Antibodies

“Our recombinant Fab

Antibodies can improve

assay sensitivity”

Be confident in your results when you

choose BBI’s monoclonal antibodies for

your next IVD assay:

Tested in ELISA

Tested in lateral flow

Recommended pairings provided

Strong customer feedback

NEW: Highly sensitive Recombinant

Fab antibodies to improve your next

generation assays

Custom development and

manufacturing services to meet your

individual requirements

Primary antibodies for diagnostic assays

Top mouse monoclonal antibodies

Lane 1: Native Human D-

Dimer

Lane 2: Human Serum

Lane 3: Native Fibrinogen

Lane 4: Native Plasminogen

Exclusive D-Dimer clones 1D2 and 3B6

Validated in Lateral Flow, Sandwich ELISA,

Western Blot and Latex Agglutination

Does not detect the closely related molecules

Fibrinogen and Plasminogen

Cited in 100’s of peer-reviewed journal

papers

Antibodies for cancer diagnosis

CA15-3 & CA19-9 antibody pairs

Matching pairs validated in sandwich ELISA

against real patient samples

Results correlate with commercially available tests

Top mouse monoclonal antibodies

HbA1c antibodies for Diabetes

Validated in Lateral Flow and Sandwich ELISA

Tested in patient samples

Results correlate with commercial assays

Infectious Disease antibodies

Tested in Lateral Flow

Tested using patient samples

Comprehensive range of biomarkers covered

NEW! Recombinant Fab antibodies

Exceptional

Sensitivity

The range of detection is determined

for all of our recommended pairs

Reliable

Production

Produced in E.coli to shorten lead

times and avoid scaling issues

Phage

Technology Phage technology screens 4500 pairs to

make sure the best pair is discovered

High

Affinities

Picomolar affinities ensure enhanced

antibody binding

Using Phage Display Technology

1m antibody clones generated, enhancing the

opportunity of finding the best antibodies

4500 antibody pairs are screened to ensure the

best pair is identified

Patient sample testing to identify the best pair

Immunisation

of mice

Transfer antibody

coding DNA to

phage

Antibodies selected

for antigen binding

Related antigens

added in excess to

screen out cross

reactions

Candidate genes

transferred to

plasmid and E.coli

Antibodies purified

Matched pair

screening - Luminex

Patient

sample

testing

The development process

Example: Calcitonin Recombinant Fab

antibody

Patients with calcitonin levels >100 pg/mL

have a high risk for medullary thyroid

carcinoma (~90%–100%) Toledo et al.,

(2009)

Our Calcitonin antibody pair detects at a

range of 6 to 2000 pg/mL

These highly sensitive

antibodies are ideal for

evaluating risk of this carcinoma

“Reduce your time and

money on reagents with

unproven performance – our

results speak for

themselves”

Demonstrated Clinical relevance

Recombinant Fab antibodies vs.

standard monoclonal antibodies

Standard Monoclonal Antibodies

Recombinant Fab Monoclonal

Antibodies

Initial Screen 100’s of clones screened 1,000,000,000 clones screened

Matched Pairing Best few antibodies tested for pairing >4500 antibody pairs tested (Luminex

platform)

Affinity micromolar as standard picomolar as standard

Sensitivity microgram/ml as standard picogram/ml as standard

Validation Purified Antigen Clinical samples

Conjugation May interfere with antigen binding

region

Engineered Cysteine residue ensures no

interference

Production

Cell culture - slow, expensive,

unreliable Ascites - slow, uses many

animals, illegal in EU

E. coli - fast, reliable, easy to scale-up,

simple storage

Antigen binding sites per

mg of purchased product Standard 36% more

Demonstrated exceptional performance

Our CRP Antibody has a significantly lower limit of detection than other high-

performance CRP antibodies on the market

Benefit: make assays which are more sensitive than those of your

competitors – increasing effective diagnosis and time to result on a like for

like basis

0

200

400

600

800

1000

1200

10

0

75

50

25

10 5 2 1 0

Peak h

eig

ht

CRP conc (ng/mL)

