded penetrations in 316L stainless steel headplate − provided for probes; acid base and substrate feeding ports; septum port for small volume injection (e.g. antibiotics); condenser to prevent culture media evaporation; sparger, cooling ports, and inoculation and sampling ports. T-shaped hand-lifters − for convenient vessel transfer and manoeuvrability.
Sample Control Screens
1. MONITOR - shows a full summary of all critical processes within one easy-to-read screen, including: operation status; setpoint and present value of each parameter; user login and working status of antifoam, pH and substrate feeds; process time and manual or automatic operation.
2. PUMP - for simultaneous control, calibration and assignment of all 4 peristaltic pumps, plus fi fth ancillary pump if used.
3. CONTROL - performs manual or automatic control of pH, agitation, temperature and DO setpoints.
4. SETTING - allows selection of the program schedule for each automatic process.
5. CHART - shows real time recording of trend data for each fermentation or cell culture process
6. CALIBRATION - for accurate calibration of each probe sensor.
7. MAINTENANCE - to set up a service and maintenance schedule.
6. CALIBRATIONTIO - for accurate calibration oftion o
7. MAINTENANCE -ANCE -7. MAINTENA to set up a service anda service andto set up a
External peristaltic pumps − may be connected to control station by RE-485 port. Fifth external pump may be controlled in the PUMP screen tab (see sample screen 2 below). Pump head is reversible and may be used with any one of six silicon tubes, ranging from 0.8 to 7.9mm in internal diameter, depending on the desired fl ow rate (36 to 2300 mL/min). Silicon tubes supplied in 7.6m lengths.
Vessel Headplate
Ancillary Pump
Each screen may be viewed and programmed using ethernet remote control with optional lap top.
8. HELP - for set up and operation assistance, particularly the cooling and air system following sterilisation.
Single-wall round bottom vessels − available in 4 sizes: 3, 5, 7.5 and 10L. Inner circulation loop facilitates rapid heating and cooling. Ideal for anaerobic and aerobobic fermentation.
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TECHNICAL SPECIFICATION*
Th ermostatic Bioreactor and Fermentation Systems (CSLFS-01)Double-wall round bottom vessel systems Single-wall round bottom vessel systems Air-lifter vessel systems
Double-wall Single-wall
VESSEL
Series CSLFS-V-A CSLFS-V-B CSLFS-V-C
Working volume 3L 5L 7L 10L 3L 5L 7L 10L 5L
Total volume 3.7L 6.8L 8.9L 12.5L 3.7L 6.8L 8.9L 12.5L 6.9L
Design All vessels comprise autoclavable borosilicate glass contruction with 316L stainless steel headplate with fi ttings
CONTROL
STATION
Design features(A) Compact control station with integrated controller including built-in pumps and side panel capable of supporting utilities; (B) storage for 59,994 process programmes; (C) log data storage for 10 process monitoring fi les; (D) real-time trend data recording; (E) industrial standard ABS front panel with painted iron housing
Display 10.4-inch (265mm) colour touchscreen interface supplied as standard with each control station
Function Fermentation and cell culture monitoring and control
Dimensions Footprint (W x D): 400 x 500mm (15.75 x 19.69"); Height: 740mm (29.13")
TEMPERATURE
Range 5˚C above coolant to maximum 60˚C
Control
(A) Thermostatic system with programmable 15-step PID control; (B) built-in heat exchanger (400W heater / water circulation pump); (C) automatic cooling water valve
Double-wall water jacket heater and circulation loop (see B and C above)
Single-wall vessel with inner coil heater and circulation loop (see B and C above)
Double-wall water jacket heater and circulation loop (see B and C above); no temperature control for single-wall vessels
Probe Platinum RTD probe (Pt100)
AGITATION
Drive Removable top 'brushless' motor n/a
Range 30-1200rpm standard motor for routine fermentation and cell culture; 5-300rpm option for shear sensitive cell lines n/a
Control Manual setting or programmable 15-step PID control n/a
Impellers2 pieces of adjustable pitched-blade impeller for fragile cell types
3 pieces of adjustable Rushton-style impeller for less shear-sensitive microbial cell types
n/a
Baffl es Removable 316L stainless steel baffl e for fermentation n/a
AERATION
Gas fl ow optionsStandard built-in air-fl ow rotameter (1-10Lpm for 3/5L or 2-20Lpm for 7/10L vessels); optional O2 enrichment module with rotameter and enrichment valve (bespoke TMFC possible - please enquire)
Standard built-in air-fl ow rotameter (1-10Lpm for 5L vessels); adjustable draft tube; O2 enrichment optional
Gas mixing Manual / automatic air and O2 mixing; additional 4-gas mixing station available
Sparger Orifi ce ring sparger supplied as standard Micro-sparger
Inlet fi lter 0.2μM absolute fi lter
pH
Range 2-14 pH; 0.01pH resolution
Control PID, linked to acid/base pumps; one-point control with adjustable deadband (pH control range)
Sensor Gel-fi lled, autoclavable pH probe
DO
Range 0-200%; 0.1% resolution
ControlPID, 2-stage cascade: starting with agitation (1), cascading to optional O2 enrichment (2) if agitation speed is maximum but DO is below setpoint; substrate feeding programme (3) activated at DO setpoint
