Isolating DNA from a banana…yes a banana!!!

How do we take out the DNA?

1. a) Salt

Helps DNA keep together during the process.

How do we take out the DNA?

1. b) Detergent (shampoo)

Breaks down the cell membrane

and nuclear membrane.

(NO BUBBLES!!)

Filter

2. Filter the banana “goo” with a coffee filter.

-This removes everything that you do not need.

-Take the filtrate.

Banana goo

What you need!!!

"Clean" the DNA

Use a meat tenderizer to digest any proteins associated with the DNA.

Now we remove the DNA

- Add iced rubbing alcohol.

- This will allow the DNA to

precipitate.

- Now you may remove the

DNA with a toothpick.

Instructions and Measurements

Materials: (per group)

• piece fruit (~1/4 of a banana)• 1 coffee filter• two cups (or beakers ~100 mL)• 15 ml of iced alcohol• ~1.5 g (1-2 mL) table salt (NaCl)• 10 mL water• 30 mL liquid soap• 1 toothpick