axion dsa system: a reference notebook of dsa applications--first edition, 2012
DESCRIPTION
Mass Spec Results in Second - No Chromatography or Method Development Required The innovative AxION DSA technology was developed for the rapid direct analysis of solid, liquid or gaseous samples. It offers a novel alternative to standard chromatographically interfaced solutions. Laboratories can now screen, compare and confirm virtually any sample, by introducing it directly to a mass spectrometer in a matter of seconds. This reference notebook of direct sample analyses, highlights the performance characteristics of the AxION DSA. It features a diverse selection of applications, sample types and results across a broad array of industries.TRANSCRIPT
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AxION DSA System A Reference Notebook of DSA Applications—First Edition, 2012
MASS SPEC RESULTSIN SECONDS WITHDIRECT SAMPLE ANALYSIS
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2www.perkinelmer.com/DSA
REVOLUTIONIZE YOUR MASS SPECWORKFLOW Going from sample to results can often be complex
and time-consuming. That is why PerkinElmer has introduced a unique solution: the AxION® DSA (Direct
Sample Analysis) system. Nothing simplifies workflows more than AxION DSA. Diminish cumbersome method development, eliminate chromatography and dramatically reduce sample preparation.
Results in secondsThe innovative AxION DSA technology was developed for the rapid direct analysis of solid, liquid or gaseous samples. It offers a novel alternative to standard chromatographically interfaced solutions. Laboratories can now screen, compare and confirm virtually any sample, by introducing it directly to a mass spectrometer in a matter of seconds.
• No chromatography required.
• Avoid time consuming method development.
• Minimal sample preparation.
This reference notebook of direct sample analyses, highlights the performance characteristics of the AxION DSA. It features a diverse selection of applications, sample types and results across a broad array of industries.
The AxION DSA system will change the way you work by providing results-driven decisions in seconds.
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3www.perkinelmer.com/DSA
Index of Applications
• Atrazine in Water ................................................................................................................................................................14
• Carbamates in River Water ..................................................................................................................................................15
• Insecticide on Mosquito Nets ..............................................................................................................................................16
• Personal Care Products in River Water ................................................................................................................................. 17
environmental
• Limonene in Orange Peel ....................................................................................................................................................18
• Parabens in Lotions .............................................................................................................................................................19
chemical
Food and BeveraGe• Capsaicin and Dihydrocapsaicin in Peppers ...........................................................................................................................4
• Curcumins in Turmeric Powder ..............................................................................................................................................5
• Fungicide on Lemon Peel ......................................................................................................................................................6
• Fungicides on Oranges ..........................................................................................................................................................7
• Malathion on Peaches ...........................................................................................................................................................8
• Melatonin in Lazy Cake® .......................................................................................................................................................9
• Propanethiol S-oxide in Onions ...........................................................................................................................................10
• Resveratrol in Red Wine ...................................................................................................................................................... 11
• Sterols in Olive Oil ............................................................................................................................................................... 12
• Sweeteners in Diet Cola ...................................................................................................................................................... 13