Sensor Autoclavable, polarographic DO probe
ANTIFOAMControl On/off
Sensor 316L stainless steel with insulated Tefl on tube
EXHAUSTFilter 0.2μM absolute fi lter
Condenser 316L stainless steel
PUMPS Control 4 built-in, assignable, with manual setting or programmable 15-step PID control; 0-65rpm (RE-485 connection on control station for fi fth pump option)
UTILITIES Water / gas / power 2 bar maximum (29 PSI) / 1 bar maximum (14.5 PSI); dry and oil-free / 100-120V 50/60Hz or 210-230V 50/60Hz; electrical safety cut-off switch
*Specifi cations may be subject to change without notice
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TECHNICAL SPECIFICATION*
Dry-Heating Bioreactor and Fermentation Systems (CSLFS-02)Heat-blanketed single-wall round bottom vessel systems Single-wall plain bottom vessel systems with heating base
VESSEL
Series CSLFS-V-B CSLFS-V-D
Working volume 3L 5L 7L 10L 15L 20L 3L 5L 7L 10L
Total volume 3.7L 6.8L 8.9L 12.5L 18L 24.4L 3.7L 6.8L 9.1L 13L
Design All vessels comprise autoclavable borosilicate glass contruction with 316L stainless steel headplate with fi ttings
CONTROL
STATION
Design features(A) Compact control station with integrated controller including built-in pumps and side panel capable of supporting utilities; (B) storage for 59,994 process programmes; (C) log data storage for 10 process monitoring fi les; (D) real-time trend data recording; (E) industrial standard ABS front panel with painted iron housing
Display 10.4-inch (265mm) colour touchscreen interface supplied as standard with each control station
Function Fermentation and cell culture monitoring and control
Dimensions Footprint (W x D): 400 x 500mm (15.75 x 19.69"); Height: 740mm (29.13")
TEMPERATURE
Range 5˚C above coolant to maximum 90˚C
Control(A) Dry-heating system with programmable 15-step PID control; (B) automatic solenoid-controlled cooling water valve
Dry-heating blanket and cooling loop (see A and B above) Dry-heating base unit and cooling loop (see A and B above)
Probe Platinum RTD probe (Pt100)
AGITATION
Drive Removable top 'brushless' motor
Range 30-1200rpm standard motor for routine fermentation and cell culture; 5-300rpm option for shear sensitive cell lines
Control Manual setting or programmable 15-step PID control
Impellers 3 pieces of adjustable Rushton-type impeller for less shear-sensitive microbial cell types (2 pieces of adjustable pitched-blade impeller for fragile cell types - optional)
Baffl es Removable 316L stainless steel baffl e for fermentation
AERATION
Gas fl ow optionsStandard built-in air-fl ow rotameter (1-10Lpm for 3/5L; 2-20Lpm for 7/10L; 4-50Lpm for 15/20L vessels); optional O2 enrichment module with rotameter and enrichment valve (bespoke TMFC possible - please enquire)
Gas mixing Manual / automatic air and O2 mixing; additional 4-gas mixing station available
Sparger Orifi ce ring sparger supplied as standard
Inlet fi lter 0.2μM absolute fi lter
pH
Range 2-14 pH; 0.01pH resolution
Control PID, linked to acid/base pumps; one-point control with adjustable deadband (pH control range)
Sensor Gel-fi lled, autoclavable pH probe
DO
Range 0-200%; 0.1% resolution
ControlPID, 2-stage cascade: starting with agitation (1), cascading to optional O2 enrichment (2) if agitation speed is maximum but DO is below setpoint; substrate feeding programme (3) activated at DO setpoint
Sensor Autoclavable, polarographic DO probe
ANTIFOAMControl On/off
Sensor 316L stainless steel with insulated Tefl on tube
EXHAUSTFilter 0.2μM absolute fi lter
Condenser 316L stainless steel
PUMPS Control 4 built-in, assignable, with manual setting or programmable 15-step PID control; 0-65rpm (RE-485 connection on control station for fi fth pump option)
UTILITIES Water / gas / power 2 bar maximum (29 PSI) / 1 bar maximum (14.5 PSI); dry and oil-free / 100-120V 50/60Hz or 210-230V 50/60Hz; electrical safety cut-off switch
*Specifi cations may be subject to change without notice
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DOUBLE-WALL ROUND BOTTOM SYSTEMSEach system includes: -
Controller Thermostatic system control station with built-in rotameter & 4x built-in peristaltic pumps
Vessel Double-wall vessel (including glass body, head plate, T-shaped hand-lifters & 2 probe adaptors); 2 pieces adjustable pitched-blade impeller; & baffl e, condenser & sparger assemblies
Motor Agitation motor Probes 1x pH probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable
Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc fi lters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench
PART NUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION
CSLFS-01-A03P-110/220VComplete thermostatic bioreactor and fermentation system with
double-wall round bottom vessel, 3LCSLFS-01-A07P-110/220V
Complete thermostatic bioreactor and fermentation system with
double-wall round bottom vessel, 7L Mammalian & insect cell culture
CSLFS-01-A05P-110/220VComplete thermostatic bioreactor and fermentation system with
double-wall round bottom vessel, 5LCSLFS-01-A10P-110/220V