Comparison of BR228-D4A3 with commercially available antibodies

BR228-D4A3

Competitor A

Competitor B

Flu Fab

Reader

CRP conc (ng/ml) 100 75 50 25 10 5 2 1 0

BBI antibody, BR228-D4A3

Competitor B antibody

Competitor A antibody

Visual

Lateral Flow: Comparison of BR228-

D4A3 with commercially available

antibodies

Our Capabilities | Monoclonal Antibodies

Cardiac Infectious Disease Malaria HRP2 Veterinary

D-Dimer C. difficile Toxin A Malaria LHD (pan) Canine Distemper

Troponin I C. difficile Toxin B Malaria LDH (Pf & Pv) Virus

Chikungunya Norovirus Avian Influenza H5

Diabetes Dengue NS1 PBP2a FeLV

Hb Ebola Reston TB Brucella LPS

HbA1c Ebola Sudan Enrofloxacin

Ebola Zaire Tumour Goat IgG

Drugs of abuse H. pylori CA125 Canine IgM

THCΔ9 HAV CA15-3 Chicken IgY

Hepatitis B CA19-9 GST

Hormones HIV p24 Calcitonin

Progesterone HIV gp35

HIV gp41 Immunoglobulins

Pregnancy Influenza A IgA

AFP Influenza B IgE

Leptospira

Listeria

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Our Capabilities | Polyclonal Antibodies

Inflammation Pregnancy Renal

CRP AFP A2M

B2M

Immunoglobulins Inflammatory Cystatin C

IgA A1AT

IgM

IgG Haematology Metabolism

Albumin Haptoglobin Ceruloplasmin

Ferritin

Haemoglobin

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Our Capabilities | Recombinant Fab Antibodies

* Detection Ranges are assay dependent and optimised for specific

clinical conditions, therefore may be improved with optimisation.

Biomarker Detection Range*

Capture

Antibody Detection Antibody

Adiponectin 0.1 - 525 ng/mL BR101-P7E2 BR101-M3H8

Adiponectin 230 - 59118 ng/mL

BR101-H2B4 (Competitive ELISA)

Angiopoietin 2 0.001 - 97 ng/mL BR103-P4E8 BR103-V5F2

Big Endothelin 1 0.001 - 1.8 ng/mL BR104-D6A4 BR104-H1A4

Calcitonin 0.006 - 2 ng/mL BR388-G6B1 BR388-D5C4

Cathepsin D 0.03 - 150 ng/mL BR107-F5G2 BR107-K6D3

CKBB 0.15 - 33 ng/mL BR112-V6F6 BR112-Z1C4

CRP 151 - 231000 ng/mL

BR228-D4A3 (Competitive ELISA)

Cystatin C 165 - 9073 ng/mL

BR175-G8D9 (Competitive ELISA)

Cystatin C 165 - 9073 ng/mL

BR175-H4C8 (Competitive ELISA)

E. histolytica not tested BR116-K9F7 BR116-G3A1

Eotaxin 0.007 - 2 ng/mL BR108-M7H3 BR108-K2D9

E-selectin 0.05 - 235 ng/mL BR117-K2E7 BR117-H5B6

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Our Capabilities | Recombinant Fab Antibodies

* Detection Ranges are assay dependent and optimised for specific

clinical conditions, therefore may be improved with optimisation.

Biomarker Detection Range* Capture Antibody

Detection

Antibody

FABP Intestine 0.65 - 100 ng/mL BR115-F7G7 BR115-G6B9

FABP Liver 0.03 - 90 ng/mL BR106-G5A2 BR106-P9F5

G. lamblia not tested BR120-P3F2 &

BR120-T1G8

HSP60 0.1 - 1200 ng/mL BR100-G2A7 BR100-H4C2

Human Fetal Hemoglobin 1.9 - 305 ng/mL BR121-T5H9 BR121-V5A7

Leptin 3.9 - 691 ng/mL BR123-M4D6 BR123-P4G2

MMP7 0.1 - 293 ng/mL BR124-S4E3 BR124-T6G8

MPO 2 - 1200 ng/mL BR240-N5G6 BR240-D8B4

Neutrophil Elastase 0.008 - 33 ng/mL BR127-H2A4 BR127-G7C2

Proinsulin C-Peptide 0.09 - 38 ng/mL BR143-G5D3 BR143-K7C9

S100 Beta 0.04 - 5.6 ng/mL BR332-G4B9 BR332-F5H7

ST2 1.39 - 97 ng/mL BR139-S7B7 BR139-V8D4

ST2 0.08 - 31 ng/mL BR139-T3G5 BR139-V8D4

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