• Components of Multivitamin Powder ..................................................................................................................................22
nUtraceUtical
ForenSicS• Date Rape Drug in Cola .......................................................................................................................................................20
• Testosterone in Steroid Powder ...........................................................................................................................................21
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TOF MS
Direct Sample analySiS
No sample preparation
Easy to use
Semi-quantitative
• Thesignalintensitiesprovidedtherelativeamountsofcapsaicinanddihydrocapsaicininthepeppers
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• Capsaicinanddihydrocapsaicinarethecompoundsresponsibleforpepperhotness
• 1cm3piecesofcubanelle,chilli,andjalapeñopeppersweredirectlyanalyzed
Food and Beverage: Capsaicin and Dihydrocapsaicin in Peppers
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TOF MS
Direct Sample analySiS
No sample preparation
Easy to use
Complete characterization
• DSA/TOFanalysisofturmericpowderdetectsall3curcuminoidswithhighmassaccuracy
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• Curcuminsaretheactiveingredientinturmericpowderusedforfoodflavoring,dyeing,andpossiblemedicalbenefits
• Turmericpowderwasdirectlyanalyzedinaglasscapillary
Food and Beverage: Curcumins in Turmeric Powder
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TOF MS
Direct Sample analySiS
Outstanding sensitivity
Fast accurate mass
Assures food safety
• Residualimazalilwasconfirmedwithhighmassaccuracy
• Theanalysiswasperformedin15secondswithminimalsamplepreparationandexternalcalibration
• Pesticidessuchasimazalilareoftenusedtoprotectfruitsandvegetablesfrominsects
• Awashed1cm3pieceoflemonpeelwasextractedwithmethanolandanalyzed
Food and Beverage: Fungicide on Lemon Peel
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7www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
No sample preparation
Easy to use
Assures food safety
• DSA/TOFanalysisdetectedthepresenceoftwofungicidesontheorangeskin
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• Tracesoffungicidesonourfoodposeapotentialrisktohumanhealth
• Aswabbingwithmethanolfromanorangeboughtatagrocerystorewasanalyzed
Food and Beverage: Fungicides on Oranges
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TOF MS
Direct Sample analySiS
Easy to use
Low detection limits
Fast, accurate mass
• DSA/TOFanalysisoftheswabsampleconfirmedmalathion,withhighmassaccuracy,at800timeslowerthantheEPAtolerancelevel
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• Malathionisacommonlyusedinsecticide.TheEPAtolerancelevelformalathiononpeachesis8000ng/g
• Apeachsurfacewasspikedwith10ng/gmalathion,thenswabbedwithmethanolandanalyzed
Food and Beverage: Malathion on Peaches
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TOF MS
Direct Sample analySiS
No sample preparation
Confirms active ingredient
Rapid analysis
• Directanalysisconfirmstheactiveingredientmelatoninwithhighmassaccuracy
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• LazyCakes®,marketedas“relaxationbrownies”arelacedwiththesleepaidmelatonin
• Thepackagingiskid-friendlyandchildrenhaveeatenthemandfallenasleep
• Apieceofcakewasanalyzedtodeterminetheingredientthatwascausingsleepiness
Food and Beverage: Melatonin in Lazy Cake®
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10www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
No sample preparation
Easy to use
Fast, accurate mass
• Withnosamplepreparation,DSA/TOFanalysisoftheonionsampledetectedPropanethiolS-oxidewithhighmassaccuracy
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• PropanethiolS-oxideinonionsisknowntocausetheeye’stotear(whencuttingtheonion)
• 1cm3pieceofonionwasdirectlyanalyzed
Food and Beverage: Propanethiol S-oxide in Onions
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11www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
Easy to use
Increased sensitivity
Rapid analysis
• DSA/TOFanalysisinTrapPulse™modeof1uLofredwine(onamesh)providestherequiredsensitivityfordetectionandconfirmationofresveratrol
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• Resveratrolisnaturallyoccurringpolyphenolpresentingrapeskinandseeds
• Itisanantioxidantthoughttobepresentinhigherlevelsinredwineandlinkedtomanyhealthbenefits
Food and Beverage: Resveratrol in Red Wine
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TOF MS
Direct Sample analySiS
Easy to use
Comparison of products
Rapid analysis
• Oliveoilscanrapidlybecomparedfordetectionofbeneficialsterols
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• Somesterolsinoliveoilarethoughttodecreasebloodcholesterol
• Saponifiedoliveoilwasdirectlyanalyzedforsterols
• DSA/TOFanalysisdetectsthebeneficialsterolsinoliveoilwithminimalsamplepreparation
Food and Beverage: Sterols in Olive Oil
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13www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
Easy to use
Component analysis