Complete thermostatic bioreactor and fermentation system with
double-wall round bottom vessel, 10L
SINGLE-WALL ROUND BOTTOM VESSEL SYSTEMSEach system includes: -
Controller Thermostatic system control station with built-in rotameter & 4x built-in peristaltic pumps
Vessel Single-wall vessel (including glass body, head plate, T-shaped hand-lifters & 2 probe adaptors); 3 pieces adjustable Rushton-style impeller; & baffl e, condenser & sparger assemblies
Motor Agitation motor Probes 1x pH probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable
Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc fi lters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench
PART NUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION
CSLFS-01-B03P-110/220VComplete thermostatic bioreactor and fermentation system with
single-wall round bottom vessel, 3LCSLFS-01-A07P-110/220V
Complete thermostatic bioreactor and fermentation system
withsingle-wall round bottom vessel, 7L Anaerobic & aerobic microbial
fermentationCSLFS-01-B05P-110/220VComplete thermostatic bioreactor and fermentation system with
single-wall round bottom vessel, 5LCSLFS-01-A10P-110/220V
Complete thermostatic bioreactor and fermentation system with
single-wall round bottom vessel, 10L
AIR-LIFTER VESSEL SYSTEMSEach system includes: -
Controller Thermostatic system control station with built-in rotameter & 4x built-in peristaltic pumps
Vessel Air-lifter vessel (including glass body, head plate, draft tube, T-shaped hand-lifters & 2 probe adaptors), condenser & micro sparger assemblies
Motor n/a Probes 1x pH probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable
Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc fi lters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench
PART NUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION
CSLFS-01-C051P-110/220VComplete thermostatic bioreactor and fermentation system with
single-wall air-lifter vessel, 5LCSLFS-01-C052P-110/220V
Complete thermostatic bioreactor and fermentation system with
double-wall air-lifter vessel, 5L
Culture of plant & shear-sensitive cells
HEAT-BLANKETED SINGLE-WALL ROUND BOTTOM VESSEL SYSTEMSEach system includes: -
Controller Dry-heating system control station with built-in rotameter & 4x built-in peristaltic pumps
Vessel Single-wall round bottom vessel with heating blanket (including glass body, head plate, draft tube, T-shaped hand-lifters & 2 probe adaptors); 3 pieces adjustable Rushton-style impeller; & baffl e, condenser & sparger assemblies
Motor Agitation motor Probes 1x pH probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable
Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc fi lters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench
PART NUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION
CSLFS-03-B03P-110/220VComplete dry-heating bioreactor and fermentation system with
single-wall round bottom vessel, 3LCSLFS-02-B10P-110/220V
Complete dry-heating bioreactor and fermentation system with
single-wall round bottom vessel, 10L Fermentation of anaerobic & aerobic microbes & culture
of temperature- and shear-sensitive cells
CSLFS-02-B05P-110/220VComplete dry-heating bioreactor and fermentation system with
single-wall round bottom vessel, 5LCSLFS-02-B15P-110/220V
Complete dry-heating bioreactor and fermentation system with
single-wall round bottom vessel, 15L
CSLFS-02-B07P-110/220VComplete dry-heating bioreactor and fermentation system with
single-wall round bottom vessel, 7LCSLFS-02-B20P-110/220V
Complete dry-heating bioreactor and fermentation system with
single-wall round bottom vessel, 20L
SINGLE-WALL PLAIN BOTTOM VESSEL SYSTEMS WITH HEATING BASEEach system includes: -
Controller Dry-heating system control station with built-in rotameter & 4x built-in peristaltic pumps
Vessel Single-wall plain bottom vessel (including glass body, head plate, T-shaped hand-lifters & 2 probe adaptors) with heating base; 3 pieces adjustable Rushton-style impeller; & baffl e, condenser & sparger assemblies
Motor Agitation motor Probes 1x pH probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable
Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc fi lters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench
PART NUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION
CSLFS-01-D03P-110/220VComplete dry-heating bioreactor and fermentation system with
single-wall plain bottom vessel, 3LCSLFS-01-D07P-110/220V
Complete dry-heating bioreactor and fermentation system with
single-wall plain bottom vessel, 7LAnaerobic, aerobic &
micro-aerobic microbial
fermentationCSLFS-01-D05P-110/220VComplete dry-heating bioreactor and fermentation system with
single-wall plain bottom vessel, 5LCSLFS-01-D10P-110/220V
Complete dry-heating bioreactor and fermentation system with
single-wall plain bottom vessel, 10L
PART NUMBER
PART NUMBER
PART NUMBER
PART NUMBER
For technical advice & further information & pricing on optional ORP probes, impeller choices, 5-300rpm agitation motor, external peristaltic pump, O2 enrichment
module & TMFC, please contact your distributor or email: sales@cleaverscientifi c.com.
ORDERING INFORMATION