Basics of Chromatography
• Ability to dissolve in mobile phase
• Interaction with stationary phase
• Time on X axis• Response on Y
• Separation dependent on time on the column (along with other factors)
• Retention time of the analyte• Retention time of the Mobile Phase
Basics of Chromatography
• Average linear flow rate
• Average linear flow of mobile phase
Rt
L
Mt
L
• Plate Height • Theoretical Measure
of Efficiency
LH
2
• Fig. 26-6• Determining standard deviation from a peak• Std dev = τ W = 4 τ
Basics of Chromatography
• The Van Deemter Equation
H = A + B/u + Cu
• Multiple Flow Paths• Tortuosity
• Components of the VanDeemter
• How Plate Height is effected by particle size in solid packing
The Effect of Mobile Phase on Retention