bact/alert blood culture - biomérieux draw with blood collection set or worksafe blood culture...
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1. Skin Preparation PDI® Chlorascrub™ Swabstick
• Afterlocationofthevein,vigorouslyscrubthevenipuncture sitewithPDI®Chlorascrub™Swabstickfor30seconds.1
• Allowthesitetoairdrybeforevenipuncture.
•Do not re-palpate the vein.
2.BottlePreparation • Inspecteachbloodculturebottlebeforeusetoensureintegrity
ofbottleandsensoronbottomofbottleisintact.Thesensor isnormallyauniformgrayish-greencolorandayellowcolor wouldindicatecontaminationofthebroth.Discardanybottle foundtobedamagedorwithasensorthatisyellow.
• Removeprotectivefliptopovercap.
•NOTE:Theseptumisnotsterileandmustbedisinfected.
• Cleansetheseptumwith70%alcohol.
•Thebottlehasbeenpre-markedwith5mlincrements. Markthedesiredfillvolumelevelonthebottlefor10ml.
3.VenipunctureandBottleInoculation Direct Draw with Blood Collection Set or WorkSafe™ Blood Culture Collection Kit
• Collectallnecessarymaterialsforadirectdraw:
from diagnosis,the seeds of better health
BacT/ALERT® Blood Culture Collection Procedure
AvailabilityWorkSafeBloodCultureCollectionKit 279102-279105*
Saf-THolder®BloodCultureDevice(AdaptorCapandInsert) 100/case 96004**
*seebioMérieuxcatalogue **seeSmithsMedicalcatalogue
bioMérieux, Inc.100RodolpheStreetDurham,NC27712U.S.A.Tel: (800)682-2666Fax: (800)968-9494
bioMérieux Canada, Inc.7815Henri-BourassaBoulevardOuestSt.Laurent(Québec)Canada H4S 1P7Tel:(514)336-7321Fax:(514)807-00151-800-361-7321ext.221 www.biomérieux-usa.com
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Tips ’n’ Hints•Recommendedbloodtobrothratiois1:5
to1:10.Asthevolumeofblooddrawnisincreased,theyieldofpositiveculturesincreases.Optimally,20mlofbloodshouldbedrawnfromadults(10mlperbottle).
•Whenlabelingthebottles,donotcoverthe peel-offsectionofthebarcodelabelsorthe lotnumbers.
•Orientadditionalpatientlabelverticallyfor scanningefficiency.
•Forbestvolumecontrol,markfilllevelonsideofbottlepriortocollection.
•Donotoverfillthebottles,asthismaycause falsepositivereadings.
•WhenusingtheSaf-THolder®BloodCultureDevice(AdaptorCapandInsert),besureluerisconnectedfirmlyandtheneedleisstraightwhenenteringandleavingtheseptum.Twistingorturningthebottlemayincreasethechancethatthesheathmaynotretractandreseal.
•Toavoidcontaminationofthebloodculturesample,inoculatebloodculturebottlesfirst. Thenfilladditionalbloodcollectiontubes.
Septicemiaoccursinapproximately200,000patientseachyear,andbetween30and50%ofthosepatientsdie.Becausethe consequencesofsepticemiaaresoserious,rapidandaccuratediagnosisofthisdisorderiscritical. TheBloodCultureisthebestdiagnostictoolfordetectingsepticemia,andit’sabsolutelyimperativethatthebloodspecimenbecollectedproperlytoprovideclinicallyvaluableresults. BacT/ALERT®3Dinstrumentsareautomatedsystemsthatincubate,shakeandmonitorculturebottlesforsignsofmicrobialgrowth. SamplesfrompatientssuspectedforsepticemiaareinoculatedintoBacT/ALERTculturebottlesformonitoring.Thebottlesarecolor codedforeasyidentificationofmediatypeandaremadeofplasticforaddedsafety.
SPECIMEN BAG
FROZEN REFRIGERATE
ROOM TEMPERATURE
BIOHAZARD
Performvenipuncture.Whentheneedleisinthevein,secureitwithtapeorholditinplace.
2.
SPECIMEN BAG
FROZEN REFRIGERATE
ROOM TEMPERATURE
BIOHAZARD
PlaceAdaptercapontheaerobic(blueorgreenlabel)BacT/ALERTculturebottleseptumandpressdowntopenetrateandobtainbloodflow.HoldtheAdapterCapdownon thebottle.
3.
SPECIMEN BAG
FROZEN REFRIGERATE
ROOM TEMPERATURE
BIOHAZARD
Usingthefillindicatorlinesonthelabel,obtain8-10mlofblood.MovetheAdapterCapfromtheaerobicbottletotheanaerobic(purpleororangelabel)bottle(ifrequired)andcontinuethecollection.
4.
SPECIMEN BAG
FROZEN REFRIGERATE
ROOM TEMPERATURE
BIOHAZARD
Ifadditionalbloodisrequiredforothertests,placetheAdapterInsertintotheAdapterCapandsnapintoplace.Thismakesthecapcompatiblewithvacuumcollectiontubes.
5. SPECIMEN BAG
FROZEN REFRIGERATE
ROOM TEMPERATURE
BIOHAZARD
Whenfinaltubeisfilled,removetourniquet.Terminatethevenipuncture,activatethesafetyshield,grabthewingwithonehandandslidethewingsbackuntilitlocksinplace.
6. SPECIMEN BAG
FROZEN REFRIGERATE
ROOM TEMPERATURE
BIOHAZARD
Disposeofwing andadaptercapin asharpscontainer.
7. SPECIMEN BAG
FROZEN REFRIGERATE
ROOM TEMPERATURE
BIOHAZARD
Labelspecimensandplaceinplasticbiohazardziplockbag.Bottlescannowbesafelytransportedviapneumatictubesystem.
8.
OR
GatherTourniquet,BacT/ALERT®bottles, Saf-THolder®Adapter,wing,Chlorascrub™Swabstick,gauze,alcoholpreppads.
1perbioMérieux/Cumitechguidelinesasthemanufacturercannotmakethispreptimerecommendation
1a.OpentheWorkSafeBloodCultureCollectionKitwhichcontainsallthenecessarymaterialsinasingle,convenientpackage.
1b.