8/14/2019 BEL311 Major

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DEPARTMENT OF BIOCHEMICAL ENGINEERING AND BIOTECHNOLOGYMaior .~xalllination

BEL311: PhysicalandChemicalI'n)pcrtiesof Biom?lecules

Max.Marks: 45 Time: 2 hom',

/Jr(,l' itJ'is the soul (~lcommuJ1icatiol1. E.xtralleOllS il?fiJrnwtion will he pellalized!

I. Show that the wavelength of the emitted photons cannot be longer than the wavelength of t'te cxeitaliull

photons Il)1a Iluorophore.

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Given a microbial cell. provide a protocol (in form of a flow diagram/table) to purify intraccllul:,:proteins. What would be tbe major difference in the methodology for purifying membrane proteiliClearly mark the sleps in the t1ow diagramltable for answering the latter.

2.

(4.j:~ :3. What is thc Iluid-mosaic modd It)[biologicalmembrancs'? What are memhrtme rails? Whal hiocltcll1i..

and hi(Iphysical experimental evidence den}onstratcsthe presenceor membrane raIts.(2 +:2 -1.1 )j

4. Biomolccular asymmetry is an inherent feature of biological membranes. \-vhen they ::n~a part of a livil1!'system. List the asymmetries. Why are these asymmetries abs,.;;nt in syntheti!:~ systems (;'.g. liposol1ll:s\ '

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5. NamIl~/v, Answer the following qucstiol1srcg:mlinL~ thc prolt';I:(us\.' the hydr:')rrl~)biclty cale pro\tided below):,S if (a) How many amino acid residues ,:'ould be expected.in the ~ltllprotein?

~ (:) t (b) I\()\v n~any transmembrane dOI~lallls.docs the protell1 ~i~ve?I . r.cr~ (c) :X-s~umlJ1ghat the values prov.lded III the hydror:hob!clty sca\c bd{)\\ ' .represent ~l1alll "( () G~' In free energy, what can be saId ahout the expertmeptallsyslem used lo!' gcneratlllt: 111' C) 61, scale?(: () 6~ 61 Y r L L-I+- -;

itY scale given by Eisenberg et aI., j, Mol. BioI. (1984):

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