Before and after your SAXS experiment

Dmitri Svergun

© 2016BioStruct-X

EU FP7 access project 2011-2016

• Partner Categories: experimental facilities (red), only R&D (yellow), TID (green)

D.Svergun, coordinator in 2013-2016

© 2016BioStruct-X

EU FP7 access project 2011-2016Through BioStruct-X transnational access activities 1156 scientific projects and 1991 users received support that already resulted in over 340 publications.

BioStructX at EMBL Hamburg - Overview of Access (hours)RP1 RP2 RP3 Total

DORISx11 24 24 MXx12 104 104 MXx13 72 72 MXx33 358 358 SAXSPETRAP12 180 1373 896 2449 SAXSP13 70 387 764 1221 MXP14 18 574 705 1297 MXSPC 0 29.5 82.3 111.8

iNEXT – Infrastructure for Structural Biologyinfrastructure for NMR, EM & X-rays for Translational research

23 partners; started September 1, 2015; synchrotron access as of March, 1, 2016

www.inext-eu.org

iNEXT expands the availability of structural biology services to new communities of users, and inparticular to scientists with backgrounds other than structural biology, including from SMEs

❖ Six NMR centres❖ Berlin, Brno, Florence, Frankfurt, Lyon, Utrecht (coordinator)

❖ Six synchrotron sites ❖ Diamond, EMBL-HH, EMBL-GR, ESRF, Lund, Soleil

❖ Five EM facilities❖ Brno, Diamond, EMBL-HD, Leiden, Madrid

❖ Protein interactions in vivo and in vitro❖ Amsterdam (deputy coordinator), EMBL-HD

❖ Research Partners❖ Aarhus, Lund

❖ Training centres❖ Brno, Budapest, Lisboa, Oulou, Patras, Rehovot

❖ ESFRI Projects❖ ESS, Instruct (and EuroBioImaging, EU-Openscreen affiliated)

The project must be interdisciplinary and have a translational component

Filing in iNEXT applicationhttp://www.inext-eu.org/access/, “submit application”

Three levels of access in iNEXT

• Structural Audit (sample quality assessmentfor non-experienced users)

- Advanced biophysical characterisation, evaluation of protein quantity, quality and stability followed by crystallization trials and SAXS- Mail-in SAXS• Enhanced Support (fully supported access for

non-experienced users)- X-ray structure- NMR data- Cryo-EM sample optimization- Macromolecular interactions- Advanced light imaging- Ligand and fragment screening

• High-End Data Collection (fullcontrol over advanced equipmentfor expert users)

- Synchrotron data (MX and SAXS)- High-field NMR- High-resolution EM

NOTE: Mail-in and Remote access is highly welcome at P12 -- not only within iNEXT!

Accepted iNEXT users can import their iNEXT proposal into the SMIS system

Applying for beamtime at EMBL-Hamburghttps://smis.embl-hamburg.de/, register and submit applicationMain call is at the end of the year for the subsequent year.During the year, rolling proposals are accepted (2-3 months evaluation time)

“Table 1” and other publicationrules for SAS

Recommend-dations of theSAS Task Force of the IUCrcommission

Database development and submission curation is done in Hamburg. Presently offers 263 data sets and 399 macromolecular models