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Date:

Xmatrx® Infinity – Infinite Possibilities…

BioGenex

2016

BioGenex

Intelligent & Flexible System

AutomateAnySlide-basedassay

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IHC ISH/CISH SpecialStains

FISH Mul3plexing InSituPCR

IF miRNA-ISH

Xmatrx® INFINITY – Fully Automated System

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•  Fullyautomated,totalwalkawaysystemfrombakingthroughfinalcoverslip

•  100slides/day,60slidesin8hourshiKand40slidesinovernightrun

•  Mul3-formatspecimenprocessing:Frozen,FFPE,CellPrepara3ons,Smears&FNAs

•  40independentprotocolssimultaneously

Stain More – Consume Less

•  Reducedreagentconsump3onandwastegenera3onbyupto90%

•  Allnon-toxicEco-friendlyreagents

•  Slideprocessingop3ons:Random,Con3nuousandSTATforefficiency

•  Systemallowsuseofany3edpartyreagents

Xmatrx® Enabling Technologies and Advantages

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Ø Proprietarycoverslipmechanism•  ProprietarycoverslipmechanismAllnon-toxic

Eco-friendlyreagents

Ø Reac3onmicro-chamberwithmicro-fluidics•  Reducesreagentconsump3onto5-80µL/slide

•  Allowsevendistribu3onofreagentsforuniformstainingwithoutedgeeffect

•  Oilsealmechanismpreventssampledryingduringprolongedincuba3onsathightemperatures(FISH,ISH,insituPCR

Xmatrx® Enabling Technologies and Advantages

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Ø ReagentDispensingSystem•  Disposablepipe_e3psallowmul3pletechnologieson

thesamepla`ormwithoutcrosscontamina3on

Ø ThermocyclerswitheXactTMtemperaturecontrol•  Allowsaccuratetemperaturecontrolfromambient

temperatureto105°C(±0.5)

•  40independentunitsallowrunning40differentprotocolsatthesame3me

•  Allowsfasthea3ngandcoolingforinsituPCR

Ambient95°C<2min

Xmatrx® Enabling Technologies and Advantages

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Ø LiquidLevelSensor•  Allowsaccuratereagentdispensing

Ø IntelligentOpenSystemSoKware•  Intui3ve,flexibleandeasytousesoKwareenables

quicklearningandeasysetup

Differentiation: Xmatrx® Infinity vs. other instruments

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Automation XmatrxInfinity LeicaBond BenchMarkDiscovery Omnis

Company BioGenex LeicaBioSystems Ventana(Roche) Dako(Agilent)

SlideCapacity 40 30 30 60

Minimumprobevolumerequired 10µl 150µl 100µl 200µl

Baking Yes Yes Yes No

DeWax Yes Yes Yes Yes

Retrieval Yes Yes Yes Yes

Staining Yes Yes Yes Yes

Counterstain Yes Yes Yes Yes

De-hydrate Yes No No No

Clearing Yes No No No

Coverslip Yes No No No

ReagentDispensing Disposabletips RinsedProbetip Dispos.dispensers RinsedProbetip

Dimensions(W/H/D) 46/59/29in 31/54/31in 35/60/26 57/60/31

Weight(lbs) 400 542 650 1323

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Customer Testimonials & Selected Publications

Dr.SharakhovVirginiaTech.

In the fall of 2011 Dr. Sharakhov’slaboratory installed an Xmatrx Infinitysystemtophysicallymapchromosomesofvarious invertebrate species usingautomatedfluorescentinsituhybridiza3on

Due to manual staining processes, thecenter was handling approximately 5000slidesperyear. However,thenecessityofIHC staining for the diagnosis of variousdiseasesincreaseseveryday

Beingacancerresearchlaboratory,theBCRLis accustomed to immunohistochemistry(IHC) and mul3plex IHC to analyze, confirmandlearnaboutthemyriadofinterac3ons

David’s laboratoryhasawidemenuof testsavailable and offers services to clients of allsizes.BioGenexisproudtobeapartofsuchalargeandprominentacademichospital

St.PetersburgRussia.

BCRLPhiladelphia.

Dr.DavidDavisDenver.

