BAKTERIOLOGI INFEKSI KULIT & JARINGAN LUNAK

DICKY BAGUS WIDHYATMOKO

18 – 19 MARET 2015DEPARTEMEN MIKROBIOLOGI KLINIK, FAKULTAS KEDOKTERANUNIVERSITAS AIRLANGGA

POKOK BAHASAN

Staphylococcus spp

Mycobacterium leprae

DISEDIAKAN

Staphylococcus :

S. aureus

S. epidermidis

S. saphrophyticus

Mycobacterium leprae :

slide only

Uji Katalase

Uji Koagulase

Mannitol Salt Agar (MSA)

Novobiocin test

MRSA Identification

Identifikasi Staphylococcus

Uji Katalase

Uji Koagulase

Mannitol Salt Agar (MSA)

Novobiocin test

Staphylococcus spp

Use of Catalase Test

The catalase test is primarily used todistinguish among Gram-positive cocci

Staphylococcus are catalase-positive,

members of the genera Streptococcus andEnterococcus are catalase-negative.

Catalase Test

Metode :

slide (drop) method

tube method

tube (slant) method

Catalase2H2O2 O2 + 2H2O

Streptococci vs. Staphylococci

Catalase Test

Catalase POS

Staphylococcus

Catalase NEG

Catalase Test

Catalase Test (tube method)

Catalase Test (tube (slant) method)

Coagulase Test

differentiates strains of Staphylococcus aureusfrom S. epidermidis and other coagulase-negative species

S. aureus strains are usually capable ofcoagulating EDTA-treated plasma in the tubetest and will produce clumps of cells in theslide test.

Coagulase Test

method :

Slide Test

Tube Test

Coagulase Test (Slide Test)

S. aureus

Coagulase

Fibrinogen Fibrin

Differential Characteristics

Coagulase NEG

Coagulase POS

Staphylococcus aureus

Mannitol Salt Agar

Contains :

Mannitol

Phenol Red as a pH indicator (pH = 7.8)

Reaction

Mannitol Fermented acid produced

pH < 6.9 : yellow color

pH > 8.4 : red color

various spesies on Tripticase Soy Agar (TSA)

A. S. aureus B. S. epidermidis C. E. coli

various spesies on Tripticase Soy Agar (TSA)

A. S. aureus B. S. epidermidis C. E. coli

Novobiocin Test

Differentiate between S. epidermidis w/ S. saprophyticus

Using Novobiocin disc ( 5 g)

< 15 mm = Resisten S. saprophyticus

Identifikasi MRSA

MRSA : Methicillin-Resistant Staphylococcus aureus

Methicillin Oxacillin Cefoxitin

CLSI M100

Using Cefoxitin disc :

21 mm : MRSA

> 21 mm : non - MRSA

Suspect MRSA

Collect Sample from wound

Collect Sample from nose

Mycobacterium Leprae

Sample : Skin scrap using scalpel

Using Slide with Ziehl-Neelsen Stain method

Presence of globi (singular = globus):encapsulated amphophilic masses of non-viable bacilli found within macrophages or inextracellular space

Result : (Bacterial Index)

1+ At least 1 bacillus in every 100 fields.

2+ At least 1 bacillus in every 10 fields.

3+ At least 1 bacillus in every field.

4+ At least 10 bacilli in every field.

5+ At least 100 bacilli in every field.

6+ At least 1000 bacilli in every field.

On Hand

Pengamatan & Pelaporan Preparat Demo

Uji Katalase

Kultur Media dari spesimen langsung (amies swab) dengan :

Blood Agar Plate

McConkey Agar