Baculovirus Molecular BiologyG.F. Rohrmann

Figure 6.3. Capping baculovirus mRNA. The four modifications that occur during capping are summarized in the following steps: 1) LEF-4 has the potential to dephosphorylate the 5' end of mRNA; 2) and to transfer guanosine to the 5' end of the mRNA; 3) the guanosine is methylated--enzyme is unknown; 4) The 2' OH group of the terminal A is methylated by an unknown methyltransferase. The final structure is shown at the bottom.