SelectedPublica3ons•  Abbo4Molecular(BloodCancerJ.)LossinMCL-1func3onsensi3zesnon-Hodgkin’slymphomacell

linestotheBCL-2-selec3veinhibitorvenetoclax(ABT-199)

•  JohnsHopkinsUniversity(JNeurovirol.)Mechanismsofminocycline-inducedsuppressionofsimianimmunodeficiencyvirusencephali3s:inhibi3onofapoptosissignal-regula3ngkinase1

•  VirginiaTech(J.Vis.Exp.)High-throughputPhysicalMappingofChromosomesusingAutomatedinsituHybridiza3on

•  AlbanyMedicalCollege(Hum.Pathol.)Lossofclaudins-1and-7andexpressionofclaudins-3and-4correlatewithprognos3cvariablesinprosta3cadenocarcinomas

•  GeorgetownUniversityHospital(CancerRes.)Prognos3csignificanceofimmunohistochemicalexpressionofGATA4prostate(PC)andendometrial(EC)carcinomas

Tes3monials

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Uniqueness

Ø Fullyautomatedsystem•  BakingtoFinalCoverslip

Ø Mul3pleapplica3onsfromIHC,ISH,SStoFISH•  Eliminaterequirementtopurchasemul3pleinstrumentsfor

differenttechnologies

•  Savecost/labspace

Ø Micro-Chamber/Coversliptechnologies•  Eco-friendlyandCostsavings–upto90%reducedreagent

consump3onandwastegenera3on

Ø FlexibleEasy-to-UseOpenSystemSoKware•  Factorytemplateprotocolsoreasilycustomizeduser

protocols

•  RegulatorycompliantwithUS-FDAandIVD-CEmarkandmeetsISOqualitystandards

TIME

COST

QUALITY

BioGenex Mission: “Tobecomeaglobalmolecularmedicineleaderprovidingaffordablesolu3onsforlifescienceresearchandpersonalizedmedicine”

Headquarters

•  Silicon Valley, California

Facilities & Offices •  Hyderabad, India •  Milan, Italy •  Shanghai, China

Distributors

•  70+ across 47 countries

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BioGenex IHC

BreastCancerPanel

CathepsinD EstrogenReceptor Cytokera3n5

E-cadherin EGFR GCDFP-15

MUC4 CK14 ProgesteroneReceptor

>400An3bodies

Detec3onSystems

DeWaxandAn3genRetrieval

ControlSlides

Ancillaries

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ISH Probes and kits insituHybridiza3on(ISH)allowsdetec3onofspecificnucleicacidsequences(genes)intheircellularenvironment.

HPV16/18

ALUIICYCLIND1KAPPA LAMBDA

EBER ISHStainingKitsformanual&automa3on

Over 150 microRNA - ISH Probes

Has-miR148b

Has-miR222Hsa-miR155Hsa-miR1 Hsa-miR146a

Has-miR328 Has-miR30e Has-miR451

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Special Stains

Ø SpecialStains(SS)•  Arechemicaldyestainsthatlocalizetomicrobesfound

in3ssueandtospecific3ssuetypesandareusedprimarilytoassistinthediagnosisofinfec3ousdiseasesandalsocancer

Ø 30specialstainingkitsavailable•  Masson'sTrichome•  SafraninOStainingProtocol

•  Toluidinebluestaining

•  vonKossaStaining

•  AlizarinRedStainingKit

•  AzureAstaining

A.MucicarmineinIntes3neB.Re3culin-NuclearFastRedC.Masson’sTrichromeinSkin

A.IronStainB.PASinLiverC.Elas3ninSkin

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eFISH Probes and Kits

Ø Probes•  65probescurrentlyavailable

•  SolidTumors:26

•  Hematology:20

•  Enumera3on:19

•  Single,double,triplecolors

•  Plantoincreaseprobeofferingto200bytheendof2015

Ø  Visualiza3onkitsfor•  FFPE

•  Cytosamples

•  Automa3oncompa3ble

TP53/CEN17FP-062

Normal(leK)andTP53dele3on(right)CLL

CEN7/EGFRFP-040

CEN7/EGFRamplifica3onNon-smallcelllungcancer

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In Situ PCR Automation with Xmatrx®

•  Supersensi3ve:ISP1-2copies/cellvsCISH

>10copies/cell

•  Producereliableandreproducibleresults

•  Veryspecific:nostaininginthenega3ve

controlsamples.

•  Fullyautomated:canbefinishedbyXmatrx

HPV18inFFPE3ssue

CervicalCarcinomashowingPosi3veHPV18SignalbyInSituPCR

HPV18inHeLaCellLinedemonstra3ngHighSensi3vityofInSituPCR

InSituPCR(20Cycles) InSituPCRHybridiza3on

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ThankYouPleasevisitwww.biogenex.comformoredetailsonourproductpor`olio

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