automated dna sequencing ap biology fall 2010. automated dna sequencing currently, laboratories…

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Automated DNA Sequencing  The machine builds DNA molecules but uses eight kinds of bases Four normal Four that are modified to fluoresce in laser light

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Automated DNA Sequencing

AP BiologyFall 2010

Automated DNA Sequencing Currently, laboratories use automated

DNA sequencing to determine the unknown sequence of bases in a DNA sample

Page 3: Automated DNA Sequencing AP Biology Fall 2010. Automated DNA Sequencing Currently, laboratories…

Automated DNA Sequencing The machine builds DNA molecules but

uses eight kinds of basesFour normalFour that are modified to fluoresce in laser

light

Page 4: Automated DNA Sequencing AP Biology Fall 2010. Automated DNA Sequencing Currently, laboratories…

Automated DNA Sequencing When a modified base is incorporated,

DNA synthesis is haltedThis produces tagged fragments of different

lengths

Page 5: Automated DNA Sequencing AP Biology Fall 2010. Automated DNA Sequencing Currently, laboratories…

Page 6: Automated DNA Sequencing AP Biology Fall 2010. Automated DNA Sequencing Currently, laboratories…

Gel Electrophoresis The automated DNA sequencer

separates the sets of fragments by gel electrophoresis

Page 7: Automated DNA Sequencing AP Biology Fall 2010. Automated DNA Sequencing Currently, laboratories…

Gel Electrophoresis The “tag” base at the end of each

fragment in the set is identified by the laser beam

The computer program in the machine assembles the information from all the nucleotides in the sample to reveal the entire DNA sequence

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