No sample preparation• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• Artificialsweetenersareoftenusedindietcola
• Sweetenersindietcolawasdirectlyanalyzedwithoutanysamplepreparation
• DSA/TOFanalysisconfirmssweetenersusedindietcolaaswellasotheradditiveswithaccuratemass
Food and Beverage: Sweeteners in Diet Cola
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14www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
Environmental: Atrazine in Water
Results in seconds
Increased sensitivity
Confirms water safety
• DSA/TOFanalysisof2uLofwateronameshconfirmspresenceofatrazinewithamassaccuracy<1ppm
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• UseofTrapPulse™modeincreasessensitivity8foldandgivesgreatermassaccuracy
• Thepesticideatrazineisacontaminantofsurfaceanddrinkingwater
• Themaximumlevelofatrazineinwaterhasbeensetas0.003mg/L
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TOF MS
Direct Sample analySiS
Environmental: Carbamates in River Water
No complex method development
Fast, accurate mass
Ensures water quality
• DSA/TOFanalysisconfirmspresenceofcarbamates,withhighmassaccuracy
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• 10ppbspikedriversamplessimplyfilteredandpipettedontoamesh
• Carbamatesareknowncontaminantsofriverwater,EPAmaximumconcentrationof10ug/L
• ExistingprotocolsrequirelengthySPEworkupfollowedbyLC,LC/MSorGC/MSanalysis
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16www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
Environmental: Insecticide on Mosquito Nets
Results in seconds
Assures product authenticity
Protects consumers
• DSA/TOFanalysisofanetsectionconfirmspresenceofdeltamethrinattherequiredleveltobeeffective
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• ExistingprotocolsrequireextractionfollowedbyHPLCorGC/MSanalysis
• DeltamethrinisaWHOapprovedinsecticidefortreatmentofmosquitonets
• Treatednetscommandahigherpricebutsomesuppliersomittheinsecticide
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17www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
No complex method development
Comprehensive analysis
Ensures water quality
• 10ppbspikedriversamplessimplyfiltered,10mMammoniumformateadded,andpipettedontoamesh
• DSA/TOFanalysisconfirmspresenceofpersonalcareproductsandpesticides,withhighmassaccuracy
• Theanalysiswasperformedin15secondswithminimalsamplepreparationandexternalcalibration
• Personalcareproductsposearisingconcernasacontaminantinpostwatertreatmentriverwaters
• ExistingprotocolsrequirelengthySPEworkupfollowedbyLC,LC/MSorGC/MSanalysis
Environmental: Personal Care Products in River Water
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TOF MS
Direct Sample analySiS
Easy to use
Compound confirmation
No sample preparation
• Limoneneismeasuredinorangepeelwithdirectanalysisandaccuratemassconfirmation
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• Limoneneisamoleculethatgivesorangestheirstrongsmellandisoftenusedincleaningproductsandaprecursorforchemicalsynthesis
• Samplepreparationandmethoddevelopmentforanalysisoflimoneneislengthyandtimeconsuming
Chemical: Limonene in Orange Peel
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19www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
Easy to use
Complete characterization
No sample preparation
• DSA/TOFdirectanalysisoflotionconfirmsparabensinlotionwithaccuratemass
• Theanalysiswasperformedin15secondswithminimalsamplepreparationandexternalcalibration
• Parabensareaclassofchemicalsusedinpreservationofcosmetics
• Samplepreparationandmethoddevelopmentofparabensislengthyandtimeconsuming
Chemical: Parabens in Lotions
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20www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
No sample preparation
Low detection limits
Fast, accurate mass
• Withnosamplepreparation,DSA/TOFanalysisofthecolasampledetectedthedrugwithhighmassaccuracy
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• Theminimumlevelofthedaterapedrug,Gamma-HydroxyButyricAcid,whichaffectshumansis1mg/mL
• Acolasamplewasspikedwiththedrugat0.1mg/mL,10-foldlessthantheminimumdetectionlimit
Forensics: Date Rape Drug in Cola
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21www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
Easy to use
Confirms compound identity
Fast, accurate mass
• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration
• Testosteroneisananabolicsteroidthatcanbeusedtoenhancephysicalperformanceinathletes
• Testosteronepowderwasdirectlyanalyzedinaglasscapillary
• Confirmationoftestosteronewasachievedinsecondswithhighmassaccuracy
Forensics: Testosterone in Steroid Powder
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22www.perkinelmer.com/DSA
TOF MS
Direct Sample analySiS
No sample preparation
Complete component analysis
Confirms product authenticity
• DSA/TOFanalysisofEmergen-C®powderinaglasscapillarydetectscomponentswithahighmassaccuracy
• Emergen-C®isadietarysupplementdesignedtosupporthealthymetabolicfunction
• Analysisinbothpositiveandnegativemodesensurescompleteproductcharacterization
Nutraceuticals: Components of Multivitamin Powder
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